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In this work, a new pyrylium derivatization-assisted liquid chromatography-mass spectrometry (LC-MS) method was developed for metabolite profiling of the glutathione anabolic pathway (GAP) in cancer tissues and cells. The pyrylium salt of 6,7-dimethoxy-3-methyl isochromenylium tetrafluoroborate (DMMIC) was used to label the amino group of metabolites, and a reductant of dithiothreitol (DTT) was employed to stabilize the thiol group. By combining DMMIC derivatization with LC-MS, it was feasible to quantify the 13 main metabolites on the GAP in complex biological samples, which had good linearity (R2 = 0.9981-0.9999), precision (interday precision of 1.6%-19.0% and intraday precision of 1.4%-19.8%) and accuracy (83.4%-115.7%). Moreover, the recovery assessments in tissues (82.5%-107.3%) and in cells (98.1%-118.9%) with GSH-13C2, 15N, and Cys-15N demonstrated the reliability of the method in detecting tissues and cells. Following a methodological evaluation, the method was applied successfully to investigate difference in the GAP between the carcinoma and para-carcinoma tissues of esophageal squamous cell carcinoma (ESCC) and the effect of p-hydroxycinnamaldehyde (CMSP) on the GAP in KYSE-150 esophageal cancer cells. The results demonstrate that the developed method provides a promising new tool to elucidate the roles of GAP in physiological and pathological processes, which can contribute to research on drugs and diseases.
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Background: This study sought to implement evidence-based nursing practices for patients undergoing laparoscopy surgery to prevent unplanned hypothermia, to develop review indicators for evidence-based nursing practices, and to analyze obstacles and contributing factors. Methods: Using the Joanna Briggs Institute health care model as the evidence-based theoretical framework, clinical problems were identified, evidence was systematically researched, evaluated and summarized, an index and method of examination was established, and quality items were examined. According to the results of the baseline review, the obstacles and promoting factors were analyzed, and methods and countermeasures were developed. Results: This study summarized 15 pieces of best evidence in relation to the following 5 aspects: risk assessment, body temperature monitoring, ambient temperature, passive insulation, and active insulation. There were 11 quality review indicators, only 4 of which had compliance rates of 100%. The main obstacle factors were a lack of nursing norms and operating procedures, a lack of information, and a lack of motivation among nursing staff. Conclusions: Based on the best evidence and the professional judgment of clinical staff, the quality review index is scientific and implementable. In the application of this clinical evidence, obstacles and other factors should be solved to promote the transformation of evidence.
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BACKGROUND: This study aimed to search, analyze, and summarize relevant evidence for the prevention of unplanned hypothermia in patients undergoing laparoscopic surgery, so as to reduce the incidence of unplanned hypothermia in patients undergoing laparoscopic surgery and provide a reference for clinical medical staff. METHODS: According to the evidence pyramid model, relevant literature were retrieved by computer in databases. Literature quality evaluation and the evidence grading system of the Australian JBI Evidence-Based Health Care Centre Evidence Recommendation Rating System were used to evaluate the literature quality and determine the level of evidence. The time limit for retrieval was from the establishment of the databases to March 31, 2021. RESULTS: A total of 12 studies were included in this study. Of these, 5 evidence items were finally extracted, and 15 pieces of best evidence were summarized, including the risk assessment of hypothermia in patients undergoing laparoscopic surgery, temperature monitoring, ambient temperature, passive insulation measures, and active insulation measures. CONCLUSIONS: The evidence for the prevention of unplanned hypothermia in patients undergoing laparoscopic surgery provided evidence-based approaches for reducing the incidence of intraoperative hypothermia for clinical staffs. It is suggested that the cultural characteristics of China, medical resources, and patients' own conditions should be considered when applying the evidence.
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Spinal cord injury can lead to devastating and permanent loss of neurological function, affecting all levels below the site of trauma. Unfortunately, the injured adult mammalian spinal cord displays little regenerative capacity and little functional recovery in large part due to a tissue environment that is nonpermissive for regenerative axon growth. Artificial tissue repair scaffolds may provide a physical guide to allow regenerative axon growth that bridges the lesion cavity and restores functional neural connectivity. By integrating different strategies, including the use of various biomaterials and microstructures as well as incorporation of bioactive molecules and living cells, combined or synergistic effects for spinal cord repair through regenerative axon growth may be achieved. This article briefly reviews the development of bioengineered scaffolds for spinal cord repair, focusing on spinal cord injury and the subsequent cellular response, scaffold materials, fabrication techniques, and current therapeutic strategies. Key issues and challenges are also identified and discussed along with recommendations for future research.