RESUMEN
BACKGROUND: In vitro testing for food allergy may yield clinically irrelevant results due to cross-reactive carbohydrate determinants (CCD) specific immunoglobulin E (sIgE) induced by pollen exposure. The performances of 2 in vitro methods were evaluated for peanut sIgE measurement in patients allergic to grass pollen with or without subsequent allergy to peanuts. The correlation between clinically irrelevant peanut sIgE and the presence of CCD sIgE was investigated. METHODS: In vitro measurement of peanut sIgE was performed using the Pharmacia ImmunoCap system Radio Immuno Assay (RIA) and the Immulite 2000 3gAllergy system. Discrepancies between in vitro results and peanut allergy diagnosis were evaluated by measurement of CCD sIgE using bromelain and ascorbic acid oxydase (AAO). RESULTS: The sensitivity was 100% with both systems for the diagnosis of allergy to peanut (58 patients), nevertheless the specificity obtained with Immulite (73%) was better than that obtained using ImmunoCap (46%) in patients who were not allergic to peanuts, but who had a grass pollen allergy (n = 41). In 22 out of 41 patients who presented clinically irrelevant peanut sIgE results using ImmunoCAP, CCD sIgE was detected in 72% of the cases by bromelain and in 86% by AAO. In 11 patients out of 41 who presented irrelevant peanut sIgE results using Immulite, CCD sIgE was detected in 81% of the cases by bromelain and in 100% by AAO. CONCLUSION: The Immulite 2000 system had better specificity than the ImmunoCap system for accurate diagnosis of peanut allergy in patients allergic to grass pollen. CCD sIgE was identified in most of the false-positive peanut sIgE results.
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Arachis/inmunología , Inmunoensayo/métodos , Inmunoglobulina E/sangre , Hipersensibilidad al Cacahuete/diagnóstico , Polen/inmunología , Rinitis Alérgica Estacional/inmunología , Bromelaínas/análisis , Reacciones Cruzadas/inmunología , Humanos , Hipersensibilidad al Cacahuete/inmunología , Sensibilidad y EspecificidadRESUMEN
OBJECTIVES: To determine the prevalence of sensitization to lupin flour in patients consulting allergists, in order to evaluate the risk of primary and secondary allergies to lupin. METHODS: A prospective study carried out by members of the Allergy Vigilance Network, using prick-tests with a commercial lupin flour extract in patients with various allergic symptoms. The study design classified patients into four groups: peanut allergy, current atopic disease, latent atopy, no atopy. Data were collected and analysed by Network coordinators. RESULTS: Over a two-month period, 88 French and Belgian allergists tested 5,366 patients: 2,680 children and 2,686 adults aged over 16 years. Of the 2,680 children, 11.15% presented with peanut allergy. The frequency of cross-reactivity with lupin was 17.1% for patients with peanut allergy, 2.5% for children with current atopic disease and 1.7% for healthy children with latent atopy. In the 2,686 adults, peanut allergy was diagnosed in 1.86% of patients with cross-reactivity to lupin in 14.6%. Sensitization to lupin was detected in 3.7% of patients with current atopic disease and in 1.8% of those with latent atopy. CONCLUSION: The relative frequency of latent sensitisation to lupin in patients of all ages presenting with atopic disease is a new factor indicating the likelihood of an increase in primary food allergies to lupin flour. This justifies the recent decision requiring mandatory labelling of lupin, and shows the need to inform consumers who may be unaware that this ingredient is being used increasingly. Sensitization to lupin should be searched by prick-tests in any case of peanut allergy. Prick-test to lupin may be valuable whenever a food allergy is suspected when no current food allergens have been identified.
