RESUMEN
Myocardial infarction (MI) is a life-threatening disease. In this study, we examined the anti-mitochondrial damaging effects of sinapic acid (SA) in isoproterenol (ISO)-induced myocardial infarcted rats. Myocardial infarcted rats were prepared by injecting ISO (100 mg/kg body weight) on the 9th and 10th day. Rats were pretreated and cotreated with SA (12 mg/kg body weight) orally, daily for 10 days. A considerable increase in serum lactate dehydrogenase, creatine kinase, myoglobin, and cardiac troponin-T was noticed in the ISO-induced rats. ISO also significantly amplified lipid peroxidation and calcium ions, and depleted the antioxidant system and mitochondrial enzymes in rat's heart mitochondria. SA treatment improved the distorted above- mentioned biochemical parameters in ISO-treated rats with its anti-mitochondrial damaging effects. This ultrastructural study on heart mitochondria and in vitro studies also confirmed the effects of SA. The current findings are suggestive of SA's cardioprotective effects.
Asunto(s)
Agonistas Adrenérgicos beta/toxicidad , Cardiotónicos/farmacología , Ácidos Cumáricos/farmacología , Isoproterenol/toxicidad , Mitocondrias Cardíacas/efectos de los fármacos , Infarto del Miocardio/patología , Animales , Biomarcadores/sangre , Masculino , Mitocondrias Cardíacas/ultraestructura , Infarto del Miocardio/inducido químicamente , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas WistarRESUMEN
The present study aims to evaluate the antihyperlipidaemic, antihypertrophic, and reducing effects of zingerone on isoproterenol-induced hyperlipidaemia and hypertrophy in rats. Rats were pretreated with zingerone (6 mg/kg body weight) daily for a period of 14 days and then induced myocardial infarction with isoproterenol (100 mg/kg body weight) on days 15 and 16. Isoproterenol increased serum creatine kinase and lactate dehydrogenase activities in the rats. Increased levels/concentrations of serum and heart cholesterol and triglycerides were observed in isoproterenol-induced myocardial infarcted rats. Isoproterenol also altered serum lipoproteins and the activity of liver 3-hydroxy-3-methyl glutaryl-coenzyme-A-reductase in the rats. The in vitro study revealed a very convincing reducing power of zingerone. Pretreatment with zingerone prevented hyperlipidaemia and cardiac hypertrophy, by virtue of its antihyperlipidaemic, antihypertrophic, and reducing properties in isoproterenol-induced myocardial infarcted rats.
Asunto(s)
Cardiomegalia/prevención & control , Guayacol/análogos & derivados , Hiperlipidemias/prevención & control , Hipolipemiantes/uso terapéutico , Infarto del Miocardio/tratamiento farmacológico , Animales , Guayacol/uso terapéutico , Isoproterenol , Masculino , Infarto del Miocardio/inducido químicamente , Sustancias Protectoras/uso terapéutico , Ratas , Ratas WistarRESUMEN
The present study was aimed to evaluate the preventive effects of (-) epicatechin on alterations in the activities/levels of adenosine triphosphatases and minerals in isoproterenol-induced myocardial infarcted rats. Male albino Wistar rats were pretreated with (-) epicatechin (20 mg/kg body weight) daily for a period of 21 days. After the pretreatment period, rats were induced myocardial infarction by isoproterenol (100 mg/kg body weight) on 22nd and 23rd day. The activity of sodium/potassium-dependent adenosine triphosphatase was decreased, and the activities of calcium- and magnesium-dependent adenosine triphosphatases were increased in the heart of isoproterenol-induced myocardial infarcted rats. In addition, the concentrations of potassium were decreased and the concentrations of sodium and calcium were increased in the heart of isoproterenol-induced rats. Elevated plasma lipid peroxidation was noted in isoproterenol-induced rats. Prior treatment with (-) epicatechin significantly prevented the alterations in the activities and concentrations of adenosine triphosphatases, minerals, and plasma lipid peroxidation. The in vitro study confirmed the reducing property of (-) epicatechin. The observed effects in this study are attributed to the membrane-stabilizing and antioxidant properties of (-) epicatechin. The findings of this study will be beneficial to prevent the occurrence of myocardial infarction.
