Soil properties predict below-ground community structure, but not nematode microbiome patterns in semi-arid habitats.

Microbial and microeukaryotic communities are extremely abundant and diverse in soil habitats where they play critical roles in ecosystem functioning and services that are essential to soil health. Soil biodiversity is influenced by above-ground (vegetation) and below-ground factors (soil properties), which together create habitat-specific conditions. However, the compound effects of vegetation and soil properties on soil communities are less studied or often focused on one component of the soil biota. Here, we integrate metabarcoding (16S and 18S rRNA genes) and nematode morphology to assess the effects of habitat and soil properties shaping microbial and microeukaryotic communities as well as nematode-associated microbiomes. We show that both vegetation and soil properties (soil bulk density) were major factors structuring microbial and microeukaryotic communities in semi-arid soil habitats. Despite having lower nutrients and lower pH, denser soils displayed significantly higher alpha diversity than less dense soils across datasets. Nematode-associated microbiomes have lower microbial diversity, strongly differ from soil microbes and are more likely to respond to microscale variations among samples than to vegetation or soil bulk density. Consequently, different nematode lineages and trophic groups are likely to display similar associated microbiomes when sharing the same microhabitat. Different microbiome taxa were enriched within specific nematode lineages (e.g. Mycobacterium, Candidatus Cardinium) highlighting potentially new species-specific associations that may confer benefits to their soil nematode hosts. Our findings highlight the importance of exploring above- and below-ground effects to assess community structure in terrestrial habitats, and how fine-scale analyses are critical for understanding patterns of host-associated microbiomes.

Heparin-Bonded Expanded Polytetrafluoroethylene Is a Solution for Infrapopliteal Revascularization in the Absence of an Adequate Autologous Vein Graft.

BACKGROUND: To analyze the outcomes of heparin-bonded expanded polytetrafluoroethylene (HePTFE) graft as an alternative conduit in infrapopliteal revascularization of chronic limb-threatening ischemia (CLTI) in the absence of an autologous vein conduit. METHODS: A single-center retrospective analysis of patients with CLTI submitted to infrapopliteal bypasses with autologous vein graft (VEIN group) or HePTFE graft (HePTFE group) was implemented. Primary end points were freedom from CLTI at 12 months and recurrence of CLTI at 3 years. Secondary end points included freedom from major amputation, amputation-free survival (AFS), survival, and primary (PP) and secondary patency (SP) rates at 3 years of follow-up. RESULTS: A total of 348 limbs submitted to infrapopliteal bypasses, 214 with venous graft and 134 with HePTFE graft, were followed-up for a median of 25 months. Most patients of the HePTFE group were male (69%), with a median age of 76 years (interquartile range [IQR] 15). Fifty-nine percent of the limbs of the HePTFE group had Wound grade ≥2, being 46% of them infected. Eighty-eight percent were GLASS stage III. Freedom from CLTI was not significantly different between HePTFE and VEIN groups (75% vs. 84%, adjusted hazard ratio [aHR] 0.88, confidence interval [CI] 0.66-1.18, P = 0.401). Recurrence of CLTI was higher in the HePTFE group (42% vs.18% at 3 years; aHR 2.82, CI 1.59-5.00, P < 0.001). The VEIN group achieved higher rates of freedom from major amputation (87% vs.69% at 3 years; aHR 2.21, CI 1.31-3.75, P = 0.003) and AFS (59% vs. 37% at 3 years; aHR 1.39, CI 1.02-1.88, P = 0.036), but no significance in survival (aHR 1.10, CI 0.72-1.66, P = 0.667). Patency rates were inferior in the HePTFE group, with 2-year PP and SP rates of 52% vs. 74%, and 76% vs. 90%, respectively (PP: aHR 1.70, CI 1.11-2.59, P = 0.014; SP: aHR 2.51, CI 1.42-4.42, P = 0.001). CONCLUSIONS: Infrapopliteal bypass with autologous vein graft is the gold standard to treat CLTI limbs. HePTFE graft should be regarded as an alternative for complex infrapopliteal revascularization when lacking an autologous vein conduct.

Treatment of azo dye-containing wastewater in a combined UASB-EMBR system: Performance evaluation and membrane fouling study.

This work investigated the treatment of azo dye-containing wastewater in an upflow anaerobic sludge blanket (UASB) reactor combined with an electro-membrane bioreactor (EMBR). Current densities of 20 A m-2 and electric current exposure mode of 6'ON/30'OFF were applied to compare the performance of the EMBR to a conventional membrane bioreactor (MBR). The results showed that dye (Drimaren Red CL-7B) removal occurred predominantly in the UASB reactor, which accounted for 57% of the total dye removal achieved by the combined system. When the MBR was assisted by electrocoagulation, the overall azo dye removal efficiency increased from 60.5 to 67.1%. Electrocoagulation batch tests revealed that higher decolorization rates could be obtained with a current density of 50 A m-2. Over the entire experimental period, the combined UASB-EMBR system exhibited excellent performance in terms of chemical oxygen demand (COD) and NH4+-N removal, with average efficiencies above 97%, while PO43--P was only consistently removed when the electrocoagulation was used. Likewise, a consistent reduction in the absorption spectrum of aromatic amines was observed when the MBR was electrochemically assisted. In addition to improving the pollutants removal, the use of electrocoagulation reduced the membrane fouling rate by 68% (0.25-0.08 kPa d-1), while requiring additional energy consumption and operational costs of 1.12 kWh m-3 and 0.32 USD m-3, respectively. Based on the results, it can be concluded that the combined UASB-EMBR system emerges as a promising technological approach for textile wastewater treatment.

Infrapopliteal Bypass In Patients On Dialysis: Patency And Survival.

