RESUMEN
1. The levels of protein kinase activity and zymogen granule phosphorylation were studied in the adult rat during stimulus-coupled secretion in vitro. 2. The specific activity of protein kinase associated with intact zymogen granules was 11 pmol [32P]phosphate transferred to histone per min per mg protein. Most of this activity was recovered in purified granule membranes. 2. The addition of 10(-6) M cyclic AMP to a mixture of zymogen granules and the postmicrosomal supernatant resulted in a 5-fold increase in protein kinase activity associated with zymogen granules. The adsorbed activity was eluted from granules by 0.15 M NaCl. Cyclic GMP did not promote protein kinase binding to isolated granules. 4. Incubation of tissues with carbachol (10(-5) M), pancreozymin (0.1 unit/ml), caerulein (10(-8) M) or dibutyryl cyclic AMP (2.10(-4) M) between 2.5 and 60 min did not increase the levels of protein kinase activity in isolated zymogen granules above control values. 5. Protein phosphorylation of zymogen granule membranes and granule content was not detectable in tissues incubated with carbachol, pancreozymin-C-octapeptide, or caerulein. 6. These results suggest that neither the phosphorylation of zymogen granule membrane protein nor the adsorption of protein kinase activity to zymogen granules is an obligatory step in secretion.
Asunto(s)
Precursores Enzimáticos/metabolismo , Páncreas/enzimología , Proteínas Quinasas/metabolismo , Animales , Carbacol/farmacología , Ceruletida/farmacología , Colecistoquinina/farmacología , AMP Cíclico/farmacología , Masculino , Proteínas de la Membrana/metabolismo , Páncreas/efectos de los fármacos , Páncreas/metabolismo , Fosforilación , Ratas , Fracciones Subcelulares/enzimología , Distribución TisularRESUMEN
Zymogen granule membranes from the rat exocrine pancreas displays distinctive, simple protein and glycoprotein compositions when compared to other intracellular membranes. The carbohydrate content of zymogen granule membrane protein was 5-10-fold greater than that of membrane fractions isolated from smooth and rough microsomes, mitochondria and a preparation containing plasma membranes, and 50-100-fold greater than the zymogen granule content and the postmicrosomal supernate. The granule membrane glycoprotein contained primarily sialic acid, fucose, mannose, galactose and N-acetylglucosamine. The levels of galactose, fucose and sialic acid increased in membranes in the following order: rough microsomes less than smooth microsomes less than zymogen granules. Membrane polypeptides were analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The profile of zymogen granule membrane polypeptides was characterized by GP-2, a species with an apparent molecular weight of 74 000. Radioactivity profiles of membranes labeled with [3H]glucosamine or [3H]leucine, as well as periodic acid-Schiff stain profiles, indicated that GP-2 accounted for approx. 40% of the firmly bound granule membrane protein. Low levels of a species similar to GP-2 were detected in membranes of smooth microsomes and the preparation enriched in plasma membranes but not in other subcellular fractions. These results suggest that GP-2 is a biochemical marker for zymogen granules. Membrane glycoproteins of intact zymogen granules were resistant to neuraminidase treatment, while those in isolated granule membranes were readily degraded by neuraminidase. GP-2 of intact granules was not labeled by exposure to galactose oxidase followed by reduction with NaB3H4. In contrast, GP-2 in purified granule membranes was readily labeled by this procedure. Therefore GP-2 appears to be located on the zymogen granule interior.
Asunto(s)
Glicoproteínas/biosíntesis , Proteínas de la Membrana/biosíntesis , Páncreas/metabolismo , Animales , Carbohidratos/análisis , Fraccionamiento Celular , Gránulos Citoplasmáticos/metabolismo , Masculino , Membranas/metabolismo , Peso Molecular , Ratas , Fracciones Subcelulares/metabolismoAsunto(s)
Glicoproteínas/biosíntesis , Hexosiltransferasas/metabolismo , Páncreas/enzimología , Pirofosfatasas/metabolismo , Animales , Isótopos de Carbono , Fraccionamiento Celular , Membrana Celular/análisis , Centrifugación por Gradiente de Densidad , Cromatografía en Capa Delgada , Galactosa , Concentración de Iones de Hidrógeno , Cinética , Masculino , Manganeso/farmacología , Microscopía Electrónica , Microsomas/efectos de los fármacos , Microsomas/enzimología , Mitocondrias/enzimología , Páncreas/citología , Fosfolípidos/análisis , Ratas , Fracciones Subcelulares/enzimología , Tiamina Pirofosfato , Azúcares de Uridina DifosfatoAsunto(s)
