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1.
J Gen Intern Med ; 39(5): 731-738, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38302813

RESUMEN

BACKGROUND: Experts estimate virtual urgent care programs could replace approximately 20% of current emergency department visits. In the absence of widespread quality guidance to programs or quality reporting from these programs, little is known about the state of virtual urgent care quality monitoring initiatives. OBJECTIVE: We sought to characterize ongoing quality monitoring initiatives among virtual urgent care programs. APPROACH: Semi-structured interviews of virtual health and health system leaders were conducted using a pilot-tested interview guide to assess quality metrics captured related to care effectiveness and equity as well as programs' motivations for and barriers to quality measurement. We classified quality metrics according to the National Quality Forum Telehealth Measurement Framework. We developed a codebook from interview transcripts for qualitative analysis to classify motivations for and barriers to quality measurement. KEY RESULTS: We contacted 13 individuals, and ultimately interviewed eight (response rate, 61.5%), representing eight unique virtual urgent care programs at primarily academic (6/8) and urban institutions (5/8). Most programs used quality metrics related to clinical and operational effectiveness (7/8). Only one program reported measuring a metric related to equity. Limited resources were most commonly cited by participants (6/8) as a barrier to quality monitoring. CONCLUSIONS: We identified variation in quality measurement use and content by virtual urgent care programs. With the rapid growth in this approach to care delivery, more work is needed to identify optimal quality metrics. A standardized approach to quality measurement will be key to identifying variation in care and help focus quality improvement by virtual urgent care programs.


Asunto(s)
Telemedicina , Humanos , Telemedicina/normas , Telemedicina/métodos , Atención Ambulatoria/normas , Calidad de la Atención de Salud/normas , Motivación , Indicadores de Calidad de la Atención de Salud
2.
J Med Internet Res ; 25: e42335, 2023 03 16.
Artículo en Inglés | MEDLINE | ID: mdl-36928088

RESUMEN

Remote patient monitoring (RPM) has shown promise in aiding safe and efficient remote care for chronic conditions; however, its use remains more limited within the hospital at home (HaH) model of care despite a significant opportunity to increase patient eligibility, improve safety, and decrease costs. HaH could achieve these goals by further adopting the 3 primary modalities of RPM (ie, vital sign, continuous single-lead electrocardiogram, and fall monitoring). With only 2 in-person vital sign checks required per day, HaH patient eligibility is currently often limited to lower-acuity cases. The use of vital sign RPM within HaH could better match the standard clinical practice of vital sign checks every 4-8 hours and enable safe care for appropriate moderate-acuity medical and surgical floor-level patients not traditionally enrolled in HaH. Robust, efficient collection of more frequent vital signs via RPM could expand patient eligibility for HaH and create a digital health safety net that enables high quality care. Similarly, our experience at Massachusetts General Hospital has demonstrated that appropriate use of continuous single-lead electrocardiogram RPM can also expand HaH enrollment, particularly for patients with acute decompensated heart failure. Through increasing enrollment of patients in HaH, RPM stands to enable more patients to reap the potential safety benefits of home hospitalization, including decreased rates of delirium and hospital-acquired infections, and better avoid aspects of posthospital syndrome. Furthermore, instituting fall detection RPM allows care teams to further HaH patient safety during their episode of acute care and develop enhanced mitigation strategies to avoid falls post home hospitalization. RPM also has the potential to assist HaH in achieving greater economies of scale and decreasing direct variable costs. By expanding HaH eligibility, RPM could enable HaH programs, which have traditionally operated under capacity, to care for a larger census and decrease allocated fixed costs per hospitalization. Additionally, RPM for HaH could further optimize hybrid in-home and remote nurse or physician evaluations, decreasing costs on a per-episode basis by up to an estimated 3.5%. Overall, RPM holds great promise to increase patient eligibility and patient safety while decreasing costs. However, it is in its infancy in achieving its potential to advance the HaH model of care; further research and experience that inform operational and technical as well as policy considerations are needed.