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Dermatitis Atópica/epidemiología , Dermatitis Atópica/inmunología , Hipersensibilidad a los Alimentos/epidemiología , Hipersensibilidad a los Alimentos/inmunología , Inmunoglobulina E/metabolismo , Adulto , Antígenos de Plantas/efectos adversos , Antígenos de Plantas/inmunología , Bélgica , Niño , Reacciones Cruzadas , Dermatitis Atópica/complicaciones , Dermatitis Atópica/terapia , Desensibilización Inmunológica , Femenino , Hipersensibilidad a los Alimentos/complicaciones , Hipersensibilidad a los Alimentos/terapia , Francia , Humanos , Inmunoglobulina E/inmunología , Servicios de Información , Lupinus/efectos adversos , Lupinus/inmunología , Masculino , Imitación Molecular/inmunología , Extractos Vegetales/administración & dosificación , Prevalencia , Estudios Prospectivos , Pruebas CutáneasRESUMEN
During early periods of life, modifications of the gravitational environment affect the development of sensory, neuronal and motor systems. The vestibular system exerts significant effects on motor networks that control eye and body posture as well as swimming. The objective of the present study was to study whether altered gravity (AG) affects vestibuloocular and spinal motor systems in a correlated manner. During the French Soyuz taxi flight Andromède to the International Space Station ISS (launch: October 21, 2001; landing: October 31, 2001) Xenopus laevis embryos were exposed for 10 days to microgravity (microg). In addition, a similar experiment with 3g-hypergravity (3g) was performed in the laboratory. At onset of AG, embryos had reached developmental stages 24 to 27. After exposure to AG, each tadpole was tested for its roll-induced vestibuloocular reflex (rVOR) and 3 hours later it was tested for the neuronal activity recorded from the ventral roots (VR) during fictive swimming. During the post-AG recording periods tadpoles had reached developmental stages 45 to 47. It was observed that microgravity affected VR activity during fictive swimming and rVOR. In particular, VR activity changes included a significant decrease of the rostrocaudal delay and a significant increase of episode duration. The rVOR-amplitude was transiently depressed. Hypergravity was less effective on the locomotor pattern; occurring effects on fictive swimming were the opposite of microg effects. As after microgravity, the rVOR was depressed after 3g-exposure. All modifications of the rVOR and VR-activity recovered to normal levels within 4 to 7 days after termination of AG. Significant correlations between the rVOR amplitude and VR activity of respective tadpoles during the recording period have been observed in both tadpoles with or without AG experience. The data are consistent with the assumptions that during this period of life which is characterized by a progressive development of vestibuloocular and vestibulospinal projections (i) microgravity retards the development of VR activity while hypergravity weakly accelerates it; (ii) that microgravity retards the rVOR development while hypergravity caused a sensitization, and that (iii) AG-induced changes of VR activity during fictive swimming have a vestibular origin.
Asunto(s)
Hipergravedad , Reflejo Vestibuloocular/fisiología , Raíces Nerviosas Espinales/fisiología , Natación/fisiología , Ingravidez , Xenopus laevis/crecimiento & desarrollo , Potenciales de Acción/fisiología , Envejecimiento/fisiología , Animales , Tronco Encefálico/crecimiento & desarrollo , Vías Eferentes/crecimiento & desarrollo , Femenino , Sensación de Gravedad/fisiología , Hipergravedad/efectos adversos , Larva/anatomía & histología , Larva/crecimiento & desarrollo , Neuronas Motoras/fisiología , Músculos Oculomotores/inervación , Equilibrio Postural/fisiología , Recuperación de la Función/fisiología , Vuelo Espacial , Médula Espinal/crecimiento & desarrollo , Núcleos Vestibulares/crecimiento & desarrollo , Vestíbulo del Laberinto/fisiología , Ingravidez/efectos adversos , Xenopus laevis/anatomía & histologíaRESUMEN
Body piercing and tattooing are increasingly common. As well as the risk of infection and scarring, allergic reactions are also reported. This is the first multi-centre study to assess the frequency of consultations for allergy. Of the 138 allergologists who answered our two questionnaires, 7.9% reported allergic reactions associated with body piercing and 18.9% identified allergies associated with temporary henna-based tattoos. Contact eczema, rhinitis and urticaria were related to nickel allergy. Contact eczema, generalized eczema, pruritus and edema were caused by tattoos. In 20 out of 28 cases, sensitization to para-phenylenediamine (PPD) was observed. The authors review the literature, underscoring the risk of serious allergy to PPD, the need for long-term monitoring of the risk of skin lymphocytoma, the difficulties met during treatment and the necessity of regulating tattooing and body piercing practices.
