Congenital ureteral ectopia in continent and incontinent-related Entlebucher mountain dogs: 13 cases (2006-2009).

BACKGROUND: Ectopic ureters (EUs) associated with varying combinations of urinary incontinence, hydronephrosis, and urinary tract infection have been identified in related North American Entlebucher Mountain Dogs. OBJECTIVES: To characterize the disease phenotype in affected dogs and evaluate possible modes of inheritance. ANIMALS: Twenty client-owned Entlebucher Mountain Dogs. Nine dogs had clinical signs of urinary tract disease. METHODS: Prospective case series in which 17 dogs were evaluated with excretory urography, ultrasonography, and urethrocystoscopy. Three additional dogs were evaluated by necropsy alone. Clinical and pedigree histories from 165 North American Entlebuchers were compiled for analysis. RESULTS: Eleven female and 2 male dogs were found to have EUs. Six females and 1 male were continent. Bilateral intravesicular ectopic ureters (IVEUs) were identified in 9 dogs, bilateral extravesicular ectopic ureters (EVEUs) in 3 dogs, and 1 dog had IVEU and EVEU. Hydronephrosis was identified in 5 dogs, 3 of which had bilateral IVEUs. Two necropsied dogs had bilateral hydronephrosis with presumed ureterovesical junction obstruction associated with chronic granulation tissue or lymphoplasmacytic inflammation. Twenty-six dogs with EUs were identified in the pedigree. Because of incomplete penetrance, mode of inheritance could not be determined. CONCLUSIONS AND CLINICAL IMPORTANCE: Ureteral ectopia is common in North American Entlebucher Mountain Dogs and clinical signs alone could not reliably predict disease phenotype. EVEUs were associated with urinary incontinence and occasionally hydronephrosis. IVEUs were clinically silent or associated with hydronephrosis. Further analyses are necessary to confirm and characterize the hereditary nature of the disorder.

Sodium zeolite a supplementation and its impact on the skeleton of dairy calves.

Twenty calves were placed on study at 3 days of age and were placed according to birth order into one of two groups: SS, which received 0.05% BW sodium zeolite A (SZA) added to their milk replacer, and CO, which received only milk replacer. Blood samples were taken on days 0, 30, and 60 for osteocalcin (OC) and deoxypyridinoline (DPD) analysis. On day 60, the calves were euthanized, and synovial fluid, articular cartilage, and both fused metacarpals were collected for bone quality analyses such as architecture and mechanical properties, mineral composition, and glycosaminoglycan concentration. There were no differences in OC concentrations because of treatment (p = 0.12), and CO calves had lower DPD concentrations than SS calves (p = 0.01), but the OC-to-DPD ratio was not different between treatments (p = 0.98). No differences in bone architecture or mechanical properties were detected. SZA supplementation increased cortical bone (p = 0.0002) and articular cartilage (p = 0.05) aluminum content. Glycosaminoglycan concentrations were not different in synovial fluid or cartilage. Supplementation of SZA appeared to alter the rate of bone turnover without altering bone strength. Aluminum concentrations in the bone and cartilage increased, which may be a concern, although the long-term consequences of such remain to be determined.

Adolescent attachment and psychopathology.

The relationships among attachment classification, psychopathology, and personality traits were examined in a group of 60 psychiatrically hospitalized adolescents. The concordance of attachment classification was examined in 27 adolescent-mother pairs. Both adolescent and maternal attachment status were overwhelmingly insecure and were highly concordant. Adolescents showing a dismissing attachment organization were more likely to have a conduct or substance abuse disorder, narcissistic or antisocial personality disorder, and self-reported narcissistic, antisocial, and paranoid personality traits. Adolescents showing a preoccupied attachment organization were more likely to have an affective disorder, obsessive-compulsive, histrionic, borderline or schizotypal personality disorder, and self-reported avoidant, anxious, and dysthymic personality traits. The results support a model of development of psychopathology based partially on relational experiences with parents.

Digital angiography of the feet of horses.

