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1.
Zhongguo Zhong Yao Za Zhi ; 39(2): 291-5, 2014 Jan.
Artículo en Zh | MEDLINE | ID: mdl-24761648

RESUMEN

OBJECTIVE: To observe the effect and mechanism of Dendrobium candidum polysaccharides (DCP) in promoting hair growth, in order to lay a foundation for the development and utilization of D. candidum. METHOD: The water-extraction and alcohol-precipitation method was adopted to extract DCP, and the phenol-sulphuric acid method was used to determine its content. Thirty C57BL6J mice were collected to establish the hair loss model with hair removal cream. They were randomly divided into the control group, the positive control group and the DCP group, and given 0.2 mL of ultra-pure water, minoxidil tincture and DCP (5.0 g x L(-1)) 21 days. The mice hair growth scoring standard was adopted to evaluate the hair growth of C57BL/6J mice at 7, 14 d. The hairs in unit hair-losing areas of treated C57BL/6J mice at 21 d were weighed to evaluate the effect of DCP on the promotion of hair growth. MTT assay and RT-PCR method were used to evaluate the effect of DCP on the proliferatin of HaCaT cells and the mRNA expression of VEGF in HaCaT cells. RESULT: The extraction percent of DCP was 29.87%, and its content was 79.65%. The average scores for the hair growth and weight of C57BL/6J mice of DCP group were much higher than the control group. The survival rate and mRNA expression of VEGF of HaCaT cells were much higher than the control group. CONCLUSION: DCP has the effect in promoting hair growth. Its mechanism may be related to the up-regulation of the mRNA expression of VEGF.


Asunto(s)
Dendrobium/química , Cabello/efectos de los fármacos , Cabello/crecimiento & desarrollo , Polisacáridos/farmacología , Animales , Línea Celular , Proliferación Celular/efectos de los fármacos , Femenino , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Ratones , Ratones Endogámicos C57BL , ARN Mensajero/genética , ARN Mensajero/metabolismo , Factor A de Crecimiento Endotelial Vascular/genética
2.
J Chin Med Assoc ; 81(12): 1052-1059, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-30143430

RESUMEN

BACKGROUND: Previous studies have revealed that quantitative hepatitis B surface antigen (HBsAg) or hepatitis B core antibody (qAnti-HBc) levels can be used as predictors of treatment response in both interferon-α and nucleoside analogue therapies. Few data have been published regarding the relationship between quantitative HBsAg or Anti-HBc levels and liver fibrosis stages in patients with chronic hepatitis B (CHB). METHODS: We conducted a cross-sectional study of treatment-naïve CHB patients. A total of 624 CHB patients were recruited. We assessed the serum HBsAg and qAnti-HBc levels, HBV DNA levels, HBV genotypes, BCP/PC mutations, histological fibrosis staging by Scheuer classification. RESULTS: In HBeAg (+) patients, the S0-1 subjects had significantly higher serum HBsAg and lower qAnti-HBc levels than the S2-4 subjects (both p < 0.001). A moderate inverse correlation was present between serum HBsAg levels and fibrosis scores (r = -0.381, p < 0.001), and a moderate positive correlation was found between qAnti-HBc levels and fibrosis scores (r = 0.408, p < 0.001). In the HBeAg (-) patients, the S0-1 subjects also had significantly lower qAnti-HBc levels than the S2-4 subjects (p < 0.001); however, no significant difference in the HBsAg levels was observed between the S0-1 and S2-4 subjects (p > 0.05). Serum qAnti-HBc levels showed a moderate positive correlation with fibrosis scores (r = 0.383, p < 0.001), while serum HBsAg levels exhibited a low inverse correlation with fibrosis scores (r = -0.171, p < 0.001). Multiple logistic regression analysis showed that the parameters for predicting significant fibrosis (S ≥ 2) included age, PLT, qAnti-HBc levels, HBV genotype and BCP/PC mutations in HBeAg (+) group, and age, PLT, qAnti-HBc levels in HBeAg (-) group (all p < 0.05). The AUC of qAnti-HBc levels associated with the diagnosis of significant fibrosis abnormalities in HBeAg (+) and HBeAg (-) patients were 0.734 (95%CI 0.689 to 0.778) and 0.707 (95%CI 0.612 to 0.801), respectively. CONCLUSION: Our study found an association between high serum qAnti-HBc levels and significant fibrosis in both HBeAg (+) and HBeAg (-) treatment-naïve CHB patients. However, low serum HBsAg levels were correlated with moderate to severe fibrosis in HBeAg (+) subjects only.