Páncreas/metabolismo , Péptidos/metabolismo , Fosfoproteínas/biosíntesis , Proteínas Quinasas/metabolismo , Adenosina Trifosfato/metabolismo , Animales , Calcio/farmacología , Radioisótopos de Carbono , Centrifugación por Gradiente de Densidad , AMP Cíclico/farmacología , Ácido Edético/farmacología , Electroforesis en Gel de Poliacrilamida , Concentración de Iones de Hidrógeno , Leucina/metabolismo , Magnesio/farmacología , Membranas/efectos de los fármacos , Membranas/enzimología , Membranas/metabolismo , Organoides/enzimología , Organoides/metabolismo , Páncreas/citología , Páncreas/enzimología , Radioisótopos de Fósforo , Ratas , Teofilina/farmacologíaAsunto(s)
Glicoproteínas , Proteínas de la Membrana , Páncreas/análisis , Aminoácidos/análisis , Animales , Carbohidratos/análisis , Gránulos Citoplasmáticos/análisis , Perros , Glicoproteínas/aislamiento & purificación , Proteínas de la Membrana/aislamiento & purificación , Membranas/análisis , Ácidos Siálicos/análisisRESUMEN
In fetal rat pancreas cytodifferentiation occurs between day 14 and day 20 of gestation and is accompanied by an exponential increase in the cellular accumulation of tissue specific proteins and an elaboration of the cellular organelles associated with their synthesis and secretion. Evaluation of RNA synthesis by [3H] uridine incorporation into trichloroacetic acid precipitable material showed that during this period the apparent rate of RNA synthesis increased 7.5 fold from 2 x 10(3) dpm/micrograms DNA/h on day 15 to 1.5 x 10(4) dpm/micrograms DNA/h on day 19; [3H] leucine uptake showed that the rate of protein synthesis increased about the same extent with the major difference being that the maximum rate of protein synthesis occurred on day 19, one day after the maximum rate of RNA synthesis. The soluble pyrimidine nucleotide pools decreased from 122 pmol/micrograms DNA on day 14 to 15 pmol/micrograms DNA on day 16 followed by an increase to 104 pmol/micrograms DNA on day 19; the purine nucleotide pools decreased from 367 pmol/micrograms DNA on day 14 to 286 pmol/micrograms DNA on day 16 and then increased to 635 pmol/micrograms DNA on day 19. These values roughly paralleled the transitions observed in the rates of RNA and protein synthesis. Agarose-acrylamide slab gel electrophoresis showed an increase in RNA synthesis and an increase in ribosomal RNA synthesis and processing with cytodifferentiation.
Asunto(s)
Páncreas/embriología , ARN/biosíntesis , Animales , Diferenciación Celular , Electroforesis en Gel de Agar , Electroforesis en Gel de Poliacrilamida , Edad Gestacional , Leucina/metabolismo , Nucleótidos/metabolismo , ARN Ribosómico/biosíntesis , Ratas , Tritio , Uridina/metabolismoRESUMEN
Oil of Mediterranean oregano Oreganum vulgare was orally administered to 14 adult patients whose stools tested positive for enteric parasites, Blastocystis hominis, Entamoeba hartmanni and Endolimax nana. After 6 weeks of supplementation with 600 mg emulsified oil of oregano daily, there was complete disappearance of Entamoeba hartmanni (four cases), Endolimax nana (one case), and Blastocystis hominis in eight cases. Also, Blastocystis hominis scores declined in three additional cases. Gastrointestinal symptoms improved in seven of the 11 patients who had tested positive for Blastocystis hominis.
Asunto(s)
Parasitosis Intestinales/tratamiento farmacológico , Magnoliopsida , Aceites de Plantas/uso terapéutico , Adulto , Amebiasis/tratamiento farmacológico , Animales , Infecciones por Blastocystis/tratamiento farmacológico , Blastocystis hominis , Emulsiones , Endolimax , Entamebiasis/tratamiento farmacológico , Heces/parasitología , HumanosRESUMEN
Peroxynitrite (ONOO-) has been proposed as a mediator of gut inflammation and as an inducer of cell death by apoptosis. Phytolens (PHY), a water-soluble extract of polyphenolic antioxidants from nonsoy legumes (Biotics Research Corp, patent pending), was evaluated as a cytoprotective agent in human colonic (T84) and murine macrophage (RAW 264.7) cell lines. In the antioxidant testing, PHY showed a significant free radical scavenging ability against 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and superoxide (O2.) radicals with an IC50 of 4.44 and 5.87 microg/ml against DPPH and O2., respectively. Apoptosis (DNA fragmentation) was measured by an ELISA technique. Cells were exposed to oxidative stress by treating them with peroxynitrite (100-300 microM) for 4 h in the presence and absence of PHY. Peroxynitrite elicited a dose-dependent increase in DNA fragmentation in both cell lines compared to the control group receiving decomposed ONOO-. PHY (10, 30, or 50 microg/ml) significantly attenuated the degree of apoptosis in T84 cells induced by ONOO- (P < 0.05). PHY (10-100 microg/ml) did not directly affect T84 cell viability or induce apoptosis after 4 h or overnight exposure. RAW 264.7 cells exposed to PHY alone (>30 microg/ml) for 4 h displayed decreased cell viability (P < 0.05) and increased apoptosis (P < 0.05). Phytolens may have beneficial effects on inflammation by attenuating peroxynitrite-induced apoptosis. The sparing of epithelial cells while compromising the viability of macrophages suggests that PHY may be beneficial in autoimmune disorders.