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1.
Cancer Invest ; 42(3): 260-273, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38588003

RESUMEN

In this study, we investigate the veliparib­induced toxicity in cancer patients. Databases were searched for RCTs treated with veliparib. We found veliparib could increase the risk of hematologic and gastrointestinal toxicities. Anemia, neutropenia, thrombocytopenia, and nausea were the most common toxicities. Patients diagnosed with gastrointestinal tumors tend to have a higher risk of high-grade neutropenia; patients in the first-line setting tend to have a higher risk of high-grade anemia and neutropenia than those in the ≥ second line setting. Patients receiving higher dosage of veliparib tend to have a higher risk of all-grade anemia. Veliparib could also increase the risk of insomnia, myalgia, pneumonia, dyspnea, hyponatremia, and fatigue.


Asunto(s)
Bencimidazoles , Neoplasias , Humanos , Bencimidazoles/efectos adversos , Bencimidazoles/uso terapéutico , Neoplasias/tratamiento farmacológico , Inhibidores de Poli(ADP-Ribosa) Polimerasas/uso terapéutico , Inhibidores de Poli(ADP-Ribosa) Polimerasas/efectos adversos , Antineoplásicos/efectos adversos , Anemia/inducido químicamente
2.
Surg Today ; 53(1): 1-11, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-35059844

RESUMEN

The ideal surgical management for reconstruction after excision of congenital biliary dilatation remains controversial. This updated meta-analysis compared the clinical outcomes of hepaticoduodenostomy (HD) and hepaticojejunostomy (HJ) after resection of congenital biliary dilatation. PubMed, Web of Science, Embase, Ovid, and the Cochrane Library were searched for studies published from November 1981 through July 2020. The primary outcomes were the operative time, enteral feeding time, hospital stay, and postoperative complications. The quality and risk of bias were assessed with the Newcastle-Ottawa Quality Assessment Scale. Odds ratios (ORs) and mean differences (MDs) with 95% confidence intervals (CIs) were pooled using random-effects models. Thirteen total studies included 518 (55.76%) HD cases and 411 (44.24%) HJ cases. Five studies were published post-2013; one was a randomized clinical trial. Patients undergoing HD had a shorter hospital stay (MD, 0.40; p = 0.02) and operative time (MD, 59.54; p < 0.00001) and a lower incidence of adhesive intestinal obstruction (OR, 0.20; p = 0.02) than HJ. HD was comparable to conventional HJ with regard to most postoperative outcomes; however, it was associated with a higher incidence of postoperative bilious gastritis (OR, 6.24; p = 0.002). HD is as safe and feasible as HJ with better outcomes in the short run, although reports with long-term follow-up are relatively few. Long-term follow-up will be necessary to monitor possible associated malignancies in the future.


Asunto(s)
Quiste del Colédoco , Humanos , Quiste del Colédoco/cirugía , Vesícula Biliar , Hígado/cirugía , Anastomosis Quirúrgica/efectos adversos , Intestinos/cirugía , Complicaciones Posoperatorias/etiología , Anastomosis en-Y de Roux/efectos adversos , Ensayos Clínicos Controlados Aleatorios como Asunto
3.
Appl Opt ; 61(8): 2079-2088, 2022 Mar 10.
Artículo en Inglés | MEDLINE | ID: mdl-35297899

RESUMEN

We investigate the performance of a dual-hop mixed free space optical (FSO) /radio frequency (RF) system with fixed-gain relaying under direct detection and heterodyne detection techniques. The FSO link is modeled by the Fisher-Snedecor F distribution, which matches well with the experimental data under weak-to-strong turbulence regimes. The RF link experiences κ-µ shadowed fading, which unifies popular RF fading models. The κ-µ shadowed distribution is approximated by an α-µ distribution. Capitalizing on this approximation, closed-form approximate expressions for the cumulative distribution function, the average bit error rate of different modulation schemes, and the ergodic capacity are derived in terms of the bivariate Fox's H function. Moreover, asymptotic analysis is carried out at a high signal-to-noise ratio to further illustrate the obtained diversity order and the influence of system and channel parameters. Numerical results and Monte Carlo simulations are presented to validate the derived approximate expressions.