Asunto(s)
Anticuerpos contra la Hepatitis B/sangre , Antígenos del Núcleo de la Hepatitis B/inmunología , Antígenos de Superficie de la Hepatitis B/sangre , Hepatitis B Crónica/complicaciones , Cirrosis Hepática/etiología , Adulto , Estudios Transversales , Femenino , Virus de la Hepatitis B/clasificación , Virus de la Hepatitis B/genética , Humanos , Hígado/patología , Cirrosis Hepática/microbiología , Cirrosis Hepática/patología , Modelos Logísticos , Masculino , Persona de Mediana Edad
3.
Chemosphere ; 147: 25-35, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26757132

RESUMEN

Occurrence and distribution of 15 antibiotics belonging to families of sulfonamides, fluoroquinolones, tetracyclines and chloramphenicols were investigated in water and sediment in Gaoqiao mangrove area, China, using LC-MS-MS. The influence of tidal level and mangrove vegetation on antibiotic residues were examined. The levels of antibiotics were found to be ranged from 0.15 to 198 ng L(-1) in water and from 0.08 to 849 µg kg(-1) in sediment. No significant difference in concentrations of 15 different antibiotics from water and sediment samples was observed among the high, middle and low intertidal channel. The residues of SMZ, SMTZ, OFL, NOR, ENR, OXY and FLO were significantly higher in Aegiceras corniculatum assemblage than in Avicennia marina assemblage. Although no significant difference in tested antibiotics was found between the surface and bottom sediment, mangrove vegetation can to some extent reduce the accumulation for SMZ, SMTZ, OFL, NOR, CIP, OXY and TET in sediments relative to corresponding bare mudflats, implying that the environmental pollution from antibiotics may be mitigated by mangrove vegetation. Principal components analysis revealed that the terrestrial input and different habitats directly influenced the occurrence and distribution of antibiotics.


Asunto(s)
Antibacterianos/análisis , Monitoreo del Ambiente/métodos , Agua Dulce/química , Sedimentos Geológicos/química , Contaminación Química del Agua/análisis , Humedales , Avicennia/química , Avicennia/crecimiento & desarrollo , China , Ecosistema , Primulaceae/química , Primulaceae/crecimiento & desarrollo
4.
Medicine (Baltimore) ; 95(34): e4422, 2016 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-27559949

RESUMEN

Previous studies have shown that hepatitis B core antibody (anti-HBc) levels vary during different phases of disease in treatment-naïve chronic hepatitis B (CHB) patients and can be used as a predictor of both interferon-α and nucleoside analogue therapy response. However, there is no information on the association between the quantitative serum anti-HBc (qAnti-HBc) level and liver inflammation in CHB patients. Therefore, we investigated these relationships in a large cohort of treatment-naïve CHB patients. A total of 624 treatment-naïve CHB patients were included in the study. The serum qAnti-HBc level was moderately correlated with ALT and AST levels (P < 0.001) in both hepatitis B e antigen-positive (HBeAg [+]) and HBeAg-negative (HBeAg [-]) CHB patients. CHB patients with no to mild inflammation (G0-1) had significantly lower serum qAnti-HBc levels than patients with moderate to severe inflammation (G2-4) (P < 0.001). Receiver operating characteristic analysis suggested that a serum qAnti-HBc cut-off value of 4.36 log10 IU/mL provided a sensitivity of 71.68%, specificity of 73.81%, positive predictive value of 78.43%, and negative predictive value of 66.24% in HBeAg (+) CHB patients with moderate to severe inflammation (G≥2). A cut-off value of 4.62 log10 IU/mL provided a sensitivity of 54.29%, specificity of 90.00%, positive predictive value of 95.00%, and negative predictive value of 36.00% in HBeAg (-) CHB patients with moderate to severe inflammation (G≥2). Serum qAnti-HBc levels were positively associated with liver inflammation grade. Furthermore, we identified optimal serum qAnti-HBc cut-off values for the prediction of inflammation activity in both HBeAg (+) and HBeAg (-) treatment-naïve CHB patients.