Asunto(s)
Insuficiencia Cardíaca , Hospitalización , Humanos , Hospitales , Enfermedad Crónica , Monitoreo Fisiológico , Calidad de la Atención de Salud
3.
Aesthet Surg J ; 43(8): 805-816, 2023 07 15.
Artículo en Inglés | MEDLINE | ID: mdl-36967478

RESUMEN

BACKGROUND: Opening the neck through a submental incision allows accurate management of deep neck structures and results in exceptional neck contours. OBJECTIVES: The authors aimed to evaluate the distribution of deep neck structures and investigate the detailed vascular anatomy of the submandibular gland. METHODS: A total of 26 fresh frozen cadaver heads (15 female, 11 male) were utilized. The authors evaluated the weights of the excised tissues simulating cosmetic resections, including subcutaneous fat, subplatysmal fat, the anterior belly of the digastric muscle, and submandibular glands. The vascular supply of the submandibular gland and intracapsular vessel diameters were also investigated. RESULTS: Whereas female cadavers had greater mean tissue weight removed from the supraplatysmal plane (mean 20.9 g, 56.6%) than the subplatysmal plane (16 g, 43.4%), male cadavers had higher mean tissue weight removed from the subplatysmal plane (10.5 g, 60.7%) than the supraplatysmal plane (mean 6.8 g, 39.3%). The mean subcutaneous (6.8 g) and subplatysmal (6.4 g) fat weights were almost equal in male cadavers; mean subcutaneous fat weight (20.9 g) was 3 times higher than subplatysmal fat weight (6.8 g) in female cadavers. There was a statistically significant relationship between body mass index and fat removed. The intraglandular vessel diameters increased as resections approached the main feeding vessels located posterosuperior (facial artery) and anterosuperior (submental artery) to the submandibular gland. CONCLUSIONS: The results suggest that to achieve exceptional neck contour the structures deep to the platysma often need to be addressed. The submandibular gland reduction can be safely performed with comprehensive understanding of its vascular anatomy.


Asunto(s)
Procedimientos de Cirugía Plástica , Glándula Submandibular , Humanos , Masculino , Femenino , Glándula Submandibular/cirugía , Glándula Submandibular/anatomía & histología , Cuello/cirugía , Músculos del Cuello/anatomía & histología , Músculos del Cuello/cirugía , Cadáver
4.
Ann Emerg Med ; 80(5): 401-407, 2022 11.
Artículo en Inglés | MEDLINE | ID: mdl-35940989

RESUMEN

STUDY OBJECTIVE: Emergency clinician-staffed telehealth programs seek to provide equitable, safe, efficient, effective, and patient-centered care. However, early studies show conflicting evidence on whether this aim is accomplished. Furthermore, how programs track the efficacy and safety of their programs remains largely unexplored. We sought to characterize ongoing quality monitoring among emergency clinician-staffed telehealth programs. METHODS: We identified representatives at emergency clinician-staffed telehealth programs through professional networks and published literature. Qualitative interviews were conducted, assessing quality metrics captured as well as motivations for and barriers to quality measurement. We classified quality metric measurement using the National Quality Forum Telehealth Measurement Framework Domains and Subdomains. We developed a codebook from interview transcripts for qualitative analysis to classify motivations for and barriers to quality measurement. RESULTS: We held 8 qualitative interviews with physician representatives at primarily academic (7/8) and urban institutions (5/8). Most widely used quality metrics were related to patient and care team experience (7/8) as well as to access to care (6/8) and effectiveness (6/8). Few programs (2/8) measured finance-related quality metrics. Motivations for quality measurement varied considerably. Common barriers to implementation included technology challenges, data availability, and the lack of quality metric standardization. CONCLUSION: We identified variation in the use and content of quality metrics across emergency clinician-staffed telehealth programs. Most commonly, programs used metrics related to clinical experience; financial metrics were rarely captured. Technology barriers to quality measurement were often cited across programs. Further work is needed to support the standardization and implementation of future quality measurement initiatives.