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Perforación del Cuerpo/efectos adversos , Hipersensibilidad/epidemiología , Hipersensibilidad/etiología , Tatuaje/efectos adversos , Humanos , Estudios RetrospectivosRESUMEN
Studies were carried out on the embryo of the amphibian Pleurodeles waltl to investigate the potential role of the N-linked oligosaccharides of the ectodermal cell membrane in the neural induction process. Glycopeptidase F (GPase F) was used to cleave N-linked oligosaccharides on presumptive ectoderm. Removal of oligosaccharide moieties from ectoderm membrane glycoconjugates completely inhibited natural neural induction in vitro. On the other hand, Swainsonine (Sw) and 1-deoxynojirimycin (dNM), specific inhibitors of enzymes involved in glycosylation, provoked strong and persistent changes in the structure of the N-linked oligosaccharides of presumptive ectoderm but did not prevent neuralisation of treated ectoderm. We conclude that N-linked carbohydrates are implicated in the phenomenon of neural induction. However, the structural integrity of N-linked carbohydrates of target tissue is not itself critical in this process. The existence of specific carbohydrates on presumptive ectoderm was still questioned as receptors of neural signal.
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Ectodermo/fisiología , Sistema Nervioso/embriología , Oligosacáridos/metabolismo , Pleurodeles/embriología , 1-Desoxinojirimicina , Alcaloides , Amidohidrolasas , Animales , Ectodermo/química , Glucosamina/análogos & derivados , Manosidasas/antagonistas & inhibidores , Péptido-N4-(N-acetil-beta-glucosaminil) Asparagina Amidasa , SwainsoninaAsunto(s)
Quiste Coloide/complicaciones , Quiste Coloide/diagnóstico , Papiledema/diagnóstico , Papiledema/etiología , Adulto , Quiste Coloide/cirugía , Servicios Médicos de Urgencia/métodos , Humanos , Hipertensión Intracraneal/complicaciones , Hipertensión Intracraneal/diagnóstico , Imagen por Resonancia Magnética , Masculino , Procedimientos Neuroquirúrgicos , Papiledema/cirugíaRESUMEN
The CELIMENE space experiment (CELulles en Impesanteur: Muscle Et Neurone Embryonnaires) was devoted to the study of the influence of gravity on the differentiation, the organisation and the maintenance of the highly specialised nervous system and muscular system. CELIMENE was carried out during the first flight of the IBIS hardware (Instrument for BIology in Space) with the fully automatic space mission PHOTON 10 in February 1995. Using the amphibian Pleurodeles waltl as a vertebrate model, in vitro experiments involved immunocytochemical detection of glial-, neuronal- and muscle-specific markers, and neurotransmitters in cells developed under conditions of microgravity compared with 1g controls, on-board and on the ground. We observed that the altered gravity did not disturb cell morphogenesis or differentiation.
