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Mol Biol Rep ; 39(12): 11023-30, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23053992

RESUMEN

The first complete mitochondrial genome (mitogenome) of Tachinidae Exorista sorbillans (Diptera) is sequenced by PCR-based approach. The circular mitogenome is 14,960 bp long and has the representative mitochondrial gene (mt gene) organization and order of Diptera. All protein-coding sequences are initiated with ATN codon; however, the only exception is Cox I gene, which has a 4-bp ATCG putative start codon. Ten of the thirteen protein-coding genes have a complete termination codon (TAA), but the rest are seated on the H strand with incomplete codons. The mitogenome of E. sorbillans is biased toward A+T content at 78.4 %, and the strand-specific bias is in reflection of the third codon positions of mt genes, and their T/C ratios as strand indictor are higher on the H strand more than those on the L strand pointing at any strain of seven Diptera flies. The length of the A+T-rich region of E. sorbillans is 106 bp, including a tandem triple copies of a13-bp fragment. Compared to Haematobia irritans, E. sorbillans holds distant relationship with Drosophila. Phylogenetic topologies based on the amino acid sequences, supporting that E. sorbillans (Tachinidae) is clustered with strains of Calliphoridae and Oestridae, and superfamily Oestroidea are polyphyletic groups with Muscidae in a clade.


Asunto(s)
Dípteros/genética , Evolución Molecular , Genoma Mitocondrial/genética , Animales , Composición de Base/genética , Secuencia de Bases , ADN Intergénico/genética , Drosophila/genética , Genes Mitocondriales/genética , Genes de ARNr/genética , Variación Genética , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Filogenia , ARN de Transferencia/genética , Análisis de Secuencia de ADN , Factores de Tiempo
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