Understanding the influence of the bioaerosol source on the distribution of airborne bacteria in hospital indoor air.

The composition and concentration of airborne microorganisms in hospital indoor air has been reported to contain airborne bacteria and fungi concentrations ranged 101-103 CFU/m3 in inpatients facilities which mostly exceed recommendations from the World Health Organization (WHO). In this work, a deeper knowledge of the performance of airborne microorganisms would allow improving the designs of the air-conditioning installations to restrict hospital-acquired infections (HAIs). A solution containing Escherichia coli (E. coli) as a model of airborne bacteria was nebulized using the Collison nebulizer to simulate bioaerosols in various hospital areas such as patients' rooms or bathrooms. Results showed that the bioaerosol source had a significant influence on the airborne bacteria concentrations since 4.00 102, 6.84 103 and 1.39 104 CFU mL-1 were monitored during the aerosolization for 10 min of urine, saliva and urban wastewater, respectively. These results may be explained considering the quite narrow distribution profile of drop sizes around 1.10-1.29 µm obtained for urban wastewater, with much vaster distribution profiles during the aerosolization of urine or saliva. The airborne bacteria concentration may increase up to 107 CFU mL-1 for longer sampling times and higher aerosolization pressures, causing several cell damages. The cell membrane damage index (ID) can vary from 0 to 1, depending on the genomic DNA releases from bacteria. In fact, the ID of E. coli was more than two times higher (0.33 vs. 0.72) when increasing the pressure of air flow was applied from 1 to 2 bar. Finally, the ventilation air flow also affected the distribution of bioaerosols due to its direct relationship with the relative humidity of indoor air. Specifically, the airborne bacteria concentration diminished almost below 3-logs by applying more than 10 L min-1 during the aerosolization of urine due to their inactivation by an increase in their osmotic pressure.

Cleaning technologies integrated in duct flows for the inactivation of pathogenic microorganisms in indoor environments: A critical review of recent innovations and future challenges.

Pathogenic microorganisms are a major concern in indoor environments, particularly in sensitive facilities such as hospitals, due to their potential to cause nosocomial infections. This study evaluates the concentration of airborne bacteria and fungi in the University Hospital Complex of Albacete (Spain), comparing the results with recent literature. Staphylococcus is identified as the most prevalent bacterial genus with a percentage distribution of 35%, while Aspergillus represents the dominant fungal genus at 34%. The lack of high Technology Readiness Levels (TRL 6, TRL 7) for effective indoor air purification requires research efforts to bridge this knowledge gap. A screening of disinfection technologies for pathogenic airborne microorganisms such as bacteria and fungi is conducted. The integration of filtration, irradiation or and (electro)chemical gas treatment systems in duct flows is discussed to enhance the design of the air-conditioning systems for indoor air purification. Concerns over microbial growth have led to recent studies on coating commercial fibrous air filters with antimicrobial particles (silver nanoparticles, iron oxide nanowires) and polymeric materials (polyaniline, polyvinylidene fluoride). Promising alternatives to traditional short-wave UV-C energy for disinfection include LED and Far-UVC irradiation systems. Additionally, research explores the use of TiO2 and TiO2 doped with metals (Ag, Cu, Pt) in filters with photocatalytic properties, enabling the utilization of visible or solar light. Hybrid photocatalysis, combining TiO2 with polymers, carbon nanomaterials, or MXene nanomaterials, enhances the photocatalytic process. Chemical treatment systems such as aerosolization of biocidal agents (benzalkonium chloride, hydrogen peroxide, chlorine dioxide or ozone) with their possible combination with other technologies such as adsorption, filtration or photocatalysis, are also tested for gas disinfection. However, the limited number of studies on the use of electrochemical technology poses a challenge for further investigation into gas-phase oxidant generation, without the formation of harmful by-products, to raise its TRL for effectively inactivating airborne microorganisms in indoor environments.

Biostimulation versus bioaugmentation for the electro-bioremediation of 2,4-dichlorophenoxyacetic acid polluted soils.

