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J Microbiol ; 46(6): 709-19, 2008 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-19107402

RESUMEN

To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.


Asunto(s)
Baculoviridae/metabolismo , Sistemas de Lectura Abierta/genética , Virus del Síndrome Respiratorio y Reproductivo Porcino/clasificación , Virus del Síndrome Respiratorio y Reproductivo Porcino/genética , Recombinación Genética , Proteínas Estructurales Virales/metabolismo , Animales , Baculoviridae/genética , Secuencia de Bases , Células Cultivadas , Corea (Geográfico) , Datos de Secuencia Molecular , Filogenia , Síndrome Respiratorio y de la Reproducción Porcina/virología , Virus del Síndrome Respiratorio y Reproductivo Porcino/aislamiento & purificación , Virus del Síndrome Respiratorio y Reproductivo Porcino/metabolismo , Alineación de Secuencia , Spodoptera/virología , Porcinos , Proteínas Estructurales Virales/genética
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