Asunto(s)
Médicos , Telemedicina , Humanos , Atención Dirigida al Paciente
5.
Am J Emerg Med ; 61: 127-130, 2022 11.
Artículo en Inglés | MEDLINE | ID: mdl-36096014

RESUMEN

OBJECTIVES: Adverse reactions to intravenous (IV) iodinated contrast media are classified by the American College of Radiology (ACR) Manual on Contrast Media as either allergic-like (ALR) or physiologic (PR). Premedication may be beneficial for patients who have prior documented mild or moderate ALR. We sought to perform a retrospective analysis of patients who received computed tomography (CT) imaging in our emergency department (ED) to establish whether listing of an iodinated contrast media allergy results in a delay in care, increases the use of non-contrast studies, and to quantify the incidence of listing iodinated contrast allergies which do not necessitate premedication. METHODS: We performed a retrospective analysis of CT scans performed in our academic medical center ED during a 6-month period. There were 12,737 unique patients of whom 454 patients had a listed iodinated contrast allergy. Of these, 106 received IV contrast and were categorized as to whether premedication was necessary. Descriptive statistics were used to evaluate patient demographics, clinical characteristics, and operational outcomes. A multivariate linear regression model was used to predict time from order to start (OTS time) of CT imaging while controlling for co-variates. RESULTS: Non-allergic patients underwent contrast-enhanced CT imaging at a significantly higher rate than allergic patients (45.9% vs. 23.3%, p < 0.01). The OTS time for allergic patients who underwent contrast-enhanced CT imaging was 360 min and significantly longer than the OTS time for non-allergic patients who underwent contrast-enhanced CT imaging (118 min, p < 0.001). Of the 106 allergic patients who underwent contrast-enhanced CT imaging, 27 (25.5%) did not meet ACR criteria for necessitating premedication. The average OTS time for these 27 patients was 296 min, significantly longer than the OTS for non-allergic patients (118 min, p < 0.01) and did not differ from the OTS time for the 79 patients who did meet premedication criteria (382 min, p = 0.23). A multivariate linear regression showed that OTS time was significantly longer if a contrast allergy was present (p < 0.001). CONCLUSION: A chart-documented iodinated contrast allergy resulted in a significant increase in time to obtain a contrast-enhanced CT study. This delay persisted among patients who did not meet ACR criteria for premedication. Appropriately deferring premedication could potentially reduce the ED length-of-stay by over 4 h for these patients.


Asunto(s)
Medios de Contraste , Hipersensibilidad a las Drogas , Humanos , Medios de Contraste/efectos adversos , Hipersensibilidad a las Drogas/epidemiología , Hipersensibilidad a las Drogas/etiología , Servicio de Urgencia en Hospital , Estudios Retrospectivos , Tomografía Computarizada por Rayos X/métodos
6.
Glob Chang Biol ; 27(23): 6217-6231, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34585498

RESUMEN

Climate warming may be exacerbated if rising temperatures stimulate losses of soil carbon to the atmosphere. The direction and magnitude of this carbon-climate feedback are uncertain, largely due to lack of knowledge of the thermal adaptation of the physiology and composition of soil microbial communities. Here, we applied the macromolecular rate theory (MMRT) to describe the temperature response of the microbial decomposition of soil organic matter (SOM) in a natural long-term warming experiment in a geothermally active area in New Zealand. Our objective was to test whether microbial communities adapt to long-term warming with a shift in their composition and their temperature response that are consistent with evolutionary theory of trade-offs between enzyme structure and function. We characterized the microbial community composition (using metabarcoding) and the temperature response of microbial decomposition of SOM (using MMRT) of soils sampled along transects of increasing distance from a geothermally active zone comprising two biomes (a shrubland and a grassland) and sampled at two depths (0-50 and 50-100 mm), such that ambient soil temperature and soil carbon concentration varied widely and independently. We found that the different environments were hosting microbial communities with distinct compositions, with thermophile and thermotolerant genera increasing in relative abundance with increasing ambient temperature. However, the ambient temperature had no detectable influence on the MMRT parameters or the relative temperature sensitivity of decomposition (Q10 ). MMRT parameters were, however, strongly correlated with soil carbon concentration and carbon:nitrogen ratio. Our findings suggest that, while long-term warming selects for warm-adapted taxa, substrate quality and quantity exert a stronger influence than temperature in selecting for distinct thermal traits. The results have major implications for our understanding of the role of soil microbial processes in the long-term effects of climate warming on soil carbon dynamics and will help increase confidence in carbon-climate feedback projections.