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Músculos/fisiología , Neuroglía/fisiología , Neuronas/fisiología , Pleurodeles/embriología , Vuelo Espacial , Ingravidez , Animales , Diferenciación Celular/fisiología , Células Cultivadas , Ectodermo/citología , Desarrollo Embrionario , Músculos/citología , Neuroglía/inmunología , Neuronas/inmunologíaRESUMEN
Pleurodeles waltl (amphibian, Urodele) is an appropriate biological model for space experiments on a vertebrate. One reason for interest in this animal concerns the study of the effects of absence of gravity on embryonic development. First, after mating (on Earth) the females retain live, functional sperm in their cloacum for up to 5 months, allowing normal in vivo fertilisation after hormonal stimulation. Second, their development is slow, which allows analyses of all the key stages of ontogenesis from the oocyte to swimming tailbud embryos or larvae. We have performed detailed studies and analyses of the effects of weightlessness on amphibian Pleurodeles embryos, fertilised and allowed to develop until the swimming larvae stage. These experiments were performed in space during three missions on the MIR-station: FERTILE I, FERTILE II and NEUROGENESIS respectively in 1996, 1998 and 1999. We show that in microgravity abnormalities appeared at specific stages of development compared to 1g-centrifuge control embryos and 1g-ground control embryos. In this report we describe abnormalities occurring in the central nervous system. These modifications occur during the neurulation process (delay in the closure of the neural tube and failure of closure of this tube in the cephalic area) and at the early tailbud stage (microcephaly observed in 40% of the microgravity-embryos). However, if acephalic and microcephalic embryos are not taken into account, these abnormalities did not disturb further morphological, biochemical and functional development and the embryos were able to regulate and a majority of normal hatching and swimming larvae were obtained in weightlessness with a developmental time-course equivalent to that of 1g-centrifuge control embryos (on the MIR station) and 1g-ground control embryos.
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Sistema Nervioso Central/embriología , Modelos Animales , Pleurodeles/fisiología , Vuelo Espacial , Ingravidez , Animales , Astrocitos/fisiología , Sistema Nervioso Central/anomalías , Sistema Nervioso Central/crecimiento & desarrollo , Sistema Nervioso Central/fisiología , Colina O-Acetiltransferasa/metabolismo , Proteínas del Citoesqueleto/metabolismo , Oído/embriología , Embrión no Mamífero/anomalías , Embrión no Mamífero/embriología , Embrión no Mamífero/fisiología , Desarrollo Embrionario , Ojo/embriología , Femenino , Larva/crecimiento & desarrollo , Larva/fisiología , Masculino , Actividad Motora , Pleurodeles/anomalías , Pleurodeles/embriología , Pleurodeles/crecimiento & desarrollo , Ácido gamma-Aminobutírico/metabolismoRESUMEN
The study of the influence of weightlessness on fertilization and embryonic development of a vertebrate is of importance in the understanding of basic embryogenesis and in the preparation of the future exploration of space. Accordingly, specific hardware was designed to perform experiments on board the MIR space station with an amphibian vertebrate model, taking into account the biological requirements and the multiple constraints of a long-term mission. This paper describes the biological uses and presents the technological specifications of the device developed under CNES management. The hardware was adapted to and is compatible with biological requirements as confirmed by three experiments performed in space on board the orbital MIR station.
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Vivienda para Animales , Modelos Animales , Pleurodeles/embriología , Vuelo Espacial/instrumentación , Ingravidez , Animales , Desarrollo Embrionario , Diseño de Equipo , Estudios de Factibilidad , Femenino , Fertilización/fisiología , Masculino , Pleurodeles/crecimiento & desarrollo , Nave Espacial/instrumentaciónRESUMEN
INTRODUCTION: The management in schools of children with life-threatening or chronic diseases has led to the development of Individual Reception Projects (IRP) since 1993, notified in the state education ministry's circular letter. The aim of this study was to assess the status of such IRPs in France and the French Overseas Territories in the management of food allergy risks. METHOD: The regional departments of educational promotion were contacted and the survey was run between May and June, 2002. The items of the questionnaire were: the number of IRPs for anaphylactic emergencies, details on the allergies themselves, an overview of the eventual existence of dialogue structures among school physicians, heads of schools, allergists, treatment prescribers and emergency protocols. RESULTS: The total survey revealed 7482 IRP for anaphylactic reactions to food (14% of the total IRPs). The frequency of IRPs varied greatly depending on the regions. The Paris area represented 36.3% of the total. The Lorraine area: 5.6%, the Haute-Garonne: 5%, and the Bouches-du-Rhône: 3.3%. The IRPs represented 0.002 (Ardèche) to 0.33% (Alpes-de-Haute-Provence) of the school attending population. The mean level was of 0.065%; 71.6% of the IRPs concerned the 1st grade (1 IRP for 1091 children). In decreasing frequency, the allergens concerned were: peanuts, eggs, dried nuts, fish, dried peas and milk. Dialogue structures were established in 38 regions. DISCUSSION: The IRPs have increased 4-fold since the circular letter in 1999. The enhanced prevalence of numerous (new) allergies to dried nuts and leguminous plants has been emphasized, and peanut allergies have been confirmed. The complexity of managing the IRPs and need for information and training of state education and municipal staff requires the further development of dialogue structures, which are still clearly insufficient.
