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1.
Cell ; 152(4): 667-8, 2013 Feb 14.
Artículo en Inglés | MEDLINE | ID: mdl-23415216

RESUMEN

One of the strongest signals of positive selection in humans surrounds the V370A variant of Ectodysplasin A receptor (EDAR). However, its phenotypic consequences and impetus for selection are not well understood. Kamberov et al. nail down when it originated and, using transgenic mice, delineate its phenotypic impacts.

2.
Nature ; 505(7481): 43-9, 2014 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-24352235

RESUMEN

We present a high-quality genome sequence of a Neanderthal woman from Siberia. We show that her parents were related at the level of half-siblings and that mating among close relatives was common among her recent ancestors. We also sequenced the genome of a Neanderthal from the Caucasus to low coverage. An analysis of the relationships and population history of available archaic genomes and 25 present-day human genomes shows that several gene flow events occurred among Neanderthals, Denisovans and early modern humans, possibly including gene flow into Denisovans from an unknown archaic group. Thus, interbreeding, albeit of low magnitude, occurred among many hominin groups in the Late Pleistocene. In addition, the high-quality Neanderthal genome allows us to establish a definitive list of substitutions that became fixed in modern humans after their separation from the ancestors of Neanderthals and Denisovans.


Asunto(s)
Fósiles , Genoma/genética , Hombre de Neandertal/genética , África , Animales , Cuevas , Variaciones en el Número de Copia de ADN/genética , Femenino , Flujo Génico/genética , Frecuencia de los Genes , Heterocigoto , Humanos , Endogamia , Modelos Genéticos , Hombre de Neandertal/clasificación , Filogenia , Densidad de Población , Siberia/etnología , Falanges de los Dedos del Pie/anatomía & histología
3.
Mol Biol Evol ; 35(5): 1190-1209, 2018 05 01.
Artículo en Inglés | MEDLINE | ID: mdl-29688543

RESUMEN

Pigmentation is often used to understand how natural selection affects genetic variation in wild populations since it can have a simple genetic basis, and can affect a variety of fitness-related traits (e.g., camouflage, thermoregulation, and sexual display). In gray wolves, the K locus, a ß-defensin gene, causes black coat color via a dominantly inherited KB allele. The allele is derived from dog-wolf hybridization and is at high frequency in North American wolf populations. We designed a DNA capture array to probe the geographic origin, age, and number of introgression events of the KB allele in a panel of 331 wolves and 20 dogs. We found low diversity in KB, but not ancestral ky, wolf haplotypes consistent with a selective sweep of the black haplotype across North America. Further, North American wolf KB haplotypes are monophyletic, suggesting that a single adaptive introgression from dogs to wolves most likely occurred in the Northwest Territories or Yukon. We use a new analytical approach to date the origin of the KB allele in Yukon wolves to between 1,598 and 7,248 years ago, suggesting that introgression with early Native American dogs was the source. Using population genetic simulations, we show that the K locus is undergoing natural selection in four wolf populations. We find evidence for balancing selection, specifically in Yellowstone wolves, which could be a result of selection for enhanced immunity in response to distemper. With these data, we demonstrate how the spread of an adaptive variant may have occurred across a species' geographic range.


Asunto(s)
Color del Cabello/genética , Selección Genética , Lobos/genética , beta-Defensinas/genética , Animales , Simulación por Computador , Perros , Frecuencia de los Genes , Variación Genética , Haplotipos , Homocigoto , América del Norte
4.
BMC Genomics ; 16: 1034, 2015 Dec 07.
Artículo en Inglés | MEDLINE | ID: mdl-26643904

RESUMEN

BACKGROUND: Determining whether two DNA samples originate from the same individual is difficult when the amount of retrievable DNA is limited. This is often the case for ancient, historic, and forensic samples. The most widely used approaches rely on amplification of a defined panel of multi-allelic markers and comparison to similar data from other samples. When the amount retrievable DNA is low these approaches fail. RESULTS: We describe a new method for assessing whether shotgun DNA sequence data from two samples are consistent with originating from the same or different individuals. Our approach makes use of the large catalogs of single nucleotide polymorphism (SNP) markers to maximize the chances of observing potentially discriminating alleles. We further reduce the amount of data required by taking advantage of patterns of linkage disequilibrium modeled by a reference panel of haplotypes to indirectly compare observations at pairs of linked SNPs. Using both coalescent simulations and real sequencing data from modern and ancient sources, we show that this approach is robust with respect to the reference panel and has power to detect positive identity from DNA libraries with less than 1 % random and non-overlapping genome coverage in each sample. CONCLUSION: We present a powerful new approach that can determine whether DNA from two samples originated from the same individual even when only minute quantities of DNA are recoverable from each.