Asunto(s)
Adenosina Trifosfatasas/metabolismo , Catequina/farmacología , Isoproterenol/farmacología , Minerales/metabolismo , Infarto del Miocardio/metabolismo , Animales , Peroxidación de Lípido , Masculino , Infarto del Miocardio/inducido químicamente , Ratas , Ratas WistarRESUMEN
The present study was aimed to evaluate the protective effects of N-acetyl cysteine (NAC) on changes in the activities/levels of adenosine triphosphatases and minerals in isoproterenol-induced myocardial-infarcted rats. Male albino Wistar rats were pretreated with NAC (10 mg/kg body weight) daily for a period of 14 days. After pretreatment period, rats were induced myocardial infarction (MI) by isoproterenol (100 mg/kg body weight). The activity of sodium/potassium-dependent adenosine triphosphatase was decreased, and the activities of calcium- and magnesium-dependent adenosine triphosphatases were increased in the heart of isoproterenol-induced myocardial-infarcted rats. Furthermore, the levels of potassium were lowered and the levels of sodium and calcium were increased in the heart of isoproterenol-induced rats. Increased plasma lipid peroxidation was observed in isoproterenol-induced rats. Pretreatment with NAC showed protective effects on adenosine triphosphatases, minerals, and lipid peroxidation. The in vitro study confirmed the reducing property of NAC. The observed effects are due to the membrane-stabilizing and antioxidant effects of NAC. The results of this study will be useful for the prevention of MI.
Asunto(s)
Acetilcisteína/farmacología , Adenosina Trifosfatasas/sangre , Cardiotónicos/farmacología , Proteínas de la Membrana/sangre , Infarto del Miocardio/sangre , Animales , Membrana Celular/enzimología , Activación Enzimática/efectos de los fármacos , Isoproterenol/toxicidad , Peroxidación de Lípido/efectos de los fármacos , Masculino , Minerales/sangre , Terapia Molecular Dirigida , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/tratamiento farmacológico , Ratas , Ratas Wistar , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
The growing burden of myocardial infarction (MI) becomes a major global health issue that is accountable for considerable mortality worldwide. Hence, it is obligatory to develop a new treatment for MI having lesser side effects. Cardiac hypertrophy, oxidative stress, and inflammatory pathways play crucial roles in the pathogenesis of MI. This investigation established the anti-cardiac hypertrophic, antioxidant, anti-inflammatory, and myocardial infarct size limiting effects of valencene. Rats were induced MI by isoproterenol (100 mg/kg body weight) and then treated with valencene and cardiac sensitive markers, cardiac hypertrophy, oxidative stress, markers of inflammation, nuclear factor- κB inflammatory pathway, and myocardial infarct size was estimated/determined. The serum cardiac diagnostic markers, cardiac hypertrophy, conjugated dienes, markers of inflammation, pro-inflammatory cytokines, and myocardial infarct size were significantly (P < 0.05) increased by isoproterenol. Further, antioxidant enzymes and anti-inflammatory cytokine gene were significantly (P < 0.05) decreased in the heart. The 2, 3, 5-triphenyl tetrazolium chloride dye staining revealed a larger infarct size. Moreover, histological results of myocardial infarcted rat's cardiac tissue revealed separation of cardiac muscle fibers, necrosis, and inflammatory cells. Post-treatment with valencene (12 mg/kg body weight) orally, daily, for two weeks to isoproterenol-induced myocardial infarcted rats reversed all above said structural, biochemical, molecular, and histological parameters investigated, by its anti-cardiac hypertrophic, antioxidant, anti-inflammatory, and myocardial infarct size limiting effects. Thus, valencene is a potential candidate for inhibiting cardiac hypertrophy, oxidative stress, nuclear factor- κB inflammatory pathway, and myocardial infarct size and exhibited cardioprotection in MI.
Asunto(s)
Antioxidantes , Infarto del Miocardio , Animales , Antiinflamatorios , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Peso Corporal , Cardiomegalia/inducido químicamente , Cardiomegalia/tratamiento farmacológico , Cardiomegalia/metabolismo , Inflamación/metabolismo , Isoproterenol/farmacología , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/tratamiento farmacológico , Infarto del Miocardio/metabolismo , Miocardio/metabolismo , Miocitos Cardíacos/metabolismo , FN-kappa B/metabolismo , Estrés Oxidativo , Ratas , Ratas Wistar , SesquiterpenosRESUMEN
The present study aims to evaluate the combined protective effects of quercetin and α-tocopherol on isoproterenol-treated myocardial infarcted rats. Male albino Wistar rats were pretreated with a combination of quercetin (10 mg/kg) and α-tocopherol (10 mg/kg) daily for 14 days. After the pretreatment, rats were injected isoproterenol (100 mg/kg) to induce myocardial infarction. Isoproterenol-treated rats showed increased levels of serum troponins and increased intensities of serum lactate dehydrogenase-1 and -2 isoenzyme bands. Isoproterenol treatment also showed significant decreased levels of antioxidant system and significant increased levels of plasma lipid peroxidation, plasma uric acid, and the heart calcium. Furthermore, isoproterenol-treated rat's electrocardiogram showed elevated ST segments. Combined pretreatment with quercetin and α-tocopherol normalized all the biochemical parameters and minimized the alterations in electrocardiogram. Histopathology of myocardium also confirmed the cardioprotective effects of quercetin and α-tocopherol. In vitro studies confirmed the mechanism of action of quercetin and α-tocopherol. Thus, quercetin and α-tocopherol exhibited cardioprotective effects against isoproterenol-induced cardiotoxicity due to their scavenging free radicals, improving antioxidants and maintaining Ca(2+) levels. Our study also showed that combined pretreatment (quercetin and α-tocopherol) was highly effective than single pretreatment (quercetin or α-tocopherol).
