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1.
J Vet Med Educ ; 43(1): 71-9, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26653288

RESUMEN

The aim of the present study was to assess the content and construct validity of the Canine Laparoscopic Simulator (CLS). Forty-two veterinarians were assigned to experienced (n=12), control (n=15), and training (n=15) groups, which were assessed while performing four laparoscopic tasks on the CLS. The initial and final assessments of all tasks were performed blindly by two experienced surgeons using the Global Operative Assessment of Laparoscopic Skills (GOALS) and a task-specific checklist. At the end of the study, the subjects completed an anonymous survey. The experienced group performed all of the tasks faster, with higher GOALS and checklist scores than the training and control groups (p≤.001). In the second assessment, the training group reduced the time needed to complete all of the tasks and obtained significantly higher GOALS and checklist scores than the control group. The participants perceived the CLS and its training program to be positive or very positive. The CLS and its training program demonstrated content and construct validity, supporting the suitability of the simulator for training and teaching and its ability to distinguish the degree of experience in laparoscopic surgery among veterinarians. In addition, face validity showed that the veterinarians fully accepted the CLS's usefulness for learning basic laparoscopic skills.


Asunto(s)
Competencia Clínica , Simulación por Computador , Enfermedades de los Perros/cirugía , Educación en Veterinaria/métodos , Enfermedades Gastrointestinales/veterinaria , Laparoscopía/veterinaria , Enseñanza/métodos , Animales , Enfermedades de los Perros/diagnóstico , Perros , Enfermedades Gastrointestinales/diagnóstico , Enfermedades Gastrointestinales/cirugía , Aprendizaje , Modelos Anatómicos , Veterinarios
2.
J Vet Med Educ ; 41(3): 218-24, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25000884

RESUMEN

Human laparoscopic simulators have been used in medical education for minimally invasive surgery (MIS) in the past years. Simulator-based laparoscopic training has attracted much interest because unique skills have to be learned not only by surgeons in training but also by surgeons in practice. MIS forces the surgeon to adapt to monocular vision and decreased tactile sensation and entails training and improving hand-eye and hand-hand coordination. Those skills require a learning curve that could be overcome gradually with use of simulators. The Canine Laparoscopic Simulator (CLS) for laparoscopic training was developed based on the working and optical space obtained from computed tomography (CT) scan images of three Beagle dogs. Thirty veterinarians (expert group, n=7; novice group, n=23) performed basic laparoscopic exercises in one training session on the CLS. During the performance of the exercises, an experienced laparoscopic veterinarian assessed all the tasks. Afterwards, participants were asked to complete an anonymous survey describing their experience. Most participants expressed positive opinions about the design and usability of the CLS. There were no significant differences between the two groups' opinions. The CLS showed good preliminary acceptance in the basic laparoscopy tasks by veterinarians. They perceived it to be a good training tool, and these results suggest that CLS is an engaging tool for education but still has some limitations inherent in training boxes. Further studies would be needed to establish the validity of training programs performed in the CLS.


Asunto(s)
Simulación por Computador , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/cirugía , Educación en Veterinaria/métodos , Enfermedades Gastrointestinales/veterinaria , Laparoscopía/veterinaria , Enseñanza/métodos , Animales , Perros , Enfermedades Gastrointestinales/diagnóstico , Enfermedades Gastrointestinales/cirugía , Modelos Anatómicos
3.
J Vet Med Educ ; 41(3): 209-17, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24947679

RESUMEN

This article reports on the face, content, and construct validity of a new realistic composite simulator (Simuldog) used to provide training in canine gastrointestinal flexible endoscopy. The basic endoscopic procedures performed on the simulator were esophagogastroduodenoscopy (EGD), gastric biopsy (GB), and gastric foreign body removal (FBR). Construct validity was assessed by comparing the performance of novices (final-year veterinary students and recent graduates without endoscopic experience, n=30) versus experienced subjects (doctors in veterinary medicine who had performed more than 50 clinical upper gastrointestinal endoscopic procedures as a surgeon, n=15). Tasks were scored based on completion time, and specific rating scales were developed to assess performance. Internal consistency and inter-rater agreement were assessed. Face and content validity were determined using a 5-point Likert-type scale questionnaire. The novices needed considerably more time than the experts to perform EGD, GB, and FBR, and their performance scores were significantly lower (p<.010). Inter-rater agreement and the internal validity of the rating scales were good. Face validity was excellent, and both groups agreed that the endoscopy scenarios were very realistic. The experts highly valued the usefulness of Simuldog for veterinary training and as a tool for assessing endoscopic skills. Simuldog is the first validated model specifically developed to be used as a training tool for endoscopy techniques in small animals.