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Anafilaxia , Servicios Médicos de Urgencia , Hipersensibilidad a los Alimentos , Adolescente , Factores de Edad , Alérgenos , Niño , Francia , Humanos , Paris , Factores de Riesgo , Encuestas y CuestionariosRESUMEN
The Allergo-vigilance Network including 200 French allergologists and 28 European ones, has three objectives: to index the cases of lethal or prelethal anaphylaxis, to evaluate the prevalence of food allergies, clinical pictures and allergens, and to implement the post-marketing surveillance of the allergic risk of novel foods. The first survey dealing with life-threatening food allergies leads to an estimation of 15,000 to 30,000 reactions per year in France. The AVN could be a helpful tool for public health organizations.
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Anafilaxia/epidemiología , Bases de Datos Factuales , Hipersensibilidad a los Alimentos/epidemiología , Encuestas Epidemiológicas , Edema Laríngeo/epidemiología , Vigilancia de la Población , Adulto , África del Norte/epidemiología , Anafilaxia/etiología , Arachis/efectos adversos , Argentina/epidemiología , Asma/epidemiología , Asma/etiología , Niño , Reacciones Cruzadas , Proteínas en la Dieta/efectos adversos , Europa (Continente)/epidemiología , Francia/epidemiología , Frutas/efectos adversos , Humanos , Edema Laríngeo/etiología , Hipersensibilidad al Látex/epidemiología , Martinica/epidemiología , Nueces/efectos adversos , Prevalencia , Reunión/epidemiología , Estados Unidos/epidemiologíaRESUMEN
In lower vertebrates, gravity deprivation by orbital flights modifies the vestibuloocular reflex. Using the amphibian Xenopus laevis, the experiments should clarify to which extent macular structures of the labyrinth are responsible for these modifications. In particular, the shape of otoconia and number and size of sensory macular cells expressing CalBindin were considered. CalBindin is common in mature sensory cells including vestibular hair cells and is probably involved in otoconia formation. Two developmental stages were used for this study: stage 26/27 embryos, which were unable to perform the roll-induced vestibuloocular reflex (rVOR) at onset of microgravity, and stage 45 tadpoles, which had already developed the reflex. The main observations were that the developmental progress of the animals was not affected by microgravity; that in the young tadpole group with normal body shape the rVOR was not modified by microgravity, while in the older group with microgravity experience, the rVOR was augmented; and that significant effects on the shape of otoconia and on the number and size of CalBindin-expressing cells of the labyrinthine maculae cells were absent. In addition, behavioural data were never significantly correlated with morphological features of macular structures such as size and number of CalBindin-expressing cells. It is postulated that mechanisms of vestibular adaptation to microgravity during early development are probably based on mechanisms located in central structures of the vestibular system.
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Sensación de Gravedad , Reflejo Vestibuloocular , Vuelo Espacial , Vestíbulo del Laberinto/anatomía & histología , Ingravidez , Xenopus laevis/anatomía & histología , Adaptación Fisiológica , Animales , Calbindinas , Células Ciliadas Vestibulares/química , Larva/anatomía & histología , Larva/fisiología , Membrana Otolítica/fisiología , Membrana Otolítica/ultraestructura , Proteína G de Unión al Calcio S100/análisis , Vestíbulo del Laberinto/química , Vestíbulo del Laberinto/fisiología , Xenopus laevis/fisiologíaRESUMEN
As a result of previous studies using hypergravity (centrifuge) or virtual microgravity (clinostat), it was proposed that gravity was involved in embryonic development, i.e., in the establishment of the embryonic polarities and the body plan pattern which subsequently direct morphogenesis and organogenesis of the central nervous system and of sensory organs. Recent experiments were performed in space using sounding rockets and orbiting space-modules to ascertain whether gravity is indeed required for embryogenesis in Invertebrates and Vertebrates. Eggs fertilised in vivo or in vitro in microgravity showed some abnormalities during embryonic development but were able to regulate and produce nearly normal larvae.