Asunto(s)
Anticuerpos contra la Hepatitis B/sangre , Antígenos del Núcleo de la Hepatitis B/inmunología , Hepatitis B Crónica/sangre , Hepatitis B Crónica/patología , Índice de Severidad de la Enfermedad , Adulto , Alanina Transaminasa/sangre , Aspartato Aminotransferasas/sangre , Femenino , Genotipo , Antígenos e de la Hepatitis B/sangre , Virus de la Hepatitis B/genética , Hepatitis B Crónica/virología , Humanos , Masculino , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Curva ROC , Adulto Joven
5.
Mar Pollut Bull ; 81(1): 210-7, 2014 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-24635985

RESUMEN

The effects of a thermal discharge from a coastal power plant on phytoplankton were determined in Zhanjiang Bay. Monthly cruises were undertaken at four tide times during April-October 2011. There were significant differences for dominant species among seven sampling months and four sampling tides. Species diversity (H') and Evenness showed a distinct increasing gradient from the heated water source to the control zone and fluctuated during four tides with no visible patterns. Species richness, cell count and Chl a at mixed and control zones were significantly higher than heated zones, and showed tidal changes with no obvious patterns. The threshold temperature of phytoplankton species can be regarded as that of phytoplankton community at ebb slack. The average threshold temperature over phytoplankton species, cell count and Chl a, and the threshold temperature of cell count can be regarded as that of phytoplankton community at flood slack during spring and neap respectively.


Asunto(s)
Bahías , Fitoplancton/clasificación , Centrales Eléctricas , Estaciones del Año , China , Calor , Fitoplancton/fisiología , Olas de Marea , Factores de Tiempo
6.
Mar Pollut Bull ; 62(11): 2299-307, 2011 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-21945557

RESUMEN

The resistance of bacteria to 12 different antibiotics was investigated in shrimp farms on Donghai Island, China. Antibiotic-resistant bacteria were found to be widespread in shrimp farms, indicating a high environmental risk. Further, significant differences were found in bacterial strains among farms (ANOVA, p<0.05), showing resistance to antibiotics such as ampicillin, trimethoprim, compound sinomi, tetracycline, chloramphenicol and cefazolin. No significant differences in antibiotic resistance were found among 6 hatcheries evaluated in this study (ANOVA, p>0.05), between exalted and traditional shrimp ponds (ANOVA, p>0.05), and between cultural ponds and corresponding control water source sites (T-test, p>0.05). In cultural ponds, no significant difference in bacterial resistance to antibiotics was found between water and sediment (T-test, p>0.05), and antibiotic resistance of bacteria from water showed a significant positive correlation with that from sediment (p<0.05). Therefore, our study indicates that bacterial multiple antibiotic resistance (MAR) is more widespread in shrimp hatcheries than ponds.


Asunto(s)
Antibacterianos/farmacología , Acuicultura , Bacterias/efectos de los fármacos , Farmacorresistencia Bacteriana Múltiple , Penaeidae/microbiología , Agua de Mar/microbiología , Análisis de Varianza , Animales , China , Pruebas Antimicrobianas de Difusión por Disco
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