4.
Pharm Biol ; 60(1): 131-143, 2022 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34978949

RESUMEN

CONTEXT: The bulb of Lilium brownii F. E. Brown (Liliaceae) (LB) is a common Chinese medicine to relieve insomnia. OBJECTIVE: To investigate the molecular mechanism of LB relieving insomnia. MATERIALS AND METHODS: Insomnia model was induced by intraperitoneally injection p-chlorophenylalanine (PCPA) in Wistar rats. Rats were divided into three groups: Control, PCPA (400 mg/kg, i.p. 2 days), LB (598.64 mg/kg, oral 7 days). The levels of 5-hydroxytryptamine (5-HT), norepinephrine (NE), melatonin (MT), and the expression of GABAA, 5-HT1A and MT receptors, as well as pathological changes in hypothalamus, were evaluated. 16S rDNA sequencing and UPLC-MS/MS were used to reveal the change of the intestinal flora and metabolic profile. RESULTS: The adverse changes in the abundance and diversity of intestinal flora and faecal metabolic phenotype altered by PCPA in rats were reversed after LB treatment, accompanied by the up-regulated levels of 5-HT as 8.14 ng/mL, MT as 16.16 pg/mL, 5-HT1A R and GABAA R, down-regulated level of NE as 0.47 ng/mL, and the improvement of pathological phenomena of cells in the hypothalamus. And the arachidonic acid metabolism and tryptophan metabolism pathway most significantly altered by PCPA were markedly regulated by LB. Besides, it was also found that LB reduced the levels of kynurenic acid related to psychiatric disorders and trimethylamine-N-oxide associated with cardiovascular disease. CONCLUSION: The mechanism of LB relieving insomnia involves regulating flora and metabolites to resemble the control group. As a medicinal and edible herb, LB could be considered for development as a health-care food to relieve increasing insomniacs in the future.


Asunto(s)
Medicamentos Herbarios Chinos/farmacología , Lilium/química , Enfermedades Metabólicas/tratamiento farmacológico , Trastornos del Inicio y del Mantenimiento del Sueño/tratamiento farmacológico , Animales , Cromatografía Líquida de Alta Presión , Fenclonina , Microbioma Gastrointestinal/efectos de los fármacos , Ácido Quinurénico/metabolismo , Masculino , Metilaminas/metabolismo , Ratas , Ratas Wistar , Espectrometría de Masas en Tándem
5.
Surg Endosc ; 35(6): 2690-2697, 2021 06.
Artículo en Inglés | MEDLINE | ID: mdl-32556766

RESUMEN

BACKGROUND: Little data are available to assess the learning curve for robot-assisted surgery on choledochal cysts. The aim of this current study is to investigate the characteristics of the learning curve for robot-assisted choledochal cyst excisions using the da Vinci (SI) surgical system in pediatrics. METHODS: A retrospectively collected database comprising all medical records of the first 60 consecutive patients undergoing a robot-assisted choledochal cyst excision and Roux-en-Y hepaticojejunostomy using the da Vinci (SI) surgical system performed by one individual surgeon was studied. Baseline information and postoperative outcomes were collected and then learning curves were analyzed using the cumulative sum (CUSUM) method. Patients were divided into two groups including group A and group B according to the cutoff points of the learning curve. Intraoperative characteristics and short-term outcomes were compared between the two groups. RESULTS: CUSUM plots revealed that the cutoff point of the learning curve was 14 cases. Comparison of the operative time between the two groups revealed that the total operative time (203.71 ± 15.27, 171.28 ± 3.62 min, P < 0.001), docking time (23.79 ± 5.81, 14.50 ± 0.98 min, P < 0.001), and console time (151.86 ± 9.77, 129.15 ± 2.96 min, P < 0.001) were decreased significantly. The intraoperative bleeding (20.36 ± 7.46 vs. 20.43 ± 9.18, P = 0.977), time to taking water (2.89 ± 0.22 vs. 3.04 ± 0.34, P = 0.115), time to starting solids diet (3.73 ± 0.17 vs. 3.79 ± 0.26, P = 0.387), hospital stay (7.51 ± 1.12 vs. 7.54 ± 0.95, P = 0.910), and the postoperative complications did not differ significantly between the two groups. CONCLUSIONS: The learning curve for the robot-assisted choledochal cyst excision and Roux-en-Y hepaticojejunostomy in children is 14 cases. This learning curve can be used as the basis for performance guidance during training in future.


