RESUMEN
The binaural masking level difference (BMLD) is a phenomenon whereby a signal that is identical at each ear (S0), masked by a noise that is identical at each ear (N0), can be made 12-15 dB more detectable by inverting the waveform of either the tone or noise at one ear (Sπ, Nπ). Single-cell responses to BMLD stimuli were measured in the primary auditory cortex of urethane-anesthetized guinea pigs. Firing rate was measured as a function of signal level of a 500 Hz pure tone masked by low-passed white noise. Responses were similar to those reported in the inferior colliculus. At low signal levels, the response was dominated by the masker. At higher signal levels, firing rate either increased or decreased. Detection thresholds for each neuron were determined using signal detection theory. Few neurons yielded measurable detection thresholds for all stimulus conditions, with a wide range in thresholds. However, across the entire population, the lowest thresholds were consistent with human psychophysical BMLDs. As in the inferior colliculus, the shape of the firing-rate versus signal-level functions depended on the neurons' selectivity for interaural time difference. Our results suggest that, in cortex, BMLD signals are detected from increases or decreases in the firing rate, consistent with predictions of cross-correlation models of binaural processing and that the psychophysical detection threshold is based on the lowest neural thresholds across the population.
Asunto(s)
Estimulación Acústica/métodos , Corteza Auditiva/fisiología , Umbral Auditivo/fisiología , Localización de Sonidos/fisiología , Potenciales de Acción/fisiología , Animales , Femenino , Cobayas , MasculinoRESUMEN
Responses of neurons to binaural, harmonic complex stimuli in urethane-anesthetized guinea pig inferior colliculus (IC) are reported. To assess the binaural integration of harmonicity cues for sound segregation and grouping, responses were measured to harmonic complexes with different fundamental frequencies presented to each ear. Simultaneously gated harmonic stimuli with fundamental frequencies of 125 Hz and 145 Hz were presented to the left and right ears, respectively, and recordings made from 96 neurons with characteristic frequencies >2 kHz in the central nucleus of the IC. Of these units, 70 responded continuously throughout the stimulus and were excited by the stimulus at the contralateral ear. The stimulus at the ipsilateral ear excited (EE: 14%; 10/70), inhibited (EI: 33%; 23/70), or had no significant effect (EO: 53%; 37/70), defined by the effect on firing rate. The neurons phase locked to the temporal envelope at each ear to varying degrees depending on signal level. Many of the cells (predominantly EO) were dominated by the response to the contralateral stimulus. Another group (predominantly EI) synchronized to the contralateral stimulus and were suppressed by the ipsilateral stimulus in a phasic manner. A third group synchronized to the stimuli at both ears (predominantly EE). Finally, a group only responded when the waveform peaks from each ear coincided. We conclude that these groups of neurons represent different "streams" of information but exhibit modifications of the response rather than encoding a feature of the stimulus, like pitch.
Asunto(s)
Percepción Auditiva , Colículos Inferiores/fisiología , Animales , Potenciales Evocados Auditivos , Femenino , Cobayas , Colículos Inferiores/citología , Masculino , Neuronas/fisiologíaRESUMEN
Animal models of tinnitus allow us to study the relationship between changes in neural activity and the tinnitus percept. Here, guinea pigs were subjected to unilateral noise trauma and tested behaviourally for tinnitus 8 weeks later. By comparing animals with tinnitus with those without, all of which were noise-exposed, we were able to identify changes unique to the tinnitus group. Three physiological markers known to change following noise exposure were examined: spontaneous firing rates (SFRs) and burst firing in the inferior colliculus (IC), evoked auditory brainstem responses (ABRs), and the number of neurons in the cochlear nucleus containing nitric oxide synthase (NOS). We obtained behavioural evidence of tinnitus in 12 of 16 (75%) animals. Both SFRs and incidences of burst firing were elevated in the IC of all noise-exposed animals, but there were no differences between tinnitus and no-tinnitus animals. There were significant decreases in ipsilateral ABR latencies in tinnitus animals, contrary to what might be expected with a small hearing loss. Furthermore, there was an ipsilateral-contralateral asymmetry in NOS staining in the ventral cochlear nucleus (VCN) that was only apparent in tinnitus animals. Tinnitus animals had a significantly greater number of NOS-containing neurons on the noise-exposed side, whereas no-tinnitus animals did not. These data suggest that measuring NOS in the VCN and recording ABRs supplement behavioural methods for confirming tinnitus in animals, and that nitric oxide is involved in plastic neural changes associated with tinnitus.
