RESUMEN
The bactericidal properties of normal serum are an important feature in host defence; it has been suggested that they are depressed by cigarette smoke. The aim of the present study was to investigate the in vitro depressant effect of cigarette smoke on antibacterial activity of rabbit serum and its interaction with the well-known antioxidant agent, N-acetylcysteine. Escherichia coli was used to study the bactericidal activity of the complement-dependent system and Staphylococcus aureus was used to study the thermostable bactericidal system. It was found that exposure of serum and bacteria to cigarette smoke significantly decreased the bactericidal powder of serum; when N-acetylcysteine was added to the incubation mixture, however, a marked inhibitory effect on the toxic action of smoke on rabbit serum was observed.
Asunto(s)
Acetilcisteína/farmacología , Escherichia coli/inmunología , Humo/efectos adversos , Animales , Sangre/efectos de los fármacos , Supervivencia Celular , Escherichia coli/efectos de los fármacos , Plantas Tóxicas , Conejos , Staphylococcus aureus/efectos de los fármacos , NicotianaRESUMEN
The synthesis of 3-bromo-4,5,6,7-tetrahydro-1,2-benzoisoxazole derivatives and their activity against human pathogen fungi are reported. In particular, compound 3,5-dibromo-6,7-dihydro-1,2-benzoisoxazol-4-(5H)-one showed a broad antifungal spectrum and good fungicidal activity against Trichophyton mentagrophytes.
Asunto(s)
Antifúngicos/síntesis química , Isoxazoles/síntesis química , Animales , Antifúngicos/farmacología , Antifúngicos/toxicidad , Hongos/efectos de los fármacos , Técnicas In Vitro , Isoxazoles/farmacología , Isoxazoles/toxicidad , Pruebas de Sensibilidad Microbiana , Pruebas de Mutagenicidad , Mutágenos , Ratas , Salmonella typhimurium/efectos de los fármacosRESUMEN
The present study was undertaken to define an interpretative guideline for disk diffusion susceptibility testing with fosfomycin trometamol, a new antimicrobial agent which has been developed for the treatment of urinary tract infections. Two potencies of fosfomycin disk were used: 50 and 200 micrograms, prepared in the presence and absence of glucose-6-phosphate. To verify the reliability of the results obtained in susceptibility testing, we have also evaluated the bactericidal activity of fosfomycin trometamol versus sensitive and resistant strains in an 'in vitro' model simulating the hydrokinetic aspects involved in the treatment of bacterial cystitis. The data obtained evidenced the role of glucose-6-phosphate in antimicrobial susceptibility tests as well as the importance of the urinary antibiotic concentrations to define sensitive and resistant bacteria. On the basis of our results, we recommend that a 200-microgram disk of fosfomycin containing 50 micrograms of glucose-6-phosphate should be used in antimicrobial susceptibility testing with fosfomycin trometamol.