Asunto(s)
Genética Forense/métodos , Técnicas de Diagnóstico Molecular , Análisis de Secuencia de ADN/métodos , Alelos , Simulación por Computador , Sitios Genéticos , Biblioteca Genómica , Humanos , Desequilibrio de Ligamiento , Polimorfismo de Nucleótido Simple
5.
Elife ; 122024 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-38488477

RESUMEN

Ancient environmental DNA (aeDNA) from lake sediments has yielded remarkable insights for the reconstruction of past ecosystems, including suggestions of late survival of extinct species. However, translocation and lateral inflow of DNA in sediments can potentially distort the stratigraphic signal of the DNA. Using three different approaches on two short lake sediment cores of the Yamal peninsula, West Siberia, with ages spanning only the past hundreds of years, we detect DNA and identified mitochondrial genomes of multiple mammoth and woolly rhinoceros individuals-both species that have been extinct for thousands of years on the mainland. The occurrence of clearly identifiable aeDNA of extinct Pleistocene megafauna (e.g. >400 K reads in one core) throughout these two short subsurface cores, along with specificities of sedimentology and dating, confirm that processes acting on regional scales, such as extensive permafrost thawing, can influence the aeDNA record and should be accounted for in aeDNA paleoecology.


Asunto(s)
Genoma Mitocondrial , Humanos , Lagos , Ecosistema , ADN , Análisis de Secuencia de ADN , ADN Antiguo
6.
PLoS Comput Biol ; 6(4): e1000732, 2010 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-20369015

RESUMEN

In bacterial genomes composed of more than one chromosome, one replicon is typically larger, harbors more essential genes than the others, and is considered primary. The greater variability of secondary chromosomes among related taxa has led to the theory that they serve as an accessory genome for specific niches or conditions. By this rationale, purifying selection should be weaker on genes on secondary chromosomes because of their reduced necessity or usage. To test this hypothesis we selected bacterial genomes composed of multiple chromosomes from two genera, Burkholderia and Vibrio, and quantified the evolutionary rates (dN and dS) of all orthologs within each genus. Both evolutionary rate parameters were faster among orthologs found on secondary chromosomes than those on the primary chromosome. Further, in every bacterial genome with multiple chromosomes that we studied, genes on secondary chromosomes exhibited significantly weaker codon usage bias than those on primary chromosomes. Faster evolution and reduced codon bias could in turn result from global effects of chromosome position, as genes on secondary chromosomes experience reduced dosage and expression due to their delayed replication, or selection on specific gene attributes. These alternatives were evaluated using orthologs common to genomes with multiple chromosomes and genomes with single chromosomes. Analysis of these ortholog sets suggested that inherently fast-evolving genes tend to be sorted to secondary chromosomes when they arise; however, prolonged evolution on a secondary chromosome further accelerated substitution rates. In summary, secondary chromosomes in bacteria are evolutionary test beds where genes are weakly preserved and evolve more rapidly, likely because they are used less frequently.


Asunto(s)
Evolución Molecular , Genoma Bacteriano , Bacterias Gramnegativas/genética , Modelos Genéticos , Burkholderia/genética , Codón , Mutación , Filogenia , ARN Ribosómico 16S/genética , Vibrio/genética , Xanthomonas/genética
7.
Science ; 361(6401): 511-516, 2018 08 03.
Artículo en Inglés | MEDLINE | ID: mdl-30072539

RESUMEN

Flores Island, Indonesia, was inhabited by the small-bodied hominin species Homo floresiensis, which has an unknown evolutionary relationship to modern humans. This island is also home to an extant human pygmy population. Here we describe genome-scale single-nucleotide polymorphism data and whole-genome sequences from a contemporary human pygmy population living on Flores near the cave where H. floresiensis was found. The genomes of Flores pygmies reveal a complex history of admixture with Denisovans and Neanderthals but no evidence for gene flow with other archaic hominins. Modern individuals bear the signatures of recent positive selection encompassing the FADS (fatty acid desaturase) gene cluster, likely related to diet, and polygenic selection acting on standing variation that contributed to their short-stature phenotype. Thus, multiple independent instances of hominin insular dwarfism occurred on Flores.


Asunto(s)
Adaptación Biológica/genética , Evolución Biológica , Estatura/genética , Enanismo/genética , Islas , Población/genética , Selección Genética , Animales , Flujo Génico , Genoma Humano , Humanos , Indonesia , Hombre de Neandertal/genética
8.
Forensic Sci Int Genet ; 30: 93-105, 2017 09.
Artículo en Inglés | MEDLINE | ID: mdl-28667863

RESUMEN

Massively parallel (next-generation) sequencing provides a powerful method to analyze DNA from many different sources, including degraded and trace samples. A common challenge, however, is that many forensic samples are often known or suspected mixtures of DNA from multiple individuals. Haploid lineage markers, such as mitochondrial (mt) DNA, are useful for analysis of mixtures because, unlike nuclear genetic markers, each individual contributes a single sequence to the mixture. Deconvolution of these mixtures into the constituent mitochondrial haplotypes is challenging as typical sequence read lengths are too short to reconstruct the distinct haplotypes completely. We present a powerful computational approach for determining the constituent haplotypes in massively parallel sequencing data from potentially mixed samples. At the heart of our approach is an expectation maximization based algorithm that co-estimates the overall mixture proportions and the source haplogroup for each read individually. This approach, implemented in the software package mixemt, correctly identifies haplogroups from mixed samples across a range of mixture proportions. Furthermore, our method can separate fragments in a mixed sample by the most likely originating contributor and generate reconstructions of the constituent haplotypes based on known patterns of mtDNA diversity.