Asunto(s)
Potenciales Evocados Auditivos del Tronco Encefálico , Acúfeno/fisiopatología , Animales , Núcleo Coclear/citología , Núcleo Coclear/metabolismo , Núcleo Coclear/fisiopatología , Femenino , Cobayas , Pérdida Auditiva Provocada por Ruido/complicaciones , Colículos Inferiores/citología , Colículos Inferiores/metabolismo , Colículos Inferiores/fisiopatología , Masculino , Neuronas/metabolismo , Óxido Nítrico Sintasa/genética , Óxido Nítrico Sintasa/metabolismo , Tiempo de Reacción , Acúfeno/etiologíaRESUMEN
A differential response to sound frequency is a fundamental property of auditory neurons. Frequency analysis in the cochlea gives rise to V-shaped tuning functions in auditory nerve fibres, but by the level of the inferior colliculus (IC), the midbrain nucleus of the auditory pathway, neuronal receptive fields display diverse shapes that reflect the interplay of excitation and inhibition. The origin and nature of these frequency receptive field types is still open to question. One proposed hypothesis is that the frequency response class of any given neuron in the IC is predominantly inherited from one of three major afferent pathways projecting to the IC, giving rise to three distinct receptive field classes. Here, we applied subjective classification, principal component analysis, cluster analysis, and other objective statistical measures, to a large population (2826) of frequency response areas from single neurons recorded in the IC of the anaesthetised guinea pig. Subjectively, we recognised seven frequency response classes (V-shaped, non-monotonic Vs, narrow, closed, tilt down, tilt up and double-peaked), that were represented at all frequencies. We could identify similar classes using our objective classification tools. Importantly, however, many neurons exhibited properties intermediate between these classes, and none of the objective methods used here showed evidence of discrete response classes. Thus receptive field shapes in the IC form continua rather than discrete classes, a finding consistent with the integration of afferent inputs in the generation of frequency response areas. The frequency disposition of inhibition in the response areas of some neurons suggests that across-frequency inputs originating at or below the level of the IC are involved in their generation.
Asunto(s)
Vías Auditivas/fisiología , Colículos Inferiores/fisiología , Neuronas/fisiología , Estimulación Acústica , Animales , Cobayas , Neuronas/clasificaciónRESUMEN
First spike latency has been suggested as a source of the information required for fast discrimination tasks. However, the accuracy of such a mechanism has not been analyzed rigorously. Here, we investigate the utility of first spike latency for encoding information about the location of a sound source, based on the responses of inferior colliculus (IC) neurons in the guinea pig to interaural phase differences (IPDs). First spike latencies of many cells in the guinea pig IC show unimodal tuning to stimulus IPD. We investigated the discrimination accuracy of a simple latency code that estimates stimulus IPD from the preferred IPD of the single cell that fired first. Surprisingly, despite being based on only a single spike, the accuracy of the latency code is comparable to that of a conventional rate code computed over the entire response. We show that spontaneous firing limits the capacity of the latency code to accumulate information from large neural populations. This detrimental effect can be overcome by generalizing the latency code to estimate the stimulus IPD from the preferred IPDs of the population of cells that fired the first n spikes. In addition, we show that a good estimate of the neural response time to the stimulus, which can be obtained from the responses of the cells whose response latency is invariant to stimulus identity, limits the detrimental effect of spontaneous firing. Thus, a latency code may provide great improvement in response speed at a small cost to the accuracy of the decision.