OBJECTIVE: To describe the vascular anatomy of the palmar digital artery and its major branches in the equine foot and to quantify the diameter of these vessels by use of digital angiograms. Sample Population-6 thoracic limbs obtained from 6 horses. PROCEDURE: Distal portions of each limb were perfused with aerated Krebs-Henseleit solution. Digital angiograms were acquired in standing and lateral recumbent positions, following an intra-arterial injection of iopamidol. Select vessels were measured on radiographic views, and values were corrected for magnification. RESULTS: The palmar digital artery tapered from 2.28 mm at the coronary region to 1.61 mm at the entrance to the solar canal, and the major arterial branches ranged in diameter from 0.71 to 1.42 mm in the standing position. CONCLUSIONS AND CLINICAL RELEVANCE: Digital angiography is useful for imaging small vessels, but penumbra limits the image resolution of the macrovasculature of the foot. The palmarodorsal projection is more useful for evaluation of the terminal arch and solar branches, but 2 projections are necessary for a thorough examination of the foot. Image magnification, position of horse, and vascular response to contrast medium must be considered in the quantitative assessment of vessel diameter. Digital angiography may be performed in clinical cases and research models for examination of vascular perfusion of the distal portion of the limb.

Computed tomographic study of the effect of a tongue-tie on hyoid apparatus position and nasopharyngeal dimensions in anesthetized horses.

OBJECTIVE: To determine the effect of manual tongue protrusion on the dimensions of the hyoid apparatus, nasopharynx, and oropharynx in anesthetized horses. tongue protrusion on the dimensions of the hyoid. ANIMALS: 5 adult horses. PROCEDURE: Horses were anesthetized and positioned in sternal recumbency for 2 sequential computed tomographic (CT) scans. Images were acquired with the tongue in a natural position inside the mouth. Then, the tongue was pulled rostrally and secured, and a second CT scan was performed. Dorsoventral length of the hyoid apparatus and angles of the basisphenoid, basihyoid, and ceratohyoid were measured on 3-dimensional reconstructed CT images. Cross-sectional diameters and areas of the nasopharynx and oropharynx were determined on reformatted images in the transverse and longitudinal planes, using osseous landmarks for consistency. Results were tested between the 2 groups to determine significant differences. RESULTS: We were unable to detect a significant difference between any of the lengths or angles of the hyoid apparatus measured with or without rostral protrusion of the tongue. Similarly, nasopharyngeal and oropharyngeal diameters and cross-sectional areas were not significantly different with or without rostral protrusion of the tongue. CONCLUSIONS AND CLINICAL RELEVANCE: Tying the tongue rostrally out of a horse's mouth did not influence the position of the hyoid apparatus or dimensions of the nasopharynx or oropharynx in anesthetized horses. Currently, no data suggest that application of a tongue-tie is effective for maintaining stability and patency of the nasopharyngeal or orolaryngeal airways in horses during races.

Arthrocentesis of the temporomandibular joint in adult horses.

OBJECTIVE: To develop a method for arthrocentesis of the temporomandibular joint in adult horses. ANIMALS: 7 equine cadaver heads and 6 clinically normal adult horses. PROCEDURE: Fluoroscopy, contrast radiography, and computed tomography were used on cadaver specimens to locate the temporomandibular joint, identify externally palpable landmarks for joint access, guide needle placement into the joint, and illustrate regional anatomy. The arthrocentesis technique was performed on 6 live healthy adult horses to determine efficacy and safety of this procedure. RESULTS: Externally palpable structures were identified as landmarks for temporomandibular arthrocentesis, including the lateral border of the condylar process of the mandible, the zygomatic process of the temporal bone, and the lateral pericapsular fat pad. Arthrocentesis was successful in all 6 joints in the live horses, and no complications developed. CONCLUSIONS AND CLINICAL RELEVANCE: The technique identified will improve the ability to examine and treat the temporomandibular joint in horses.

Surgical treatment of progressive ethmoidal hematoma aided by computed tomography in a foal.