INTRODUCTION: Portugal has one of the highest prevalence of patients on a regular dialysis program. This population has a higher incidence of peripheral arterial disease with higher rates of postoperative morbidity and mortality. Our goal was to compare outcomes between dialysis and non-dialysis patients with chronic limb threatening ischemia (CLTI) submitted to infrapopliteal bypass. MATERIALS AND METHODS: A retrospective single-center study of infrapopliteal bypass for CLTI was performed between 2012 and 2019. Patients were divided in two groups based on dialysis status (group 1 incorporated patients on dialysis). Primary end point was 1-year freedom from CLTI. Secondary end points were limb-salvage, survival and primary (PP) and tertiary patency (TP) rates at 3 years of follow-up. RESULTS: A total of 352 infrapopliteal bypasses were performed in 310 patients with CLTI. Fourteen percent of the revascularizations were performed on dialysis patients (48/352). Median age was 73 years (interquartile range - IQR 15) and 74% (259/352) were male. Median follow-up was 26 months (IQR 42). Overall, 92% (325/352) had tissue loss and 44% (154/352) had some degree of infection. The majority of revascularization procedures were performed with vein grafts (61%, 214/352). The 30-day mortality was 4% (11/310), with no difference between groups (p = 0.627). Kaplan-Meier analysis showed no difference between groups regarding freedom from CLTI (76% vs. 79%; HR 0.96, CI 0.65-1.44, p=0.857), limb-salvage (70% vs. 82%; HR 1.40, CI 0.71-2.78, p=0.327) and survival (62% vs. 64%; HR 1.08, CI 0.60-1.94, p=0.799). PP rates were 39% in group 1 and 64% in group 2 (HR 1.71, CI 1.05-2.79, p=0.030). TP rates were not different between groups (57% and 78%; HR 1.79, CI 0.92-3.47, p=0.082). CONCLUSION: Infrapopliteal bypass for CLTI, on dialysis patients, resulted in lower PP rates. No differences were observed in freedom from CLTI, TP, limb salvage and survival.

Integrated Proteomics Characterization of NLRP3 Inflammasome Inhibitor MCC950 in Monocytic Cell Line Confirms Direct MCC950 Engagement with Endogenous NLRP3.

Inhibition of the NLRP3 inflammasome is a promising strategy for the development of new treatments for inflammatory diseases. MCC950 is a potent and selective small-molecule inhibitor of the NLRP3 pathway and has been validated in numerous species and disease models. Although the capacity of MCC950 to block NLRP3 signaling is well-established, it is still critical to identify the mechanism of action and molecular targets of MCC950 to inform and derisk drug development. Quantitative proteomics performed in disease-relevant systems provides a powerful method to study both direct and indirect pharmacological responses to small molecules to elucidate the mechanism of action and confirm target engagement. A comprehensive target deconvolution campaign requires the use of complementary chemical biology techniques. Here we applied two orthogonal chemical biology techniques: compressed Cellular Thermal Shift Assay (CETSA) and photoaffinity labeling chemoproteomics, performed under biologically relevant conditions with LPS-primed THP-1 cells, thereby deconvoluting, for the first time, the molecular targets of MCC950 using chemical biology techniques. In-cell chemoproteomics with inlysate CETSA confirmed the suspected mechanism as the disruption of inflammasome formation via NLRP3. Further cCETSA (c indicates compressed) in live cells mapped the stabilization of NLRP3 inflammasome pathway proteins, highlighting modulation of the targeted pathway. This is the first evidence of direct MCC950 engagement with endogenous NLRP3 in a human macrophage cellular system using discovery proteomics chemical biology techniques, providing critical information for inflammasome studies.

Impact of Extraction Methods and Transportation Conditions on Lipid Profiles of Bovine Oocytes.

Lipids play numerous pivotal physiological roles in mammalian reproduction, being indispensable for oocyte competence acquisition and post-fertilization embryonic development. Profiling lipids in minute samples, such as oocytes, presents challenges but has been accomplished through mass spectrometry technologies like Multiple Reaction Monitoring (MRM) profiling. With the dual objectives of simplifying workflow and examining the influence of preanalytical conditions, we assessed whether transportation at room temperature affects the lipid profile of bovine oocytes. To this end, samples were prepared using either monophasic (methanol only) or biphasic liquid extraction protocols (Bligh & Dyer method) and transported either on dry ice or at room temperature inside sealed-vacuum packages to prevent lipid oxidation. Subsequently, employing a comprehensive method, we screened a list of 316 MRMs from 10 different lipid subclasses in oocyte lipid extracts. Principal Component Analysis (PCA) revealed similar lipid profiles concerning temperature during transportation, whereas clear differentiation among samples was observed based on the lipid extraction method. Univariate analysis indicated that the one-phase methanol extraction resulted in higher relative abundances of phospholipids, except for phosphatidylserines. Conversely, the Bligh & Dyer extraction favored the detection of neutral intracellular lipids (triacylglycerols, free fatty acids, cholesteryl esters, and acyl-carnitines). Consequently, lipid recovery was directly correlated with the polarity of lipid class and the extraction method. Regarding transportation temperature, phosphatidylethanolamine, triacylglycerol, and free fatty acids exhibited lower abundances when samples were transported at room temperature. Based on multivariate and univariate analyses, we conclude that if samples undergo the same lipid extraction protocol and are transported in the same batch at room temperature inside vacuum-sealed bags, it is feasible to analyze lipid extracts of bovine oocytes and still obtain informative lipid profiling results.

Synthesis of superparamagnetic Fe3O4-graphene oxide-based material for the photodegradation of clonazepam.

The global concern over water pollution caused by contaminants of emerging concern has been the subject of several studies due to the complexity of treatment. Here, the synthesis of a graphene oxide-based magnetic material (GO@Fe3O4) produced according to a modified Hummers' method followed by a hydrothermal reaction was proposed; then, its application as a photocatalyst in clonazepam photo-Fenton degradation was investigated. Several characterization analyses were performed to analyze the structure, functionalization and magnetic properties of the composite. A 23 factorial design was used for the optimization procedure to investigate the effect of [H2O2], GO@Fe3O4 dose and pH on clonazepam degradation. Adsorption experiments demonstrated that GO@Fe3O4 could not adsorb clonazepam. Photo-Fenton kinetics showed that total degradation of clonazepam was achieved within 5 min, and the experimental data were better fitted to the PFO model. A comparative study of clonazepam degradation by different processes highlighted that the heterogeneous photo-Fenton process was more efficient than homogeneous processes. The radical scavenging test showed that O 2 · - was the main active free radical in the degradation reaction, followed by hydroxyl radicals (•OH) and holes (h+) in the valence layer; accordingly, a mechanism of degradation was proposed to describe the process.