Asunto(s)
Microbiota , Suelo , Carbono , Microbiología del Suelo , Temperatura
7.
Nanotechnology ; 31(24): 245601, 2020 Mar 27.
Artículo en Inglés | MEDLINE | ID: mdl-32249760

RESUMEN

Although homogeneous, high quality graphene can be fabricated on a Cu or Ni sheet using the traditional chemical vapour deposition method at high temperatures (over 1000 °C) under specific atmospheric conditions, their transfer to another substrate is difficult. In this paper a novel method of rapidly (i.e. 3-6 s of laser irradiation) producing a large area (>3 cm2) graphene film from olive oil on a glass surface (pre-coated with a 5-28 nm thick Ni film) with defocused, large area continuous laser irradiation is described. The turbostratic graphene film (6 layers) grown in such a way has shown high electrical conductivity (sheet resistance of around 20 Ω sq-1) and an optical transmittance of 40-50%. With femtosecond laser patterning, 70% optical transparency was demonstrated. Continuous large area graphene was formed at relatively lower temperatures (<250 °C) and without the need for specific atmospheric conditions. The basic process characteristics and mechanisms involved are discussed.

9.
Ann Plast Surg ; 81(3): 353-359, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-29916888

RESUMEN

BACKGROUND: Chest wall recontouring is a common surgical treatment of gender dysphoria in transmen and nonbinary individuals assigned female at birth. This study reviews more than 20 years of cases to identify risk factors for postoperative complications and likelihood of preservation of nipple sensation. METHODS: One hundred thirty-seven cases of female-to-male chest wall recontouring by a single surgeon from 1994 to 2016 were reviewed, 99 of which were included for final analysis. Double-incision free nipple graft and double incision with nipple transposition on a pedicle were the most common techniques used. Complication rates between these 2 techniques were compared, and multivariate analysis was used to identify possible predictors of major complications, and minor complications. RESULTS: No significant risk factors for major complications were identified. With regard to minor complications, advanced patient age (odds ratio [OR], 1.67; P = 0.03) and early surgical experience (OR, 5.08; P = 0.001) were found to be associated with increased risk. Preoperative hormonal treatment was found to trend toward a protective effect (OR, 0.13; P = 0.07). CONCLUSIONS: Any of the reviewed techniques are safe in practice; however, there is a learning curve associated with their use, and longer follow-up will allow for the identification of late complications. The double incision with nipple transposition on a pedicle technique can be considered for patients for whom depigmentation of the nipple-areola complex is a significant concern, especially if they are willing to tolerate a potentially suboptimal chest contour.


Asunto(s)
Disforia de Género/cirugía , Mamoplastia/métodos , Cirugía de Reasignación de Sexo/métodos , Pared Torácica/cirugía , Adolescente , Adulto , Femenino , Estudios de Seguimiento , Humanos , Curva de Aprendizaje , Masculino , Mastectomía , Persona de Mediana Edad , Complicaciones Posoperatorias/epidemiología , Complicaciones Posoperatorias/etiología , Estudios Retrospectivos , Factores de Riesgo , Personas Transgénero , Resultado del Tratamiento , Adulto Joven
10.
Biochem Eng J ; 111: 24-33, 2016 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-27087780

