RESUMEN
KEY MESSAGE: The rust resistance genes Lr53 and Yr35 were introgressed into bread wheat from Aegilops longissima or Aegilops sharonensis or their S-genome containing species and mapped to the telomeric region of chromosome arm 6BS. Wheat leaf and stripe rusts are damaging fungal diseases of wheat worldwide. Breeding for resistance is a sustainable approach to control these two foliar diseases. In this study, we used SNP analysis, sequence comparisons, and cytogenetic assays to determine that the chromosomal segment carrying Lr53 and Yr35 was originated from Ae.longissima or Ae. sharonensis or their derived species. In seedling tests, Lr53 conferred strong resistance against all five Chinese Pt races tested, and Yr35 showed effectiveness against Pst race CYR34 but susceptibility to race CYR32. Using a large population (3892 recombinant gametes) derived from plants homozygous for the ph1b mutation obtained from the cross 98M71 × CSph1b, both Lr53 and Yr35 were successfully mapped to a 6.03-Mb telomeric region of chromosome arm 6BS in the Chinese Spring reference genome v1.1. Co-segregation between Lr53 and Yr35 was observed within this large mapping population. Within the candidate region, several nucleotide-binding leucine-rich repeat genes and protein kinases were identified as candidate genes. Marker pku6B3127 was completely linked to both genes and accurately predicted the absence or presence of alien segment harboring Lr53 and Yr35 in 87 tetraploid and 149 hexaploid wheat genotypes tested. We developed a line with a smaller alien segment (< 6.03 Mb) to reduce any potential linkage drag and demonstrated that it conferred resistance levels similar to those of the original donor parent 98M71. The newly developed introgression line and closely linked PCR markers will accelerate the deployment of Lr53 and Yr35 in wheat breeding programs.
Asunto(s)
Aegilops , Mapeo Cromosómico , Resistencia a la Enfermedad , Genes de Plantas , Puccinia , Aegilops/genética , Aegilops/microbiología , Cromosomas de las Plantas/genética , Resistencia a la Enfermedad/genética , Introgresión Genética , Ligamiento Genético , Marcadores Genéticos , Fitomejoramiento , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Polimorfismo de Nucleótido Simple , Puccinia/fisiología , Triticum/genética , Triticum/microbiologíaRESUMEN
Industrialization and urbanization have posed serious threats to the environment. Excessive release of heavy metals from industrial effluents and overuse of pesticides in modern agriculture are limiting crop production by polluting environment and deteriorating food quality. Sustaining food quality under heavy metals and pesticide stress is crucial to meet the increasing demands for food. 24-Epibrassinolide (EBL), a ubiquitously occurring plant growth hormone shows great potential to alleviate heavy metals and pesticide stress in plants. This review sums up the potential role of EBL in ameliorating heavy metals and pesticide toxicity in plants extensively. EBL application increases plant's overall growth, biomass accumulation and photosynthetic efficiency by the modulation of numerous biochemical and physiological processes under heavy metals and pesticide stress. In addition, EBL scavenges reactive oxygen species (ROS) by triggering the production of antioxidant enzymes such as SOD, CAT, POX etc. EBL also induces the production of proline and soluble proteins that helps in maintaining osmotic potential and osmo-protection under both heavy metals and pesticide stress. At the end, future needs of research about the application of 24-epibrassinolide have also been discussed.
Asunto(s)
Brasinoesteroides/farmacología , Productos Agrícolas/efectos de los fármacos , Metales Pesados/toxicidad , Estrés Oxidativo/efectos de los fármacos , Plaguicidas/toxicidad , Reguladores del Crecimiento de las Plantas/farmacología , Contaminantes del Suelo/toxicidad , Esteroides Heterocíclicos/farmacología , Antioxidantes/metabolismo , Productos Agrícolas/metabolismo , Especies Reactivas de Oxígeno/metabolismoRESUMEN
The early detection of breast cancer using mammogram images is critical for lowering women's mortality rates and allowing for proper treatment. Deep learning techniques are commonly used for feature extraction and have demonstrated significant performance in the literature. However, these features do not perform well in several cases due to redundant and irrelevant information. We created a new framework for diagnosing breast cancer using entropy-controlled deep learning and flower pollination optimization from the mammogram images. In the proposed framework, a filter fusion-based method for contrast enhancement is developed. The pre-trained ResNet-50 model is then improved and trained using transfer learning on both the original and enhanced datasets. Deep features are extracted and combined into a single vector in the following phase using a serial technique known as serial mid-value features. The top features are then classified using neural networks and machine learning classifiers in the following stage. To accomplish this, a technique for flower pollination optimization with entropy control has been developed. The exercise used three publicly available datasets: CBIS-DDSM, INbreast, and MIAS. On these selected datasets, the proposed framework achieved 93.8, 99.5, and 99.8% accuracy, respectively. Compared to the current methods, the increase in accuracy and decrease in computational time are explained.
RESUMEN
Polychlorinated biphenyls (PCBs) are synthetic organic compounds ubiquitously distributed worldwide due to their persistence, long-range atmospheric transport, and bioaccumulation. Owing to teratogenic properties, PCBs are a global environmental problem. Different physical, biological, and chemical techniques are utilized for the remediation of PCBs. This review paper discusses the recent development in photocatalytic and chemical techniques for the remediation of PCBs in contaminated soils. In particular, the photocatalytic degradation of PCBs combined with soil washing, Fe-based reductive dichlorination, and advanced oxidation process (Fenton advance oxidation and persulfate oxidation) is discussed and reviewed in detail. The review suggested that advanced oxidation is an efficient remediation technique with 77-99% of removal efficiency of PCBs. Persulfate oxidation is the most suitable technique which could work at normal environmental conditions (such as pH, temperature, soil organic matter (SOM), etc.). Different environmental factors such as pH, temperature, and SOM affect the Fe-based reductive dechlorination and Fenton advance oxidation techniques. The surfactants and organic solvents used in soil washing combined with photocatalytic degradation affect the degradation capability of these techniques. This review will contribute to PCBs degradation by the detailed discussion of development in chemical technique future perspective and research needs.