Asunto(s)
Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/cirugía , Educación en Veterinaria/métodos , Endoscopía Gastrointestinal/veterinaria , Enfermedades Gastrointestinales/veterinaria , Animales , Biopsia/veterinaria , Simulación por Computador , Perros , Endoscopía del Sistema Digestivo/veterinaria , Cuerpos Extraños/cirugía , Cuerpos Extraños/veterinaria , Enfermedades Gastrointestinales/diagnóstico , Enfermedades Gastrointestinales/cirugía , Modelos Anatómicos , Estómago/cirugía
4.
Biomedicines ; 12(3)2024 Feb 21.
Artículo en Inglés | MEDLINE | ID: mdl-38540095

RESUMEN

Chronic constipation affects around 20% of the population and there is no efficient solution. This perspective review explores the potential of colonic electric stimulation (CES) using neural implants and methods of bioelectronic medicine as a therapeutic way to treat chronic constipation. The review covers the neurophysiology of colonic peristaltic function, the pathophysiology of chronic constipation, the technical aspects of CES, including stimulation parameters, electrode placement, and neuromodulation target selection, as well as a comprehensive analysis of various animal models highlighting their advantages and limitations in elucidating the mechanistic insights and translational relevance for CES. Finally, the main challenges and trends in CES are discussed.

5.
Ann Vasc Surg ; 27(7): 947-53, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23993110

RESUMEN

BACKGROUND: The latest generation in titanium clip application systems, the AnastoClip Vessel Closure System (VCS; LeMaitre Vascular, Burlington, MA), allows surgeons to perform vascular anastomosis more easily and faster than conventional sutures. This system may become the option of choice for vascular reconstruction in pediatric surgery where, as in the case transplant surgery, decreasing vascular occlusion times may influence outcome. The aim of this study was to determine whether VCS metallic clips would allow shorter anastomosis times than conventional interrupted polypropylene or running polyglycolic acid suturing in end-to-end anastomosis performed in the abdominal cava of young pigs. METHODS: Thirty-two domestic swine, 45 days old, were used for this study. All animals were subjected to an end-to-end anastomosis in the abdominal cava. RESULTS: VCS clips are easier to use for the surgeon, significantly decreasing cross-clamping time in caval anastomosis (VCS 10.33 ± 1.75 min vs. interrupted polypropylene sutures 46.00 ± 6.16 min vs. continuous polyglycolic acid sutures 18.16 ± 1.47 min). CONCLUSIONS: VCS clips significantly decrease the time needed for performing an end-to-end anastomosis in the abdominal cava, decreasing cross-clamping time when compared to interrupted polypropylene or running polyglycolic acid sutures.


Asunto(s)
Implantes Absorbibles , Metales , Instrumentos Quirúrgicos , Técnicas de Sutura/instrumentación , Suturas , Procedimientos Quirúrgicos Vasculares/instrumentación , Venas Cavas/cirugía , Factores de Edad , Anastomosis Quirúrgica , Animales , Constricción , Diseño de Equipo , Masculino , Modelos Animales , Ácido Poliglicólico , Polipropilenos , Sus scrofa , Técnicas de Sutura/efectos adversos , Análisis y Desempeño de Tareas , Factores de Tiempo , Procedimientos Quirúrgicos Vasculares/efectos adversos , Venas Cavas/crecimiento & desarrollo
6.
Cir Esp ; 90(1): 38-44, 2012 Jan.
Artículo en Español | MEDLINE | ID: mdl-22078308