The aim of this work is to compare three biological strategies for the in situ remediation of a 2,4-dichlorophenoxyacetic acid (2,4-D) polluted clayey soil by coupling electrokinetics (EK) and bioremediation (technology named as electrobioremediation, EBR). The first option (i) is EK-biostimulation, in which the activity of microorganisms already present in soil is enhanced by EK phenomena. The second and third options are EK-bioaugmentation, which consist of addition of microorganisms to soil through the inclusion of permeable biological barriers: (ii) using a microbial fixed biofilm reactor as biobarrier (BB1), and (iii) using a mixture of clean soil and a microbial suspension as biobarrier (BB2). Thus, three batch experiments at bench scale were conducted under a constant electric field of 1 V cm-1, and electrode polarity was periodically reversed every 12 h (2 d-1). The duration of each test was 10 days. Two additional tests using only biodegradation or only EK were performed as auxiliary reference tests. A microbial consortium acclimated to 2,4-D biodegradation was employed. Results showed that EK-biostimulation strategy offered the best pollutant removal efficiency (reaching up almost 100%) while biobarriers offered pollutant removal rates between 75 and 85%. Permeable biobarriers allowed the introduction of microorganism but caused a decrease in the electro-osmotic flow which, in turn, reduced the mobilization and contact between microorganisms and pollutants. These results can contribute to the knowledge and understanding of electrobioremediation of polluted soil and to the feasibility of delivering microorganism to the soil by using biobarriers. Despite biostimulation was found to be the best option, results show that permeable reactive biobarriers may result in a successful alternative for in-situ EK-bioaugmentation when acclimated microbial population is not already present in soil.

Electrochemical Technologies to Decrease the Chemical Risk of Hospital Wastewater and Urine.

The inefficiency of conventional biological processes to remove pharmaceutical compounds (PhCs) in wastewater is leading to their accumulation in aquatic environments. These compounds are characterized by high toxicity, high antibiotic activity and low biodegradability, and their presence is causing serious environmental risks. Because much of the PhCs consumed by humans are excreted in the urine, hospital effluents have been considered one of the main routes of entry of PhCs into the environment. In this work, a critical review of the technologies employed for the removal of PhCs in hospital wastewater was carried out. This review provides an overview of the current state of the developed technologies for decreasing the chemical risks associated with the presence of PhCs in hospital wastewater or urine in the last years, including conventional treatments (filtration, adsorption, or biological processes), advanced oxidation processes (AOPs) and electrochemical advanced oxidation processes (EAOPs).

Biodegradability improvement of clopyralid wastes through electrolysis using different diamond anodes.

The use of boron-doped (BDDs) anodes for efficient removal of complex organic molecules, such as organochlorine compounds, is well stated in the literature. However, the role of the different characteristics of this anode on the transformation of these type of contaminants into more biodegradable molecules is a topic of interest that need to be clarified when aimed an efficient combination of an electrochemical system as a previous step to biological treatment. In this work, improvement in the biodegradability of synthetic wastes polluted with clopyralid, as an organochlorine model compound, is studied after electrolysis with different BDDs in the presence of the two most common supporting electrolytes (containing sulfate or chloride ions). For that, clopyralid removal, mineralization, aromatics intermediates, short-chain carboxylic acids, and inorganic ions were monitored. Improved results were found in sulfate media for BDD with 200 ppm, capable of removing 88.7% of contaminants and 85% of TOC, resulting in an improvement in biodegradability of almost 7-fold compared to the initial sample. These findings point out that lower doping levels are preferable when coupling studied technologies.

Single and Coupled Electrochemical Processes and Reactors for the Abatement of Organic Water Pollutants: A Critical Review.

Traditional physicochemical and biological techniques, as well as advanced oxidation processes (AOPs), are often inadequate, ineffective, or expensive for industrial water reclamation. Within this context, the electrochemical technologies have found a niche where they can become dominant in the near future, especially for the abatement of biorefractory substances. In this critical review, some of the most promising electrochemical tools for the treatment of wastewater contaminated by organic pollutants are discussed in detail with the following goals: (1) to present the fundamental aspects of the selected processes; (2) to discuss the effect of both the main operating parameters and the reactor design on their performance; (3) to critically evaluate their advantages and disadvantages; and (4) to forecast the prospect of their utilization on an applicable scale by identifying the key points to be further investigated. The review is focused on the direct electrochemical oxidation, the indirect electrochemical oxidation mediated by electrogenerated active chlorine, and the coupling between anodic and cathodic processes. The last part of the review is devoted to the critical assessment of the reactors that can be used to put these technologies into practice.