Asunto(s)
Antioxidantes/metabolismo , Calcio/metabolismo , Cardiotónicos/farmacología , Infarto del Miocardio/tratamiento farmacológico , Miocardio/metabolismo , Quercetina/farmacología , alfa-Tocoferol/farmacología , Animales , Ácido Ascórbico/metabolismo , Quimioterapia Combinada , Electrocardiografía/métodos , Depuradores de Radicales Libres/farmacología , Glutatión/efectos de los fármacos , Glutatión/metabolismo , Glutatión Peroxidasa/efectos de los fármacos , Glutatión Peroxidasa/metabolismo , Glutatión Reductasa/efectos de los fármacos , Glutatión Reductasa/metabolismo , Isoenzimas/efectos de los fármacos , Isoenzimas/metabolismo , Isoproterenol , L-Lactato Deshidrogenasa/efectos de los fármacos , L-Lactato Deshidrogenasa/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Masculino , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/metabolismo , Miocardio/citología , Miocardio/patología , Ratas , Ratas Wistar , Superóxido Dismutasa/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Troponina I/sangre , Troponina I/efectos de los fármacos , Troponina I/metabolismo , Troponina T/sangre , Troponina T/efectos de los fármacos , Troponina T/metabolismo , Ácido Úrico/sangre , Ácido Úrico/metabolismoRESUMEN
The present study aims to evaluate the protective effects of caffeic acid on isoproterenol-treated myocardial infarction. Male albino Wistar rats were pretreated with caffeic acid (15 mg/kg) daily for 10 days. After the pretreatment, rats were injected with isoproterenol (100 mg/kg) at an interval of 24 h for 2 days to induce myocardial infarction. Isoproterenol-treated rats showed increased intensity of lactate dehydrogenase-1 and 2 isoenzyme bands and elevated ST segments. The activity of the heart sodium potassium adenosine triphosphatase was decreased, and the activities of the heart magnesium adenosine triphosphatase and calcium adenosine triphosphatase were increased in isoproterenol-treated rats. Isoproterenol-treated rats also showed a significant increase in the concentration of heart calcium. Furthermore, it significantly increased the counts of red blood cells, hemoglobin, white blood cells, and neutrophils and decreased significantly the concentration of erythrocyte sedimentation rate and the counts of lymphocytes and eosinophils. Pretreatment with caffeic acid showed protective effects on all the biochemical parameters, hematology and minimized alterations in lactate dehydrogenase isoenzymes and electrocardiogram. In vitro study confirmed the free radical scavenging potential of caffeic acid. The observed effects might be due to the free radical scavenging and membrane-stabilizing property of caffeic acid.
Asunto(s)
Adenosina Trifosfatasas/metabolismo , Ácidos Cafeicos/farmacología , Depuradores de Radicales Libres/farmacología , Isoproterenol/toxicidad , L-Lactato Deshidrogenasa/metabolismo , Infarto del Miocardio/tratamiento farmacológico , Animales , Evaluación Preclínica de Medicamentos , Electrocardiografía , Hematología/métodos , Isoenzimas/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Masculino , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/fisiopatología , Ratas , Ratas WistarRESUMEN
This study evaluated the protective effects of gallic acid on brain lipid peroxidation products, antioxidant system, and lipids in streptozotocin-induced type II diabetes mellitus. Streptozotocin-induced diabetic rats showed a significant increase in the levels of blood glucose, brain lipid peroxidation products, and lipids and a significant decrease in the activities of brain enzymic antioxidants. Oral treatment with gallic acid (10 mg and 20 mg/kg) for 21 days significantly decreased the levels of blood glucose, brain lipid peroxidation products, and lipids and significantly increased the activities of brain enzymic antioxidants in diabetic rats. Histopathology of brain confirmed the protective effects of gallic acid. Furthermore, in vitro study revealed the free radical scavenging action of gallic acid. Thus, our study shows the beneficial effects of gallic acid on brain metabolism in streptozotocin-induced type II diabetic rats. A diet containing gallic acid may be beneficial to type II diabetic patients.