RESUMEN

INTRODUCTION: Our aim is to assess the face and content validities of the physical simulator Simulap(®), as well as the construct validity of its assessment method. MATERIAL AND METHODS: Five novice surgeons (G1) and five experts (G2) performed seven basic tasks and one suturing exercise on Simulap(®), which were assessed through an exam based on mistakes and performance time. Face and content validations were carried out by novice surgeons and expert surgeons, respectively. Both validations consisted of a questionnaire graded on a five-point scale about the Simulap(®) and its tasks. Construct validity of the assessment system was determined by comparing the scores of both groups. RESULTS: Surgeons rated the Simulap(®) simulator and its training program positively, obtaining an average score of 4±1.1 for G1 and of 4.9±0.6 for G2. G2 considered training on Simulap(®) very useful for the training of residents and surgeons, obtaining a maximum score of 5. G2 outperformed G1 in all task scores, with statistically significant differences in the eye-hand coordination (G1: 52.2±6.7 vs. G2: 39.6±6.5; P=.027), dissection (G1: 301.8±100.2 vs. G2: 150.8±66.7; P=.028) and suturing exercises (G1: 258.5±87.0 vs. G2: 108.4±20.2; P=.009). CONCLUSIONS: The assessment method for Simulap(®) is able to distinguish different levels of experience in laparoscopic surgery. Furthermore, this simulator showed a great acceptance by surgeons for the learning of basic skills.


Asunto(s)
Laparoscopía/educación , Modelos Anatómicos , Encuestas y Cuestionarios
7.
Cir Esp ; 90(5): 284-91, 2012 May.
Artículo en Español | MEDLINE | ID: mdl-21703603

RESUMEN

Despite the many advantages that laparoscopic surgery has for patients, it involves a series of risks for the surgeon. These are related to the reduced freedom of movement and forced postures which lead to greater muscle fatigue than with conventional surgery. In laparoscopic surgery there are few references on the introduction of training programs in ergonomics, despite the numerous advantages demonstrated in other disciplines. The application of ergonomic criteria in the surgical field could have great benefits, both for surgeons and patients. In this work we attempt to review the existing literature and our experience to provide the surgeon with some ergonomic guidelines for body stance and positioning of equipment. We also present a training model based on ergonomics which we have introduced into the training activities carried out in our Centre.


Asunto(s)
Ergonomía , Laparoscopía/educación , Enfermedades Profesionales/prevención & control , Humanos , Laparoscopía/métodos , Postura
8.
Ann Vasc Surg ; 23(4): 506-18, 2009.
Artículo en Inglés | MEDLINE | ID: mdl-19375889

RESUMEN

Our aim was to study the physiological repercussions of varying ischemic times during laparoscopic aortic surgery. After quarantine, laparoscopy was performed in 24 healthy pigs, which were randomly allocated to four study groups: group I (n=6), laparoscopic infrarenal abdominal aortic surgery with 30 min of crossclamping; group II (n=6), laparoscopic infrarenal abdominal aortic surgery with 60 min of cross-clamping; group III (n=6), laparoscopic infrarenal abdominal aortic surgery with 120 min of cross-clamping; group IV (n=6), 120 min pneumoperitoneum in the absence of aortic cross-clamping (control group). Hematological, biochemical, hormone, hemodynamic, and ventilatory studies were conducted during and after surgery; and a postoperative neurological evaluation was performed 10 days after surgery. Group III evidenced an increase in arterial blood pressure and heart rate that was significantly higher than those present in the other groups. Significant decreases in pH were observed in groups II and III, whereas no changes in this parameter were seen in groups I and IV. Catecholamine levels during surgery were similar in all groups (a significant [p<0.001] increase in plasmatic adrenaline and noradrenaline was seen immediately after pneumoperitoneum creation in all groups). A positive association was found between the duration of aortic clamping and hormone values at 30 and 60 min after declamping but not after 24hr. A significant increase in the renal resistive index (RRI) and a significant decrease in urine output were evidenced during laparoscopy, with significantly lower RRI values seen in group IV immediately after surgery. Thus, a synergic effect of pneumoperitoneum and aortic cross-clamping was seen in this study. These two factors together cause a hemodynamic compromise, with decreased renal perfusion and acidosis, thus negatively affecting the patient's general state during this type of surgery. Despite being well tolerated in healthy pigs, a laparoscopic aortic cross-clamping time over 60min produces significant hemodynamic, metabolic, and hormonal changes. Careful patient selection is mandatory to avoid any severe complications.


