Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Filtrar
Más filtros

Banco de datos
Tipo del documento
Intervalo de año de publicación
1.
Nucleic Acids Res ; 33(Database issue): D471-5, 2005 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-15608240

RESUMEN

The Mouse Genome Database (MGD) forms the core of the Mouse Genome Informatics (MGI) system (http://www.informatics.jax.org), a model organism database resource for the laboratory mouse. MGD provides essential integration of experimental knowledge for the mouse system with information annotated from both literature and online sources. MGD curates and presents consensus and experimental data representations of genotype (sequence) through phenotype information, including highly detailed reports about genes and gene products. Primary foci of integration are through representations of relationships among genes, sequences and phenotypes. MGD collaborates with other bioinformatics groups to curate a definitive set of information about the laboratory mouse and to build and implement the data and semantic standards that are essential for comparative genome analysis. Recent improvements in MGD discussed here include the enhancement of phenotype resources, the re-development of the International Mouse Strain Resource, IMSR, the update of mammalian orthology datasets and the electronic publication of classic books in mouse genetics.


Asunto(s)
Bases de Datos Genéticas , Genómica , Ratones/genética , Animales , Genes , Genoma , Genotipo , Internet , Ratones Mutantes , Fenotipo , Integración de Sistemas , Interfaz Usuario-Computador
2.
Nucleic Acids Res ; 32(Database issue): D476-81, 2004 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-14681461

RESUMEN

The Mouse Genome Database (MGD) is one component of the Mouse Genome Informatics (MGI) system (http://www.informatics.jax.org), a community database resource for the laboratory mouse. MGD strives to provide a comprehensive knowledgebase about the mouse with experiments and data annotated from both literature and online sources. MGD curates and presents consensus and experimental data representations of genetic, genotype (sequence) and phenotype information including highly detailed reports about genes and gene products. Primary foci of integration are through representations of relationships between genes, sequences and phenotypes. MGD collaborates with other bioinformatics groups to curate a definitive set of information about the laboratory mouse and to build and implement the data and semantic standards that are essential for comparative genome analysis. Recent developments in MGD discussed here include an extensive integration of the mouse sequence data and substantial revisions in the presentation, query and visualization of sequence data.


Asunto(s)
Biología Computacional , Bases de Datos Genéticas , Genoma , Ratones/genética , Animales , Genómica , Almacenamiento y Recuperación de la Información , Internet , Biología Molecular , Fenotipo , Terminología como Asunto
3.
Endocrinology ; 123(3): 1690-2, 1988 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-3402402

RESUMEN

Substantial responses in the 6-week and mature body-weights of mice occurred after 7 generations of selection for or against plasma levels of Insulin-like Growth Factor-1 (IGF-1). Plasma levels of IGF-1 were also significantly different after 7 generations of selection (high line = 85 +/- 2 ng/ml, low line = 58 +/- 2 ng/ml). The average 6-week weight in the line selected for high plasma IGF-1 was 22.5 +/- .2 g compared with 18.5 +/- .2 g in the low plasma IGF-1 line, after 7 generations of selection. The difference between lines was maintained at 20 weeks of age. These data provide further evidence for the roles of IGF-1 in the regulation of somatic growth and as a mediator of a genetic component of growth.


Asunto(s)
Factor I del Crecimiento Similar a la Insulina/genética , Ratones Endogámicos/crecimiento & desarrollo , Selección Genética , Somatomedinas/genética , Envejecimiento , Animales , Peso Corporal , Técnicas In Vitro , Factor I del Crecimiento Similar a la Insulina/sangre , Ratones
4.
Genet Res ; 53(3): 187-91, 1989 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-2767428

RESUMEN

A divergent selection experiment with mice, using plasma concentrations of insulin-like growth factor-1 (IGF-1) at 42 days of age as the selection criterion, was undertaken for 7 generations. Lines were not replicated. To obtain sufficient plasma for the IGF-1 assay, blood from four individuals was volumetrically bulked to obtain a litter mean IGF-1 concentration. This necessitated the use of between family selection. Although inbreeding accumulated in a linear fashion in each of the high, control and low lines, the rates were different for each line (3.6, 1.6 and 5.3% per generation for the high, control and low lines, respectively). As a consequence, the effects of selection and inbreeding are confounded in this experiment. Divergence between the high and low lines in plasma concentrations of IGF-1 continued steadily until generation 5. In generations 6 and 7, there was a reduced degree of divergence and this contributed towards the low realized heritability value of 0.15 +/- 0.12. Six-week liveweight showed a steady positive correlated response to selection for or against plasma concentrations of IGF-1 until generation 4 (high-low difference = 1.7 g = 12%). In generation 5, a substantial drop in 6-week liveweight in the low line relative to both the high and control lines occurred (high-low difference, 3.9; g, 25%). This difference was maintained until generation 7.(ABSTRACT TRUNCATED AT 250 WORDS)


Asunto(s)
Factor I del Crecimiento Similar a la Insulina/genética , Somatomedinas/genética , Animales , Peso Corporal , Femenino , Variación Genética , Factor I del Crecimiento Similar a la Insulina/sangre , Masculino , Ratones , Ratones Endogámicos , Selección Genética
5.
J Reprod Fertil ; 101(1): 9-14, 1994 May.
Artículo en Inglés | MEDLINE | ID: mdl-8064697