A progressive ethmoidal hematoma (PEH) was treated successfully in a 4-week-old Belgian filly by surgical removal, using a frontonasal bone flap. The filly had respiratory stridor, epistaxis, and facial enlargement over the left paranasal sinuses, which had progressively increased in size since birth. Computed tomographic images of the head obtained with the foal under general anesthesia were useful in determining the extent and nature of the soft-tissue mass and planning surgical intervention. On the basis of the histologic appearance of the mass, a diagnosis of PEH was made. Twelve months after surgery, the facial appearance was normal and the abnormal appearance of the ethmoid region on endoscopic evaluation was less obvious, with return of the nasal septum to a normal position. Progressive ethmoidal hematoma is uncommon and, to our knowledge, has not been reported in a neonate. Clinical signs of PEH in this foal were atypical because of the rapid enlargement of the mass, extent of facial deformity, and minimal epistaxis and interoperative hemorrhage.

Exercising stall-housed gestating gilts: effects on lameness, the musculo-skeletal system, production, and behavior.

Lameness in breeding-age gilts and sows is a major cause of culling, resulting in increased economic losses and welfare concerns. This study determined if exercise during gestation would affect the musculo-skeletal system, production variables, and behavior. Gilts were blocked by BW and assigned to 1 of 3 treatment groups: control (n = 10; no exercise), low exercise (n = 14; 122 m/d for 5 d/wk), and high exercise (n = 14; 122 m/d for 2 d/wk and 427 m/d for 3 d/wk). All gilts were stall-housed during gestation, and gilts were exercised between d 35 and 110 of gestation. Lameness score, BCS, BW, and blood were taken at multiple points before gestation, and during gestation and lactation. Blood serum was analyzed for carboxy-terminal telopeptide of type I collagen. Sow lying behavior was recorded for 3 d after farrowing. Farrowing data included litter weight and size at birth and weaning, and preweaning mortality. After weaning, 38 sows were slaughtered and muscles and the bones of the left fore- and hind-limbs were harvested. Bone density and quality were determined by computed tomography (CT) scans, dual energy x-ray scans, and bone-breaking force tests. The control group took longer to lie down than both exercise groups, and the low exercise group took longer to lie down than the high exercise group (P < 0.05). The number of pigs weaned was greater in the high exercise group than the control group (P < 0.05). Piglet preweaning mortality was greatest in the control group compared with both exercise groups (P < 0.05). The low exercise treatments exhibited a greater bone density (CT) in the humerus, radius, and tibia compared with that of the control group (P < 0.05). The bone density (CT) of the humerus in the low exercise group was greater than that of the high exercise group (P = 0.03). Breaking force in the humerus and femur was greater (P < 0.05) in the low exercise group than the control group. Breaking force in the tibia of the high exercise group was greater than the control group (P = 0.01). The tibia of both the low and high exercise groups had a greater breaking force (P < 0.05) than the control group. Although there was no benefit of exercise on lameness, differences in bone density and quality, lying behavior, and piglet survivability may provide useful insight into alternative housing for sows.

Impact of supplement withdrawal and wheat middling inclusion on bone metabolism, bone strength, and the incidence of bone fractures occurring at slaughter in pigs.

The objective of this study was to determine if supplement withdrawal (omission of dietary vitamin and trace mineral premixes and 2/3 of inorganic P) 28-d preslaughter and the feeding of wheat middlings (dietary concentrations of 5, 15, or 30% from weaning to 16 kg, 16 to 28 kg, and 28 kg to slaughter, respectively) affect bone metabolism, bone strength, bone density, and the incidence of bone fractures at slaughter in pigs. Crossbred barrows (n = 64) were assigned to a 2 x 2 factorial arrangement of treatments (with or without supplement withdrawal, and with or without wheat middlings). Serum was collected on d 0, 14, and 27 of the preslaughter withdrawal period to determine changes in the concentrations of osteocalcin, an indicator of bone formation, and pyridinoline, an indicator of bone resorption. The serum osteocalcin and pyridinoline concentrations on d 14 and 27 were analyzed as change from the d-0 concentration. At slaughter, radiographs of the lumbar vertebrae and of the right and left femurs were taken to determine the incidence of bone fractures. Third metacarpal bones were analyzed for bone mineral density, peak load, ultimate shear stress, and percent ash. Supplement withdrawal increased (P < 0.05) serum osteocalcin and pyridinoline concentrations, indicating an increase in osteoblast activity and bone resorption. Supplement withdrawal decreased (P < 0.01) bone mineral density, peak load, ultimate shear stress, and percent ash of the metacarpal bones. Dietary wheat middling inclusion did not alter bone quality. Neither supplement withdrawal nor wheat middling inclusion affected the incidence of bone fractures at slaughter. The results of this study indicate that removing inorganic P, vitamin premix, and trace mineral premix for 28 d preslaughter increases bone turnover and decreases bone quality.