Asunto(s)
Acidosis/etiología , Aorta Abdominal/cirugía , Hemodinámica , Enfermedades Renales/etiología , Laparoscopía/efectos adversos , Neumoperitoneo Artificial/efectos adversos , Acidosis/sangre , Animales , Aorta Abdominal/fisiopatología , Presión Sanguínea , Gasto Cardíaco , Constricción , Epinefrina/sangre , Femenino , Frecuencia Cardíaca , Concentración de Iones de Hidrógeno , Enfermedades Renales/sangre , Enfermedades Renales/fisiopatología , Modelos Animales , Norepinefrina/sangre , Recuperación de la Función , Circulación Renal , Porcinos , Factores de Tiempo , Resistencia Vascular
9.
Actas Urol Esp ; 33(8): 895-901, 2009 Sep.
Artículo en Español | MEDLINE | ID: mdl-19900384

RESUMEN

INTRODUCTION: Despite exhibiting histological differences from the human process, canine hormone-induced benign prostatic hyperplasia (BPH) is still the most widely used animal model for evaluating treatment strategies. OBJECTIVES: The aim of this study is to determine the optimal moment for starting a therapeutic trial in this animal model. MATERIAL AND METHODS: Six male beagle dogs over one year of age were used in this study. All animals received a combination of steroid hormones, namely 17beta-estradiol and 5alpha-androstene 3alpha 17beta-diol, every other day during three (Group 1, n=3) or five months (Group 2, n=3). Transrectal ultrasonographic examinations to measure prostate volume were performed monthly. Animals were euthanized after five months for histological study of their prostates. RESULTS: All animals developed BPH, with prostate volume increasing over time as hormones were administered (r=0,910). All ultrasonographic studies performed up to the third month evidenced a significant increase in prostate volume when compared to the prior ultrasound measurement. A significant decrease in prostate volume was seen in Group 1 once hormone administration was interrupted, whereas Group 2 animals showed a continuing increase in prostate size. Histological examination showed almost no evidence of BPH in Group 1 animals, while Group 2 animals clearly exhibited moderate epithelial hyperplasia. CONCLUSIONS: The administration of a combination of steroid hormones is effective in inducing benign prostatic hyperplasia in canines, but this hyperplasia disappears when hormone treatment is interrupted. In order to be useful for experimental studies, hormones should be administered for at least three months before commencing any treatment, and they should be continued throughout the length of the study..


Asunto(s)
Hiperplasia Prostática/diagnóstico por imagen , Hiperplasia Prostática/patología , Androstanos/administración & dosificación , Animales , Perros , Estradiol/administración & dosificación , Estrógenos/administración & dosificación , Masculino , Hiperplasia Prostática/inducido químicamente , Ultrasonografía
10.
Actas Urol Esp ; 33(1): 76-82, 2009 Jan.
Artículo en Español | MEDLINE | ID: mdl-19462729

RESUMEN

INTRODUCTION: When performing vascular reconstruction in growing elements, specifically in paediatric transplant surgery, where a short vascular occlusion time is mandatory, master and easily handled suturing methods are needed. Thus the present study compares conventional continuous suturing with polypropylene and dexon versus easier and faster to apply titanium clips in heterotopic renal autotransplants. MATERIAL AND METHODS: 24 growing pigs were used for this study. Heterotopic renal autotransplant was performed when the animals were 45-days-old using VCS clips, continous Polypropylene or Dexon suturing when anastomosing the renal artery and vein to the aorta and cava in an end-to-side fashion RESULTS: VCS clips were easy to use for the surgeon, significantly (P < or = 0.001) decreasing the time needed for end-to-side anastomosis of the renal artery to the aorta (clips, 5.31 +/- 0.80 min/Polypropylene, 14.25 +/- 2.25 min/Dexon, 14.37 +/- 2.97 min); and also the time needed for end-to-side anastomosis of the renal veins to the cava (clips, 8.25 +/- 1.98 min/Polypropylene, 16.25 +/- 2.96 min/Dexon, 19.00 +/- 4.50 min). CONCLUSIONS: The use of VCS clips in heterotopic renal autotransplants significantly decreases the time needed for vascular reconstruction, compared to conventional suturing.