New perspectives for Advanced Oxidation Processes.

Advanced Oxidation Processes (AOPs) are called to fill the gap between the treatability attained by conventional physico-chemical and biological treatments and the day-to-day more exigent limits fixed by environmental regulations. They are particularly important for the removal of anthropogenic pollutants and for this reason, they have been widely investigated in the last decades and even applied in the treatment of many industrial wastewater flows. However, despite the great development reached, AOPs cannot be considered mature yet and there are many new fields worthy of research. Some of them are going to be briefly introduced in this paper, including hybrid processes, heterogeneous semiconductor photocatalysis, sulphate-radical oxidation and electrochemical advanced oxidation for water/wastewater treatment. Moreover, the use of photoelectrochemical processes for energy production is discussed. The work ends with some perspectives that can be of interest for the ongoing and future research.

Geotechnical behaviour of low-permeability soils in surfactant-enhanced electrokinetic remediation.

Electrokinetic processes provide the basis of a range of very interesting techniques for the remediation of polluted soils. These techniques consist of the application of a current field in the soil that develops different transport mechanisms capable of mobilizing several types of pollutants. However, the use of these techniques could generate nondesirable effects related to the geomechanical behavior of the soil, reducing the effectiveness of the processes. In the case of the remediation of polluted soils with plasticity index higher than 35, an excessive shrinkage can be observed in remediation test. For this reason, the continued evaporation that takes place in the sample top can lead to the development of cracks, distorting the electrokinetic transport regime, and consequently, the development of the operation. On the other hand, when analyzing silty soils, in the surroundings of injection surfactant wells, high seepages can be generated that give rise to the development of piping processes. In this article methods are described to allow a reduction, or to even eliminate, both problems.

Design, Validation, and Fabrication of a Tailored Electrochemical Reactor Using 3D Printing for Studies of Commercial Boron-Doped Diamond Electrodes.

Boron-doped diamond (BDD) electrodes are the most effective and resistant electrodic materials to perform advanced oxidation processes. Having a reactor that can provide adequate hydrodynamic conditions is mandatory to use these electrodes effectively. In this work, the diamond anode electrochemical reactor (E3L-DAER) is designed to fulfill this necessity. Several features are included to improve its efficiency, like conic inlet/outlet, flow enhancers, and a reduced interelectrode gap. The fluid dynamic validation has been performed using computer fluid dynamics (CFD) calculations, residence time distribution (RDT) curves, and mass transfer analysis. The reactor has been made using a three-dimensional (3D) printing stereolithography (SLA) technique, which allows us to build chemical-resistant reactors with nonstandard and tailored features in a cheap and fast way. The obtained results demonstrate that the designed reactor has the required fluid dynamics properties to perform reliable BDD electrode studies and applications. Finally, a BDD electrode was used to test the production of different oxidants such as persulfate, peroxophosphate, and chlorine-derived species.

Treatment of Organics in Wastewater Using Electrogenerated Gaseous Oxidants.

This work focuses on the comparison of the performance of direct electrochemical oxidation with indirect electrolysis mediated by gaseous oxidants in the treatment of diluted wastewater. To do this, energy consumptions of the electrolysis using mixed metal oxide (MMO) electrodes are compared with those required for the production and use of chlorine dioxide in the degradation of methomyl contained in aqueous solutions. Results demonstrate the feasibility of the mediated oxidation process and that this process is competitive with direct oxidation. The oxidants are produced under optimized conditions using the same anodic material applied for the direct degradation of organics, thus avoiding efficiency losses associated with mass transfer limitations in the degradation of dilute organic solutions. Thus, using the ClO2 gaseous oxidant, a concentration of 0.1 mM of methomyl from a solution containing 500 mL is completely removed with an energy consumption as low as 50 Wh. The application of the same energy to a direct electrolytic process for treating the same wastewater can only reach less than half of this removal. These findings may have a very important application in the use of electrochemical technology to achieve the remediation of persistent pollutants in wastewater, where their low concentrations typically make direct processes very inefficient.

Remediation of soils contaminated with methomyl using electrochemically produced gaseous oxidants.