Asunto(s)
Potenciales de Acción/fisiología , Cobayas/fisiología , Colículos Inferiores/fisiología , Neuronas/fisiología , Localización de Sonidos/fisiología , Transmisión Sináptica/fisiología , Animales , Femenino , MasculinoRESUMEN
We reconstructed the intrinsic axons of 32 neurons in the guinea pig inferior colliculus (IC) following juxtacellular labeling. Biocytin was injected into cells in vivo, after first analyzing physiological response properties. Based on axonal morphology there were two classes of neuron: (1) laminar cells (14/32, 44%) with an intrinsic axon and flattened dendrites confined to a single fibrodendritic lamina and (2) translaminar cells (18/32, 56%) with axons that terminated in two or more laminae in the central nucleus (ICc) or the surrounding cortex. There was also one small, low-frequency cell with bushy-like dendrites that was very sensitive to interaural timing differences. The translaminar cells were subdivided into three groups of cells with: (a) stellate dendrites that crossed at least two laminae (8/32, 25%); (b) flattened dendrites confined to one lamina and that had mainly en passant axonal swellings (7/32, 22%) and (c) short, flattened dendrites and axons with distinctive clusters of large terminal boutons in the ICc (3/32, 9%). These terminal clusters were similar to those of cortical basket cells. The 14 laminar cells all had sustained responses apart from one offset response. Almost half the non-basket type translaminar cells (7/15) had onset responses while the others had sustained responses. The basket cells were the only ones to have short-latency (7-9 ms), chopper responses and this distinctive temporal response should allow them to be studied in more detail in future. This is the first description of basket cells in the auditory brainstem, but more work is required to confirm their neurotransmitter and precise post-synaptic targets.
Asunto(s)
Colículos Inferiores , Animales , Axones , Núcleos Cerebelosos , Dendritas , Cobayas , NeuronasRESUMEN
Psychophysical studies show a slower response to changes in the specifically binaural input than to changes in the monaural input (binaural sluggishness). However, there is disagreement about the time course. Tracking changes in a target yields fast time constants, while detecting a constant target against a varying background yields the slowest. Changes in the binaural properties of a target are tracked up to high rates by cells in the midbrain. Indeed cells respond rapidly to a step change and then the firing rate slowly adapts. These experiments, though, are analogues of psychophysical experiments that give the faster time constants. Sluggishness should be more apparent physiologically in a binaural masking paradigm, detecting a short tone in a noise masker with a step change in masker correlation: the small change in firing rate due to the signal must be detected against the adapting firing rate change caused by the step change in the masker. However, in 40 inferior colliculus cells in the anesthetized guinea pig, in a direct analogue of the psychophysical masking paradigm, measuring thresholds for short tones across a transition in a binaural masker (e.g., from N0S0 to NpiS0) provided little evidence of sluggishness within individual cells despite masking level differences in these cells comparable with previous data. Previous studies of physiological correlates of binaural masking level difference suggested that different psychophysical thresholds arise from different populations of cells. This suggests the hypothesis that sluggishness may result from a change in focus between the different populations of cells signaling threshold in different binaural configurations rather than within the intrinsic properties of the cells themselves.
Asunto(s)
Estimulación Acústica , Lateralidad Funcional/fisiología , Colículos Inferiores/fisiología , Localización de Sonidos/fisiología , Animales , Electrodos Implantados , Potenciales Evocados Auditivos/fisiología , Cobayas , RuidoRESUMEN
One of the fundamental questions of auditory research is how sounds are segregated because, in natural environments, multiple sounds tend to occur at the same time. Concurrent sounds, such as two talkers, physically add together and arrive at the ear as a single input sound wave. The auditory system easily segregates this input into a coherent perception of each of the multiple sources. A common feature of speech and communication calls is their harmonic structure and in this report we used two harmonic complexes to study the role of the corticofugal pathway in the processing of concurrent sounds. We demonstrate that, in the inferior colliculus (IC) of the anesthetized guinea pig, deactivation of the auditory cortex altered the temporal and/or the spike response to the concurrent, monaural harmonic complexes. More specifically, deactivating the auditory cortex altered the representation of the relative level of the complexes. This suggests that the auditory cortex modulates the representation of the level of two harmonic complexes in the IC. Since sound level is a cue used in the segregation of auditory input, the corticofugal pathway may play a role in this segregation.
Asunto(s)
Estimulación Acústica , Corteza Auditiva/fisiología , Vías Auditivas/fisiología , Colículos Inferiores/fisiología , Potenciales de Acción/fisiología , Animales , Femenino , Cobayas , Masculino , Modelos Animales , Percepción de la Altura Tonal/fisiologíaRESUMEN
The minimal change in a stimulus property that is detectable by neurons has been often quantified using the receiver operating characteristic (ROC) curve, but recent studies introduced the use of the related Fisher information (FI). Whereas ROC analysis and FI quantify the information available for discriminating between two stimuli, global aspects of the information carried by a neuron are quantified by the mutual information (MI) between stimuli and responses. FI and MI have been shown to be related to each other when FI is large. Here the responses of neurons recorded in the inferior colliculus of anesthetized guinea pigs in response to ensembles of sounds differing in their interaural time differences (ITDs) or binaural correlation (BC) were analyzed. Although the FI is not uniformly large, there are strong relationships between MI and FI. Information-theoretic measures are used to demonstrate the importance of the non-Poisson statistics of these responses. These neurons may reflect the maximization of the MI between stimuli and responses under constraints on the coded stimulus range and the range of firing rates. Remarkably, whereas the maximization of MI, in conjunction with the non-Poisson statistics of the spike trains, is enough to create neurons whose ITD discrimination capabilities are close to the behavioral limits, the same rule does not achieve single-neuron BC discrimination that is as close to behavioral performance.