Asunto(s)
Trasplante de Riñón , Técnicas de Sutura , Suturas , Titanio , Animales , Masculino , Instrumentos Quirúrgicos , Porcinos , Factores de Tiempo
11.
Arch Esp Urol ; 72(9): 904-914, 2019 Nov.
Artículo en Español | MEDLINE | ID: mdl-31697250

RESUMEN

OBJECTIVES: To describe a roadmap of the most representative milestones and considerations in the validation of surgical simulators, especially those of laparoscopic surgery. And additionally, help determine when in this process a simulator can be considered as validated. METHODS: A non-systematic review was carried out searching terms like simulation, validation, training, assessment, skills and learning curve, as well as providing the experience accumulated by our center. RESULTS: An ideal classical validation process should consist of the following steps: fidelity, verification/calibration/ reliability, subjective and objective strategies. Baseline tests of fidelity and verification/calibration/ technological reliability are not always detailed in the simulation literature. A simulator can be considered validated if, at least, satisfactorily completed any of the two main objective strategies, that is, constructive and/or criterion validity. CONCLUSIONS: The methodologies to validate simulators as useful and reliable for the improvement of psychomotor/ technical skills are widely analyzed, although there is a variety of approaches depending on the scientific reference consulted, not being implemented equally in all works. This apparent arbitrariness should be considered in advance because it can lead the researcher to misunderstandings, especially when the simulator will be regarded as valid.


OBJETIVOS: Describir una hoja de ruta de los hitos y consideraciones más representativos en la validación de simuladores quirúrgicos, especialmente los de cirugía laparoscópica. Y adicionalmente contribuir a determinar en qué momento de este proceso puede considerarse un simulador como validado.MÉTODOS: Se realizó una revisión no sistemática con los términos simulación, validación, formación, entrenamiento, evaluación, habilidades y curva de aprendizaje, además de aportar la experiencia acumulada por nuestro centro. RESULTADOS: Un proceso ideal clásico de validación debería constar de los siguientes pasos: Fidelidad, Verificación/ Calibración/Fiabilidad, estrategias subjetivas y objetivas. Las pruebas de inicio tanto de Fidelidad como de Verificación/Calibración/Fiabilidad tecnológica no siempre están descritas de manera explícita en los trabajos de validación de simuladores. Un simulador puede considerarse validado si al menos ha completado satisfactoriamente una validación de cualquiera de los dos grandes bloques de tipo objetivo, es decir, constructiva y/o de criterio. CONCLUSIONES: Los métodos que permiten validar simuladores como útiles y fiables para la mejora de habilidades de tipo psicomotor/técnico están ampliamente documentados aunque existe cierta variedad de enfoques en función de la referencia científica que se consulte, no aplicándose por igual en todos los trabajos. Esta aparente arbitrariedad debería ser conocida de antemano porque puede llevar al investigador a ciertos equívocos, especialmente a la hora de afirmar cuándo el simulador se considera plenamente validado.


Asunto(s)
Laparoscopía , Entrenamiento Simulado , Competencia Clínica , Simulación por Computador , Humanos , Laparoscopía/métodos , Curva de Aprendizaje , Reproducibilidad de los Resultados
12.
J Am Vet Med Assoc ; 252(7): 839-845, 2018 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-29553901

RESUMEN

OBJECTIVE To compare the usefulness of fresh-frozen canine cadavers (FFCCs) and a validated canine simulator model for training veterinary students in basic gastrointestinal endoscopic procedures. DESIGN Randomized trial. SAMPLE 48 veterinary students in their final year of training. PROCEDURES Students were randomly assigned to receive basic gastrointestinal endoscopic training on a canine simulator or FFCC. All students were trained as assigned in esophagogastroduodenoscopy, endoscopic gastric biopsy, and gastric foreign body removal for 2 h/d for 5 days. They then performed each procedure on a live dog, and procedure completion time and performance ability were compared between groups. Two experienced endoscopists used a validated Likert-type procedural rating scale to rate the students' performance. Students completed a survey to rate their training model. RESULTS No significant differences were identified between groups in quality of performance of the 3 endoscopic procedures on a live dog. Students required significantly less time to complete the procedures on a live dog when trained on an FFCC versus canine simulator. Although both training models were considered equally useful by students, training on the simulator was significantly more stimulating. CONCLUSIONS AND CLINICAL RELEVANCE Students showed the same skill level in performing basic endoscopic procedures on live dogs regardless of the training model used, although students who trained on the FFCC completed these procedures faster than students trained on the canine simulator. Use of the simulator appeared to be a viable alternative to use of FFCCs for veterinary endoscopic training, providing students with a good level of proficiency before performing endoscopic procedures on live dogs.