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Anfibios/embriología , Gravitación , Sistema Nervioso/embriología , Animales , Embrión no Mamífero/fisiologíaRESUMEN
BACKGROUND: Numerous products based on soybean are available and various food technologies are applied for their production. The allergenicity of natural soybean may be modified by these treatments. OBJECTIVES: To compare the allergenicity of native soybean proteins with those of soy milk and texturized protein products. To show additional allergens. METHODS: Three commercial products and two infant formulas were studied: Soybean flour, soy milk, texturized soy proteins, two infant formulas; the first containing total proteins and the second containing a soy protein hydrolysate. Sera from 9 patients allergic to soy protein were tested by immunoblotting (IB). IB inhibition was achieved by incubating sera with protein extract from soybean flour. RESULTS: The SDS-PAGE profile of soybean flour protein and soy milk showed a difference in the proportions of the various protein fractions, with a higher concentration of 37-kD protein in flour and 33-kD protein in milk. Infant formula 1 contained proteins with a molecular weight below 28 kD. The texturized extract contained high proportions of 31- to 34- and 38-kD proteins. Immunoblotting revealed a lack of allergenicity in infant formula. Sera recognizing the 38- and 50-kD proteins in texturized soy protein also recognized the 37- and 49-kD proteins in soybean flour and in soy milk, suggesting a protein glycation by texturization processes. The 30- to 34-kD band in texturized proteins was devoid of any allergenicity. This study seems to indicate that the 30-kD allergen (Gly m Bd 30) disappears during the production of texturized soy protein. CONCLUSION: All technologies applied to soybean-based products induce striking variation in the protein profile and allergenicity. Texturized protein could lack the major allergen Gly m Bd 30. Further studies or texturization might generate modified technologies in order to create hypoallergenic texturized proteins.
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Alérgenos/inmunología , Manipulación de Alimentos/métodos , Hipersensibilidad a los Alimentos/inmunología , Tecnología de Alimentos , Glycine max/inmunología , Adolescente , Alérgenos/química , Niño , Preescolar , Proteínas en la Dieta/análisis , Proteínas en la Dieta/inmunología , Electroforesis en Gel de Poliacrilamida , Femenino , Humanos , Immunoblotting , Inmunoglobulina E/sangre , Masculino , Persona de Mediana Edad , Prueba de Radioalergoadsorción , Pruebas Cutáneas , Proteínas de Soja/análisis , Proteínas de Soja/inmunología , Glycine max/químicaRESUMEN
BACKGROUND: Children with severe food allergies can benefit from a personalized care project (PCP) in schools. The usefulness of the PCP and the residual risk of allergic emergencies are poorly appreciated. The objective was to evaluate the efficiency of the management plan and the training in the use of the emergency kit. METHODS: A telephone survey using a detailed questionnaire was performed in 45 families whose children had been previously referred to the department. The distribution of disorders was as follows: asthma, 37.7%; atopic dermatitis and asthma, 28.8%; atopic dermatitis, 15.5%; angioedema and urticaria, 13.3%; and anaphylactic shock, 4.2%. Food allergy had been diagnosed in the 45 children by past history, and double-blind or single-blind, placebo-controlled food challenges (DBPCFCs, or SBPCFCs) with evidence of specific IgE. Exactly 75.5% of the children had peanut allergy. Multiple food allergies characterized 46.8% of the subjects. They had benefited from a strict elimination diet and a protocol for emergency care including a ready-to-use intramuscular epinephrine injection. A PCP had been requested by the School Public Health Service. RESULTS: Thirty-nine PCPs were implemented (86.5% of the requests). They represented 63% of the PCPs for food allergy in the eastern region of France: one per 5800 school-age children. The retrospective period of evaluation was 25 months on average. The types of meals were very diverse, and medically acceptable in 83% of cases. The place where the emergency kit was stored in the school varied. Forty reactions occurred in 33% of the children (5/6 times in the absence of a PCP), asthma in 28%, shock in 1%, and immediate skin reactions in 11%. Reactions occurred at home in 78% of the subjects, and in school in 22% of the subjects. The cause of the reactions was not specifically known in 63% of cases. Twenty-seven percent of the reactions were linked to the ingestion of food allergens. In 10% of subjects, the reaction was due to a modification of ingredients by the food industry. CONCLUSIONS: The frequency of respiratory symptoms during oral challenge tests was confirmed by the frequency of asthmatic reactions within the follow-up period. The role of hidden allergens and of misleading labeling validates the need for PCPs in the case of peanut and tree nut allergies, past history of severe reactions, multiple food allergies, reactions to a low dose in DBPCFCs, and asthmatic reactions to foods. This study provides encouraging data on the usefulness of PCPs and confirms the need for thorough instruction and training of the school staff in dealing with allergic emergencies. Addition of a beta-agonist spray to the emergency kit is suggested.
