The effect of combined pollution by PAHs and heavy metals on the topsoil microbial communities of Spolic Technosols of the lake Atamanskoe, Southern Russia.

The contamination with organic and inorganic pollutants changes significantly soil microbial community structure. These shifts indicate anthropogenic pressure and help to discover new possibilities for soil remediation. In this study, the microbial community structure of Spolic Technosols formed at the territory of a former industrial sludge reservoir near the Kamensk-Shakhtinsky (Southern Russia) was studied using a metagenomics approach. The studied soils contain high concentrations of heavy metals (HM) (up to 72,900 mg kg-1) and 16 priority polycyclic aromatic hydrocarbons (PAHs) (up to 6670 mg kg-1). Its microbial communities demonstrate an excellent adaptability level reflected in their complexity and diversity. As shown by the high values of alpha diversity indices (Shannon values up to 10.1, Chao1 values from 1430 to 4273), instead of decreasing quantitatively and qualitatively on the systemic level, microbial communities tend to undergo complex redistribution. Regardless of contamination level, the share of Actinobacteria and Proteobacteria was consistently high and varied from 20 to 50%. Following the results of the Mann-Whitney U test, there were significant changes of less abundant phyla. The abundance of oligotrophic bacteria from Gemmatimonadetes and Verrucomicrobia phyla and autotrophic bacteria (e.g., Nitrospira) decreased due to the high PAH's level. And abundance of Firmicutes and amoebae-associated bacteria such as TM6 and soil Chlamydia increased in highly contaminated plots. In the Spolic Technosols studied, the influence of factors on the microbial community composition decreased from PAHs concentration to soil characteristics (organic carbon content) and phylum-phylum interactions. The high concentrations of HMs influenced weakly on the microbial community composition.

Application of XAFS and XRD methods for describing the copper and zinc adsorption characteristics in hydromorphic soils.

Modeling metal sorption in soils is of great importance to predict the fate of heavy metals and to assess the actual risk driven from pollution. The present study focuses on adsorption of HM ions on two types of hydromorphic soils, including calcaric fluvisols loamic and calcaric fluvic arenosols. The individual and competitive adsorption behaviors of Cu and Zn on soils and soil constituents are evaluated comprehensively. It is established that the sorption processes were best described with the Langmuir model. The results suggest that the calcaric fluvic arenosols are more vulnerable to heavy metal input compared to fluvisols loamic. In all cases, Cu had a higher range of values of the adsorption process parameters relative to Zn. The Zn is likely to be the most critical environmental factor in such soils since it exhibited a decreased sorption under competitive conditions. The retention mechanisms of HM in hydromorphic soils are considered. Based on theoretical calculations of ion activity in soil solutions using solubility diagrams of Cu and Zn compounds, the possibility of precipitation of Cu hydroxide and Zn carbonate in the studied soils is shown. Direct physical methods of nondestructive testing (XAFS and XRD) are applied to experimentally prove the formation of these HM compounds on the surface of montmorillonite, the dominant mineral in hydromorphic soils, and calcite. Thus, the combination of both physicochemical methods and direct physical methods can provide a large amount of real information about the mechanisms of HM retain with solid phases.

Phylogenetic analysis of hyperaccumulator plant species for heavy metals and polycyclic aromatic hydrocarbons.

Increasing concentration of heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) in the soil may impose a serious threat to living organisms due to their toxicity and the ability to accumulate in plant tissues. The present review focuses on the phylogenetic relationships, sources, biotransformation and accumulation potential of hyperaccumulators for the priority HMs and PAHs. This review provides an opportunity to reveal the role of hyperaccumulators in removal of HMs and PAHs from soils, to understand the relationships between pollutants and their influence on the environment and to find potential plant species for soil remediation. The phylogenetic analysis results showed that the hyperaccumulators of some chemicals (Co, Cu, Mn, Ni, Zn, Cd) are clustered on the evolutionary tree and that the ability to hyperaccumulate different pollutants can be correlated either positively (Cd-Zn, Pb-Zn, Co-Cu, Cd-Pb) or negatively (Cu-PAHs, Co-Cd, Co-PAHs, Ni-PAHs, Cu-Ni, Mn-PAHs). Further research needs to be extended on the focus of commercializing the techniques including the native hyperaccumulators to remediate the highly contaminated soils.

Data on the temporal changes in soil properties at the emergency crash site of the launch vehicle 'Soyuz-FG' in Kazakhstan.