Diagnostic imaging in canine pheochromocytoma.

The diagnosis of pheochromocytoma is challenging due to the variable presentations of patients affected with this neoplasm. This report describes four dogs with pheochromocytoma in which radiography, ultrasonography and computed tomography (CT) were key diagnostic tools. Computed tomography was the most useful imaging modality for assessment of size, shape and margination of the tumor. CT findings for the pheochromocytoma included a large, irregularly shaped mass in the dorsal midabdomen with multiple foci of low attenuation dispersed in hyperdense, highly vascular tissue. This neoplasm often invades the caudal vena cava and other surrounding organs therefore, although a pheochromocytoma may be identified in the absence of clinical signs, it should not be considered an incidental lesion. The unpredictable growth rate and potential for invasion of major vessels warrants serious consideration. Follow-up ultrasound and CT examinations are recommended for patients with adrenal masses that do not undergo surgical excision.

Image magnification in digital fluoroscopy.

Image magnification is inherent in radiography. In digital fluoroscopy, the three components of magnification are geometric, electronic and photographic. In this study, the total magnification factor of a digital imaging system was determined by two methods, 1) comparison of measurements of a known object to its image and 2) calculation of geometric, electronic and photographic magnification from the imaging system specifications. Both methods were employed for various focal-film distances, image intensifier tube modes and laser printer formats. Results of these two methods were different due to the detrimental effect of penumbra on image quality with increasing magnification. If a radiographic image is to be used to approximate object size, then a technique should be used that will minimize magnification. In digital fluoroscopy this is achieved with the shortest object-film distance (assuming a fixed focal-object distance), largest image intensifier mode and greatest number of images per sheet of film.

Squamous epithelial proliferation in the jaws of mink fed diets containing 3,3',4,4',5-pentachlorobiphenyl (PCB 126) or 2,3,7,8-tetrachlorodibenzo-P-dioxin (TCDD).

Previous research has shown that ingestion of 3,3',4,4',5-pentachlorobiphenyl (PCB 126) by juvenile mink (kits) caused a lesion in the mandible and maxilla that consisted of proliferation of sQuamous epithelium in the periodontal ligament, osteolysis of adjacent alveolar bone, and loose and displaced teeth. Similar, but less severe changes, developed in adult mink fed 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The present study was conducted to compare similarities and differences of the lesion within the jaws of mink fed these 2 polyhalogenated hydrocarbons. Diets containing 24 ppb PCB 126 or 2.4 ppb TCDD were fed to 6-w-old kits for 36 d. Similar diets were fed to 12-w-old kits for 35 d. Some of these mink were then fed untreated feed for an additional 50 d. All mink treated with PCB 126 or TCDD had reductions in body weight gains which were more severe in the 6-w-old kits than the 12-week-old kits. By 28 days of exposure, many of the 6- and 12-week-old mink treated with PCB 126 or TCDD had loose and displaced incisor teeth. Canine teeth were grossly more prominant. Radiographs showed maxillary and mandibular osteolysis with lysis of the lamina dura in treated mink. Withdrawal of the toxicants from the diets of the 12-w-old mink failed to alleviate the lesions, which continued to be progressively more severe.

Influence of trotting and supplemental weight on metacarpal bone development.