Asunto(s)
Colículos Inferiores/fisiología , Neuronas/fisiología , Localización de Sonidos/fisiología , Estimulación Acústica , Algoritmos , Animales , Interpretación Estadística de Datos , Lateralidad Funcional/fisiología , Cobayas , Teoría de la Información , Probabilidad , Curva ROCRESUMEN
One of the main central processes affecting the cortical representation of conspecific vocalizations is the collateral output from the extended motor system for call generation. Before starting to study this interaction we sought to compare the characteristics of calls produced by stimulating four different parts of the brain in guinea pigs (Cavia porcellus). By using anaesthetised animals we were able to reposition electrodes without distressing the animals. Trains of 100 electrical pulses were used to stimulate the midbrain periaqueductal grey (PAG), hypothalamus, amygdala, and anterior cingulate cortex (ACC). Each structure produced a similar range of calls, but in significantly different proportions. Two of the spontaneous calls (chirrup and purr) were never produced by electrical stimulation and although we identified versions of chutter, durr and tooth chatter, they differed significantly from our natural call templates. However, we were routinely able to elicit seven other identifiable calls. All seven calls were produced both during the 1.6 s period of stimulation and subsequently in a period which could last for more than a minute. A single stimulation site could produce four or five different calls, but the amygdala was much less likely to produce a scream, whistle or rising whistle than any of the other structures. These three high-frequency calls were more likely to be produced by females than males. There were also differences in the timing of the call production with the amygdala primarily producing calls during the electrical stimulation and the hypothalamus mainly producing calls after the electrical stimulation. For all four structures a significantly higher stimulation current was required in males than females. We conclude that all four structures can be stimulated to produce fictive vocalizations that should be useful in studying the relationship between the vocal motor system and cortical sensory representation.
Asunto(s)
Encéfalo/fisiología , Estimulación Acústica/métodos , Animales , Percepción Auditiva/fisiología , Estimulación Eléctrica/métodos , Femenino , Cobayas , Masculino , Neuronas/fisiología , Vocalización Animal/fisiologíaRESUMEN
We measured interaural time difference (ITD) sensitivity of 72 cells in the inferior colliculus of the anaesthetised guinea pig as a function of frequency and interaural level difference (ILD). For many units there was a "null" frequency, where varying the ILD made no difference to the position of the peak of the ITD sensitivity. This null frequency was not necessarily at the characteristic frequency (CF): it occurred at CF in less than a third of the neurons for which we had sufficient data (14/50). Equally often, the null occurred below (15/50) and less often, above CF (8/50). The remaining (13/50) neurons showed clear phase changes, but these were erratic or parallel and no null could be attributed. In 33 of the 37 neurons with an identifiable null frequency, the peak ITD moved towards the recording side with increasing ILD, for frequencies above the null, and away for frequencies below the null. The changes in ITD sensitivity expressed as phase were maximally about 0.2-0.3 cycles. Many of the changes in response phase with ILD are in the same direction and magnitude as changes in the phase locking with sound level in auditory nerve fibres. Thus, these changes in phase sensitivity at the basilar membrane and auditory nerve are maintained through to ITD tuning in the IC. This is consistent with a coincidence detection mechanism. However, some of the more complex phenomena which we observe are consistent with convergence at the IC.