On October 11, 2018, in the Ulytau region of the Republic of Kazakhstan, the Soyuz-FG launch vehicle carrying a crewed MS-10 spacecraft failed, creating two areas where rocket propellants spilled and soil condition was monitored in 2018, 2019, 2022 and 2023. This article presents data on the content of pollutants, namely unsymmetrical dimethylhydrazine (UDMH), nitrosodimethylamine (NDMA) and total petroleum hydrocarbons (TPH), total N, organic carbon, exchangeable Ca and Mg, water-soluble NO3-, NO2-, HCO3-, CO3 2-, SO4 2-, Cl-, K +, Na+, pH values, cation exchange capacity and electrical conductivity of the water extract in disturbed and background Aridisols (more than 200 samples in total). This data set contains information on interseasonal (autumn 2022 and spring 2023) differences in the content and vertical differentiation of some soil properties in Aridisols in Central Kazakhstan. In autumn, the content of TPH, water-soluble Cl- and SO4 2- and alkalinity from CO3 2- is 1.4, 235, 201, and 2 times higher, respectively, and the content of total N and water-soluble NO3 - and NO2 -, alkalinity from HCO3 - is 2.4, 1.4, 6.4 and 1.9 times lower, respectively (p < 0.05). In spring and autumn, the content of exchangeable Ca and Mg, cation exchange capacity did not differ significantly. The presented materials can be used to optimize restoration of disturbed arid ecosystems and future monitoring work at sites of regular landing of the first stages and emergency crash sites of launch vehicles.

Data on the temporal changes in soil properties and microbiome composition after a jet-fuel contamination during the pot and field experiments.

The soil response to a jet-fuel contamination is uncertain. In this article, original data on the influence of a jet-fuel spillage on the topsoil properties are presented. The data set is obtained during a one-year long pot and field experiments with Dystric Arenosols, Fibric Histosols and Albic Luvisols. Kerosene loads were 1, 5, 10, 25 and 100 g/kg. The data set includes information about temporal changes in kerosene concentration; physicochemical properties, such as рН, moisture, cation exchange capacity, content of soil organic matter, available P and K, exchangeable NH4 +, and water-soluble NO3 -; and biological properties, such as biological consumption of oxygen, and cellulolytic activity. Also, we provide sequencing data on variable regions of 16S ribosomal RNA of microbial communities from the respective soil samples.

Review on the environmental impact of emissions from space launches: a case study for areas affected by the Russian space programme.

In this paper, we summarised the main field-based approaches and a large volume of data on the territories affected by the Russian space programme conducted at Plesetsk, Baikonur, and Vostochny cosmodromes. Influence of space transportation on the ozone layer depletion, as well as on environmental and human health, is negligible. The environmental consequences of rocket and space activities within launch pads and the terrestrial drop zones of jettisoned first stages of launch vehicles are allowable. Moreover, it is negligible in the second stage drop zones. Unsymmetrical dimethylhydrazine pollution is local and does not result in the formation of ecological disaster zones because ecosystems restore by themselves. Influence of rocket launches on the mesospheric cloud formation is short-time or/and insignificant. The environmental impact of space transportation by the Russian space programme on the terrestrial ecosystems is well-studied. To approve or to disprove these findings similar researches should be carried out in other terrestrial and aquatic drop zones affected due to the space programmes of other countries.

Protocol of conjugate evaluation of the biological activity of soils in terms of cellulolytic activity and biological consumption of oxygen.

The article presents protocols for determining the biological activity of kerosene-contaminated soils in terms of two indicators, i.e. cellulolytic activity and biological consumption of oxygen. A method for determining the cellulolytic activity of soils is based on measuring the rate of cellulose decomposition in situ. Model test objects (linen fragments 10 × 20 cm weighing 4-6 g) were put in the root layer of soil. A month later, the linen was removed from soil and its weight loss was measured. Cellulolytic activity was estimated by the weight loss of readily hydrolysable organic matter (RHOM) per day (mg/g RHOM per day). The method for determining the biological consumption of oxygen of water was adapted for soils. The indicator characterizes the ability of microorganisms to oxidize organic substances using oxygen for 5 days. The analytic procedure includes taking a soil sample, preparing the suspension (the ratio of soil to distilled water is at least 1:10) and after 5 days measuring the concentration of unspent dissolved oxygen using the oxygen meter. The proposed methods give reproducible and reliable results on the biochemical activity of soil microorganisms in a wide range of soils, e.g. Retisols, Arenosols and Histosols, including those under hydrocarbon pollution.