Asunto(s)
Antioxidantes/farmacología , Diabetes Mellitus Experimental/tratamiento farmacológico , Ácido Gálico/farmacología , Metabolismo de los Lípidos/efectos de los fármacos , Peróxidos Lipídicos/metabolismo , Análisis de Varianza , Animales , Compuestos de Bifenilo/metabolismo , Glucemia/análisis , Encéfalo/patología , Diabetes Mellitus Experimental/fisiopatología , Peroxidación de Lípido , Lípidos/sangre , Masculino , Picratos/metabolismo , Ratas , Ratas Wistar , Estreptozocina/toxicidad , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
We evaluated the protective effects of gallic acid (3,4,5-trihydroxybenzoic acid) on hepatic lipid peroxidation products, antioxidants, glycoprotein components, and lipids in streptozotocin-induced type II diabetic rats. To induce type II diabetes, rats were injected with streptozotocin intraperitoneally at a single dose of 40 mg/kg. Gallic acid (10 and 20 mg/kg) treatment was given to diabetic rats orally using an intragastric tube daily for 21 days. Streptozotocin-induced diabetic rats showed a significant increase in the levels of blood glucose, hepatic lipid peroxidation products, glycoprotein components, lipids, and the activity of HMG-CoA reductase and a significant decrease in the levels of plasma insulin and liver glycogen. In addition to this, the activities/levels of hepatic antioxidants were decreased in diabetic rats. Gallic acid (10 and 20 mg/kg) treatment showed significant protective effects on all the biochemical parameters studied in diabetic rats. Thus, our study shows the antihyperglycemic, antilipid peroxidative, antioxidant, and antilipidemic effects of gallic acid in streptozotocin-induced type II diabetic rats. A diet containing gallic acid may be beneficial to type II diabetic patients.
Asunto(s)
Antioxidantes/uso terapéutico , Diabetes Mellitus Tipo 2/tratamiento farmacológico , Ácido Gálico/uso terapéutico , Glicoproteínas/sangre , Hipoglucemiantes/uso terapéutico , Peroxidación de Lípido/efectos de los fármacos , Análisis de Varianza , Animales , Glucemia/análisis , Diabetes Mellitus Experimental/tratamiento farmacológico , Diabetes Mellitus Experimental/patología , Diabetes Mellitus Tipo 2/inducido químicamente , Diabetes Mellitus Tipo 2/patología , Glicoproteínas/metabolismo , Peróxidos Lipídicos/sangre , Glucógeno Hepático/análisis , Masculino , Ratas , Ratas Wistar , Estreptozocina , Triglicéridos/sangreRESUMEN
Mitochondrial dysfunction plays an important role in the pathology of myocardial infarction. We evaluated the combined protective effects of quercetin and α-tocopherol on mitochondrial damage and myocardial infarct size in isoproterenol-induced myocardia- infarcted rats. Rats were pretreated with quercetin (10 mg/kg) alone, α-tocopherol (10 mg/kg) alone, and combination of quercetin (10 mg/kg) and α-tocopherol (10 mg/kg) orally using an intragastric tube daily for 14 days. After pretreatment, rats were induced myocardial infarction by isoproterenol (100 mg/kg) at an interval of 24 h for 2 days. Isoproterenol treatment caused significant increase in mitochondrial lipid peroxides with significant decrease in mitochondrial antioxidants. Significant decrease in the activities of isocitrate, succinate, malate, and α-ketoglutarate and NADH dehydrogenases and cytochrome-c-oxidase, significant increase in calcium, and significant decrease in adenosine triphosphate were observed in mitochondria of myocardial infarcted rats. Combined pretreatment with quercetin and α-tocopherol normalized all the biochemical parameters and preserved the integrity of heart tissue and restored normal mitochondrial function in myocardial-infarcted rats. Transmission electron microscopic findings on heart mitochondria and macroscopic enzyme mapping assay on the size of myocardial infarct also correlated with these biochemical parameters. The present study showed that combined pretreatment was highly effective than single pretreatment.
Asunto(s)
Mitocondrias Cardíacas/metabolismo , Infarto del Miocardio/patología , Quercetina/farmacología , alfa-Tocoferol/farmacología , Adenosina Trifosfato/metabolismo , Animales , Antioxidantes/metabolismo , Calcio/metabolismo , Sinergismo Farmacológico , Complejo IV de Transporte de Electrones/metabolismo , Isocitratos/metabolismo , Isoproterenol , Ácidos Cetoglutáricos/metabolismo , Peróxidos Lipídicos/metabolismo , Malatos/metabolismo , Masculino , Microscopía Electrónica de Transmisión , Mitocondrias Cardíacas/efectos de los fármacos , Mitocondrias Cardíacas/enzimología , Mitocondrias Cardíacas/ultraestructura , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/enzimología , Miocardio/enzimología , Miocardio/patología , NADH Deshidrogenasa/metabolismo , Sustancias Protectoras/farmacología , Distribución Aleatoria , Ratas , Ratas Wistar , Succinatos/metabolismo , Factores de Tiempo , Pruebas de Toxicidad/métodosRESUMEN
This article reports data on the preventive effect of (-)epigallocatechin gallate (EGCG) on lipid metabolism and lipoproteins in isoproterenol (ISO)-induced myocardial infarction (MI) in Wistar rats. The rats were induced MI by ISO (100 mg/kg) at an interval of 24 h for 2 days. EGCG (30 mg/kg) was given to rats as pretreatment for 21 days orally using an intragastric tube. EGCG significantly reduced the increased serum levels of cholesterol, triglycerides, and free fatty acids in the heart and serum phospholipids (PLs) in ISO-treated rats. It also significantly increased the reduced levels of heart PLs in ISO-induced rats. EGCG reduced the levels of serum low-density lipoprotein cholesterol and very low-density lipoprotein cholesterol and increased serum high-density lipoprotein (HDL)-cholesterol in ISO-treated rats. It also reduced the increased cholesterol/PL ratio and atherogenic index and significantly increased the reduced ratio of HDL-cholesterol/total cholesterol. Also EGCG significantly increased the reduced activity of lecithin cholesterol acyl transferase in ISO-treated rats. Thus, EGCG prevented the accumulation of lipids and altered the levels of lipoproteins in myocardial-infarcted rats.