Asunto(s)
Quiste del Colédoco , Laparoscopía , Pediatría , Robótica , Anastomosis en-Y de Roux , Niño , Quiste del Colédoco/cirugía , Humanos , Curva de Aprendizaje , Estudios Retrospectivos , Resultado del Tratamiento
6.
BMC Pediatr ; 21(1): 140, 2021 03 24.
Artículo en Inglés | MEDLINE | ID: mdl-33761926

RESUMEN

BACKGROUND: Using effective scolicidal agents intraoperatively is essential to lessen the recurrence rate of hepatic echinococcosis. However, severe hypernatremia may occur after hypertonic saline (HS) has been applied as the scolicidal agent. The aim of this study is to report on pediatric patients with severe hypernatremia after hepatic echinococcus surgery. METHODS: Patients who presented to West China Hospital between January 2010 and February 2017 were retrospectively analyzed. Children under 16 years with echinococcosis treated by resection were included in the study. RESULTS: A total of 26 children were enrolled in this study, including 16 boys and 10 girls with a median age of 8 (2-16). 24 (92.3 %) cases were cystic echinococcosis (CE) and two (7.7 %) were alveolar echinococcosis (AE). According to Clavien-Dindo classification of surgical complications, the complication rate of all 26 patients was 19.2 %, among which three cases belonged to Grade I, one to Grade III b and 1 to Grade IV. Two children encountered severe hypernatremia (sodium: 155.3 mmol/L and 190.0mmol/L). Data showed classic clinical features of severe hypernatremia: profound and persistent bradycardia, hypotension and coma. After treatment, they recovered well without any neurologic sequelae. All patients were followed up regularly for a median time of 38 months (range 4-89 months); the overall disease-free survival was 100.0 %. CONCLUSIONS: HS irrigation of intra-abdominal echinococcosis may cause acute hypernatremia and severe consequences. Diagnostic suspicion and early intervention are vital tools for avoiding morbidity and mortality.


Asunto(s)
Equinococosis Hepática , Equinococosis , Hipernatremia , Niño , China/epidemiología , Equinococosis Hepática/cirugía , Femenino , Humanos , Hipernatremia/etiología , Masculino , Estudios Retrospectivos
7.
Zhongguo Zhong Yao Za Zhi ; 46(6): 1537-1546, 2021 Mar.
Artículo en Zh | MEDLINE | ID: mdl-33787152