Temporal change in plant communities and its relationship to soil salinity and microtopography on the Caspian Sea coast.

The gradual drying up of saltwater bodies creates habitats that are characterised by changing environmental conditions and might be available only for a subset of plants from the local flora. Using two terrestrial areas with different ages on the Caspian Coast as a chronosequence, we investigated factors including microtopography, ground water level and soil salinity that drive plant community succession after the retreat of the sea. Vegetation of the two key sites appearing after the retreat of the Caspian Sea about 365 and 1412 years ago were compared in terms of both evolutionary and ecological traits of plants. Both edaphic conditions and vegetation differed between the two sites with harsher edaphic conditions and more xerophytes on the elder site. Species that grew only in the 'early' site were dispersed across the phylogenetic tree, but their loss on the 'late' site was not random. Species that grew only on the 'late' site were phylogenetically clustered. On the level of microtopography, elevated spots were more densely populated in the 'early' site than lowered spots, but on the 'late' site the situation was opposite. The main edaphic factors that drive the difference in vegetation composition between the two sites are likely salinity and moisture. During environmental changes, different plant traits are important to survive and to appear in the community de novo. Microtopography is important for forming plant communities, and its role changes with time.

Data on the soil and vegetation properties at the small gully catchment area: Steppe region of Kalmykia Republic (South Russia).

In rural areas, research on the environment in native (untaught) soils is important to understand the rate of pedogenesis and to prevent the problems associated with hidden huger. In this article, original data on vegetation, chemical properties and elemental and mineralogical composition of Kastanozems (Protosalic, Siltic) and Hypersalic Solonetz (Siltic) of the small gully catchment (2 ha in total) located at the NE Ergeni Upland (Western Kalmykia, Russia) were presented. Vegetation was described and cut off (to characterize an aboveground biomass) at 13 key plots of 1 × 1 m. The list of species of the small gully catchment area amounts to 23 species (predominantly, perennial herbs) belonging to 13 families and 11 orders. The main dominants are Artemisia lerchiana, A. austriaca, Festuca valesiaca and Poa bulbosa. Soils were described and sampled in 11 cross-sections and two key plots (0 - 10 cm topsoil sampling). In soil water extracts (79 samples in total), electrical conductivity (EC) and pH were measured. In soil samples, particle size distribution, soil organic carbon and CaCO3 contents, total concentration of all the macro elements, some trace (Cl, Nb, Rb, Th, Y, Zr) and potentially toxic elements (As, Co, Cr, Cu, Ni, Pb, Sr, V, and Zn) were described. Moreover, the concentration of three mobile fractions of elements (Li, Be, B, Na, Mg, Al, Si, P, S, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Sr, Ba, Cd, Pb) measured using Inductively Coupled Plasma Atomic Emission Spectrometry (AES-ICP) was presented. Geochemical indexes of weathering (R - Silica/Alumina, CIW - Chemical Index of Weathering, CIA - Chemical Index of Alteration, WIP - Weathering Index of Parker, PWI -Product of Weathering Index, Vogt Ratio, PIA - Plagioclase Index of Alteration, STI - Silica-Titanium Index, B/A - Bases/Alumina, B/R - Bases/R2O3, Si/R - Silica/R2O3, Weathering indexes WI-1 and WI-2, Si/Ses - Silica/Sesquioxides, Si/Fe - Silica/Iron, a - Potassium/Sodium, ba-1 - (Potassium-Sodium)/Alumina, ba-2 - (Calcium-Magnesium)/Alumina, Ba - (Potassium-Sodium-Calcium)/Alumina) were calculated. In 12 bulk soil samples from Kastanozems and Solonetz, mineralogy (X-Ray diffractometry, the Rietveld full-pattern fitting method for quantitative analysis) was described. Data obtained can be used for more confident identification of pollution sources and pollutants' migration routes, as well as for more effective land-use management, calculating the required doses of nutrients and for adaptation of land use.

The Influence of Kerosene on Microbiomes of Diverse Soils.