RESUMEN

In vitro-derived platelets (PLTs), which could provide an alternative source of PLTs for patient transfusions, are formed from polyploid megakaryocytes (MKs) that extend long cytoplasmic projections, termed proplatelets (proPLTs). In this study, we compared polyploidization and proPLT formation (PPF) of MKs cultured on surfaces that either promote or inhibit protein adsorption and subsequent cell adhesion. A megakaryoblastic cell line exhibited increased polyploidization and arrested PPF on a low-attachment surface. Primary human MKs also showed low levels of PPF on the same surface, but no difference in ploidy. Importantly, both cell types exhibited accelerated PPF after transfer to a surface that supports attachment, suggesting that pre-culture on a non-adhesive surface may facilitate synchronization of PPF and PLT generation in culture.

12.
Arthroscopy ; 31(4): 707-713.e5, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25543245

RESUMEN

PURPOSE: The purposes of this study were to evaluate patient-reported outcomes after fasciotomy of the leg for chronic exertional compartment syndrome (CECS) and to determine the rate at which revision surgery was required and the prognostic value of intracompartmental pressure (ICP) testing. METHODS: This was a retrospective consecutive case series of patients with CECS who underwent preoperative ICP testing and surgical fasciotomy for treatment of CECS of the leg between September 2001 and January 2012. RESULTS: Of 69 eligible patients, 46 were evaluated at a mean follow-up time of 54.9 months (range, 3.9 to 127.3 months). Forty-two patients met the Pedowitz criteria for CECS diagnosis. Mean score on the Lower Extremity Functional Scale (LEFS) was 70.4 (standard deviation [SD] ± 11.2) at follow-up and 72.3 (SD ± 11.2) at the patient-perceived time of best outcome. Best outcome was reported at a mean time of 14.3 months (range, 0.5 to 84 months). Five of 46 (11%) patients required a revision fasciotomy. Thirty-six of 46 (78%) patients reported being either satisfied (n = 14) or very satisfied (n = 22) at follow-up. The Pedowitz criteria were highly sensitive (97%) but not specific (10%) and had a positive predictive value (PPV) of 79%. CONCLUSIONS: Functional outcomes after fasciotomy for CECS were favorable. ICP testing was shown to be sensitive but not specific. Revision surgery was required for 5 of the 46 patients (11%). Patient satisfaction rates, return to sport, return to preoperative activity levels, and LEFS scores were all high. This case series confirms that fasciotomy is a safe and effective surgical treatment for CECS. LEVEL OF EVIDENCE: Level IV, therapeutic case series.


Asunto(s)
Síndromes Compartimentales/diagnóstico , Síndromes Compartimentales/cirugía , Fasciotomía , Pierna/cirugía , Adolescente , Adulto , Enfermedad Crónica , Femenino , Humanos , Masculino , Manometría , Persona de Mediana Edad , Presión , Pronóstico , Estudios Retrospectivos , Resultado del Tratamiento , Adulto Joven
13.
Tree Physiol ; 2024 Jul 20.
Artículo en Inglés | MEDLINE | ID: mdl-39030692

