RESUMEN
BACKGROUND: The urine protein/creatinine ratio (UPCR) is commonly used in current clinical practice. However, there are only few published clinical data on UPCR from large cohorts of Chinese adults. This study aimed to determine the overall and age- and sex-specific UPCR reference values for healthy Dalian adults. METHODS: According to the Clinical & Laboratory Standards Institute EP28-A3c guidelines, 1321 healthy Dalian adults (646 men and 675 women) aged 20-69 years were enrolled. Urine protein and creatinine levels were analyzed in the random morning spot urine samples, and UPCR was calculated. The 95th percentile of the UPCR was used as the normal upper limit. The Mann-Whitney U test was used to test differences among groups. RESULTS: The UPCR reference value was 141.7 mg/g for the entire cohort, 128.7 mg/g for men, and 150.8 mg/g for women. In addition, women had relatively higher UPCR values than men in the same age group. We also compared the UPCR reference values between different estimated glomerular filtration rate (eGFR) groups and found that women had significantly higher UPCR values than men in the normal eGFR groups. CONCLUSIONS: This study provides the overall and age- and sex-specific UPCR reference values for healthy Dalian adults.
Asunto(s)
Creatinina/orina , Pruebas de Función Renal/normas , Proteinuria/orina , Urinálisis/normas , Adulto , Anciano , China , Humanos , Persona de Mediana Edad , Valores de Referencia , Adulto JovenRESUMEN
The expression pattern, functions, and detailed regulatory mechanisms of miR-379 in laryngeal carcinoma remain unknown. In the present study, we aimed to uncover novel potential miR-379 targets and shed light on its roles in laryngeal carcinoma. The expression level of miR-379 was measured based on the data obtained from the TCGA database and the cell lines. After miR-379 mimic transfection, cell proliferation, invasion and migration assay, and wound-healing assay in HEp-2 cell line were implemented to evaluate the effects of miR-379 on laryngeal carcinoma in vitro. The target genes for miR-379 in silico were predicted and validated using luciferase reporter assay. The miR-379 expression level was reduced in laryngeal carcinoma tissues and cell lines. Moreover, miR-379 overexpression inhibited the cell proliferation, migration, and invasion of laryngeal carcinoma cells. TCF-4 was detected as a direct target of miR-379 in laryngeal carcinoma. Furthermore, restored TCF-4 expression rescued the inhibitory roles of miR-379 overexpression on cell proliferation, migration, and invasion.Our study for the first time demonstrated that miR-379/TCF-4 might involve in the progression of laryngeal carcinoma, and miR-379 appears to serve as a novel tumor suppressor in laryngeal carcinoma.
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Neoplasias Laríngeas/metabolismo , MicroARNs/metabolismo , Proteína 2 Similar al Factor de Transcripción 7/metabolismo , Western Blotting , Línea Celular , Línea Celular Tumoral , Movimiento Celular/efectos de los fármacos , Movimiento Celular/genética , Proliferación Celular/efectos de los fármacos , Proliferación Celular/genética , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/genética , Regulación Neoplásica de la Expresión Génica/genética , Humanos , Neoplasias Laríngeas/genética , Neoplasias Laríngeas/patología , Lípidos/farmacología , MicroARNs/genética , Invasividad Neoplásica/genética , Invasividad Neoplásica/fisiopatología , Proteína 2 Similar al Factor de Transcripción 7/genéticaRESUMEN
Sow milk yield and quality is crucial for the survival and growth of piglets. To understand the molecular mechanisms of lactogenesis and lactation, mammary tissue samples were taken from six sows at -17(±2), 1 and 17(±2) days relative to parturition. Mammary tissues from two sows in the same stage were used to extract RNA, which were subsequently pooled in equal amounts. Nine pooled samples were hybridized to porcine Affymetrix GeneChips. Totally 1,524 genes were detected as significantly differentially expressed over the time course tested (p<0.01, q<0.01, fold change≥2 or ≤-2), including 709 upregulated and 575 downregulated genes identified at peak lactation compared to late pregnancy. Gene ontology analysis revealed that most of the upregulated genes were involved in transport, biosynthetic processes, and homeostasis, whereas most of the downregulated genes were involved in intracellular signaling cascades, cell cycle, and DNA replication. Furthermore, we identified 64 differentially expressed genes of the solute carrier families. Taken together, our microarray analysis provides insights into previously uncharacterized changes in transcriptome between late pregnancy and peak lactation in the porcine mammary gland. The solute carrier genes and other differentially expressed genes identified in this study will guide further characterization of their function to enhance milk yield and piglet growth.