One of the most important challenges for soil science is to determine the limits for the sustainable functioning of contaminated ecosystems. The response of soil microbiomes to kerosene pollution is still poorly understood. Here, we model the impact of kerosene leakage on the composition of the topsoil microbiome in pot and field experiments with different loads of added kerosene (loads up to 100 g/kg; retention time up to 360 days). At four time points we measured kerosene concentration and sequenced variable regions of 16S ribosomal RNA in the microbial communities. Mainly alkaline Dystric Arenosols with low content of available phosphorus and soil organic matter had an increased fraction of Actinobacteriota, Firmicutes, Nitrospirota, Planctomycetota, and, to a lesser extent, Acidobacteriota and Verrucomicobacteriota. In contrast, in highly acidic Fibric Histosols, rich in soil organic matter and available phosphorus, the fraction of Acidobacteriota was higher, while the fraction of Actinobacteriota was lower. Albic Luvisols occupied an intermediate position in terms of both physicochemical properties and microbiome composition. The microbiomes of different soils show similar response to equal kerosene loads. In highly contaminated soils, the proportion of anaerobic bacteria-metabolizing hydrocarbons increased, whereas the proportion of aerobic bacteria decreased. During the field experiment, the soil microbiome recovered much faster than in the pot experiments, possibly due to migration of microorganisms from the polluted area. The microbial community of Fibric Histosols recovered in 6 months after kerosene had been loaded, while microbiomes of Dystric Arenosols and Albic Luvisols did not restore even after a year.

Impact of kerosene pollution on ground vegetation of southern taiga in the Amur Region, Russia.

The present study is the field experiment on kerosene pollution impact on southern taiga plant communities. Experimental sites were located in a mixed forest, a deciduous forest, a sedge fen and a wet meadow within the Amur Region of the Russian Far East. Kerosene loads from 1 to 500 g/kg of soil were applied to 50 × 50 cm plots in three replications and their effects on number of species and projective cover of ground vegetation were analysed in 1.5 months and 1 year after exposure. Statistical analyses of data included Student's t-test, Friedman ANOVA and correlation coefficient (r). Phylogenetic analysis was carried out for herbaceous plants on experimental plots. The highest susceptibility to kerosene pollution was found in the mixed forest, where the edificator species (Pteridium aquilinum subsp. pinetorum) was significantly suppressed by the kerosene load of only 1 g/kg of soil. Wetland communities regenerated faster than ground vegetation of forests, especially, in tests with high (>25 g/kg) kerosene loads. The wet meadow community was the most resistant to kerosene pollution, i.e., despite significant decreases in projective cover and number of species after exposure to kerosene loads of 5 and 25 g/kg in the first season, it had the highest regeneration success in the next season. In our study, the kerosene load of 25 g/kg of soil was the threshold level of pollution, above which there were significant structural changes in the studied plant communities. Depending on their abilities to resist kerosene pollution and to regenerate in the next year, dominant species of the studied plant communities were arranged in the following ascending order: Pteridium aquilinum ssp. pinetorum, Convallaria keiskei < Carex cespitosa, Calamagrostis purpurea < Lespedeza bicolor < Vaccinium uliginosum.

Data on the soilscape and vegetation properties at the key site in the NW Caspian sea coast, Russia.