Asunto(s)
Catequina/análogos & derivados , Isoproterenol/farmacología , Metabolismo de los Lípidos/efectos de los fármacos , Lipoproteínas/metabolismo , Infarto del Miocardio/metabolismo , Animales , Catequina/farmacología , LDL-Colesterol/sangre , VLDL-Colesterol/sangre , Masculino , Ratas , Ratas WistarRESUMEN
Cardiac mitochondrial oxidative stress causes mitochondrial damage that plays an important role in the pathology of myocardial infarction. The preventive effects of diosmin on cardiac mitochondrial oxidative stress in isoproterenol-induced myocardial infarcted rats were evaluated. Rats were pretreated with diosmin (10 mg/kg body weight) daily for 10 days. Myocardial infarction was induced in rats by isoproterenol (100 mg/kg body weight) injection twice at an interval of 24 h (on 11th and 12th day). Isoproterenol-induced myocardial infarcted rats showed a significant increase in the levels of cardiac diagnostic markers, heart mitochondrial lipid peroxidation, calcium ion, and a significant decrease in the levels of heart mitochondrial glutathione peroxidase, reduced glutathione, glutathione-S-transferase, isocitrate, malate, α-ketoglutarate, and succinate dehydrogenases. Transmission electron microscopic findings revealed damaged mitochondria with loss of cristae, swelling, and vacuolation in isoproterenol-induced rats' heart. Diosmin pretreatment showed significant preventive effects on all the biochemical parameters, and the structure of mitochondria was evaluated. Furthermore, the transmission electron microscopic study confirms the biochemical findings. The antioxidant and negative inotropic effects of diosmin inhibited cardiac mitochondrial oxidative stress and prevented mitochondrial damage in myocardial infarcted rats.
Asunto(s)
Antioxidantes/farmacología , Diosmina/farmacología , Isoproterenol , Mitocondrias Cardíacas/efectos de los fármacos , Infarto del Miocardio/tratamiento farmacológico , Miocitos Cardíacos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Animales , Biomarcadores/metabolismo , Señalización del Calcio/efectos de los fármacos , Cardiotoxicidad , Citoprotección , Modelos Animales de Enfermedad , Peroxidación de Lípido/efectos de los fármacos , Masculino , Mitocondrias Cardíacas/metabolismo , Mitocondrias Cardíacas/ultraestructura , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/metabolismo , Infarto del Miocardio/patología , Miocitos Cardíacos/metabolismo , Miocitos Cardíacos/ultraestructura , Ratas WistarRESUMEN
Diets rich in natural antioxidants are associated with reduced risk of heart diseases. This study was aimed to evaluate the preventive role of naringin on cardiac troponin T (cTnT), lactate dehydrogenase (LDH)-isoenzyme, cardiac marker enzymes, electrocardiographic (ECG)-patterns and lysosomal enzymes in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85mg/kg) at an interval of 24h for 2 days showed a significant increase in the levels of cTnT, intensity of the bands of LDH-isoenzyme (LDH1 and LDH2) and the activities of cardiac marker enzymes such as creatine kinase-MB (CK-MB), creatine kinase (CK), LDH, aspartate transaminase (AST) and alanine transaminase (ALT) in serum with subsequent decrease in the activities of CK, LDH, AST and ALT in the heart and alterations in ECG-patterns. The activities of lysosomal enzymes (beta-glucuronidase, beta-N-acetyl glucosaminidase, beta-galactosidase, cathepsin-B and cathepsin-D) were increased significantly in serum and the heart of ISO-induced rats, but the activities of beta-glucuronidase and cathepsin-D were decreased significantly in the lysosomal fraction of the heart. Pretreatment with naringin (10, 20 or 40mg/kg) daily for a period of 56 days positively altered the levels of cTnT, intensity of the bands of the LDH1 and LDH2-isoenzyme and the activities of cardiac marker enzymes, ECG-patterns and lysosomal hydrolases in ISO-induced rats. Thus, naringin possess cardioprotective effect in ISO-induced MI in rats.