Asunto(s)
Colículos Inferiores/fisiología , Localización de Sonidos/fisiología , Estimulación Acústica , Animales , Femenino , Cobayas , Colículos Inferiores/citología , Masculino , Neuronas/fisiología , Factores de TiempoRESUMEN
The discrimination of a change in a stimulus is determined both by the magnitude of that change and by the variability in the neural response to the stimulus. When the stimulus is itself noisy, then the relative contributions of the neural (intrinsic) and stimulus induced variability becomes a critical question. We measured the contribution of intrinsic neural noise and interstimulus variability to the discrimination of interaural time differences (ITDs) and interaural correlation (IAC). We measured discharge rate versus characteristic frequency (CF) tone ITD functions, and CF-centered narrowband noise ITD and IAC functions in interleaved blocks in the same units in the inferior colliculus of urethane-anesthetized guinea pigs. Ten "frozen" tokens of noise were synthesized and the responses to each token were separately analyzed to allow the relative contributions of intrinsic and stimulus variability to be assessed. ITD and IAC discrimination thresholds were determined for a simulated two-interval forced-choice experiment, based on the firing rate distributions, using receiver operating characteristic analysis. On average, between stimulus variability contributed 19% (range, 1.5-30%) of the variance in noise ITD discrimination and 27% (range, 3-50%) in IAC discrimination. Noise ITD thresholds were slightly higher than tone ITD thresholds. Taking the mean of the thresholds for individual noise tokens gave a similar result to pooling across all noise tokens. This implies that although the stimulus induced variability is measurable, it is insignificant in relation to the intrinsic noise in ITD and IAC discrimination.
Asunto(s)
Percepción Auditiva/fisiología , Audición/fisiología , Colículos Inferiores/fisiología , Animales , Cobayas , Ruido , Factores de TiempoRESUMEN
Sensitivity to changes in the interaural time difference (ITD) of 50 msec tones was measured in single units in the inferior colliculus of urethane-anesthetized guinea pigs. ITD functions were measured with 100 repeats and fine spacing (100 points per cycle). The just noticeable difference (jnd) for ITD was determined using receiver operating characteristic (ROC) analysis of the spike-count distribution at each ITD. The jnd became progressively smaller as the signal frequency increased from 50 to 800 Hz but became unmeasurable above 1 kHz. The lowest jnds (30 microsec) were comparable with human jnds, indicating that there is sufficient information in the firings of individual neurons to permit discrimination without obligatory pooling. ROC analysis requires the choice of a reference ITD from which the jnd may be found by stepping the target ITD through the ITD function. For each neuron the reference was chosen to minimize the jnd. The lowest jnd was usually for ipsilateral leading references, near the minimum of the ITD function where the variance was also low, but where the slope was nearing its steepest. This was despite the peak of the ITD function occurring for contralateral leading stimuli. When the reference ITD was on midline, a jnd could be obtained by looking for firing rates either greater or smaller than the firing rate at midline. The lower jnd was usually obtained by looking for a decrease in firing rate. As duration increased, jnds either decreased or increased, depending on unit type, whereas when level increased, jnds generally increased.
Asunto(s)
Umbral Auditivo/fisiología , Umbral Diferencial/fisiología , Colículos Inferiores/fisiología , Neuronas/fisiología , Tiempo de Reacción/fisiología , Animales , Cobayas , Modelos Lineales , Discriminación de la Altura Tonal/fisiología , Curva ROC , Procesamiento de Señales Asistido por Computador , Localización de Sonidos/fisiologíaRESUMEN
Sensitivity to changes in the interaural correlation of 50-ms bursts of narrowband or broadband noise was measured in single neurons in the inferior colliculus of urethane-anaesthetized guinea pigs. Rate vs. interaural correlation functions (rICFs) were measured using two methods. These methods compensated in different ways for the inherent variance in interaural correlation between tokens with the same expected correlation. The shape of all rICFs could be best described by power functions allowing them to be summarized by two parameters. Most rICFs were best fit by a power below 2, indicating that they were only slightly nonlinear. However, there were a few fitted functions that had a power of 3-6, indicating marked curvature. Modeling results indicate that the nonlinearity of the majority of rICFs was explicable in terms of the monaural transduction stages; however, some of the rICFs with power greater than 2 require either multiple inputs to the coincidence detector or additional nonlinearities to be included in the model. Discrimination thresholds were estimated at reference correlations of -1, 0, and +1 using receiver operating characteristic (ROC) analysis of the spike-count distribution at each correlation. Thresholds spanned the full possible range, from a minimum of 0.1 to the maximum possible of 2. Thresholds were generally highest with a reference correlation of -1, intermediate with a reference of 0, and lowest with a reference correlation of +1. Thresholds were lowest for the most steeply sloped rICFs, but thresholds were not strongly correlated to the spike rate variance. The lowest thresholds occurred using narrowband noise that was compensated for internal delays, but they were still about three times larger than human psychophysical thresholds measured using similar stimuli. The data suggest that, unlike pure tone interaural time difference, discrimination of a population measure is required to account for behavioral interaural correlation discrimination performance.