Research on the environment in recent soils is important to understand geochemical processes in coastal landscapes and the rate of pedogenesis. In this article, we present original data on Gleyic Solonchaks (Loamic) and vegetation described at the eastern part of the Terek-Kuma lowland (Northern Dagestan, Russia). At the key site of 45 × 30 m released from water 293±13 years calBP, we described vegetation at 345 plots of 2 × 2 m (4 m2) and soil properties in 58 auger holes and 2 pedons, the latter characterizing a typical microhigh with Tamarix and a microlow with saltworts. The flora of the sites amounts to 32 species (predominantly, halophytes) belonging to 11 families. Shrubs represented by tamarixes are the dominant. Under their crowns, dense herb and grass microcommunities with a predominance of tall Puccinellia gigantea occur. Sparse stunted halophytic plants (Petrosimonia, Frankenia, Puccinellia) occupy open habitats between shrubs. In soil water extracts from auger holes (696 samples in total), we measured electrical conductivity (EC) and pH. In 49 soil samples from pedons, we described particle size distribution, total concentration of macro elements (Al, Ca, Fe, K, as well as Mg, Mn, P, Ti, and Si) and trace elements (As, Co, Cr, Cu, Ni, Pb, Sr, and Zn), EC, pH, basicity (HCO3 - and CO3 2-) as well as the content of cations (Ca2+, Mg2+, Na+, and K +) and anions (SO4 2- and Cl-) in soil water extracts. Gleyic Solonchaks (Loamic) with bulk density of 1.35±0.12 g/cm3 (mean and standard deviation) contain SiO2 69±8%> Al2O3 11.8 ±â€¯3.5 and CaO 7.5 ±â€¯2.5%, Fe2O3 3.6 ±â€¯1.4%, K2O 2.0 ±â€¯0.3 and MgO 1.9 ±â€¯0.4%> TiO2 0.62±0.25%> P2O5 0.14±0.06% and MnO 713±268 mg/kg> Sr 481±262 mg/kg > Cr 79±9 mg/kg > V 76±36, Zn 68±31, Cu 62±10, and Ni 50±17 mg/kg, Co 32±6 mg/kg> Pb 11±6 mg/kg> As 5.6 ±â€¯1.4 mg/kg. The particle-size distribution is (WRB system,%): clay 13±5, fine silt 34±12, coarse silt 30±18, as well as very fine sand 11±10, fine sand 7.3 ±â€¯10.5, medium sand 3.5 ±â€¯5.8, coarse sand 0.9 ±â€¯3.2, and very coarse sand 0.08±0.31 (n = 38). Soil water extract has EC 9.4 ±â€¯4.1 dSm/m (soils: water ratio of 1:2.5, n = 713), contains Na+ 15.9 ±â€¯7.0 > Ca2+7.3 ±â€¯5.0 and Mg2+ 7.3 ±â€¯3.1 > K + 0.30±0.20 cmol(eq)/kg, as well as Cl- 15.7 ±â€¯7.3 and SO4 2- 14.6 ±â€¯7.9 > HCO3 - 0.55±0.15 > CO3 2 -< 0.01 cmol(eq)/kg, and has pH 7.9 ±â€¯0.3 (soils: water ratio of 1:5, n = 21). In soil paste, pH is 8.3 ±â€¯0.2 (n = 461). Data obtained can be used for more confident identification of pollution sources and pollutants' migration routes and more effective conservation and remediation of human-affected soils at the Caspian Sea coast.

Dataset on heavy metal content in background soils of the three gully catchments at Western Siberia.

The criteria used include heavy metal (HM) levels in background soils of different countries and territories (Australia, China, Finland, North America, Northern Europe, and Western Siberia) and their threshold concentration values for soils of residential and/or agricultural areas in soil quality standards of Canada (soil quality guidelines), Germany (Trigger Values), the Netherlands (Serious Risk Concentrations), Russia (Maximum Permissible Concentrations), and the USA (Regional Screening Levels). The Retisols, Phaeozems and Chernozems of Western Siberia are characterized by the following range of mean concentrations of heavy metals in topsoil (in mg kg-1): Pb 5-35, Cu 5-100, V 5-180, Cr 5-212, Ni 7-100, Zn 10-135, Mn 50-1800, and Ba 373-1360.

Data on the concentration of fractions and the total content of chemical elements in catenae within a small catchment area in the Trans Urals, Russia.

Research on migration of chemical elements (ChEs) in soils is important for the understanding of geochemical processes in polluted and undisturbed landscapes. In this article, we report original data on Anthric Chernozems and Anthric Planosols within a small gully's catchment area in the Trans Urals (Russia). Mean total concentrations of 24 ChEs and content of mobile fractions (F1 - exchangeable, F2 - bound within organo-mineral complexes and F3 - bound with Fe and Mn hydroxides) of 61 ChEs including macro elements (Al, Ca, Fe, K, Mg, Mn, Na, P, Ti, S, Si), heavy metals (Ba, Co, Cr, Cu, Ni, Pb, Rb, Sr, Th, U, V, Zn), trace elements (Ag, As, B, Be, Bi, Br, Cd, Cs, Ge, Hf, Li, Mo, Nb, Pd, Sb, Sc, Se, Sn, Ta, Te, Tl, W, Zr) and rare earth elements (Ce, Er, Eu, Gd, La, Lu, Nd, Pr, Sm, Tb, Tm, Dy, Ho, Y, Yb) are determined from in a total of 60 samples from topsoil and subsoil of Anthric Chernozems and Anthric Planosols. The data obtained also include pH-value, total organic carbon content (TOC), seven particle-size classes (<2, 2-6.3, 6.3-20, 20-63, 63-200, 200-630 and 630-2000 µm), electrical conductivity and chemical composition (cations and anions) of water extracts as well as soil mineralogical composition.