RESUMEN

We tested an approach to estimate daily canopy net photosynthesis, A, based on estimates of transpiration, E, using measurements of sap flow and water-use efficiency, ω, by measuring δ13C in CO2 respired from shoots in the canopies of two conifers (Podocarpaceae) native to New Zealand. The trees were planted in adjacent 20-year-old stands with the same soil and environmental conditions. Leaf area index was lower for Dacrycarpus dacrydioides (1.34 m2 m-2) than for Podocarpus totara (2.01 m2 m-2) but mean (± standard error) stem diameters were the same at 152 ± 21 mm for D. dacrydioides and 154 ± 25 mm for P. totara. Over a 28-day period, daily A (per unit ground area) ranged almost five-fold but there were no significant differences between species (mean 2.73 ± 1.02 gC m-2 d-1). This was attributable to higher daily values of E (2.63 ± 0.83 mm d-1) and lower ω (1.35 ± 0.53 gC kg H2O-1) for D. dacrydioides compared with lower E (1.82 ± 0.72 mm d-1) and higher ω (1.90 ± 0.77 gC kg H2O-1) for P. totara. We attributed this to higher nitrogen availability and nitrogen concentration per unit foliage area, Na, and greater exposure to irradiance in the D. dacrydioides canopy compared with P. totara. Our findings support earlier observations that D. dacrydioides is more adapted to sites with poor drainage. In contrast, the high retention of leaf area and maintaining low rates of transpiration by P. totara, resulting in higher water-use efficiency, is an adaptive response to survival in dry conditions. Our findings show that physiological adjustments for two species adapted to different environments led to similar canopy photosynthesis rates when the trees were grown in the same conditions. We demonstrated consistency between whole-tree and more intensive shoot-scale measurements, confirming that integrated approaches are appropriate for comparative estimates of carbon uptake in stands with different species.

14.
Acad Radiol ; 31(2): 417-425, 2024 02.
Artículo en Inglés | MEDLINE | ID: mdl-38401987

RESUMEN

RATIONALE AND OBJECTIVES: Innovation is a crucial skill for physicians and researchers, yet traditional medical education does not provide instruction or experience to cultivate an innovative mindset. This study evaluates the effectiveness of a novel course implemented in an academic radiology department training program over a 5-year period designed to educate future radiologists on the fundamentals of medical innovation. MATERIALS AND METHODS: A pre- and post-course survey and examination were administered to residents who participated in the innovation course (MESH Core) from 2018 to 2022. Respondents were first evaluated on their subjective comfort level, understanding, and beliefs on innovation-related topics using a 5-point Likert-scale survey. Respondents were also administered a 21-question multiple-choice exam to test their objective knowledge of innovation-related topics. RESULTS: Thirty-eight residents participated in the survey (response rate 95%). Resident understanding, comfort and belief regarding innovation-related topics improved significantly (P < .0001) on all nine Likert-scale questions after the course. After the course, a significant majority of residents either agreed or strongly agreed that technological innovation should be a core competency for the residency curriculum, and that a workshop to prototype their ideas would be beneficial. Performance on the course exam showed significant improvement (48% vs 86%, P < .0001). The overall course experience was rated 5 out of 5 by all participants. CONCLUSION: MESH Core demonstrates long-term success in educating future radiologists on the basic concepts of medical technological innovation. Years later, residents used the knowledge and experience gained from MESH Core to successfully pursue their own inventions and innovative projects. This innovation model may serve as an approach for other institutions to implement training in this domain.


Asunto(s)
Educación de Postgrado en Medicina , Internado y Residencia , Humanos , Educación de Postgrado en Medicina/métodos , Competencia Clínica , Curriculum , Radiólogos , Hospitales
15.
J Labelled Comp Radiopharm ; 56(2): 36-41, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-24285280

RESUMEN

[carboxyl-(14) C]Celivarone was synthesised from barium [(14) C]carbonate with overall radiochemical yields in the range 49-53%. The synthetic route involves [(14) C]carbonylation methodology, which both decreased the number of synthetic steps and increased the yields obtained from previous synthetic routes.


Asunto(s)
Antiarrítmicos/síntesis química , Benzofuranos/síntesis química , Monóxido de Carbono/síntesis química , Radioisótopos de Carbono/química , Radiofármacos/síntesis química , Bario , Carbonatos/síntesis química , Marcaje Isotópico/métodos
16.
Aging Dis ; 14(3): 621-651, 2023 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-37191415