Asunto(s)
Colículos Inferiores/citología , Colículos Inferiores/fisiología , Neuronas/fisiología , Localización de Sonidos/fisiología , Anestesia , Animales , Vías Auditivas , Umbral Auditivo/fisiología , Discriminación en Psicología/fisiología , Cobayas , Modelos NeurológicosRESUMEN
Guinea pigs produce the low-frequency purr or rumble call as an alerting signal. A digitised example of the call was presented to anaesthetised guinea pigs via a closed sound system while recording from the primary auditory cortex. The exemplar used in this study had 9 regular phrases each spaced with their centres about 80 ms apart. Low-frequency (1.1 kHz) units responded best to the call but within this population there were four separate groups: (1) cells that responded vigorously to many or all of the 9 phrases; (2) cells that gave an onset response; (3) cells that only responded to a click embedded in the call; (4) cells that did not respond. Particular response types were often grouped together. Thus when orthogonal electrode tracks were used most units gave a similar response. There was no correlation between the type of response and the cortical depth. A similar range of response types was also found in the thalamus and there was no evidence of a distinct response in the cortex that was due to intracortical processing. Cells in the cortex were able to represent the temporal structure of the purr with the same fidelity as cells in the thalamus.
Asunto(s)
Estimulación Acústica , Corteza Auditiva/fisiología , Percepción Auditiva/fisiología , Cobayas/fisiología , Vocalización Animal/fisiología , Animales , Análisis por Conglomerados , Femenino , Humanos , Masculino , Tiempo de Reacción , Análisis de Regresión , Espectrografía del SonidoRESUMEN
Considerable circumstantial evidence suggests that cells in the ventral cochlear nucleus, that respond predominantly to the onset of pure tone bursts, have a stellate morphology and project, among other places, to the dorsal cochlear nucleus. The characteristics of such cells make them leading candidates for providing the so-called "wideband inhibitory input" which is an essential part of the processing machinery of the dorsal cochlear nucleus. Here we use juxtacellular labeling with biocytin to demonstrate directly that large stellate cells, with onset responses, terminate profusely in the dorsal cochlear nucleus. They also provide widespread local innervation of the anteroventral cochlear nucleus and a small innervation of the posteroventral cochlear nucleus. In addition, some onset cells project to the contralateral dorsal cochlear nucleus.
Asunto(s)
Vías Auditivas/citología , Nervio Coclear/citología , Núcleo Coclear/citología , Colículos Inferiores/citología , Animales , Vías Auditivas/fisiología , Axones/fisiología , Forma de la Célula , Nervio Coclear/fisiología , Núcleo Coclear/fisiología , Dendritas/fisiología , Potenciales Evocados Auditivos , Femenino , Cobayas , Colículos Inferiores/fisiología , MasculinoRESUMEN
At the level of the brainstem, precise temporal information is essential for some aspects of binaural processing, while at the level of the cortex, rate and place mechanisms for neural coding seem to predominate. However, we now show that precise timing of steady-state responses to pure tones occurs in the primary auditory cortex (AI). Recordings were made from 163 multi-units in guinea pig AI. All units increased their firing rate in response to pure tones at 100 Hz and 46 (28%) gave sustained responses which were synchronised with the stimulus waveform (phase-locking). The phase-locking units were clustered together in columns. Phase-locking was generally strongest in layers III and IV but was also recorded in layers I, II and V. Good phase-locking was observed over a range of 60-250 Hz: some units (30%) were narrow band while others (37%) were low-pass (33% were not determined). Phase-locking strength was also influenced by sound level: some units showed monotonic increases in strength with level and others were non-monotonic. Ten of the units provided a good temporal representation of the fundamental frequency (270 Hz) of a guinea pig vocalisation (rumble) and may be involved in analysing communication calls.