This prospective work focuses on the use of two different gaseous oxidants (chlorine dioxide and ozone) to remediate soil polluted with methomyl in two different applications: ex-situ and in-situ. In the first, the soil washing is integrated with the bubbling of the oxidant, while in the second, the gas was introduced by a perforated pipe located sub-superficially. Regarding the soil washing treatment, results demonstrate that direct use of ozone is not very efficient, although an important improvement is obtained following activation with hydrogen peroxide or UV light. In contrast, chlorine dioxide exhibited complete methomyl depletion from the soil, although with higher energy consumption and technical complexity compared to ozone. The direct dosing of the gaseous oxidants in perforated pipes is effective, achieving methomyl removals of 7.8 % and 9.2 % using ozone and chlorine dioxide, respectively. In these cases, soil conditions are not significantly modified, which becomes an important advantage of the technology as compared with other electrochemically assisted soil remediation process, in which large regions of the treated soil are affected by important changes in the pH or by depletion of ions. This lower impact makes these novel technologies more promising for further evaluations.

New MMO coatings for electro-refinery applications: Promoting the production of carboxylates.

Within the new circular economy paradigm, this work evaluates the performance of tailored mixed metal oxides (MMO) anodes, based on ruthenium and antimony, for their application into an electrochemically-assisted organic refinery process. This process is designed to transform pollutants into value-added products with minimal mineralization. Oxidation of synthetic wastes consisting of phenol solutions was used to validate the electrochemical conversion of phenolic wastes into carboxylates, which are then considered as bricks to be used for electrosynthesis or to produce fuels. The MMO anodes were manufactured using two synthesis routes (Pechini method and ionic liquid method), each followed by one of three different heating treatments: furnace, microwave, and CO2 laser. The selection of the optimal electrode for the organic electrorefinery was based on a combination of physical and electrochemical properties, degradation performance of phenol to carboxylates, and long-term stability, looking for a truly sustainable solution. Results indicate that anodes synthesized by the ionic liquid (IL) method, regardless of the heating treatment, demonstrated superior performance, with larger active areas (with furnace 82 mC cm-2, microwave 97 mC cm-2, and laser 127 mC cm-2) and higher phenol degradation rates, resulting in a greater generation of carboxylates during electrolysis, yielding primarily oxalate and achieving up to 40% conversion with furnace heating. However, laser-treated anodes exhibited greater stability than furnace-made ones, attributed to the formation of an insulating TiO2 layer. Although the electrode with the longest service life did not show the best catalytic properties for minimizing mineralization, the observed variations in coatings with identical chemical compositions highlight the importance of this research. This study positions itself at the forefront of developing more efficient and sustainable electrochemical technologies for organic waste treatment.

Improved Operation of Chloralkaline Reversible Cells with Mixed Metal Oxide Electrodes Made Using Microwaves.

This study focuses on the synthesis of mixed metal oxide anodes (MMOs) with the composition Ti/RuO2Sb2O4Ptx (where x = 0, 5, 10 mol) using hybrid microwave irradiation heating. The synthesized electrodes were characterized using scanning electron microscopy, X-ray energy-dispersive analysis, X-ray diffraction, cyclic voltammetry, and electrochemical impedance spectroscopy. These electrodes were then evaluated in both bulk electrolytic and fuel cell tests within a reversible chloralkaline electrochemical cell. The configurations using the electrodes Ti/(RuO2)0.7-(Sb2O4)0.3 and Ti/(RuO2)66.5-(Sb2O4)28.5-Pt5 presented lower onset potential for oxygen and chlorine evolution reactions and reduced resistance to charge transfer compared to the Ti/(RuO2)63-(Sb2O4)27-Pt10 variant. These electrodes demonstrated notable performance in reversible electrochemical cells, achieving Coulombic efficiencies of up to 60% when operating in the electrolytic mode at current densities of 150 mA cm-2. They also reached maximum power densities of 1.2 mW cm-2 in the fuel cell. In both scenarios, the presence of platinum in the MMO coating positively influenced the process. Furthermore, a significant challenge encountered was crossover through the membranes, primarily associated with gaseous Cl2. This study advances our understanding of reversible electrochemical cells and presents possibilities for further exploration and refinement. It demonstrated that the synergy of innovative electrode synthesis strategies and electrochemical engineering can lead to promising and sustainable technologies for energy conversion.