Effect of lipid extraction and room temperature transportation of bovine oocytes determined by MRM profiling.

Lipids play many important physiological roles in mammalian reproduction, being essential for the acquisition of oocyte competence and post-fertilization embryonic development. Lipid profiling in samples of minute size, such as oocytes, is challenging but has been achieved by mass spectrometry technologies such as multiple reaction monitoring (MRM) profiling. With the goals of further simplifying sample workflow and investigating the influence of pre-analytical conditions, we have evaluated how different extraction methods and transportation of lipid extracts in vacuum and at room temperature impacted the lipid profile of bovine oocytes. Using a comprehensive method, 316 MRMs associated with lipids of 10 different classes were screened in oocyte lipid extracts prepared by 2 extraction methods (one-step methanol addition or Bligh and Dyer) and transporting them in dry ice or at room temperature inside vacuum packages. No changes in the multivariate analysis (PCA) were noticeable due to transportation temperature, while lipid profiles were more affected by the lipid extraction protocol. Sample extraction using pure methanol favored the detection of phospholipids uniformly, while Bligh and Dyer favored the detection of neutral intracellular lipids. Triacylglycerol lipids and free fatty acids yielded decreased abundances when samples were transported at room temperature. We conclude that if samples are submitted to the same lipid extraction protocol and same transportation batch at room temperature coupled with vacuum conditions it is possible to analyze lipid extracts of bovine oocytes and still obtain informative lipid profiling results.

Embarque na residência integrada em saúde: relato de experiências com ênfase em saúde coletiva / Boarding on the integrated health residence: report of experiences with emphasis on public health / Abordaje de la residencia sanitaria integrada: relato de experiencias con énfasis en salud pública

Este trabalho tem o objetivo de relatar a experiência de imersão na especialização com caráter de residência integrada com ênfase em Saúde Coletiva. Trata- se de um estudo descritivo, qualitativo, utilizando-se como referencial teórico para descrição a observação participante, memória afetiva, diário de bordo e da textualização. Foi desenvolvido na Residência Integrada em Saúde, da Escola de Saúde Pública do Estado do Ceará, ênfase em Saúde Coletiva, no período de dois anos, em Fortaleza, Ceará. Os cenários de prática foram setores relacionados à Atenção Primária à Saúde, Vigilância Epidemiológica, Sanitária e Ambiental, Controle de Endemias e Zoonoses, Imunização, além dos espaços de gestão, assistência e controle social. A imersão na especialização em caráter de residência viabilizou ferramentas para a vivência, por meio da interprofissionalidade e integração contínuas, e da realização das atividades, circulação de informações, compreensão das experiências, entendimento de macroprocessos, políticas e indicadores. Rodas de Campo e de Equipe viabilizaram o aprofundamento das temáticas administrativa, formativa, teórica e terapêutica. Percebeu-se resistência e desejo de remodelações, mediante inovação e diálogo intersetorial. A relação entre as categorias profissionais e ser sanitarista foi desafiadora. Oportunizou-se, também, habilidades de gestão, autonomia e governança, com pluralidade formativa e riqueza de atuação. A vivência favoreceu a percepção do programa de residência como processo formativo plural e significativo. O elemento político se destacou como base para a formação dos residentes e fortalecimento do sistema de saúde, e a residência integrada em saúde, como modalidade diferenciada de formação, com desafios e potencialidades.

The object of this study was to report the experience of immersion in the specialization as an integrated residency with emphasis on Public Health. A descriptive, qualitative study, of the experience report type, using participant observation, affective memory, logbook and textualization as theoretical references for description. It was developed in the Integrated Residency in Health, of the School of Public Health. emphasis on Public Health, during two years, in Fortaleza, Ceará, Brazil. The practice scenarios were sectors related to Primary Health Care, Epidemiological, Sanitary and Environmental Surveillance, Endemic and Zoonosis Control, Immunization, in addition to management, assistance and social control spaces. The immersion in the specialization in a residency character has enabled tools for the experience, through continuous interprofessionalism and integration, and the realization of activities, circulation of information, understanding of experiences, understanding of macro processes, policies and indicators. Field and team meetings made it possible to deepen the administrative, formative, theoretical, and therapeutic themes. Resistance and a desire for remodeling was perceived, through innovation and intersectoral dialogue. The relationship between the professional categories and being a sanitarian was challenging. It also provided opportunities for management skills, autonomy, and governance, with formative plurality and richness of action. The experience favored the perception of the residency program as a plural and significant formative process. The political element stood out as the basis for training residents and strengthening the health system, and the integrated health residency as a differentiated training modality, with challenges and potentialities.

El objeto de este estudio fue relatar la experiencia de inmersión en la especialización como residencia integrada con énfasis en Salud Pública. Estudio descriptivo, cualitativo, del tipo relato de experiencias, utilizando la observación participante, la memoria afectiva, la bitácora y la textualización como referentes teóricos para la descripción. Se desarrolló en la Residencia Integrada en Salud, de la Escuela de Salud Pública. énfasis en Salud Pública, durante dos años, en Fortaleza, Ceará, Brasil. Los escenarios de la práctica fueron sectores relacionados con la Atención Primaria de Salud, Vigilancia Epidemiológica, Sanitaria y Ambiental, Control de Endemias y Zoonosis, Inmunización, además de espacios de gestión, asistencia y control social. La inmersión en la especialización en carácter de residencia ha posibilitado herramientas para la experiencia, a través de la continua interprofesionalidad e integración, y la realización de actividades, circulación de información, comprensión de experiencias, entendimiento de macro procesos, políticas e indicadores. Reuniones de campo y de equipo posibilitaron la profundización de los temas administrativos, formativos, teóricos y terapéuticos. Se percibieron resistencias y deseos de remodelación, a través de la innovación y el diálogo intersectorial. La relación entre las categorías profesionales y el ser sanitario fue desafiante. También proporcionó oportunidades para la capacidad de gestión, autonomía y gobernanza, con pluralidad formativa y riqueza de acción. La experiencia favoreció la percepción del programa de residencia como un proceso formativo plural y significativo. Se destacó el elemento político como base para la formación de residentes y fortalecimiento del sistema de salud, y la residencia integrada en salud como modalidad formativa diferenciada, con desafíos y potencialidades.

