RESUMEN
Peripheral blood C-reactive protein (CRP) is a biomarker used clinically to measure systemic inflammation and is reproducibly increased in a subset of patients with major depressive disorder (MDD). Furthermore, increased peripheral blood CRP in MDD has been associated with altered reward circuitry and increased brain glutamate in relation with symptoms of anhedonia. Nevertheless, the relationship between peripheral CRP and other peripheral and central markers of inflammation in depressed patients has not been established. Plasma (n = 89) and CSF (n = 73) was collected from medically stable, currently unmedicated adult outpatients with MDD. Associations among plasma and CSF CRP and plasma and CSF inflammatory cytokines (interleukin [IL]-6, tumor necrosis factor [TNF] and IL-1beta) and their soluble receptors/antagonists were examined. Relationships between plasma and CSF inflammatory markers and depressive symptoms including anhedonia and reduced motivation (RM) were also explored. Plasma CRP was correlated with multiple plasma inflammatory markers (all p < 0.05), and a strong correlation was found between plasma and CSF CRP (r = 0.855, p < 0.001). CSF CRP in turn correlated with CSF cytokine receptors/antagonists (all p < 0.05). Principal component analyses revealed clusters of CSF inflammatory markers that were associated with high plasma CRP (>3 mg/L) and correlated with depressive symptom severity. These findings were driven by CSF TNF, which correlated with RM (r = 0.236, p = 0.045), and CSF IL-6 soluble receptor, which correlated with anhedonia (r = 0.301, p = 0.010) in the sample as a whole and particularly females. CRP appears to be a peripheral biomarker that reflects peripheral and central inflammation and seems well-suited for guiding immunotherapies targeting TNF and IL-6 in patients with MDD.
Asunto(s)
Proteína C-Reactiva/análisis , Trastorno Depresivo Mayor/sangre , Inflamación/sangre , Adulto , Anciano , Citocinas/sangre , Citocinas/inmunología , Depresión/sangre , Femenino , Humanos , Masculino , Persona de Mediana Edad , Plasma/química , Plasma/inmunología , Adulto JovenRESUMEN
Pericarditis is a source of chest pain that can be chronic and debilitating. We describe a patient diagnosed with pericarditis with chest pain refractory to medical management. This pain was significantly relieved by a left stellate ganglion block, and a subsequent thoracic chain sympathectomy resulted in 3 months of gradually diminishing relief. Possible reasons for this result may include incomplete transection, a significant contribution of pain signaling from the phrenic and/or vagus nerve, or nerve regeneration. Literature describing these interventional techniques for pericardial pain is sparse, and more research is needed to determine their efficacy in refractory pericardial pain.
Asunto(s)
Dolor en el Pecho/terapia , Dolor Intratable/terapia , Simpatectomía , Procedimientos Quirúrgicos Torácicos , Bloqueo Nervioso Autónomo , Femenino , Humanos , Persona de Mediana Edad , Ganglio EstrelladoAsunto(s)
Analgésicos Opioides/farmacología , Inhibidores Selectivos de la Recaptación de Serotonina/farmacología , Síndrome de la Serotonina/inducido químicamente , Antidepresivos Tricíclicos/farmacología , Celecoxib , Dolor Crónico/complicaciones , Dolor Crónico/tratamiento farmacológico , Inhibidores de la Ciclooxigenasa 2/farmacología , Trastorno Depresivo/complicaciones , Trastorno Depresivo/tratamiento farmacológico , Desipramina/farmacología , Interacciones Farmacológicas , Clorhidrato de Duloxetina , Fentanilo/administración & dosificación , Fentanilo/farmacología , Humanos , Masculino , Metadona/farmacología , Persona de Mediana Edad , Oxicodona/farmacología , Polifarmacia , Pirazoles/farmacología , Sulfonamidas/farmacología , Tiofenos/farmacología , Parche TransdérmicoRESUMEN
BACKGROUND: Loss of the cell membrane protein E-cadherin is a critical event during Ras-mediated transformation of intestinal epithelial cells. The purpose of our study is to determine if activation of the transcriptional repressor SLUG is an important component of the mechanism of Ras-induced loss of E-cadherin. METHODS: Rat intestinal epithelial (RIE) cells were engineered to express mutated human Ha-Ras(Val12) complementary DNA (H-Ras cells). Cell morphology was examined by light microscopy. RNA and protein expression were measured by semiquantitative polymerase chain reaction and Western blot analyses, respectively. Short interfering RNA with 2 different oligos was used to knock down the expression of SLUG. RESULTS: Oncogenic ras induces upregulation of the transcriptional repressor SLUG and subsequent downregulation of the junctional protein E-cadherin. Gene silencing of SLUG by short interfering RNA allows E-cadherin to be reexpressed. E-cadherin protein reexpression allows partial rescue of the transformed phenotype. CONCLUSION: These data suggest a mechanism whereby Ras signaling causes an upregulation of transcriptional repressors and subsequent downregulation of E-cadherin as a malignant phenotype is propagated.