RESUMEN

Dementia is a progressive neurodegenerative disease leading to deterioration in cognitive and physical skills. Driving is an important instrumental activity of daily living, essential for independence. However, this is a complex skill. A moving vehicle can be a dangerous tool in the hand of someone who cannot maneuver it properly. As a result, the assessment of driving capacity should be part of the management of dementia. Moreover, dementia comprises of different etiologies and stages consisting of different presentations. As a result, this study aims to identify driving behaviors common in dementia and compare different assessment methods. A literature search was conducted using the PRISMA checklist as a framework. A total of forty-four observational studies and four meta-analyses were identified. Study characteristics varied greatly with regards to methodology, population, assessments, and outcome measures used. Drivers with dementia performed generally worse than cognitively normal drivers. Poor speed maintenance, lane maintenance, difficulty managing intersections and poor response to traffic stimuli were the most common behaviors in drivers with dementia. Naturalistic driving, standardized road assessments, neuropsychological tests, participant self-rating and caregiver rating were the most common driving assessment methods used. Naturalistic driving and on-road assessments had the highest predictive accuracy. Results on other forms of assessments varied greatly. Both driving behaviors and assessments were influenced by different stages and etiologies of dementia at varying degrees. Methodology and results in available research are varied and inconsistent. As a result, better quality research is required in this field.

17.
Ann Bot ; 110(2): 253-8, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-22492330

RESUMEN

AIMS AND BACKGROUND: While the temperature response of soil respiration (R(S)) has been well studied, the partitioning of heterotrophic respiration (R(H)) by soil microbes from autotrophic respiration (R(A)) by roots, known to have distinct temperature sensitivities, has been problematic. Further complexity stems from the presence of roots affecting R(H), the rhizosphere priming effect. In this study the short-term temperature responses of R(A) and R(H) in relation to rhizosphere priming are investigated. METHODS: Temperature responses of R(A), R(H) and rhizosphere priming were assessed in microcosms of Poa cita using a natural abundance δ(13)C discrimination approach. RESULTS: The temperature response of R(S) was found to be regulated primarily by R(A), which accounted for 70 % of total soil respiration. Heterotrophic respiration was less sensitive to temperature in the presence of plant roots, resulting in negative priming effects with increasing temperature. CONCLUSIONS: The results emphasize the importance of roots in regulating the temperature response of R(S), and a framework is presented for further investigation into temperature effects on heterotrophic respiration and rhizosphere priming, which could be applied to other soil and vegetation types to improve models of soil carbon turnover.


Asunto(s)
Carbono/metabolismo , Raíces de Plantas/metabolismo , Poa/metabolismo , Microbiología del Suelo , Suelo/química , Respiración de la Célula , Procesos Heterotróficos , Raíces de Plantas/citología , Rizosfera , Temperatura
18.
Lasers Med Sci ; 27(1): 197-204, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21611745

RESUMEN

This paper reports an investigation into the characteristics of femtosecond laser (800-nm central wavelength) in the ablation of human dental enamel, dentine, and cementum at various laser fluences from 0.2 to 3.68 J/cm(2) with single and multiple pulses. The femtosecond laser interaction with cementum is reported for the first time. Ablation thresholds were determined to be 0.58, 0.44, and 0.51 J/cm(2) for enamel, dentine, and cementum, respectively. Under the average laser fluences of 1.13 to 3.68 J/cm(2), clean ablated surfaces without debris and microcracks were obtained. Laser fluence was found to influence the ablated diameter and depth, whereas under a certain fluence, pulse number only affects the depth, without affecting the diameter. The ablation mechanism is found to be based on multi-photon absorption, not previously known for femtosecond laser ablation of dental materials. The low thermal loads of 0.708, 1.44, and 0.404 J/cm(3) required for ablating enamel, dentine, and cementum, determined for the first time, are beneficial for minimizing the heat-affected zones and micro-damage. The Raman spectroscopic analysis of phosphate shows that the chemical components of the tooth remain intact before and after the fs-laser ablation. It also shows that different dental tissues respond differently to the laser irradiation.