Comparing follow-up of patients with vitamin K antagonists in a health center: pre- and post-COVID-19 pandemic / Comparação do seguimento de doentes hipocoagulados com antagonistas da vitamina K numa unidade de saúde: pré e pós pandemia COVID-19

Introduction and objectives: During the COVID-19 pandemic, the follow-up of patients treated with vitamin K antagonists (VKAs) may have been affected. This study aims to compare how these patients were monitored pre- and post-COVID-19 pandemic and understand the impact of non-face-to-face appointments on their follow-up. Methods: We conducted a retrospective cohort study in a Portuguese Health Center. The study included patients treated with VKAs and followed at the Health Center for international normalized ratio (INR) monitoring between March 2019 and March 2021. Data collected: sex, age, type of VKA; INR; date of INR assessment, type of appointment (face-to-face or phone/e-mail). Rosendaal's method was used to calculate pre-COVID-19 and post-COVID-19 time in therapeutic range (TTR). Good TTR control was defined if values ≥ 70%. Results: 44 patients were included. The mean TTR in the pre-COVID-19 period was 64.55% (95% CI: 58.10 - 71.00%). The post-COVID-19 mean was slightly higher (+ 2.26%), 66.81% (95% CI: 59.66 - 73.97%), but the difference was not statistically significant (p = 0.576). The use of non-face-to-face appointments did not contribute to worsening post-pandemic TTR, show-ing no lower follow-up than during pre-pandemic period in which all contacts were face-to-face [CI (95%) -0.397 - 0.196 for a reference range -0.489 - 0.693]. Conclusions: The TTR value in both periods was similar and lower than the value defined for effective hypocoagulation. The use of non-face-to-face consultation in the post-COVID-19 period does not seem to have influenced the quality of hypocoagulation (AU).

Introdução e objetivos: Durante a pandemia COVID-19 o acompanhamento de doentes medicados com antagonistas da vitamina K (AVKs) pode ter sido afetado. Este estudo pretende comparar a forma como estes doentes foram monitorizados antes e depois da pandemia COVID-19 e compreender o impacto da consulta não presencial no seu seguimento. Métodos: Estudo de coorte retrospetivo num Centro de Saúde em Portugal. O estudo incluiu doentes tratados com AVKs e seguidos no Centro de Saúde para monitorização do International Normalized Ratio(INR) entre março de 2019 e março de 2021. Dados recolhidos: sexo, idade, tipo de AVK; INR; data da avaliação do INR, tipo de consulta (presencial ou por telefone/e-mail). Foi utilizado o método de interpolação linear de Rosendaal para calcular o tempo em intervalo terapêutico (TTR) pré- e pós-COVID-19. Foi definido um bom controle se valores de TTR ≥ 70%. Resultados: Foram incluídos 44 doentes. A média de TTR no período pré-COVID-19 foi de 64,55% (95% IC: 58,10 - 71,00%). A média pós-COVID-19 foi ligeiramente superior (+ 2,26%), 66,81% (95% IC: 59,66 - 73,97%), mas a diferença não foi estatisticamente significativa (p = 0,576). A utilização da consulta não presencial não contribuiu para o agravamento do TTR no período pós-pandemia, não mostrando um seguimento inferior ao do período pré-pandemia em que todos os contatos foram presenciais [IC (95%) -0,397 - 0,196 para um intervalo de referência -0,489 - 0,693]. Conclusões: O valor de TTR em ambos os períodos foi semelhante e inferior ao valor definido para hipocoagulação eficaz. A utilização da consulta não presencial no período pós-COVID-19 não parece ter influenciado a qualidade da hipocoagulação (AU).

Plano de parto no pré-natal: conhecimento dos enfermeiros da atenção primaria à saúde / Pre-natal delivery plan: nurses' knowledge of primary health care / Plan de parto prenatal: conocimientos de las enfermeras sobre atención primaria de la salud

Introdução: É fundamental implementar medidas direcionadas a assegurar a melhoria da cobertura e da qualidade do acompanhamento do pré-natal, da assistência ao parto e puerpério e da assistência à criança. Objetivos: Identificar o conhecimento dos enfermeiros da Atenção Primária à Saúde sobre o plano de parto; realizar ação educativa com os enfermeiros da Atenção Primária à Saúde acerca do plano de parto e identificar seu impacto; informar e destacar junto aos enfermeiros da Atenção Primária à Saúde a importância e a abordagem do plano de parto durante o Pré-Natal. Método: Trata-se de um estudo, descritivo, exploratório de abordagem mista. O Município de Catanduva tem cerca de 121.862 habitantes e 23 unidades de Saúde para atendimento em Atenção Básica, com 46 enfermeiras atuando diretamente no acompanhamento do Pré-Natal e nas ações educativas com as gestantes. Resultados: Nota-se defasagem quanto ao conhecimento e aplicação do instrumento referente ao plano de parto pelos enfermeiros da Atenção Primária à Saúde, evidenciando a importância da criação de treinamentos e protocolos dentro dessa temática. Conclusão: Com o desenvolvimento do estudo, foi possível concluir o quão importante é o Plano de Parto, tanto para a gestante no âmbito de expressar suas vontades, desejos, sanar suas dúvidas e medos, como também é fundamental para o profissional inserido na assistência, já que ele é um documento escrito e de caráter legal, o que o torna meio de respaldo para possíveis contratempos.(AU)

Introduction: It is essential to implement measures aimed at ensuring improved coverage and quality of prenatal care, childbirth and puerperium care and child care. Objectives: To identify the knowledge of Primary Health Care nurses about the birth plan; to carry out educational action with Primary Health Care nurses about the birth plan and to identify its impact; To inform and highlight the importance and approach of the birth plan during the Prenatal Care with the nurses of the Primary Health Care. Method: This is a descriptive, exploratory study with a mixed approach. The city of Catanduva has about 121,862 inhabitants and 23 health units for primary care, with 46 nurses working directly in the monitoring of prenatal care and educational actions with pregnant women. Results: There is a lag in knowledge and application of the instrument related to the birth plan by nurses in Primary Health Care, showing the importance of creating training and protocols within this theme. Conclusion: With the development of the study, it was possible to conclude how important is the Birth Plan, both for the pregnant woman in the context of expressing her wishes, desires, remedy her doubts and fears, as well as is fundamental for the professional inserted in the assistance, since it is a written and legal document, which makes it a means of support for possible setbacks.(AU)

Introducción: Es fundamental implementar medidas encaminadas a garantizar una mejor cobertura y calidad de la atención prenatal, atención al parto y puerperio y atención al niño. Objetivos: Identificar el conocimiento de los enfermeros de la Atención Primaria de Salud (APS) sobre el plan de parto; realizar actividades educativas con enfermeras de la Atención Primaria de Salud sobre el plan de parto e identificar su impacto; informar y resaltar con las enfermeras de la Atención Primaria de Salud la importancia y el abordaje del plan de parto durante la atención prenatal. Metodo: Se trata de un estudio descriptivo, exploratorio, con enfoque mixto. El Municipio de Catanduva tiene cerca de 121.862 habitantes y 23 Unidades de Salud de Atención Básica, con 46 enfermeras actuando directamente en el prenatal y en actividades educativas con gestantes. Resultados: Existe discrepancia en cuanto al conocimiento y aplicación del instrumento referente al plan de parto por parte de los enfermeros de la Atención Primaria de Salud, destacando la importancia de la creación de capacitaciones y protocolos en esta temática. Conclusión: Con el desarrollo del estudio se pudo concluir cuán importante es el Plan de Parto, tanto para la gestante en el contexto de expresar sus deseos, deseos, resolver sus dudas y temores, como también es fundamental para el profesional insertado en la asistencia, ya que es un documento escrito de carácter legal, lo que lo convierte en un medio de apoyo para posibles contratiempos.(AU)

Application of ozonized oils in human body and oral cavity systems / Aplicação dos óleos ozonizados nos sistemas do corpo humano e cavidade oral

ABSTRACT Wound healing consists of the coordinated cascade of molecular, cellular and biochemical events that interact for tissue recomposition to occur. The interruption or failure in this process can trigger the delay in closing the lesion and chronic wounds, in addition to infections. Ozonotherapy proves to be a promising alternative, capable of assisting in the tissue healing process, modulating the inflammatory response and preventing the development of infection. To present, analyze and discuss, through a literature review, the use of Ozonated Oils in the repair of the human body and oral cavity systems. SciELO, LILACS, MEDLINE and PubMed databases were consulted, using the following DeCS / MeSH descriptors: "ozonized oil"; "healing"; "ozone therapy". After crossing, counting and analyzing, 13 studies were selected, according to the theme. Due to the complementary theoretical foundation, 44 articles were included, totaling 57 articles used for the present review. Ozonized Oil therapy has beneficial clinical effects, which include antimicrobial, immunostimulating, analgesic, antipoxic, immunoregulatory and antioxidant action. Topical management of ozone has proven effective for the treatment of foot-and-mouth disease, gingivitis, ulcers and dermatitis, contributing to pain relief and healing. Infectious skin diseases, abscesses, allergic skin diseases, scaly erythema, psoriasis and palmoplantar pustulosis are pathological conditions, the treatment of which benefits from the application of Ozonized Oils. Ozonized Oils have been shown to be effective in the treatment of local infections, when in appropriate formulations and in controlled cases, being a promising therapeutic alternative for tissue repair.

RESUMO A cicatrização de feridas consiste na coordenada cascata de eventos moleculares, celulares e bioquímicos que interagem para que ocorra a recomposição tecidual. A interrupção ou falha nesse processo, pode desencadear o atraso no fechamento da lesão e feridas crônicas, além de infecções. A Ozonioterapia revela-se uma alternativa promissora, capaz de auxiliar no processo de cicatrização tecidual, modular a resposta inflamatória e prevenir o desenvolvimento de infecção. Apresentar, analisar e discutir, através de uma revisão da literatura, o emprego de Óleos Ozonizados no reparo dos sistemas do corpo humano e cavidade oral. Bases de dados SciELO, LILACS, MedLine e PubMed foram consultadas, utilizando os seguintes descritores DeCS/MeSH: "ozonized oil"; "healing"; "ozone therapy". Após cruzamento, apuração e análise, foram selecionados 13 estudos, de acordo com a temática. Devido à fundamentação teórica complementar, 44 artigos foram incluídos, totalizando 57 artigos utilizados para a presente revisão. A terapia com Óleo Ozonizado possui efeitos clínicos benéficos, que incluem ação antimicrobiana, imunoestimulante, analgésica, antipóxica, imunorreguladora e antioxidante. O manejo tópico de ozônio revelou-se eficaz para o tratamento de estomatite aftosa, gengivite, úlceras e dermatite, contribuindo para o alívio da dor e cicatrização. Doenças infecciosas da pele, abscessos, doenças alérgicas da pele, eritema escamoso, psoríase e pustulose palmoplantar são condições patológicas, cujo tratamento é beneficiado pela aplicação de Óleos Ozonizados. Os Óleos Ozonizados têm demonstrado eficácia no tratamento de infecções locais, quando em formulações apropriadas e em casos controlados, sendo uma alternativa terapêutica promissora para o reparo de tecidos.

Effects of Photodynamic Therapy with Laser Photobiomodulation as an Adjunct to Scaling and Root Planing: Systematic Review / Efeitos da Terapia Fotodinâmica com Fotobiomodulação Laser Adjuvante a Raspagem e Alisamento Radicular: Revisão Sistemática

Scaling and root planing is the gold standard procedure in the periodontal disease treatment. Although this therapy has the ability to reduce or eliminate periodontopathogens mthrough periodontal instrumentation, sometimes the response to treatment may not promote the complete eradication of pathogens, affecting the healing process and leading to the presence of residual periodontal pockets. The aim of this study was to analyze the effectiveness of photodinamic therapy with laser photobiomodulation and as an adjunct to scaling and root planing (having as a conductive agent a fiber optic tip radiating inside the periodontal pocket), compared to scaling and root planing alone in patients with periodontitis on randomized trials. This systematic review was carried out complying with the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta Analyses. The databases used were Pubmed, Lilacs, Biblioteca Virtual em Saúde - Odontologia and Medline. "Periodontal Disease" OR "Periodontal Treatment" AND m"Photodinamic Theraphy" were the descriptors used. A total of five articles were included. Two studies have shown superior results in relation to the referred association; a study indicated benefits in biochemical and/or microbiological aspects; it suggests, however, that clinical relevance needs to be better evaluated; two studies did not reveal benefits that categorize photodynamic therapy + scaling and root planning as superior to conventional treatment. The association in question, when well standardized and performed, can result in promising responses in the control of periodontal disease; however, the clinical benefits that demonstrate its superiority will certainly be subject to new researches. (AU)

A raspagem e alisamento radicular é o procedimento padrão-ouro no tratamento da doença periodontal. Embora essa terapia seja capaz de reduzir ou eliminar os periodontopatógenos por meio da instrumentação periodontal, algumas vezes a resposta ao tratamento pode não promover completa erradicação dos patógenos, afetando assim, o processo de cicatrização e levando à presença de bolsas periodontais residuais. O objetivo do presente estudo foi analisar a eficácia da terapia fotodinâmica com fotobiomodulação laser adjuvante a raspagem e alisamento radicular (tendo como agente condutor uma ponta de fibra óptica que irradia o interior da bolsa periodontal), em comparação com a raspagem e alisamento radicular isoladamente em pacientes com periodontite, avaliados em estudos clínicos randomizados. Esta revisão sistemática foi realizada de acordo com as recomendações Principais Itens para Relatar Revisões sistemáticas e Meta-análises. As bases de dados utilizadas foram Pubmed, Lilacs, Biblioteca Virtual em Saúde - Odontologia e Medline. "Doença periodontal" OR "Tratamento periodontal" AND "Terapia fotodinâmica" foram os descritores utilizados. Um total de cinco artigos foram incluídos. Dois estudos mostraram resultados superiores em relação à referida associação; um estudo indicou benefícios nos aspectos bioquímicos e/ou microbiológicos; sugere, no entanto, que a relevância clínica precisa ser melhor avaliada; dois estudos não revelaram benefícios que categorizassem a terapia fotodinâmica + raspagem e alisamento radicular como superiores ao tratamento convencional. A associação em questão, quando bem padronizada e realizada, pode resultar em respostas promissoras no controle da doença periodontal; entretanto, os benefícios clínicos que demonstram sua superioridade certamente serão alvo de novas pesquisas. (AU)

Different tray cell volumes for lettuce grown in conventional and hydroponic system / Diferentes volumes de célula da bandeja para alface cultivada em sistema convencional e hidropônico

ABSTRACT: Nowadays, an important economic movement is involved in the improvement of polyethylene trays for the production of seedlings, being developed with different volumes, numbers and cell formats in an each culture-specific manner. The objective of the present study was to evaluate the performance of curly lettuce originated from seedlings from trays with different volumes, through the comparison between conventional and hydroponic-NFT cultivation. Five volumes of trays (10, 20, 30, 40 and 50 cm³ of cell-1) were tested in conventional and hydroponic cultivation, from February to March 2016. For the purpose of comparison, the plants were evaluated through the characteristics of height, circumference, stem diameter, number of leaves, fresh shoot mass and productivity. The use of volumes greater than 10 cm³ showed higher precocity in both cultivation systems, with hydroponic cultivation being more precocious and productive when compared to conventional cultivation.

RESUMO: Atualmente, um importante movimento econômico está envolvido na aprimoração das bandejas de polietileno para a produção de mudas, sendo desenvolvido com diferentes volumes, números e formatos de células de forma específica para cada cultura. O objetivo do presente estudo foi avaliar o desempenho de alface crespa, oriunda de mudas de bandejas com diferentes volumes, através da comparação entre cultivo convencional e hidropônico-NFT. Cinco volumes de bandejas (10, 20, 30, 40 e 50 cm³ de célula-1) foram testados em cultivo convencional e hidropônico, no período de fevereiro a março de 2016. Para fins de comparação, as plantas foram avaliadas através das características de altura, circunferência, diâmetro do caule, número de folhas, massa fresca da parte aérea e produtividade. O uso de volumes superiores a 10 cm³ apresentou maior precocidade em ambos os sistemas de cultivo, sendo o cultivo hidropônico mais precoce e produtivo quando comparado ao cultivo convencional.

Influência da idade do lodo na colmatação das membranas em um biorreator à membrana tratando esgoto sanitário / Effect of solids retention time on membrane fouling in a membrane bioreactor treating municipal wastewater

RESUMO O presente estudo avaliou o efeito da idade do lodo (θc) no potencial incrustante do licor misto em um biorreator à membrana (BRM) tratando esgoto sanitário. Tal avaliação foi conduzida em BRM construído em escala de bancada, com volume útil de 15 L, operado por 420 dias na modalidade de batelada sequencial. Durante o período experimental, foram aplicadas 3 estratégias operacionais, E-1, E-2 e E-3, em que foram testadas as idades de lodo de 80, 40 e 20 dias, respectivamente. Os resultados revelaram que a utilização da idade de lodo de 20 dias resultou em licor misto com maior potencial incrustante, apresentando, neste caso, uma velocidade de colmatação (VC) das membranas de 1,95 mbar dia-1, aproximadamente 2 vezes maior do que a observada nas idades de lodo de 80 e 40 dias. A maior colmatação observada foi atribuída a maior concentração de produtos microbianos solúveis (PMSs) no licor misto e a maior relação proteínas/polissacarídeos (PN/PS) dos flocos biológicos nesse período em questão. Por outro lado, a aplicação da idade de lodo de 80 dias resultou em menor VC das membranas do BRM, com valor de 0,82 mbar dia-1. Contudo, no período final dessa estratégia foi observado crescimento excessivo de bactérias filamentosas, que se refletiu em piora da filtrabilidade do licor misto e aumento da VC das membranas. De maneira geral, os resultados obtidos mostraram que a aplicação da idade de lodo de 40 dias resultou em licor misto com menor potencial incrustante.

ABSTRACT This study evaluated the effect of solids retention time (SRT) on membrane fouling rate in a membrane bioreactor (MBR) treating municipal wastewater. The evaluation was conducted in a membrane bioreactor built in bench scale, with a volume of 15 L, operated for 420 days in the sequential batch regime. During this period, three experimental runs were applied, E-1, E-2 and E-3, in which the solids retention time of 80, 40 and 20 days, respectively, were tested. The results showed that use of 20-days solids retention time resulted in a higher membrane fouling rate (MFR), with value of 1,95 mbar d-1, approximately two times higher than observed in the solids retention time of 80 and 40 days. The higher membrane fouling rate observed was attributed to a higher concentration of soluble microbial products (SMP) in the mixed liquor and to the higher proteins/polysaccharides ratio of the biological flocs in this period. On the other hand, the use of 80-days solids retention time resulted in a lower membrane fouling rate, with a value of 0.82 mbar d-1. However, it was observed in the final period of this experimental run an excessive growth of filamentous bacteria, which was reflected in a deterioration of the mixed liquor filterability and an increase of membrane fouling rate. Overall, the results showed that the 40-days solids retention time resulted in a mixed liquor with lower fouling propensity.

Lesões de pele em recém-nascido: revisão integrativa / Skin lesions in newborns: integrative review

O objetivo é descrever e analisar as evidências cientificas acerca de lesões de pele em recém-nascido. Buscouse produções científicas tendo como critérios de inclusão publicações período de 2012 a 2017, que correlacionassem os descritores "pele", "ferimentos e lesões" e "recém-nascido". As bases de dados pesquisadas foram PUBMED, WEB OF SCIENCE, LILACS, CINAHL e SCIELO. Ao analisarem-se os 10 estudos selecionados, foi consenso entre os autores que a produção de conhecimento científico referente à temática de lesões de pele em RN representa um eixo norteador para a assistência. A integridade da pele é de grande importância para a sobrevida do RN. Em virtude da sua atuação entre os órgãos internos e o meio externo, a pele atua como barreira contra infecção. Os fatores de risco para lesões de pele em RN estão associados aos procedimentos realizados no atendimento hospitalar. Conclui se que na literatura pesquisada, estudos que abordem os cuidados com a pele do RN diante de lesões ainda são escassos, portanto, faz-se necessário desenvolver novos estudos nesse sentido

The objective is to describe and analyze the scientific evidence about skin lesions in newborns. It was searched for scientific productions having as criteria of inclusion publications period from 2012 to 2017, that correlated the descriptors "skin", "injuries and lesions" and "newborn". The searched databases were PUBMED, WEB OF SCIENCE, LILACS, CINAHL and SCIELO. When analyzing the 10 selected studies, it was agreed among the authors that the production of scientific knowledge regarding the theme of skin lesions in NB represents a guiding axis for the care. The integrity of the skin is of great importance for the survival of the newborn. Due to its action between the internal organs and the external environment, the skin acts as a barrier against infection. Risk factors for skin lesions in newborns are associated with procedures performed in hospital care. It is concluded that in the researched literature, studies that address the skin care of the newborn with lesions are still scarce, therefore, it is necessary to develop new studies in this sense

Arboviroses reemergentes: perfil clínico-epidemiológico de idosos hospitalizados / Arbovirosis reemergentes: perfil clínico epidemiológico de personas mayores hospitalizadas / Reemerging arboviruses: clinical-epidemiological profile of hospitalized elderly patients

RESUMO Objetivo: Descrever o perfil clínico-epidemiológico de idosos hospitalizados com arboviroses. Método: Estudo documental, retrospectivo, de base populacional e descritivo, com abordagem quantitativa, realizado com idosos hospitalizados com diagnóstico de arbovirose em um hospital universitário. Os dados foram coletados por meio de consulta aos prontuários e fichas de notificação. Resultados: Participaram 33 idosos. Houve prevalência da Dengue, destacando-se a febre, a mialgia e a artralgia. Verificaram-se a Hipertensão Arterial e a Diabetes como comorbidades. Foram evidenciadas associações estatisticamente significativas da arbovirose com a escolaridade, a situação profissional, o estado civil, os resultados de exames e o uso de analgésicos; e entre o local da artralgia e a Chikungunya. Conclusão: Os resultados fornecem subsídios para a assistência do enfermeiro aos idosos hospitalizados com arboviroses, permitindo a elaboração de um plano de cuidados adequado e humanizado.

RESUMEN Objetivo: Describir el perfil clínico epidemiológico de personas mayores hospitalizadas con arbovirosis. Método: Estudio documental, retrospectivo, de base poblacional y descriptivo, con abordaje cuantitativo, realizado con personas mayores hospitalizadas con diagnóstico de arbovirosis en un hospital universitario. Los datos fueron recogidos mediante consulta a las fichas médicas y de notificación. Resultados: Participaron 33 personas mayores. Hubo prevalencia del Dengue, destacándose la fiebre, la mialgia y la artralgia. Se verificaron la Hipertensión Arterial y la Diabetes como comorbilidades. Fueron evidenciadas asociaciones estadísticamente significativas de la arbovirosis con la escolaridad, la situación profesional, el estado civil, los resultados de exámenes y el empleo de analgésicos; y entre el sitio de la artralgia y la Chikungunya. Conclusión: Los resultados brindan subsidios para la asistencia del enfermero a las personas mayores hospitalizadas con arbovirosis, permitiendo la elaboración de un plan de cuidados adecuado y humanizado.