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Anafilaxia/etiología , Hipersensibilidad a los Alimentos/complicaciones , Servicios de Salud Escolar , Adolescente , Agonistas Adrenérgicos beta/uso terapéutico , Adulto , Anafilaxia/terapia , Angioedema/etiología , Especificidad de Anticuerpos/inmunología , Arachis/efectos adversos , Asma/etiología , Niño , Protección a la Infancia , Preescolar , Dermatitis Atópica/etiología , Método Doble Ciego , Huevos/efectos adversos , Epinefrina/uso terapéutico , Femenino , Estudios de Seguimiento , Hipersensibilidad a los Alimentos/inmunología , Hipersensibilidad a los Alimentos/terapia , Francia/epidemiología , Humanos , Inmunoglobulina E/inmunología , Masculino , Valor Predictivo de las Pruebas , Prueba de Radioalergoadsorción , Método Simple Ciego , Pruebas Cutáneas , Encuestas y Cuestionarios , Resultado del TratamientoRESUMEN
The molecular mechanism of neural induction is still unknown and the identity of the natural inducer remains elusive. It has been suggested that both the protein kinase C and cAMP signal transduction pathways may be involved in mediating its action. Here we provide evidence that Ca2+ is implicated in the process of transduction of the neuralizing signal. We find that an increase in intracellular Ca2+ concentration [Ca2+]i occurs during neural induction provoked in vitro by the lectin Con A in Pleurodeles waltl embryo. We demonstrate that specific L-type Ca2+ channel agonists also trigger neural induction. Conversely, noninducing lectins do not raise [Ca2+]i. Ryanodine and caffeine trigger neural induction. An increase in [Ca2+]i was also observed after treatment with the phorbol 12-myristate 13-acetate, which has been reported to be inductive. The [Ca2+]i increase triggered by phorbol ester and Con A was abolished by staurosporine and by L-type Ca2+ channel antagonists. Our findings demonstrate that the [Ca2+]i increase occurs via L-type Ca2+ channels. We suggest an amplification of this increase by a Ca(2+)-induced Ca2+ release mechanism which involves intracellular ryanodine-sensitive stores. We propose that Ca(2+)-dependent processes controlled by protein kinase C are implicated in the regulation of gene expression in response to neural induction.
