RESUMEN
Assigning behavioral functions to neural structures has long been a central goal in neuroscience and is a necessary first step toward a circuit-level understanding of how the brain generates behavior. Here, we map the neural substrates of locomotion and social behaviors for Drosophila melanogaster using automated machine-vision and machine-learning techniques. From videos of 400,000 flies, we quantified the behavioral effects of activating 2,204 genetically targeted populations of neurons. We combined a novel quantification of anatomy with our behavioral analysis to create brain-behavior correlation maps, which are shared as browsable web pages and interactive software. Based on these maps, we generated hypotheses of regions of the brain causally related to sensory processing, locomotor control, courtship, aggression, and sleep. Our maps directly specify genetic tools to target these regions, which we used to identify a small population of neurons with a role in the control of walking.
Asunto(s)
Mapeo Encefálico/métodos , Drosophila melanogaster/fisiología , Animales , Conducta Animal , Femenino , Locomoción , Masculino , Programas InformáticosRESUMEN
Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. Here we report the sequencing of the S. oneidensis genome, which consists of a 4,969,803-base pair circular chromosome with 4,758 predicted protein-encoding open reading frames (CDS) and a 161,613-base pair plasmid with 173 CDSs. We identified the first Shewanella lambda-like phage, providing a potential tool for further genome engineering. Genome analysis revealed 39 c-type cytochromes, including 32 previously unidentified in S. oneidensis, and a novel periplasmic [Fe] hydrogenase, which are integral members of the electron transport system. This genome sequence represents a critical step in the elucidation of the pathways for reduction (and bioremediation) of pollutants such as uranium (U) and chromium (Cr), and offers a starting point for defining this organism's complex electron transport systems and metal ion-reducing capabilities.
Asunto(s)
Regulación Bacteriana de la Expresión Génica , Genoma Bacteriano , Análisis de Secuencia de ADN , Análisis de Secuencia de Proteína , Shewanella/genética , Shewanella/metabolismo , Secuencia de Aminoácidos , Biodegradación Ambiental , Respiración de la Célula , Transporte de Electrón , Expresión Génica , Metales/metabolismo , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Compuestos Orgánicos/metabolismo , Oxidación-Reducción , Plásmidos , Proteómica/métodos , Alineación de Secuencia/métodos , Shewanella/clasificación , Shewanella/patogenicidad , Especificidad de la Especie , Contaminantes Químicos del Agua/metabolismo , Purificación del Agua/métodosRESUMEN
The complete genome of the green-sulfur eubacterium Chlorobium tepidum TLS was determined to be a single circular chromosome of 2,154,946 bp. This represents the first genome sequence from the phylum Chlorobia, whose members perform anoxygenic photosynthesis by the reductive tricarboxylic acid cycle. Genome comparisons have identified genes in C. tepidum that are highly conserved among photosynthetic species. Many of these have no assigned function and may play novel roles in photosynthesis or photobiology. Phylogenomic analysis reveals likely duplications of genes involved in biosynthetic pathways for photosynthesis and the metabolism of sulfur and nitrogen as well as strong similarities between metabolic processes in C. tepidum and many Archaeal species.
Asunto(s)
Chlorobi/genética , Chlorobi/metabolismo , Genoma Bacteriano , Dióxido de Carbono/metabolismo , Cromosomas Bacterianos/genética , Ciclo del Ácido Cítrico , Reparación del ADN , Transporte de Electrón , Duplicación de Gen , Modelos Biológicos , Datos de Secuencia Molecular , Nitrógeno/metabolismo , Estrés Oxidativo , Fotosíntesis , Filogenia , Biosíntesis de Proteínas , Pirroles/metabolismo , Azufre/metabolismo , Terpenos/metabolismo , Tetrapirroles , Transcripción GenéticaRESUMEN
Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived from acetate. Here we report the complete genome sequence of an acetate-utilizing methanogen, Methanosarcina acetivorans C2A. Methanosarcineae are the most metabolically diverse methanogens, thrive in a broad range of environments, and are unique among the Archaea in forming complex multicellular structures. This diversity is reflected in the genome of M. acetivorans. At 5,751,492 base pairs it is by far the largest known archaeal genome. The 4524 open reading frames code for a strikingly wide and unanticipated variety of metabolic and cellular capabilities. The presence of novel methyltransferases indicates the likelihood of undiscovered natural energy sources for methanogenesis, whereas the presence of single-subunit carbon monoxide dehydrogenases raises the possibility of nonmethanogenic growth. Although motility has not been observed in any Methanosarcineae, a flagellin gene cluster and two complete chemotaxis gene clusters were identified. The availability of genetic methods, coupled with its physiological and metabolic diversity, makes M. acetivorans a powerful model organism for the study of archaeal biology. [Sequence, data, annotations and analyses are available at http://www-genome.wi.mit.edu/.]