Asunto(s)
Cadáver , Simulación por Computador , Enfermedades de los Perros , Endoscopía Gastrointestinal , Animales , Perros , Femenino , Humanos , Masculino , Competencia Clínica , Enfermedades de los Perros/cirugía , Educación en Veterinaria , Endoscopía Gastrointestinal/veterinaria , Cuerpos Extraños/cirugía , Cuerpos Extraños/veterinaria , Enfermedades Gastrointestinales/cirugía , Enfermedades Gastrointestinales/veterinaria , Distribución Aleatoria
13.
Arch Esp Urol ; 71(1): 63-72, 2018 Jan.
Artículo en Español | MEDLINE | ID: mdl-29336334

RESUMEN

The lack of globally established standards for learning urological laparoscopy has not prevented laparoscopic techniques from evolution and continuous development. Laparoscopy coexists with robotic surgery today, and in the last decade there have been many techniques that have undergone a boom with the use of a laparoscopic approach (total and partial nephrectomy, pyeloplasty, colposacropexy, etc.).We intend to evaluate the progressive incorporation of different surgical techniques in the laparoscopic learning program and, on the other hand, to analyze the evolution of training programs in urological laparoscopy to bring this type of techniques within the hospital surgical activity. We describe our 30-years experience in different training programs in urological laparoscopy that have been sponsored by the Spanish Association of Urology (AEU), and have undergone several validity studies to assess their capacity in order to evaluate effectively basic and advanced laparoscopic skills. We will also highlight the current and future trend towards training models based on surgical competences where individualized training, accreditation and specialization of tutors is crucial, and where the increase in the use of training and evaluation methods based on the simulation are increasingly common.


Asunto(s)
Laparoscopía/educación , Procedimientos Quirúrgicos Urológicos/educación , Urología/educación , Animales , Modelos Animales , Nefrectomía/métodos , Evaluación de Programas y Proyectos de Salud , España , Factores de Tiempo
14.
Med Oral Patol Oral Cir Bucal ; 12(4): E311-6, 2007 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-17664918

RESUMEN

The use of the microscope as a tool for practising Medicine, especially in surgical specialisations, has been established for decades. The microscope was first used in OdontologyDentistry back to the 1970s and 1980s, and was introduced more widely (although it was still far from being in general use) during 1990s. The purpose of this article is to describe the main applications of the microscope in OdontologyDentistry today, as well as providing odontologists and stomatologists, whether specialists or in general practice, with information about microscopic OdontologyDentistry for better patient care. This work also gives particular importance to matters needed to achieve the necessary manual dexterity to work in a magnified operating field using a surgical microscope (SM).


Asunto(s)
Microcirugia , Procedimientos Quirúrgicos Orales/métodos , Humanos
15.
Anesth Analg ; 102(5): 1333-40, 2006 May.
Artículo en Inglés | MEDLINE | ID: mdl-16632805

RESUMEN

In this study we evaluated, in 10 sevoflurane-anesthetized pigs undergoing abdominal surgery, different techniques for measuring autonomic nervous system (ANS) activity: ANSiscope index, spectral analysis of heart-rate variability, hemodynamic variables, and plasma catecholamines and cortisol levels. Animals underwent a 120-min anesthesia during which unilateral ovariectomy was performed. Cardiovascular and respiratory responses were monitored. ANSiscope indices (ANSindex sympathetic, ANSindex parasympathetic and balANSindex) were used to monitor ANS activity. Spectral analysis was performed using an autoregressive model with a parametric method. The low frequency (LF) and high frequency (HF) components were used to interpret the power spectral density of short-term electrocardiograms (ECGs). The relationship LF/(LF+HF) reflects sympathetic activity, HF/(LF+HF) indicates parasympathetic activity, and the LF/HF ratio gives the predominance of the system. Plasma concentrations of adrenaline, noradrenaline, and cortisol were determined at different times. Correlation (P < 0.01) was found between the balANSindex and adrenaline levels and between LF/HF ratio and plasma adrenaline concentrations. Moreover, a significant (P < 0.01) correlation was found between the balANSindex and LF/HF ratio. However, no correlation was seen between the registered ANSiscope indices and hemodynamic variables. The correlation seen in this study suggests that the balANSindex could be a useful tool to monitor ANS activity during anesthesia and surgery.