Treatment of Cu/Zn wastes by combined PSU-electrodeposition processes.

This work is focused on the study of the electrodeposition stage of a combined polymer-supported ultrafiltration (PSU)-electrodeposition process for the recovery and selective separation of Cu/Zn mixtures. With this aim, the electrodeposition of Cu/Zn mixtures was evaluated in the presence of ethoxylated polyethylenimine, a polymer that has exhibited good performance in PSU processes in previous studies and whose performance has been fully characterised in the literature. It was determined that at pH values equal to or less than 4, macromolecular complexes formed with both Cu and Zn are weak and electrodeposition can take place very efficiently, with the deposition of copper being strongly favoured. For values of the applied electrical charge equal or greater than 0.1 A h dm(-3), the purity of the copper deposit is greater than 99% for the range of Cu/Zn molar ratios between 1 and infinity. Next, long-term experiments in continuous mode showed that the average deposition rate was close to the maximum rate achieved when operating in discontinuous mode and that the electrodeposition process does not negatively affect the performance of the polymer in the PSU processes.

Combining Soil Vapor Extraction and Electrokinetics for the Removal of Hexachlorocyclohexanes from Soil.

This paper focuses on the evaluation of the mobility of four hexachlorocyclohexane (HCH) isomers by soil vapor extraction (SVE) coupled with direct electrokinetic (EK) treatment without adding flushing fluids. SVE was found to be very efficient and remove nearly 70 % of the four HCH in the 15-days of the tests. The application of electrokinetics produced the transport of HCH to the cathode by different electrochemical processes, which were satisfactorily modelled with a 1-D transport equation. The increase in the electric field led to an increase in the transport of pollutants, although 15 days was found to be a very short time for an efficient transportation of the pollutants to the nearness of the cathode. Loss of water content in the vicinity of the cathode warns about the necessity of using electrokinetic flushing technologies instead of simple direct electrokinetics. Thus, results point out that direct electrokinetic treatment without adding flushing fluids produced low current intensities and ohmic heating that contributes negatively to the performance of the SVE process. No relevant differences were found among the removal of the four isomers, neither in SVE nor in EK processes.

Treatment of gaseous streams polluted with H2S: Comparison of electrolytic and electro-Fenton assisted absorption processes.

H2S is a gaseous compound that contributes to air pollution. In this work, the electrochemical oxidation treatment of gaseous streams polluted with H2S is evaluated using a jet mixer and electrochemical cell device, in which the performance of electrolytic and electro-Fenton assisted absorption processes are compared. Results demonstrate the feasibility of both processes to remove H2S, reaching coulombic efficiencies of nearly 100% in the electrolytic assisted absorption, and 70-80% in the electro-Fenton assisted absorption. Aqueous solutions containing phosphate salts as electrolyte were found to be suitable as absorbents for the process. Efficiency in the cathodic production of H2O2 in these solutions using the experimental device was found to be as high as 32.8% (1.184 mgH2O2/min) at 12 °C and atmospheric pressure. Sequential formation of SO2 and SO3 is obtained by the oxidation of H2S contained in the gas. These species are hydrolysed, and a part remained in the absorbent as SO32- and SO42-, while the rest is dragged in the outlet gas. SO3 production is promoted by electrolytic assisted absorption and polysulphides by the electro-Fenton technology. Low concentrations of elemental sulphur are detected in the solid suspensions formed during the process.

Cork barriers for the remediation of soils polluted with lindane.

The in-situ removal of lindane from spiked soil was studied using cork barriers combined with electrokinetic and ohmic heating soil remediation processes. Both vertical and horizontal cork barriers have been evaluated to retain pollutants mobilized by electro-osmotic flow or volatilized by ohmic heating. Moreover, the addition of surfactant solutions in electrolyte wells has been evaluated to promote the dragging of lindane by electrokinetic fluxes. Results indicated that the drag of lindane by liquid flows is not as important as expected, opposite to what happened with the dragging by gaseous flows. The retention of gaseous lindane was also confirmed in adsorption tests carried out in a column packed with cork granules. The addition of surfactant had a very limited effect on the mobility of lindane, and dragging of this species to the electrode wells or to a permeable reactive barrier. On the contrary, the reactivity of lindane during the electrochemical treatments is relevant due to the electrokinetic basic front promoting the in-situ conversion of lindane into less chlorinated pollutants.