Asunto(s)
Cemento Dental/efectos de la radiación , Esmalte Dental/efectos de la radiación , Dentina/efectos de la radiación , Terapia por Láser/instrumentación , Terapia por Láser/métodos , Humanos , Microscopía Electrónica de Rastreo , Espectrometría Raman
19.
Sci Total Environ ; 851(Pt 2): 158274, 2022 Dec 10.
Artículo en Inglés | MEDLINE | ID: mdl-36030860

RESUMEN

The addition of carbon (C) substrate often modifies the rate of soil organic matter (SOM) decomposition. This is known as the priming effect. Nitrous oxide (N2O) emissions from soil are also linked to C substrate dynamics; however, the relationship between the priming effect and N2O emissions from soil is not understood. This study aimed to investigate the effects of C and N substrate addition on the linkages between SOM priming and N2O emissions. We applied 13C-labelled substrates (acetate, butyrate, glucose; 80 µg C g-1), with water as a control, and 15N-labelled N (300 µg N g-1 soil, potassium nitrate) to three different soils, and, after 3 days, we measured the effects on the priming of SOM and sources of N2O emission. Carbon substrate addition increased both CO2- and SOM-derived N2O emissions in the presence of exogenous N. Emissions of CO2 and N2O from soils with added glucose (mean ± standard deviation, 0.73 ± 0.13 µmol m-2 s-1 and 21.4 ± 12.1 mg N m-2 h-1) were higher (p < 0.05) than those from soils treated with acetate (0.64 ± 0.11 µmol m-2 s-1 and 10.9 ± 6.5 mg N m-2 h-1) or butyrate (0.61 ± 0.11 µmol m-2 s-1 and 11.0 ± 6.6 mg N m-2 h-1), respectively. Acetate addition induced a stronger (p < 0.05) priming effect on soil C (0.07 ± 0.09 µmol C m-2 s-1) than that for glucose (0.02 ± 0.10 µmol C m-2 s-1), while butyrate addition resulted in negative priming (-0.09 ± 0.05 µmol C m-2 s-1). SOM-derived N2O emissions were relatively low from soils with butyrate addition (1.4 ± 1.5 mg N m-2 h-1) compared with acetate (2.9 ± 2.3 mg N m-2 h-1) or glucose (9.2 ± 4.5 mg N m-2 h-1). There was no clear relationship between the priming effect and SOM-derived N2O emissions. The observed priming effect related to the potential electron donor supply of the C substrates was not observed. There is a need to further examine the role of soil priming in relation to soil N2O emissions.


Asunto(s)
Óxido Nitroso , Suelo , Óxido Nitroso/análisis , Suelo/química , Carbono , Nitratos , Dióxido de Carbono/análisis , Compuestos Orgánicos , Agua , Glucosa , Butiratos , Agricultura , Fertilizantes
20.
Polymers (Basel) ; 13(13)2021 Jun 29.
Artículo en Inglés | MEDLINE | ID: mdl-34209687

RESUMEN

Carbon fibre reinforced polymer composites (CFRPs) can be costly to manufacture, but they are typically used anywhere a high strength-to-weight ratio and a high steadiness (rigidity) are needed in many industrial applications, particularly in aerospace. Drilling composites with a laser tends to be a feasible method since one of the composite phases is often in the form of a polymer, and polymers in general have a very high absorption coefficient for infrared radiation. The feasibility of sequential laser-mechanical drilling for a thick CFRP is discussed in this article. A 1 kW fibre laser was chosen as a pre-drilling instrument (or initial stage), and mechanical drilling was the final step. The sequential drilling method dropped the overall thrust and torque by an average of 61%, which greatly increased the productivity and reduced the mechanical stress on the cutting tool while also increasing the lifespan of the bit. The sequential drilling (i.e., laser 8 mm and mechanical 8 mm) for both drill bits (i.e., 2- and 3-flute uncoated tungsten carbide) and the laser pre-drilling techniques has demonstrated the highest delamination factor (SFDSR) ratios. A new laser-mechanical sequence drilling technique is thus established, assessed, and tested when thick CFRP composites are drilled.

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