Asunto(s)
Antioxidantes/farmacología , Forma MB de la Creatina-Quinasa/sangre , Flavanonas/farmacología , Infarto del Miocardio/prevención & control , Troponina T/sangre , Acetilglucosaminidasa/sangre , Alanina Transaminasa/sangre , Animales , Aspartato Aminotransferasas/sangre , Catepsina B/sangre , Catepsina D/sangre , Electrocardiografía , Glucuronidasa/sangre , Isoenzimas/sangre , Isoproterenol , L-Lactato Deshidrogenasa/sangre , Masculino , Infarto del Miocardio/sangre , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/metabolismo , Ratas , Ratas Wistar , beta-Galactosidasa/sangreRESUMEN
This study was designed to evaluate the cardioprotective potential of naringin on lipid peroxides, enzymatic and nonenzymatic antioxidants and histopathological findings in isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (85 mg/kg) to male Wistar rats showed a significant increase in the levels of thiobarbituric acid reactive substances and lipid hydroperoxides in plasma and the heart and a significant decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase in the heart and the levels of reduced glutathione, vitamin C and vitamin E in plasma and heart and ceruloplasmin in plasma. Oral administration of naringin (10, 20 and 40 mg/kg, respectively) to ISO-induced rats daily for a period of 56 days showed a significant decrease in the levels of lipid peroxidative products and improved the antioxidant status by increasing the activities of antioxidant enzymes and nonenzymatic antioxidants. Histopathological findings of the myocardial tissue showed the protective role of naringin in ISO-induced rats. The effect at a dose of 40 mg/kg of naringin was more pronounced than that of the other two doses, 10 and 20mg/kg. The results of our study show that naringin possess anti-lipoperoxidative and antioxidant activity in experimentally induced cardiac toxicity.
Asunto(s)
Agonistas Adrenérgicos beta/toxicidad , Antioxidantes/metabolismo , Antioxidantes/farmacología , Flavanonas/farmacología , Cardiopatías/inducido químicamente , Isoproterenol/toxicidad , Peróxidos Lipídicos/metabolismo , Animales , Ácido Ascórbico/metabolismo , Ceruloplasmina/metabolismo , Enzimas/metabolismo , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Glutatión Transferasa/metabolismo , Cardiopatías/metabolismo , Cardiopatías/patología , Peróxido de Hidrógeno/metabolismo , Metabolismo de los Lípidos/efectos de los fármacos , Lipoproteínas/metabolismo , Masculino , Fibras Musculares Esqueléticas/patología , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/metabolismo , Infarto del Miocardio/patología , Miocardio/metabolismo , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
We previously reported that rutin administration to streptozotocin (STZ)-induced diabetic rats decreased plasma glucose and increased plasma insulin levels. In this study, we have examined the role of rutin on matrix remodelling in the kidney of STZ-induced diabetic rats. STZ was administered intraperitoneally (50 mg kg(-1)) to male albino Wistar rats to induce experimental diabetes. Rutin (100 mg kg(-1)) was orally administered to normal and STZ-induced diabetic rats for a period of 45 days and its influence on the content of hydroxyproline and collagen and on the activity of matrix metalloproteinases (MMPs) were studied. We have also studied the levels of tissue inhibitors of metalloproteinases (TIMPs) in the kidney. STZ-induced diabetic control rats showed increased content of hydroxyproline and collagen, decreased activity of MMPs and increased levels of TIMPs in the kidney. These changes were positively modulated by rutin treatment in STZ-induced diabetic rats, thereby protecting the kidney. In normal rats treated with rutin, none of the parameters studied were significantly altered. From the results obtained, we could conclude that rutin influences MMPs and effectively protects kidney against STZ-induced damage in rats. The effects observed are due to the reduction of plasma glucose levels by rutin.
Asunto(s)
Diabetes Mellitus Experimental/metabolismo , Nefropatías Diabéticas/metabolismo , Matriz Extracelular/efectos de los fármacos , Matriz Extracelular/metabolismo , Rutina/farmacología , Animales , Glucemia/metabolismo , Colágeno/metabolismo , Diabetes Mellitus Experimental/patología , Nefropatías Diabéticas/patología , Matriz Extracelular/patología , Hidroxiprolina/metabolismo , Insulina/sangre , Riñón/metabolismo , Masculino , Metaloproteinasas de la Matriz/metabolismo , Ratas , Ratas Wistar , Inhibidores Tisulares de Metaloproteinasas/metabolismoRESUMEN
The consumption of diets rich in plant foods is associated with a reduced risk of cardiovascular diseases. This study aimed to evaluate the preventive role of rutin on lipid peroxides and antioxidants in normal and isoproterenol-induced myocardial infarction in rats. Subcutaneous injection of isoproterenol (150 mg kg(-1)) to male Wistar rats at an interval of 24 h for two days showed a significant increase in the activity of serum cardiac marker enzymes (creatine kinase, lactate dehydrogenase, aspartate transaminase and alanine transaminase) and a significant decrease in the activity of these enzymes in the heart. Lipid peroxidative products (thiobarbituricacid reactive substances and lipid hydroperoxides) were significantly increased and enzymic (superoxide dismutase, catalase and glutathione peroxidase) and non-enzymic (reduced glutathione and vitamin C) antioxidants showed a significant decrease in isoproterenol-treated rats. Pretreatment with rutin (40 or 80 mg kg(-1)) to isoproterenol-treated rats orally for a period of 42 days daily caused a significant effect. Administration of rutin to normal rats did not have any significant effect on any of the parameters studied. The results of our study show that rutin possesses antioxidant activity in isoproterenol-induced experimental myocardial infarction.