RESUMEN

The role of insulin-like growth factor I in the regulation of fetal growth was investigated in two lines of mice selected for high or low concentrations of this factor in plasma. In Expt 1, females from each line were mated with males of the reciprocal line to generate fetuses of equivalent genotype. Females with low concentration of the factor in plasma exhibited the typical negative relationship between mean fetal mass and litter size (b = -0.032 +/- 0.006 g per fetus, P < 0.01). However, dams of the line with high concentrations of the factor did not exhibit this relationship (b = -0.004 +/- 0.006 g per fetus), despite the fact that they had 26% larger litters (P < 0.05) at a common maternal body mass. This difference in maternal constraint apparently reflects a greater capacity for nutrient transfer to the fetuses in the dams with more insulin-like growth factor I in plasma, as suggested by the absence of a relationship between mean placental mass and mean fetal mass in that line. In Expt 2, the effect of fetal genotype for insulin-like growth factor I was investigated by transferring embryos of the two lines into females of an unrelated strain. Fetuses from the line with high concentrations of the factor in plasma were heavier at term (1.51 versus 1.37 g, pooled SE = 0.05 g, P < 0.05) than fetuses from the line with low concentrations in plasma. It is therefore concluded that fetal growth is influenced by both the maternal and fetal genotypes for insulin-like growth factor I, but in qualitatively different manners.


Asunto(s)
Desarrollo Embrionario y Fetal/genética , Feto/fisiología , Factor I del Crecimiento Similar a la Insulina/genética , Animales , Transferencia de Embrión , Desarrollo Embrionario y Fetal/fisiología , Femenino , Sangre Fetal/química , Feto/anatomía & histología , Genotipo , Factor I del Crecimiento Similar a la Insulina/análisis , Factor I del Crecimiento Similar a la Insulina/fisiología , Tamaño de la Camada , Ratones , Ratones Endogámicos , Placenta/anatomía & histología , Embarazo
6.
Aust J Biol Sci ; 40(3): 287-93, 1987.
Artículo en Inglés | MEDLINE | ID: mdl-3442513

RESUMEN

Three experiments were undertaken to examine the degree and causes of variation in plasma concentrations of insulin-like growth factor-1 (IGF-1) in mice. The relationship between IGF-1 concentrations and liveweight was also examined. In all three experiments, a number of non-genetic factors were found to contribute significantly to the variation in IGF-1 concentrations, the most important of these being sex and litter size. In one experiment, where pups from 16 litters were cross-fostered to avoid the confounding of maternal and direct genetic effects, a heritability of 0.40 +/- 0.27 was estimated for plasma IGF-1 concentration at 35 days of age. To examine further the existence of genetic variation in plasma concentrations of IGF-1 and the genetic covariation between plasma IGF-1 levels and other body traits, a selection experiment with mice has been initiated. Moderate to strong phenotypic correlations between IGF-1 concentrations and weight at an early age have been found in all three experiments.


Asunto(s)
Peso Corporal , Factor I del Crecimiento Similar a la Insulina/sangre , Somatomedinas/sangre , Envejecimiento/sangre , Animales , Factor I del Crecimiento Similar a la Insulina/genética , Ratones , Ratones Endogámicos , Fenotipo , Caracteres Sexuales
7.
Acta Endocrinol (Copenh) ; 121(5): 686-90, 1989 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-2588939

RESUMEN

A study was undertaken to investigate the role of testosterone in regulating growth and circulating levels of insulin-like growth factor-I in male mice from lines divergently selected on the basis of plasma IGF-I. Controls of each lines were sham-operated at 10 days of age and treated with peanut oil from day 14 to day 70. A second group, which was castrated at 10 days and treated with testosterone enanthate (0.5 micrograms.(g body weight)-1.day-1) from day 14 to 70, did not differ from controls in body weight but had higher plasma IGF-I concentrations. Delaying testosterone therapy until day 42 in a third group retarded growth, with body weights being significantly lower than those of other two groups from days 35 to 56. However, plasma IGF-I levels in this group were not different from those of controls. Effects of line and treatment were additive. It is concluded that the greater pubertal growth of high-line compared to low-line males is not due to greater stimulation of circulating IGF-I by testosterone. Furthermore, testosterone does not appear to influence pubertal growth by acting on circulating levels of IGF-I.


Asunto(s)
Factor I del Crecimiento Similar a la Insulina/inmunología , Maduración Sexual/efectos de los fármacos , Somatomedinas/inmunología , Testosterona/análogos & derivados , Animales , Peso Corporal/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos , Orquiectomía , Aceite de Cacahuete , Aceites de Plantas/administración & dosificación , Testosterona/farmacología
8.
J Reprod Fertil ; 87(1): 349-53, 1989 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-2621706

RESUMEN

Reproductive performance, mammary gland weight and plasma concentrations of insulin-like growth factor-1 (IGF-1) were examined in 18-day-pregnant mice from lines divergently selected on the basis of plasma IGF-1 concentration. Females of the high IGF-1 (H) line were 14% heavier than those of the low IGF-1 (L) line at mating but did not differ in conception rate during a 15-day mating period. H-line females produced significantly larger litters by an average of 1.5 fetuses (19%), heavier fetuses (7%), greater total fetal weight (30%), heavier placental discs (15%), greater total placental weight (35%) and heavier mammary glands (18%). Plasma IGF-1 values were 12% greater in H-line than L-line females at Day 19 of gestation but the line difference was not significant. It is concluded that differences between the lines in litter size and mammary gland weight are most likely due to differences in maternal bodyweight (which are in turn a consequence of selection for plasma IGF-1 at puberty). Whether the difference in fetal weight is a function of fetal capacity to grow in utero or ability of the dam to provide nutrients for fetal growth is yet to be determined.


Asunto(s)
Desarrollo Embrionario y Fetal/fisiología , Factor I del Crecimiento Similar a la Insulina/metabolismo , Reproducción/fisiología , Somatomedinas/metabolismo , Animales , Femenino , Tamaño de la Camada , Glándulas Mamarias Animales/anatomía & histología , Ratones , Ratones Endogámicos , Tamaño de los Órganos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA