Irisin attenuates ventilator-induced diaphragmatic dysfunction by inhibiting endoplasmic reticulum stress through activation of AMPK.

Mechanical ventilation (MV) is an essential life-saving technique, but prolonged MV can cause significant diaphragmatic dysfunction due to atrophy and decreased contractility of the diaphragm fibres, called ventilator-induced diaphragmatic dysfunction (VIDD). It is not clear about the mechanism of occurrence and prevention measures of VIDD. Irisin is a newly discovered muscle factor that regulates energy metabolism. Studies have shown that irisin can exhibit protective effects by downregulating endoplasmic reticulum (ER) stress in a variety of diseases; whether irisin plays a protective role in VIDD has not been reported. Sprague-Dawley rats were mechanically ventilated to construct a VIDD model, and intervention was performed by intravenous administration of irisin. Diaphragm contractility, degree of atrophy, cross-sectional areas (CSAs), ER stress markers, AMPK protein expression, oxidative stress indicators and apoptotic cell levels were measured at the end of the experiment.Our findings showed that as the duration of ventilation increased, the more severe the VIDD was, the degree of ER stress increased, and the expression of irisin decreased.ER stress may be one of the causes of VIDD. Intervention with irisin ameliorated VIDD by reducing the degree of ER stress, attenuating oxidative stress, and decreasing the apoptotic index. MV decreases the expression of phosphorylated AMPK in the diaphragm, whereas the use of irisin increases the expression of phosphorylated AMPK. Irisin may exert its protective effect by activating the phosphorylated AMPK pathway.

Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging.

We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a long-working-distance miniature objective to enable multi-plane imaging over a volume of 420 × 420 × 180 µm3 at a lateral resolution of ~1 µm. Together with the detachable design that permits long-term recurring imaging, our miniature two-photon microscope can help decipher neuronal mechanisms in freely behaving animals.

Stability-enhanced low-frequency fiber optic hydrophone based on an extrinsic Fabry-Perot interferometer.

Fiber optic hydrophones (FOHs) offer the notable advantage of electromagnetic interference resistance. Nevertheless, overcoming the challenge of sustaining stable, high-performance operation in intricate underwater settings at a low cost remains a considerable obstacle for them. To circumvent the restrictions noted above, we employed a miniaturized FOH, utilizing an easily fabricated extrinsic Fabry-Perot interferometer (EFPI) which is made up of a composite chromium-aluminum (Cr-Al) membrane and fiber. The linear demodulation also suppresses the drift issue in the output spectrum. The average sound pressure sensitivity of the sensor, according to experimental findings, is around -139.15 dB re 1 V/µPa, while the equivalent noise sound pressure at 1 kHz is 51.52 dB re 1 µPa/Hz1/2. This sensor has a lot of potential because of features like sensitive low-frequency response and noise performance.

Aggregation-Induced Near-Infrared Emission and Electrochemiluminescence of an Iridium(III) Complex for Ampicillin Sodium Sensing.

A new iridium(III) complex was synthesized and characterized. Its photophysical properties and aggregation-induced emission and electrochemiluminescence in the near-infrared range were studied. The large conjugated cyclometallic ligand 1,2-phenylbenzoquinoline (pbq) was selected to form the Ir-C bond with the metal iridium(III) center and provide near-infrared emission of the complex. The auxiliary ligand 4,4'-diamino-2,2'-bipyridine (dabpy) can form hydrogen bonds, which was beneficial for the generation of aggregation-induced emission. The complex was aggregated into small spherical nanoparticles in 80% water and fascinating nanorings in 90% water. The sensing of ampicillin sodium (AMP) antibiotic by the iridium(III) complex were also investigated by photoluminescent and electrochemiluminescent methods. The complex showed a good selectivity toward AMP antibiotic compared to sodium phenylacetate and other eight antibiotics. The detection limits for AMP antibiotic was 0.76 µg/mL. This work provided a new strategy for the design of iridium(III) complex-based aggregation-induced emission and electrochemiluminescence probes for the sensing application.

Fabrication and characterization of gelatin-EGCG-pectin ternary complex: formation mechanism, emulsion stability, and structure.

BACKGROUND: Protein-polyphenol-polysaccharide ternary complex particles have better emulsion interfacial stability compared to protein-polysaccharide binary complexes. However, knowledge is scarce when it comes to the fabrication of protein-polyphenol-polysaccharide ternary complexes as interfacial stabilizers and the interactions between the three substances. In the present work, ternary complexes were prepared using gelatin, high methoxyl pectin, and epigallocatechin gallate (EGCG) as raw materials. The effect of different influencing factors on the formation process of ternary complexes was investigated by varying different parameters. physicochemical stability, emulsifying properties, and structural characteristics were analyzed. RESULTS: The ternary complex had a smaller particle size (275 nm) and polydispersity index (0.112) when the mass concentration ratio of gelatin to high methoxyl pectin was 9:1, addition of EGCG was 0.05%, pH value was 3.0, and ionic strength was 10 mmol L-1 . Meanwhile, the complex had the highest emulsifying stability index (691.75 min) and emulsifying activity index (22.96 m2 g-1 ). Scanning electron microscopical observation demonstrated that the addition of EGCG promoted the dispersion of ternary complex more uniformly, and effectively reduced the agglomeration phenomenon. The discrepancy in fluorescence intensity suggested that interactions between EGCG and gelatin occurred, which altered the protein spatial conformation of gelatin. Fourier transform infrared spectroscopic analysis elucidated that hydrogen bond interaction was the primary non-covalent interaction between EGCG and gelatin-high methoxyl pectin binary complex. CONCLUSION: The aforementioned results purposed to provide some theoretical reference and basis for the rational design of stable protein-polyphenol-polysaccharide ternary complexes. © 2022 Society of Chemical Industry.

A Robust Approach to In Situ Exsolve Highly Dispersed and Stable Electrocatalysts.

Catalysts made of in situ exsolved metal nanoparticles often demonstrate promising activity and high stability in many applications. However, the traditional approach is limited by perovskites as prevailing precursor and requires high temperature typically above 900 K. Here, with the guidance of theoretical calculation, an unprecedented and substantially facile technique is demonstrated for Cu nanoparticles exsolved from interstitially Cu cations doped nickel-based hydroxide, which is accomplished swiftly at room temperature and results in metal nanoparticles with a quasi-uniform size of 4 nm, delivering an exceptional CO faradaic efficiency of 95.6% for the electrochemical reduction of CO2 with a notable durability. This design principle is further proven to be generally applicable to other metals and foregrounded for guiding the development of advanced catalytic materials.

Spontaneous Formation of Ordered Magnetic Domains by Patterning Stress.

The formation of ordered magnetic domains in thin films is important for the magnetic microdevices in spin-electronics, magneto-optics, and magnetic microelectromechanical systems. Although inducing anisotropic stress in magnetostrictive materials can achieve the domain assembly, controlling magnetic anisotropy over microscale areas is challenging. In this work, we realized the microscopic patterning of magnetic domains by engineering stress distribution. Deposition of ferromagnetic thin films on nanotrenched polymeric layers induced tensile stress at the interfaces, giving rise to the directional magnetoelastic coupling to form ordered domains spontaneously. By changing the periodicity and shape of nanotrenches, we spatially tuned the geometric configuration of domains by design. Theoretical analysis and micromagnetic characterization confirmed that the local stress distribution by the topographic confinement dominates the forming mechanism of the directed magnetization.

Impact of selenium on cerebellar injury and mRNA expression in offspring of rat exposed to methylmercury.

During the fetal development stage, the Central Nervous System (CNS) is particularly sensitive to methylmercury (MeHg). However, the mechanism underlying the antagonistic effect of selenium (Se) on MeHg toxicity is still not fully understood. In this study, female rat models with MeHg and Se co-exposure were developed. Pathological changes in the cerebellum and differential mRNA expression profiles in offspring rats were studied. In the MeHg-exposed group, a large number of Purkinje cells showed pathological changes and mitochondria were significantly swollen; co-exposure with Se significantly improved the structure and organization of the cerebellum. In total, 378 differentially expressed genes (DEGs) (including 284 up-regulated genes and 94 down-regulated genes) in the cerebellum of the MeHg-exposed group and 210 DEGs (including 84 up-regulated genes and 126 down-regulated genes) in the cerebellum of the MeHg+Se co-exposed group were identified. The genes involved in neurotransmitter synthesis and release and calcium ion balance in the cerebellum were significantly up-regulated in the MeHg-exposed group. These genes in the MeHg+Se co-exposed group were not changed or down-regulated. These findings demonstrate that the neurotoxicity caused by MeHg exposure is related to the up-regulation of multiple genes in the nerve signal transduction and calcium ion signal pathways, which are closely related to impairments in cell apoptosis and learning and memory. Supplementation with Se can mitigate the changes to related genes and protect neurons in the mammalian brain (especially the developing cerebellum) from MeHg toxicity. Se provides a potential intervention strategy for MeHg toxicity.

TNNT1, a prognostic indicator in colon adenocarcinoma, regulates cell behaviors and mediates EMT process.

Slow skeletal muscle troponin T (TNNT1) has been reported to be correlated with several cancers, but there are no evidences proving that TNNT1 is required in colon adenocarcinoma (COAD). TNNT1 expression in COAD tissues and its prognostic significance were acquired from TCGA database. The proliferative, migratory, and invasive abilities of COAD cells were detected by CCK-8 and transwell assays, respectively. Correlations between TNNT1 and epithelial-mesenchymal transition (EMT)-related markers were determined using western blotting and Pearson's analysis. Our results stated that TNNT1 expression was high-regulated in COAD tissues, which was related with unfavorable prognosis of COAD patients. Functional analyses suggested that TNNT1 promoted the cellular behaviors. Moreover, aberrant expression of TNNT1 affected the expression level of EMT-related proteins. And TNNT1 was negatively linked with E-cadherin. In conclusion, our findings indicated that TNNT1 may promote the progression of COAD, mediating EMT process, and thus shed a novel light on COAD therapeutic treatments.

Real-Time Detection of Orbital Maneuvers Using Epoch-Differenced Carrier Phase Observations and Broadcast Ephemeris Data: A Case Study of the BDS Dataset.

The orbital maneuvers of the global navigation satellite system (GNSSs) have a significant influence on the performance of the precise positioning, navigation, and timing (PNT) services. Because the Chinese BeiDou Navigation Satellite System (BDS) has three types of satellites in the geostationary orbit (GEO), inclined geosynchronous orbit (IGSO), and medium earth orbit (MEO) maneuvers occur more frequently. Thus, it is essential to determine an effective approach for the detection of orbital maneuvers. This study proposes a method for the detection of orbital maneuvers using epoch-differenced carrier phase observations and broadcast ephemeris data. When using the epoch-differenced velocity estimation as a basic data solution model, the time discrimination and satellite identification factors are defined and used for the real-time detection of the beginning and the pseudorandom noise code (PRN) of satellites. The datasets from four GNSS stations (WUH1, BJF1, POHN, CUT0) from the year 2016 were collected and analyzed. The validations showed that the beginning, the PRN of the orbital maneuver of the satellite can be precisely detected in real time for all GEO, IGSO, and MEO satellites, and the detected results also showed good consistency, with the beginning time at a difference of 1-2 min across different stations. The proposed approach was observed to be more sensitive, and the detected beginning time was about 30 min earlier than the single point positioning approach when the high-precision carrier phase observation was used. Thus, orbital maneuvering can be accurately detected by the proposed method. It not only improves the utilization of the collected data but also improves the performance of PNT services.

An Improved Long-Period Precise Time-Relative Positioning Method Based on RTS Data.

The high precision positioning can be easily achieved by using real-time kinematic (RTK) and precise point positioning (PPP) or their augmented techniques, such as network RTK (NRTK) and PPP-RTK, even if they also have their own shortfalls. A reference station and datalink are required for RTK or NRTK. Though the PPP technique can provide high accuracy position data, it needs an initialisation time of 10-30 min. The time-relative positioning method estimates the difference between positions at two epochs by means of a single receiver, which can overcome these issues within short period to some degree. The positioning error significantly increases for long-period precise positioning as consequence of the variation of various errors in GNSS (Global Navigation Satellite System) measurements over time. Furthermore, the accuracy of traditional time-relative positioning is very sensitive to the initial positioning error. In order to overcome these issues, an improved time-relative positioning algorithm is proposed in this paper. The improved time-relative positioning method employs PPP model to estimate the parameters of current epoch including position vector, float ionosphere-free (IF) ambiguities, so that these estimated float IF ambiguities are used as a constraint of the base epoch. Thus, the position of the base epoch can be estimated by means of a robust Kalman filter, so that the position of the current epoch with reference to the base epoch can be obtained by differencing the position vectors between the base epoch and the current one. The numerical results obtained during static and dynamic tests show that the proposed positioning algorithm can achieve a positioning accuracy of a few centimetres in one hour. As expected, the positioning accuracy is highly improved by combining GPS, BeiDou and Galileo as a consequence of a higher amount of used satellites and a more uniform geometrical distribution of the satellites themselves. Furthermore, the positioning accuracy achieved by using the positioning algorithm here described is not affected by the initial positioning error, because there is no approximation similar to that of the traditional time-relative positioning. The improved time-relative positioning method can be used to provide long-period high precision positioning by using a single dual-frequency (L1/L2) satellite receiver.

Analyzing the Satellite-Induced Code Bias Variation Characteristics for the BDS-3 Via a 40 m Dish Antenna.

The satellite-induced code bias variation of geostationary satellite orbit satellites and medium earth orbit satellites of the second-generation BeiDou Navigation Satellite System (BDS-2) exceeds 1 m, which severely affects the accuracy and stability of the ambiguity resolution and high-precision positioning. With the development of the third-generation BDS (BDS-3) with a new system design and new technology, analysis of the satellite-induced code variation characteristics of BDS-3 has become increasingly important. At present, many scholars have explored the satellite-induced code bias of BDS-3, but most of them focus on BDS-3 experimental satellites via normal geodetic antenna. Compared to normal geodetic antenna, the 40-m dish antenna from the National Time Service Center can accurately detect satellite-induced code variations with low noise and high gain. Thus, observational data from fifteen BDS-3 medium earth orbit satellites are collected with the B1I/B2b/B3I/B1C/B2a frequency bands on the day of year (DOY) 199-206 in 2019, the PRN numbers of which are C19/C20/C21/C22/C23/C24/C25/C26/C27/C28/C30/C32/C33 /C35/C37, via the 40 m dish antenna to analyze the code bias variation characteristics. The results show that the obvious satellite-induced elevation­dependent code bias variations exist in the B1I/B2b/B3I/B1C/B2a frequency bands of C28, compared with other satellites. Similarly, the multipath (MP) combination of B3I has an obvious elevation­dependent variation within a range of 0.1 m for C21/C24/C27/C28/C37 and elevation­dependent variation of the B2a and B2b frequency bands also exists in most satellites with a range of 0.1 m. However, the MP combination values of some satellites are asymmetric with respect to elevation, which is different from BDS-2 satellites and especially obvious for BDS-3 satellites B1I and BIC frequency bands with elevation­dependent variations of 0.2 m, indicating that the code bias variation is not uniquely related to elevation, especially for the B1I/BIC frequency bands. What's more, the satellite-induced code bias variation of the BDS-3 satellites is greatly reduced compared with that of the BDS-2 satellites. In addition, the similar code bias variation appears at the Xia1 station with a normal geodetic antenna of B1I/B1C/B3I/B2a/B2b of C21, B3I/B2a/B2b of C24 and B2b of C28 among B1I/B1C/B3I/B2a/B2b of C21/C24/C27/C28/C37. The influence of the BDS-3 satellite-induced elevation­dependent code bias on precision positioning and ambiguity fixing is worth further study using different antennas or receivers.

SNR-Dependent Environmental Model: Application in Real-Time GNSS Landslide Monitoring.

The Global Navigation Satellite System (GNSS) is currently one of the important tools for landslide monitoring and early warning. However, the majority of GNSS devices are installed in mountainous areas and a variety of vegetation. These harsh environments lead to defective signals at high elevation angles, rendering real-time successive and reliable positioning results for monitoring difficult. In this study, an environmental model derived from signal-to-noise ratio (SNR) is proposed to enhance the precision and convergence time of positioning in harsh environments. A series of experiments are conducted on weighting and ambiguity-fixed models to evaluate performance. The results indicate that the proposed SNR-dependent environment model could lead to a significant improvement in precision and convergence time; with an obtained root mean squared result on the millimeter level, a convergence time of a few seconds, and utilization which could reach 100%, for continuous and reliable positioning results. These results indicate that the proposed SNR-dependent environment model enhances the performance of GNSS monitoring and early warning to provide continuous and reliable positioning results in real-time.

Improving the Performance of Multi-GNSS Time and Frequency Transfer Using Robust Helmert Variance Component Estimation.

The combination of multiple Global Navigation Satellite Systems (GNSSs) may improve the performance of time and frequency transfers by increasing the number of available satellites and improving the time dilution of precision. However, the receiver clock estimation is easily affected by the inappropriate weight of multi-GNSSs due to the different characteristics of individual GNSS signals as well as the outliers from observations. Thus, we utilised a robust Helmert variance component estimation (RVCE) approach to determine the appropriate weights of different GNSS observations, and to control for the influence of outliers in these observation in multi-GNSS time and frequency transfer. In order to validate the effectiveness of this approach, four time links were employed. Compared to traditional solutions, the mean improvement of smoothed residuals is 3.43% using the RVCE approach. With respect to the frequency stability of the time links, the RVCE solution outperforms the traditional solution, particularly in the short-term, and the mean improvement is markedly high at 14.89%.

Modeling and Assessment of Precise Time Transfer by Using BeiDou Navigation Satellite System Triple-Frequency Signals.

This study proposes two models for precise time transfer using the BeiDou Navigation Satellite System triple-frequency signals: ionosphere-free (IF) combined precise point positioning (PPP) model with two dual-frequency combinations (IF-PPP1) and ionosphere-free combined PPP model with a single triple-frequency combination (IF-PPP2). A dataset with a short baseline (with a common external time frequency) and a long baseline are used for performance assessments. The results show that IF-PPP1 and IF-PPP2 models can both be used for precise time transfer using BeiDou Navigation Satellite System (BDS) triple-frequency signals, and the accuracy and stability of time transfer is the same in both cases, except for a constant system bias caused by the hardware delay of different frequencies, which can be removed by the parameter estimation and prediction with long time datasets or by a priori calibration.

A New Azimuth-Dependent Elevation Weight (ADEW) Model for Real-Time Deformation Monitoring in Complex Environment by Multi-GNSS.

Global navigation satellite systems (GNSS) have provided an excellent way to monitor micro-deformation in real-time. However, at local sites where landslides frequently occur, the environment can include complex surroundings with mountains, dense vegetation, and human settlements, which can severely degrade the accuracy of positioning with the GNSS technique. In this study, we propose an azimuth-dependent elevation weight (ADEW) model using an azimuth-dependent elevation mask (ADEM) to reduce the effects of multipath errors and improve the accuracy of real-time deformation monitoring in such environments. We developed an adaptive fixed-elevation mask to serve as the outlier of low precision observations at lower elevations for the ADEM, and then, we applied the weighted phase observations into the mitigation process for the effects of multipath errors. The real numerical results indicate that the ADEM model performs better than the conventional model, and the average improvements were 18.91% and 34.93% in the horizontal and vertical direction, respectively. The ADEW model further improved upon the ADEM model results by an additional 21.9% and 29.8% in the horizontal and vertical direction, respectively. Therefore, we propose that the ADEW model can significantly mitigate the effects of multipath errors and improve the accuracy of micro-deformation monitoring via GNSS receivers.

A Unified Model for BDS Wide Area and Local Area Augmentation Positioning Based on Raw Observations.

In this study, a unified model for BeiDou Navigation Satellite System (BDS) wide area and local area augmentation positioning based on raw observations has been proposed. Applying this model, both the Real-Time Kinematic (RTK) and Precise Point Positioning (PPP) service can be realized by performing different corrections at the user end. This algorithm was assessed and validated with the BDS data collected at four regional stations from Day of Year (DOY) 080 to 083 of 2016. When the users are located within the local reference network, the fast and high precision RTK service can be achieved using the regional observation corrections, revealing a convergence time of about several seconds and a precision of about 2-3 cm. For the users out of the regional reference network, the global broadcast State-Space Represented (SSR) corrections can be utilized to realize the global PPP service which shows a convergence time of about 25 min for achieving an accuracy of 10 cm. With this unified model, it can not only integrate the Network RTK (NRTK) and PPP into a seamless positioning service, but also recover the ionosphere Vertical Total Electronic Content (VTEC) and Differential Code Bias (DCB) values that are useful for the ionosphere monitoring and modeling.

Evaluation of Fluorescence Imaging with Reflectance Enhancement (FIRE) for Quantifying Enamel Demineralization In vitro.

OBJECTIVES: To assess the ability of fluorescence imaging with reflectance enhancement (FIRE) to quantify early enamel demineralization in vitro. METHODS: A total of 30 bovine enamel specimens were immersed in demineralizing solution to produce artificial caries. Specimens were examined by FIRE, quantitative light-induced fluorescence (QLF), and polarized light microscopy (PLM) at baseline and after 0.5, 1, 2, 4, 8, 24, 48 and 72 h of immersion. Fluorescence loss measured by FIRE and QLF was compared with lesion depth measurements by PLM. RESULTS: Over all time points, measurement of fluorescence loss by FIRE correlated well with the measurement of x0394;F by QLF and of x0394;Q by QLF. Both FIRE and QLF techniques showed significant correlation with PLM. CONCLUSION: FIRE technology may be useful for quantifying enamel demineralization in vitro.

High expression of UCH37 is significantly associated with poor prognosis in human epithelial ovarian cancer.

Ubiquitin carboxyl-terminal hydrolase 37 (UCH37) is a member of deubiquitinating enzymes. It can suppress protein degradation through disassembling polyubiquitin from the distal subunit of the chain. The aim of this study was to assess the value of UCH37 in predicting tumor recurrence after curative resection in epithelial ovarian cancer (EOC) patients. In this study, the expression level of UCH37 in 5 paired EOC and normal tissue was tested by Western blot. And the association of UCH37 expression and prognostic value was analyzed in 100 tumor specimens from EOC patients, who underwent curative resection between 2003 and 2011. We found that UCH37 was up-regulated in most of the tumor tissue and high expression of UCH37 was an independent significant predictor associated with the poor outcome and recurrence of EOC (p=0.0037 and p=0.0042 in overall and disease-free survival, respectively), especially in the advanced stage of EOC (p=0.0106 and p=0.0115 in overall and disease-free survival, respectively), and may become a novel predictor for prognosis of EOC patients after curative resection. Our data suggest for the first time that UCH37 overexpression is associated with advanced tumor progression and poor clinical outcome of EOC patients and may help physicians make informed decisions regarding adjuvant treatment following curative resection.