Asunto(s)
Antioxidantes/farmacología , Corazón/efectos de los fármacos , Peróxidos Lipídicos/metabolismo , Infarto del Miocardio/prevención & control , Miocardio/metabolismo , Rutina/farmacología , Animales , Ácido Ascórbico/sangre , Ácido Ascórbico/metabolismo , Aspartato Aminotransferasas/sangre , Aspartato Aminotransferasas/metabolismo , Catalasa/metabolismo , Forma MB de la Creatina-Quinasa/sangre , Forma MB de la Creatina-Quinasa/metabolismo , Relación Dosis-Respuesta a Droga , Glutatión/sangre , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Isoproterenol , L-Lactato Deshidrogenasa/sangre , L-Lactato Deshidrogenasa/metabolismo , Peróxidos Lipídicos/sangre , Masculino , Infarto del Miocardio/sangre , Infarto del Miocardio/inducido químicamente , Ratas , Superóxido Dismutasa/metabolismoRESUMEN
The protective role of rutin on lipids, lipoproteins, lipid metabolizing enzymes and glycoproteins in streptozotocin-induced diabetic rats has been studied. A single intraperitoneal injection of streptozotocin (50 mg kg(-1)) to rats led to a significant (P < 0.05) increase in the levels of lipids (cholesterol, triglycerides, free fatty acids and phospholipids) in plasma and tissues (liver, kidney, heart and brain). The levels of low density and very low density lipoprotein (LDL and VLDL, respectively) cholesterol were increased, whereas the levels of high density lipoprotein (HDL) cholesterol were decreased significantly (P < 0.05) in plasma. The activity of 3-hydroxy 3-methylglutaryl coenzyme A (HMG CoA) reductase increased significantly (P < 0.05) in liver, kidney and heart, and the activity of lipoprotein lipase (LPL) and lecithin cholesterol acyltransferase (LCAT) decreased significantly (P < 0.05) in the plasma of diabetic rats. Streptozotocin injection also increased the levels of glycoproteins such as hexose, hexosamine, fucose and sialic acid in plasma, liver and kidney. Oral administration of rutin to streptozotocin-induced diabetic rats significantly (P < 0.05) decreased the levels of lipids in plasma and tissues. The levels of plasma HDL-cholesterol increased and the levels of LDL- and VLDL-cholesterol decreased significantly (P < 0.05). The activity of HMG CoA reductase decreased in the tissues and the activity of plasma LPL and LCAT increased significantly (P < 0.05). The levels of glycoproteins were found to be significantly (P < 0.05) decreased in plasma, liver and kidney of rutin-treated diabetic rats. Rutin administration to normal rats did not exhibit any significant (P < 0.05) changes in any of the parameters studied. In conclusion, the beneficial effect of rutin on lipids, lipoproteins, lipid metabolizing enzymes and glycoproteins could be due to its antioxidant property.
Asunto(s)
Diabetes Mellitus Experimental/tratamiento farmacológico , Glicoproteínas/sangre , Metabolismo de los Lípidos/efectos de los fármacos , Lipoproteínas/sangre , Sustancias Protectoras/farmacología , Rutina/farmacología , Animales , Colesterol/sangre , Colesterol/metabolismo , Diabetes Mellitus Experimental/sangre , Diabetes Mellitus Experimental/metabolismo , Ácidos Grasos no Esterificados/sangre , Ácidos Grasos no Esterificados/metabolismo , Glicoproteínas/metabolismo , Hidroximetilglutaril-CoA Reductasas/metabolismo , Lipoproteína Lipasa/sangre , Lipoproteínas/metabolismo , Lipoproteínas HDL/sangre , Lipoproteínas HDL/metabolismo , Lipoproteínas LDL/sangre , Lipoproteínas LDL/metabolismo , Lipoproteínas VLDL/sangre , Lipoproteínas VLDL/metabolismo , Masculino , Fosfatidilcolina-Esterol O-Aciltransferasa/sangre , Fosfolípidos/sangre , Fosfolípidos/metabolismo , Ratas , Ratas Wistar , Estreptozocina , Triglicéridos/sangre , Triglicéridos/metabolismoRESUMEN
Myocardial infarction continues to be a major public health problem. Reduction in mortality rate and prevention of myocardial infarction are of utmost importance. Inflammation and thrombosis play an important role in the pathogenesis of myocardial infarction. The anti-inflammatory and anti-thrombotic effects of zingerone were evaluated in isoproterenol induced myocardial infarcted rats. Rats were pretreated with zingerone (6mg/kg body weight) daily for 14 days and were then induced myocardial infarction with isoproterenol (100mg/kg body weight) on 15th and 16th day. Isoproterenol induced myocardial infarcted rats showed significant (P<0.05) increase in the levels/ activities of cardiac troponin-I (cTnI), high sensitive C-reactive protein (Hs CRP), lysosomal hydrolases in the serum and concentration of heart lysosomal lipid peroxidation (LPO) products. RT-PCR study revealed over expression of myocardial tumour necrosis factor - alpha (TNF-α), interleukin-1ß (IL-1ß) and interleukin-6 (IL-6) genes in the myocardial infarcted rats. Histopathology of heart and coronary artery revealed marked inflammation and coronary thrombosis. Zingerone pretreatment significantly (P<0.05) decreased serum cTnI, Hs CRP, lysosomal hydrolases and heart lysosomal LPO and down regulated myocardial TNF-α, IL-1ß and IL-6 genes and prevented coronary thrombosis in isoproterenol induced myocardial infarcted rats. The observed effects of zingerone could be attributed to its anti-inflammatory and anti-thrombotic properties.
Asunto(s)
Antiinflamatorios/farmacología , Fibrinolíticos/farmacología , Guayacol/análogos & derivados , Infarto del Miocardio/tratamiento farmacológico , Animales , Antiinflamatorios/uso terapéutico , Biomarcadores/sangre , Modelos Animales de Enfermedad , Fibrinolíticos/uso terapéutico , Gliceraldehído-3-Fosfato Deshidrogenasa (Fosforilante)/genética , Guayacol/farmacología , Guayacol/uso terapéutico , Interleucina-1beta/genética , Interleucina-6/genética , Isoproterenol/farmacología , Masculino , Infarto del Miocardio/sangre , Infarto del Miocardio/inducido químicamente , Infarto del Miocardio/genética , Ratas , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
Trigonella foenum-graecum, commonly known as fenugreek, is a traditional medicinal plant of the Leguminoseae family in India. The antioxidant effect of fenugreek leaves was evaluated in the streptozotocin-induced diabetic rat model. The antioxidant effect was evaluated by estimating thiobarbituric acid-reactive substances and reduced glutathione and measuring the activities of catalase and superoxide dismutase in liver, heart, and kidney in diabetic rats. Fenugreek leaf powder supplementation significantly lowered lipid peroxidation and significantly increased the antioxidant system in diabetic rats. The effect at a dose of 1 g/kg of body weight of fenugreek leaf powder was similar to that of glibenclamide. Insulin restores all the parameters to near normal values. Thus, fenugreek leaf powder reduces oxidative stress in experimental diabetes.
Asunto(s)
Diabetes Mellitus Experimental/terapia , Estrés Oxidativo , Hojas de la Planta/química , Trigonella/química , Animales , Antioxidantes/análisis , Antioxidantes/farmacología , Catalasa/análisis , Diabetes Mellitus Experimental/enzimología , Diabetes Mellitus Experimental/metabolismo , Suplementos Dietéticos , Femenino , Glutatión/análisis , Glutatión/química , Gliburida/administración & dosificación , Riñón/enzimología , Peroxidación de Lípido , Hígado/enzimología , Miocardio/enzimología , Oxidación-Reducción , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas Wistar , Superóxido Dismutasa/análisis , Sustancias Reactivas al Ácido Tiobarbitúrico/análisisRESUMEN
Hyponidd is a herbomineral formulation composed of the extracts of ten medicinal plants ( Momordica charantia, Melia azadirachta, Pterocarpus marsupium, Tinospora cordifolia , Gymnema sylvestre, Enicostemma littorale, Emblica officinalis, Eugenia jambolana, Cassia auriculata and Curcuma longa). We have investigated hyponidd for its possible antihyperglycaemic and antioxidant effect in diabetic rats. Rats were rendered diabetic by streptozotocin (STZ) (45 mg kg(-1) body weight). Oral administration of hyponidd (100 mg kg(-1) and 200 mg kg(-1)) for 45 days resulted in significant lowered levels of blood glucose and significant increased levels of hepatic glycogen and total haemoglobin. An oral glucose tolerance test was also performed in experimental diabetic rats in which there was a significant improvement in blood glucose tolerance in the rats treated with hyponidd. Hyponidd administration also decreased levels of glycosylated haemoglobin, plasma thiobarbituric acid reactive substances, hydroperoxides, ceruloplasmin and alpha-tocopherol in diabetic rats. Plasma reduced glutathione and vitamin C were significantly elevated by oral administration of hyponidd. The effect of hyponidd at a dose of 200 mg kg(-1) was more effective than glibenclamide (600 microg kg(-1)) in restoring the values to near normal. The results showed that hyponidd exhibits antihyperglycaemic and antioxidant activity in STZ-induced diabetic rats.