ABSTRACT Objective: Describe the clinical-epidemiological profile of hospitalized elderly patients with arbovirus. Method: A documentary retrospective population-based descriptive study that used a quantitative approach with hospitalized elderly patients diagnosed with arbovirus was conducted in a teaching hospital. Data were collected from medical records and investigation forms. Results: Thirty-three elderly patients participated in this study. A prevalence of dengue was observed, with fever, myalgia, and arthralgia. Arterial hypertension and diabetes were the comorbidities. Statistically significant correlations were obtained between arbovirus and schooling, employment situation, marital status, test results, and use of analgesics; and between the site of arthralgia and Chikungunya. Conclusion: The results support nursing care to hospitalized elderly patients with arbovirus, allowing the development of a proper and humanized care plan.

Qualidade de vida de pacientes com insuficiência renal crônica submetidos à hemodiálise / Quality of life of patients with chronic renal failure undergoing hemodialysis

Este artigo objetivaavaliar a qualidade de vida de pacientes com insuficiência renal crônica em tratamento de hemodiáliseTrata-se de umestudo transversal e descritivo, com abordagem quantitativa, realizado com 40 pessoas em tratamento hemodialítico em um centro de referência. A coleta de dados ocorreu mediante entrevista, utilizando instrumento semiestruturado e WHOQOLbref. Não foram identificadas associações estatisticamente significativas entre a qualidade de vida e as variáveis sociodemográficas. O domínio Relações sociais apresentou a maior média (63,7) e o domínio Físico sofreu maior prejuízo na qualidade de vida, exibindo média de 45,4. Foi evidenciada uma redução na qualidade de vida dos pacientes renais crônicos submetidos a terapia renal substitutiva, sendo necessário que o profissional de saúde esteja preparado para cuidar dos problemas e dificuldades enfrentadas por essas pessoas. No âmbito da assistência em nefrologia, esta pesquisa propicia uma reflexão acerca de intervenções que visem melhorar a qualidade de vida dos pacientes submetidos ao tratamento hemodialítico

This article aims to evaluate the quality of life of patients with chronic renal failure in hemodialysis treatment. This is a cross-sectional and descriptive study, with a quantitative approach, performed with 40 people on hemodialysis treatment in a reference center. Data were collected through an interview using a semi-structured instrument and WHOQOL-bref. No statistically significant associations were found between quality of life and sociodemographic variables. The Social relations domain presented the highest mean (63.7) and the Physical domain suffered the greatest loss of quality of life, with an average of 45.4. It was evidenced a reduction in the quality of life of chronic renal patients submitted to renal replacement therapy, being necessary that the health professional be prepared to take care of the problems and difficulties faced by these people. In the context of care in nephrology, this research provides a reflection on interventions aimed at improving the quality of life of patients undergoing hemodialysis treatment

Aprendizagem significativa na educação em enfermagem: uma revisão integrativa / Meaningful learning in nursing education: an integrative review / Aprendizaje significativo en la formación de enfermería: una revisión integrativa

Objetivo: Analisar a produção científica acerca das estratégias educativas, baseadas em evidências, utilizadas no processo de ensino aprendizagem em enfermagem à luz da abordagem da aprendizagem significativa. Método: Revisão integrativa desenvolvida de acordo com as etapas: identificação do tema e seleção da hipótese de pesquisa; estabelecimento dos critérios de elegibilidade; identificação dos estudos nas bases científicas/categorização dos estudos; avaliação dos estudos selecionados; análise crítica e interpretação dos resultados; apresentação dos dados na estrutura da revisão integrativa. Resultados: Diferentes estratégias educativas baseadas na aprendizagem significativa foram identificadas tais como: mapa conceitual, estudo de caso, uso de simulador, cursos on-line e jogos. Como principais resultados das intervenções, observou-se a promoção da aprendizagem significativa e o desenvolvimento do raciocínio reflexivo e crítico em estudantes e profissionais de enfermagem, atuando como sujeitos de sua própria práxis. Conclusão: A aprendizagem significativa é uma linha pedagógica fundamental, devendo ser inserida no processo de educação em diversas áreas do saber. Dessa maneira, foi possível identificar que a mesma ancorou estratégias de ensino que se mostraram eficazes, com destaque para o uso de mapas conceituais e as tecnologias educacionais digitais.

Objective: To analyze the scientific production about educational strategies, based on evidence, used in the teaching-learning process in nursing in the light of the meaningful learning approach. Method: Integrative review developed according to the steps: identification of the theme and selection of the research hypothesis; establishment of eligibility criteria; identification of studies in scientific bases/categorization of studies; evaluation of selected studies; critical analysis and interpretation of results; presentation of data in the framework of the integrative review. Results: Different educational strategies based on meaningful learning were identified, such as: conceptual map, case study, use of simulator, online courses and games. As main results of the interventions, it was observed the promotion of meaningful learning and the development of reflective and critical reasoning in students and nursing professionals, acting as subjects of their own praxis. Conclusion: Meaningful learning is a fundamental pedagogical line and should be inserted in the education process in several areas of knowledge. In this way, it was possible to identify that it anchored teaching strategies that proved to be effective, with emphasis on the use of concept maps and digital educational technologies.

Objetivo: Analizar la producción científica sobre estrategias educativas basadas en la evidencia utilizadas en el proceso de enseñanza-aprendizaje en enfermería a la luz del enfoque del aprendizaje significativo. Método: Revisión integrativa desarrollada de acuerdo con los siguientes pasos: identificación del tema y selección de la hipótesis de investigación; establecimiento de criterios de elegibilidad; identificación de estudios en bases de datos científicas/categorización de estudios; evaluación de los estudios seleccionados; análisis crítico e interpretación de los resultados; presentación de los datos en la estructura de revisión integrativa. Resultados: Se identificaron distintas estrategias educativas basadas en el aprendizaje significativo como: mapa conceptual, estudio de casos, uso de simulador, cursos online y juegos. Los principales resultados de las intervenciones fueron la promoción del aprendizaje significativo y el desarrollo del pensamiento reflexivo y crítico en estudiantes y profesionales de enfermería, actuando como sujetos de su propia praxis. Conclusión: El aprendizaje significativo es una línea pedagógica fundamental y debe insertarse en el proceso educativo en diversas áreas del conocimiento. Así, fue posible identificar que ancló estrategias de enseñanza que se mostraron eficaces, con énfasis en el uso de mapas conceptuales y tecnologías educativas digitales.