The use of weight-training to alter bone strength has not been investigated in horses. Recognising that bone responds to loading, we studied the effect of carrying weight on bone development during training. Seventeen horses were divided into 3 groups: controls exercised counterclockwise in a free-flow exerciser; the weight group performed the same exercise carrying progressively increasing weight up to 45 kg and the weight supplement group also received a myo-anabolic supplement. Radiographic equivalence measure of bone mineral content of zones of the third metacarpi (MCIII) was determined on 4 occasions: baseline (Day 108), pre-conditioning following 108 days stall confinement (Day 0), mid-conditioning (Day 39) and end-conditioning (Day 78). Stall confinement resulted in loss of mineral in lateral and medial cortices of both MCIII. During conditioning, weight-carrying increased mineral deposition in lateral and medial cortices of MCIII of the left (inside) leg compared with controls. In the right leg, controls had lower mineral content of the lateral cortex at Day 39 than weight-carrying horses but no differences between treatments were recorded at Day 78. Markers of bone metabolism did not change from baseline to pre-conditioning, but increased from Day 0 to 39 and 78 for all groups. This study demonstrates the benefits to bone mineral deposition in the third metacarpi of carrying weight when trained at low speeds and re-emphasises the potential for bone loss when not given sufficient exercise.

Comparison of bone mineral content and biochemical markers of bone metabolism in stall- vs. pasture-reared horses.

Sixteen Arabian yearlings were assigned randomly to 2 groups, confined to stall and pastured, to investigate the effects of confinement vs. pasture-rearing on bone mineral content and biochemical markers of bone metabolism over a 140 day period. Following an 84 day pretraining period, 6 horses from each group were selected randomly to complete a 56 day training period. Serum osteocalcin concentrations were determined from blood samples collected every 14 days. Urinary deoxypyridinoline concentrations and mineral content of the third metacarpus, as determined by lateral and medial radiographic bone aluminum equivalency (RBAE), were determined every 28 days from 24 h urine samples and radiographs of the left forelimb, respectively. In comparison with starting values, lateral RBAE was lower in the confined horses at Day 28 and remained lower throughout most of the project, while pastured horses had increasing lateral RBAE. Horses kept in stalls had lower medial RBAE at Day 28 than pasture-reared horses. Medial RBAE tended to remain lower in confined horses than in pastured horses throughout most of the project. The onset of training failed to negate the loss of mineral. Serum osteocalcin concentrations were lower and urinary deoxypyridinoline concentrations were higher in the confined horses at Days 14 and 28, respectively, compared with the pastured horses, and subsequently returned to baseline. These results suggest that housing yearling/2-year-old horses in stalls may be associated with a loss of bone mineral content in comparison with horses maintained on pasture.

Comparison of trochlear block recession and trochlear wedge recession for canine patellar luxation using a cadaver model.

OBJECTIVE: To compare trochlear block recession (TBR) to trochlear wedge recession (TWR) with regards to patellar depth (percentage of patellar volume under the trochlear ridges), patellar articular contact, percentage of recessed trochlear surface area, and resistance to patellar luxation. STUDY DESIGN: In vitro computed tomography (CT) and biomechanical evaluation using a cadaver model. SAMPLE POPULATION: Twelve normal, large-breed canine cadavers. METHODS: Bilateral pelvic limb specimens with intact stifle joints were mounted on a positioning device. The femoral trochlear ridges were reduced to provide a standard shallow trochlea. TBR or TWR was performed to a standard depth randomly on paired specimens. CT and biomechanical evaluations were performed pre- and postoperatively in both an extended (148 degrees ) and flexed (113 degrees ) stifle position. CT images were digitized and measurements made using an image-analysis software program. Biomechanical testing consisted of applying 40 degrees of internal tibial rotation and documenting patellar luxation. RESULTS: The change in trochlear depth (depth of recession) was not significantly different between groups. In the extended stifle position (patella in the proximal trochlea), patellar depth and patellar articular contact with the recessed trochlea were significantly greater after TBR compared with TWR. The percentage of recessed trochlear surface area was significantly greater after TBR compared with TWR. In the extended position, a smaller percentage of the patellae luxated within 40 degrees of internal tibial rotation after TBR compared with TWR. CONCLUSIONS: TBR increases proximal patellar depth, increases patellar articular contact with the recessed proximal trochlea, recesses a larger percentage of trochlear surface area, and results in a greater resistance to patellar luxation in an extended position as compared with TWR. CLINICAL RELEVANCE: TBR may help limit the development of stifle DJD in dogs treated for canine patellar luxation.