Asunto(s)
Anestesia General/métodos , Hemodinámica/fisiología , Ovariectomía , Animales , Sistema Nervioso Autónomo/efectos de los fármacos , Sistema Nervioso Autónomo/fisiología , Presión Sanguínea/efectos de los fármacos , Presión Sanguínea/fisiología , Femenino , Frecuencia Cardíaca/efectos de los fármacos , Frecuencia Cardíaca/fisiología , Hemodinámica/efectos de los fármacos , Hidrocortisona/sangre , Éteres Metílicos/administración & dosificación , Sevoflurano , Porcinos
16.
J Invest Surg ; 19(2): 97-104, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16531367

RESUMEN

The purpose of this work was to develop an abdominal aortic aneurysm (AAA) model that resembles human aneurysms with potential for further growth, patent collateral vessels, and a predictable tendency to rupture, and that can be used in the development of new endoprostheses and implant training. An infrarenal AAA model was created in five domestic swine using an autologous gastric serosal patch. Pre- and postsurgical digital subtraction aortograms (DSA) were obtained to document the appearance and dimensions of the aneurysm. Animals were followed up with DSA and ultrasonography on days 7, 14, 30, 45, 60, and 90 after model creation. Aneurysmal diameters were measured with both techniques in all examinations. On day 90, animals were euthanized, target arteries were harvested, and pathological evaluation was performed. The nonparametric Wilcoxon test was used to assess any differences in measured diameters. All the animals survived the surgical procedure. The aneurysmal diameters increased from 8.14+/- 2.15 to 13.28+/- 1.18 mm immediately after surgery (p < .05), but no subsequent significant growth of the aneurysmal sac was seen during follow-up. In this experimental setting, measurements obtained with DSA were slightly larger than those obtained with ultrasound. Two animals died of AAA rupture on days 6 and 10 (40% rupture rate). Pathological examination showed lack of elastic laminae and increased collagen content in the aortic patch. Thus, model showed a tendency to rupture, but no significant potential for further aneurysmal growth. It might be useful for training in endovascular therapies, but its usefulness for preclinical endovascular device testing is limited by its lack of growth potential.


Asunto(s)
Aneurisma de la Aorta Abdominal , Procedimientos Quirúrgicos Cardiovasculares/métodos , Modelos Animales de Enfermedad , Animales , Aneurisma de la Aorta Abdominal/diagnóstico por imagen , Aneurisma de la Aorta Abdominal/patología , Aneurisma de la Aorta Abdominal/cirugía , Rotura de la Aorta , Aortografía , Educación de Postgrado en Medicina , Membrana Serosa/cirugía , Estómago/cirugía , Porcinos , Ultrasonografía
17.
Res Vet Sci ; 81(3): 373-81, 2006 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-16516255

RESUMEN

The objective of this study is to evaluate the usefulness of bispectral index (BIS), spectral edge frequency 95% (SEF) and median frequency (MED) in relation to a simple descriptive scale (SDS) as indicators of anaesthetic depth at different desflurane concentrations in swine. Sixteen pigs were randomly allocated to four groups. Electroencephalograms (EEG) were recorded during desflurane anaesthesia, and BIS, SEF and MED were calculated from the EEG. The agent was administered in pure oxygen at 1, 1.25, 1.5 and 1.7 MAC in randomized order. Anaesthetic depth was evaluated on a SDS. BIS decreased significantly (P<0.001) at the different anaesthetic dosages used. SEF decreased significantly (P<0.001) from basal to 1 MAC of desflurane. MED decreased significantly (P<0.001) from basal to 1 MAC and from 1 to 1.75 MAC. Good correlation was seen between SDS scores and BIS values and between SDS scores and MED values. BIS appeared to be useful to predict changes in anaesthetic depth at clinically used dosages of inhalant anaesthesia.


Asunto(s)
Anestesia/veterinaria , Anestésicos por Inhalación/farmacología , Electroencefalografía/veterinaria , Isoflurano/análogos & derivados , Porcinos , Animales , Desflurano , Relación Dosis-Respuesta a Droga , Electroencefalografía/métodos , Femenino , Isoflurano/farmacología , Masculino
18.
Lab Anim (NY) ; 45(2): 67-74, 2016 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-26814353

RESUMEN

Cardiovascular diseases are a major health concern and therefore an important topic in biomedical research. Large animal models allow researchers to assess the safety and efficacy of new cardiovascular procedures in systems that resemble human anatomy; additionally, they can be used to emulate scenarios for training purposes. Among the many biomedical models that are described in published literature, it is important that researchers understand and select those that are best suited to achieve the aims of their research, that facilitate the humane care and management of their research animals and that best promote the high ethical standards required of animal research. In this resource the authors describe some common swine models that can be easily incorporated into regular practices of research and training at biomedical institutions. These models use both native and altered vascular anatomy of swine to carry out research protocols, such as testing biological reactions to implanted materials, surgically creating aneurysms using autologous tissue and inducing myocardial infarction through closed-chest procedures. Such models can also be used for training, where native and altered vascular anatomy allow medical professionals to learn and practice challenging techniques in anatomy that closely simulates human systems.