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Canales de Calcio/fisiología , Calcio/fisiología , Sistema Nervioso/embriología , Alcaloides/farmacología , Animales , Cafeína/farmacología , Células Cultivadas , Concanavalina A/farmacología , Ectodermo/fisiología , Inducción Embrionaria , Técnicas In Vitro , Activación del Canal Iónico/efectos de los fármacos , Isradipino/farmacología , Nimodipina/farmacología , Pleurodeles , Proteína Quinasa C/fisiología , Rianodina/farmacología , Estaurosporina , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
The technique of electropulsation has been shown to be highly efficient in promoting penetration of exogenous molecules into living cells, transfection, and cell fusion in different animal, vegetal, and bacterial cell systems. Introduction of such exogenous compounds, i.e., plasmids, into living cells is of great interest for embryological studies. Embryonic amphibian ectodermal cells from Pleurodeles waltl gastrulae, either freshly dissociated or cultured for 5 days, can be permeabilized when submitted to an external electric field of sufficient intensity: 500 V/cm for isolated spherical cells and 150-200 V/cm for plated cultured cells. Permeabilization was indicated by both the leakage of metabolites (ATP) from the cells and the uptake of exogenous compounds (pyranin) into the cells. With the use of higher field intensities (600 V/cm for freshly dissociated cells and 300 V/cm for cultured cells) cell fusion and syncytial structures could also be obtained. Isolated spherical cells had 100% viability immediately after being pulsed at intensities up to 600 V/cm. Cell lysis was observed above this value, although the nonlysed cells were observed to spread on a substrate and differentiate normally. For the cultured plated cells, cell viability fell with increasing electric-field strength, and for a given electric field value, cell viability decreased with the age of the culture after pulsing. Nevertheless, for electric-field intensities less than or equal to 300 V/cm, 100% of the cells remained attached to the substrate and differentiated normally over the following 5 days.
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Fusión Celular , Permeabilidad de la Membrana Celular , Ectodermo/citología , Pleurodeles/embriología , Salamandridae/embriología , Adenosina Trifosfato/metabolismo , Animales , Supervivencia Celular , Células Cultivadas , Electricidad , Colorantes Fluorescentes , Microscopía FluorescenteRESUMEN
The FERTILE experiment was twice performed onboard the Mir space station during the Cassiopée and Pégase French space missions. The goal was to analyze the effects of microgravity on fertilization and embryonic development, and then on further development on the ground in the amphibian Pleurodeles waltl. The present paper reports development that occurred in the laboratory after landing. Recovered on the ground at the hatching stage, young larvae reared at room temperature underwent metamorphosis and became adults without obvious abnormalities. Of particular interest was the rearing temperature that induced a delayed metamorphosis for animals from the Cassiopée space mission, but not for animals from the Pégase mission. The rate of development and the morphology were analogous in these animals and in ground controls reared in a similar annual period. Analysis of offspring was performed using these animals. Males born in space were first mated with control ground-born females and then with females born in space. The mating gave progeny that developed normally. Depending on the methods used and on the limits of the analyses, the results clearly demonstrated that animals born in space were able to live and reproduce after return to the ground.
Asunto(s)
Pleurodeles/fisiología , Vuelo Espacial , Ingravidez , Animales , Embrión no Mamífero/fisiología , Desarrollo Embrionario , Femenino , Larva/crecimiento & desarrollo , Larva/fisiología , Masculino , Metamorfosis Biológica , Péptido Hidrolasas/metabolismo , Pleurodeles/embriología , Pleurodeles/crecimiento & desarrollo , TemperaturaRESUMEN
Effects of microgravity (microG) on fertilization were studied in the urodele amphibian Pleurodeles waltl on board the MIR space station. Genetic and cytomorphologic analyses ruled out parthenogenesis or gynogenesis and proved that fertilization did occur in microG. Actual fertilization was demonstrated by the analysis of the distribution of peptidase-1 genes, a polymorphic sex-linked enzyme, in progenies obtained in microG. Further evidence of fertilization was provided by the presence of spermatozoa in the perivitelline space and in the fertilization layer of the microG eggs and by the presence of a female pronucleus and male pronuclei in the egg cytoplasm. Experiments in microG and in 1.4G, 2G, and 3G hypergravity showed for the first time that, compared to eggs in 1G, several characteristics of the fertilization process including the cortical reaction and the microvillus transformations were altered depending on the gravitational force applied to the eggs. Microvillus elevation, the most evident feature, was reduced on microG-eggs and amplified on eggs submitted to 2G and 3G. No lethal consequences of these alterations on the early development of microG-eggs were observed.