Asunto(s)
Corteza Auditiva/fisiología , Percepción Auditiva/fisiología , Estimulación Acústica , Animales , Corteza Auditiva/anatomía & histología , Potenciales Evocados Auditivos/fisiología , Femenino , Cobayas , Masculino , Vocalización Animal/fisiologíaRESUMEN
It has been suggested that the considerable noise in single-cell responses to a stimulus can be overcome by pooling information from a large population. Theoretical studies indicated that correlations in trial-to-trial fluctuations in the responses of different neurons may limit the improvement due to pooling. Subsequent theoretical studies have suggested that inherent neuronal diversity, i.e., the heterogeneity of tuning curves and other response properties of neurons preferentially tuned to the same stimulus, can provide a means to overcome this limit. Here we study the effect of spike-count correlations and the inherent neuronal heterogeneity on the ability to extract information from large neural populations. We use electrophysiological data from the guinea pig Inferior-Colliculus to capture inherent neuronal heterogeneity and single cell statistics, and introduce response correlations artificially. To this end, we generate pseudo-population responses, based on single-cell recording of neurons responding to auditory stimuli with varying binaural correlations. Typically, when pseudo-populations are generated from single cell data, the responses within the population are statistically independent. As a result, the information content of the population will increase indefinitely with its size. In contrast, here we apply a simple algorithm that enables us to generate pseudo-population responses with variable spike-count correlations. This enables us to study the effect of neuronal correlations on the accuracy of conventional rate codes. We show that in a homogenous population, in the presence of even low-level correlations, information content is bounded. In contrast, utilizing a simple linear readout, that takes into account the natural heterogeneity, even of neurons preferentially tuned to the same stimulus, within the neural population, one can overcome the correlated noise and obtain a readout whose accuracy grows linearly with the size of the population.
Asunto(s)
Potenciales de Acción/fisiología , Colículos Inferiores/fisiología , Modelos Neurológicos , Red Nerviosa/fisiología , Neuronas/fisiología , Animales , Vías Auditivas/fisiología , Cobayas , Localización de Sonidos/fisiologíaRESUMEN
Tinnitus, the perception of sound in the absence of an external stimulus, is a particularly challenging condition to demonstrate in animals. In any animal model, objective confirmation of tinnitus is essential before we can study the neural changes that produce it. A gap detection method, based on prepulse inhibition of the whole-body startle reflex, is often used as a behavioural test for tinnitus in rodents. However, in the guinea pig the whole-body startle reflex is subject to rapid habituation and hence is not an ideal behavioural measure. By contrast, in this species the Preyer or pinna reflex is a very reliable indicator of the startle response and is much less subject to habituation. We have developed a novel adaptation of the gap detection paradigm, which uses the Preyer reflex to measure the startle response, rather than whole-body movement. Using this method, we have demonstrated changes in gap detection, in guinea pigs where tinnitus had been induced by the administration of a high dose of salicylate. Our data indicate that the Preyer reflex gap detection method is a reliable test for tinnitus in guinea pigs.
Asunto(s)
Conducta Animal/fisiología , Modelos Animales de Enfermedad , Reflejo de Sobresalto/fisiología , Acúfeno/diagnóstico , Estimulación Acústica , Animales , Femenino , Cobayas , MasculinoRESUMEN
The central nucleus of the inferior colliculus (IC) is organized into a series of fibro-dendritic laminae, orthogonal to the tonotopic progression. Many neurons have their dendrites confined to one lamina while others have dendrites that cross over a number of laminae. Here, we have used juxtacellular labeling in urethane anesthetized guinea pigs to visualize the cells with biocytin and have analyzed their response properties, in order to try and link their structure and function. Out of a sample of 38 filled cells, 15 had dendrites confined within the fibro-dendritic laminae and in 13 we were also able to reconstruct their local axonal tree. Based on dendritic morphology they were subdivided into flat or less flat; small, medium, or large; elongated or disk-shaped cells. Two of the elongated cells had many dendritic spines while the other cells had few or none. Twelve of the cells had their local axonal tree restricted to the same lamina as their dendrites while one cell had its dendrites in a separate lamina from the axon. The axonal plexus was more extensive (width 0.7-1.4 mm) within the lamina than the dendrites (width generally 0.07-0.53 mm). The intrinsic axons were largely confined to a single lamina within the central nucleus, but at least half the cells also had output axons with two heading for the commissure and five heading into the brachium. We were able to identify similarities in the physiological response profiles of small groups of our filled cells but none appeared to represent a homogeneous morphological cell type. The only common feature of our sample was one of exclusion in that the onset response, a response commonly recorded from IC cells, was never seen in laminar cells, but was in cells with a stellate morphology. Thus cells with laminar dendrites have a wide variety of physiological responses and morphological subtypes, but over 90% have an extensive local axonal tree within their local lamina.