Asunto(s)
ADN Mitocondrial/genética , Haplotipos , Secuenciación de Nucleótidos de Alto Rendimiento , Filogenia , Análisis de Secuencia de ADN , Algoritmos , Humanos , Grupos Raciales/genética
9.
Science ; 358(6365): 951-954, 2017 11 17.
Artículo en Inglés | MEDLINE | ID: mdl-29146814

RESUMEN

The extinct passenger pigeon was once the most abundant bird in North America, and possibly the world. Although theory predicts that large populations will be more genetically diverse, passenger pigeon genetic diversity was surprisingly low. To investigate this disconnect, we analyzed 41 mitochondrial and 4 nuclear genomes from passenger pigeons and 2 genomes from band-tailed pigeons, which are passenger pigeons' closest living relatives. Passenger pigeons' large population size appears to have allowed for faster adaptive evolution and removal of harmful mutations, driving a huge loss in their neutral genetic diversity. These results demonstrate the effect that selection can have on a vertebrate genome and contradict results that suggested that population instability contributed to this species's surprisingly rapid extinction.


Asunto(s)
Columbidae/genética , Extinción Biológica , Variación Genética , Selección Genética , Animales , Núcleo Celular/genética , Genes Mitocondriales/genética , Genómica , Mutación , América del Norte , Densidad de Población
10.
Nat Genet ; 48(9): 1089-93, 2016 09.
Artículo en Inglés | MEDLINE | ID: mdl-27428749

RESUMEN

The cereal grass barley was domesticated about 10,000 years before the present in the Fertile Crescent and became a founder crop of Neolithic agriculture. Here we report the genome sequences of five 6,000-year-old barley grains excavated at a cave in the Judean Desert close to the Dead Sea. Comparison to whole-exome sequence data from a diversity panel of present-day barley accessions showed the close affinity of ancient samples to extant landraces from the Southern Levant and Egypt, consistent with a proposed origin of domesticated barley in the Upper Jordan Valley. Our findings suggest that barley landraces grown in present-day Israel have not experienced major lineage turnover over the past six millennia, although there is evidence for gene flow between cultivated and sympatric wild populations. We demonstrate the usefulness of ancient genomes from desiccated archaeobotanical remains in informing research into the origin, early domestication and subsequent migration of crop species.


Asunto(s)
Aclimatación/genética , Domesticación , Genes de Plantas/genética , Genética de Población , Estudio de Asociación del Genoma Completo , Genómica/métodos , Hordeum/genética , Flujo Génico , Genoma de Planta , Polimorfismo de Nucleótido Simple/genética
12.
Genome Biol Evol ; 2: 859-69, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-20978102

RESUMEN

In bacterial chromosomes, the position of a gene relative to the single origin of replication generally reflects its replication timing, how often it is expressed, and consequently, its rate of evolution. However, because some archaeal genomes contain multiple origins of replication, bias in gene dosage caused by delayed replication should be minimized and hence the substitution rate of genes should associate less with chromosome position. To test this hypothesis, six archaeal genomes from the genus Sulfolobus containing three origins of replication were selected, conserved orthologs were identified, and the evolutionary rates (dN and dS) of these orthologs were quantified. Ortholog families were grouped by their consensus position and designated by their proximity to one of the three origins (O1, O2, O3). Conserved orthologs were concentrated near the origins and most variation in genome content occurred distant from the origins. Linear regressions of both synonymous and nonsynonymous substitution rates on distance from replication origins were significantly positive, the rates being greatest in the region furthest from any of the origins and slowest among genes near the origins. Genes near O1 also evolved faster than those near O2 and O3, which suggest that this origin may fire later in the cell cycle. Increased evolutionary rates and gene dispensability are strongly associated with reduced gene expression caused in part by reduced gene dosage during the cell cycle. Therefore, in this genus of Archaea as well as in many Bacteria, evolutionary rates and variation in genome content associate with replication timing.


Asunto(s)
Momento de Replicación del ADN , Evolución Molecular , Sulfolobus/genética , Composición de Base , Posicionamiento de Cromosoma , Codón , Secuencia Conservada , Bases de Datos Genéticas , Dosificación de Gen , Perfilación de la Expresión Génica , Regulación de la Expresión Génica Arqueal , Orden Génico , Genes Arqueales , Genoma Arqueal , Recombinación Genética , Origen de Réplica , Análisis de Secuencia de ADN , Análisis de Secuencia de Proteína , Homología de Secuencia de Ácido Nucleico , Sulfolobus/metabolismo , Sulfolobus solfataricus/genética , Sulfolobus solfataricus/metabolismo
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