Asunto(s)
Enfermedades Cardiovasculares/diagnóstico , Enfermedades Cardiovasculares/terapia , Modelos Animales de Enfermedad , Sus scrofa , Animales , Investigación Biomédica/educación , Enfermedades Cardiovasculares/etiología
19.
Am J Vet Res ; 77(2): 186-93, 2016 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27027713

RESUMEN

OBJECTIVE: To evaluate muscle activity and hand motion in veterinarians performing a standard set of laparoscopic training tasks. SAMPLE: 12 veterinarians with experience performing laparoscopic procedures. PROCEDURES: Participants were asked to perform peg transfer, coordination, precision cutting, and suturing tasks in a laparoscopic box trainer. Activity of the right biceps brachii, triceps brachii, forearm flexor, forearm extensor, and trapezius muscles was analyzed by means of surface electromyography. Right hand movements and wrist angle data were registered through the use of a data glove, and risk levels for the wrist joint were determined by use of a modified rapid upper limb assessment (RULA) method. One-way repeated-measures ANOVA with a Bonferroni post hoc test was performed to compare values between tasks. RESULTS: Activity in the biceps muscle did not differ significantly among the 4 tasks. Activity in the triceps, forearm flexor, and forearm extensor muscles was significantly higher during precision cutting than during the coordination task. Activity in the trapezius muscle was highest during the suturing task and did not differ significantly among the other 3 tasks. The RULA score was unacceptable (score, 3) for the coordination, peg transfer, and precision cutting tasks but was acceptable (score, 2) for the suturing task. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that the ergonomics of laparoscopic training depended on the tasks performed and the design of the instruments used. Precision cutting and suturing tasks were associated with the highest muscle activity. Acceptable wrist position, as determined with the RULA method, was found with the suturing task, which was performed with an axial-handled instrument.


Asunto(s)
Educación en Veterinaria/métodos , Ergonomía , Laparoscopía/veterinaria , Veterinarios , Brazo , Humanos , Laparoscopía/educación , Movimiento
20.
Am J Vet Res ; 64(9): 1099-104, 2003 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-13677386

RESUMEN

OBJECTIVE: To evaluate the use of ultrasonography to detect morphologic changes in the pylorus during pyloroplasty performed laparoscopically or via conventional abdominal surgery in dogs. ANIMALS: 10 healthy mixed-breed dogs. PROCEDURE: Laparoscopic ultrasonography of the pylorus was performed in 5 dogs during laparoscopic pyloroplasty (LP), and ultrasonography of the pylorus was performed in 5 dogs during pyloroplasty via conventional abdominal surgery (CAP group). Appearance and dimensions of the pyloric sphincter were evaluated by use of a 7.5-MHz flexible laparoscopic linear-transducer probe. RESULTS: Mean +/- SD duration of the ultrasonographic procedure was 11 +/- 3.04 minutes (range, 6 to 18 minutes). In the CAP group, cross-sectional views of the pylorus revealed significant differences between the overall transverse external diameter, overall craniocaudal external diameter, and transverse diameter of the pyloric lumen. After surgery, the pyloric area was significantly increased. Longitudinal views of the pylorus revealed that width of the pyloric ring was significantly less after surgery. Transverse views of the pylorus for the LP group revealed a significant increase in the transverse diameter and craniocaudal diameter of the pyloric lumen after LP. The pyloric area was also significantly increased after surgery. Longitudinal views of the pylorus revealed that width of the pyloric ring was significantly less after surgery. Transverse diameter of the pyloric lumen was significantly increased after LP. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of results of this study suggests that ultrasonography is useful for detecting relevant morphologic changes in the pyloric sphincter after pyloroplasty.


Asunto(s)
Perros/anatomía & histología , Perros/cirugía , Píloro/diagnóstico por imagen , Píloro/cirugía , Animales , Femenino , Laparoscopía/métodos , Laparoscopía/veterinaria , Masculino , Distribución Aleatoria , Ultrasonografía Doppler en Color/veterinaria
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