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J Invest Surg ; 13(6): 333-41, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-11202010

RESUMEN

Synthetic materials currently used for arterial grafts can provoke serious complications such as infection, rejection, and incomplete reendothelialization. Porcine small intestinal submucosa (SIS) allografts (homografts) may not share these disadvantages yet still may provide an effective graft alternative. This study was designed to provide additional information concerning the performance of this material when used as an allo-patch graft. A porcine model was selected to enable hemodynamic comparisons to the adult human physiology. Slaughterhouse small intestine was used to prepare the SIS graft material. A well-vascularized section ofjejunum was resected, rinsed, and maintained in 0.9% NaCl solution containing 10% gentamicin. The graft was prepared by removing the serosal layer, inverting the material, and discarding the mucosal surface in a similar manner. The remaining submucosa and stratum compactum constituted the graft material and was subdivided into sizes from 1.5 to 3.0 cm2 tacked to foil for manageability, and was returned to the gentamicin solution. The surgery was initiated with a femoral arterial line in the anesthetized swine, followed by isolation and preparation of the infrarenal aorta for grafting. Cross-clamps were applied and a 1.0 x 1.5-cm section of aortic wall was resected. Two sides of allograft were freed from the foil and sewn into the aorta. Then the remaining sides were removed from the foil and final graft placement was completed. The graft was harvested at postoperative day 28 and presented for microscopic examination. Results confirmed that reendothelialization with neovascularization had occurred during this interval.


Asunto(s)
Aorta/cirugía , Prótesis Vascular , Mucosa Intestinal/trasplante , Yeyuno/trasplante , Animales , Bicarbonatos/sangre , Capilares , Frecuencia Cardíaca , Mucosa Intestinal/irrigación sanguínea , Yeyuno/irrigación sanguínea , Proyectos Piloto , Porcinos , Trasplante Homólogo , Procedimientos Quirúrgicos Vasculares/métodos
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