Electrokinetic transport of diesel-degrading microorganisms through soils of different textures using electric fields.

The mobilisation of diesel-degrading microorganisms in soils of three different textures (sandy, clay and silty) using electrokinetic techniques was studied. The mobilisation tests were performed using a laboratory-scale electrokinetic cell in which a synthetic soil column was inserted between the cathode and anode compartments. Microorganisms were located at the anode compartment at the beginning of each assay. A constant cell voltage was applied, and samples were taken from the cathode and anode compartments. Microbial transport through the soil strongly depended on soil particle size. Small particle sizes (silty and clay soil) travelled at low velocities (microbial transport rates of approximately 0.06 and 0.17 cm/min, respectively), while large particle sizes (sandy soil) led to high numbers of microorganisms passing through the soil column. In sandy soil, an increase in the voltage gradient did not increase the quantity of mobilised microorganisms (approximately 10(7) CFU/mL for every voltage gradient applied). For clay and silty soils, a higher voltage gradient led to a higher quantity of microorganisms mobilised to the cathodic compartment and a lower delay time for detecting the presence of microorganisms in the same compartment.

Electrochemical degradation of a methyl paraben and propylene glycol mixture: Interference effect of competitive oxidation and pH stability.

Endocrine disrupting compounds (EDCs) are one of the many classes of harmful pollutants frequently found in water resources. Even at low concentrations, EDCs might accumulate in the organisms and interfere on numerous processes controlled by hormones. Parabens, for example, are preservatives widely used in pharmaceutical and cosmetic industries, but several studies related them to human breast cancer. It is well-known that electrochemical technologies are an efficient alternative for wastewater treatment, promoting the appropriate destruction of EDCs. However, most studies are applied to single target contaminant solutions, which may neglect the impact from co-exited inorganic/organic pollutants. Based on that, this study aimed to elucidate the interfering effects of two target organic contaminants of very different nature during electrochemical mediated process. For that, methyl paraben (MeP) and propylene glycol (PG) were selected as models of aromatic/phenolic and carboxylate compounds versus low-molecular aliphatic alcohols. These two compounds are often together used in preservative blends and cosmetic/pharmaceutical formulations. PG is not a harmful chemical, but it is present in several types of effluents in relatively high concentrations. Thus, it may interfere on the degradation of numerous pollutants of low concentrations. The electrochemical treatment of a mixture containing 100 mg L-1 MeP +1000 mg L-1 PG showed that both contaminants suffered interfering effects. The presence of MeP negatively interfered on PG degradation; the carboxylate compound is more easily oxidized even at lower molecular concentration. On the other hand, the presence of PG showed an unexpected positive effect on MeP degradation, that was not reflected on its mineralization. The results indicate that in addition to the expected effect of anodic competition, polymerization and copolymerization reactions may also occur in the studied system. The use of an acidic buffer medium increased the removal of both contaminants and favored the oxidation pathway over the polymerization. In this case, the increase in the removal was reflected in the mineralization process, which increased up to 6 times when the mixture was treated in the buffered medium.

Enhancement of the Green H2 Production by Using TiO2 Composite Polybenzimidazole Membranes.

This study reports the hydrogen production using TiO2 based composite polybenzimidazole membranes through the SO2 depolarized electrolysis that requires lower energy input than the direct water electrolysis. Composite membranes prepared and studied in this work showed very promising results in terms of proton conductivity, chemical stability, and crossover. Thus, a reduction in SO2 crossover was observed with the increase of the concentration of TiO2, obtaining reductions as high as 42% with the 3.0 wt% TiO2-PBI membrane at 120 °C. Higher hydrogen production rates and Faradaic efficiencies were achieved by all the composite membranes, with an optimum for the 1.0 wt% TiO2-PBI membrane (with this membrane, the production of hydrogen increased a 53% at 110 °C and a 49% at 120 °C as compared with the standard PBI membrane), demonstrated the benefit of the use of composite membranes with respect to the standard one for green hydrogen production.