RESUMEN

To systematically evaluate the clinical efficacy and safety of Ginkgo Leaf Tablets(GLT) in the treatment of acute cerebral infarction(ACI). Seven databases both at home and abroad were systematically retrieved from their establishment to March 2020. The data of the included studies were extracted after review and screening. The quality of the included studies was assessed with the Cochrane risk bias assessment tool, and then the included studies were put into Meta-analysis by RevMan 5.3 to evaluate the total cli-nical efficiency, neurological function score, blood lipids and incidence of adverse reactions in treatment of ACI by GLT. Finally, the GRADE system was adopted to evaluate the evidence quality of each outcome indicator and form recommendations. Ten studies involving 886 participants were included, all of which were of low quality. Meta-analysis results showed that,(1)in terms of the total clinical efficiency, GLT+Western medicine was superior to Western medicine alone(RR_(NDS)=1.20, 95%CI[1.06, 1.36], P=0.005; RR_(NIHSS)=1.35, 95%CI[1.09, 1.69], P=0.007), and there was no statistical difference between GLT+Xuesaitong Injection+Wes-tern medicine and Xuesaitong Injection+Western medicine(RR=1.16, 95%CI[1.00, 1.35], P=0.05).(2)In terms of improving neurological function score, GLT+Western medicine was superior to Western medicine alone(MD_(NIHSS[moderate(severe)])=-1.55, 95%CI[-2.22,-0.88], P<0.000 01; MD_(NIHSS(severe))=-7.51, 95%CI[-8.00,-7.02], P<0.000 01; MD_(NDS)=-1.36, 95%CI[-2.39,-0.33], P=0.01), and GLT+Danshen Injection+Western medicine was superior to Danshen Injection+Western medicine(MD_(NDS)=-3.09, 95%CI[-3.84,-2.34], P<0.000 01).(3)In terms of regulating blood lipids, GLT+Western medicine was superior to Wes-tern medicine alone(MD_(TC)=-1.40, 95%CI[-2.13,-0.66], P=0.000 2; MD_(TG)=-1.29, 95%CI[-1.86,-0.73], P<0.000 01; MD_(LDL-C)=-1.48, 95%CI[-2.91,-0.04], P=0.04; MD_(HDL-C)=0.07, 95%CI[0.02, 0.12], P=0.009).(4)In terms of incidence of adverse reactions, there was no statistical difference between GLT+Western medicine and Western medicine alone(RR=0.63, 95%CI[0.30, 1.32], P=0.22). The results of the evaluation showed that the evidence level of each outcome indicator was low, and the recommendation was at weak level. In conclusion, GLT+Western medicine could improve the total clinical efficiency, neurological function score, and blood lipid status, with a low incidence of adverse reactions. However, due to the small amount of included stu-dies, low study quality and low level of evidence, it is expected to carry out clinical studies with standardized design and large sample size in the future to further investigate the clinical efficacy and safety of GLT in the treatment of ACI.


Asunto(s)
Infarto Cerebral , Ginkgo biloba , Infarto Cerebral/tratamiento farmacológico , Humanos , Hojas de la Planta , Comprimidos , Resultado del Tratamiento
8.
Opt Express ; 28(13): 18633-18648, 2020 Jun 22.
Artículo en Inglés | MEDLINE | ID: mdl-32672160

RESUMEN

For an on-orbit lasercom receiver, the space thermal environment, microgravity environment and the adjustment error will deform the optical antenna, distorting the received wavefront and deteriorating the system performance. The axial non-confocal antenna is a deformed antenna caused by the despace of the secondary or primary mirror. Based on the simplified combination method of ray tracing and diffraction theory (simplified CMRD, or SCMRD), the calculation model of the normalized intensity distribution (NID) and the normalized received power (NRP) is established for an axial non-confocal antenna. The numerical results show that, the axial non-confocal antenna will flatten the received light spot, causing interruption of the communication link and deterioration of the bit error rate (BER) performance. The quantitative relations of the NID versus the axial non-confocal distance and the NRP versus the axial non-confocal distance would provide a reference for optical design, structural design, thermal design, assembly and adjustment accuracy design and detector selection. Besides, the numerical results are compared to those calculated by the wavefront fitting method and CMRD. The pros and cons of all methods are discussed. This work would advance the development of extending the SCMRD to analyze other deformed antennas' impact on an on-orbit lasercom receiver.

9.
Pediatr Transplant ; 24(8): e13810, 2020 12.
Artículo en Inglés | MEDLINE | ID: mdl-32845541

RESUMEN

This retrospective study was conducted to examine the development and current status of pediatric liver transplantation (LT) in western China. Clinical, demographic, morbidity, and mortality data were collected to analyze. It included 260 consecutive pediatric LTs performed at three centers in western China between January 2000 and May 2019. Kaplan-Meier graft survival rates at 1, 3, 5, and 10 years were 82.1%, 77.2%, 76.6%, and 76.6%, respectively; corresponding patient survival rates were 84.7%, 80.7%, 80.0%, and 80.0%, respectively. More patients underwent living donor liver transplantation (LDLT; n = 188 (73.4%)) than deceased-donor liver transplantation (DDLT; n = 68 (26.6%)). Survival was better after LDLT (91.5%, 86.6%, and 80.6% at 1, 3, and 5 years, respectively) than after DDLT (80.9%, 72.4%, and 63.9%, respectively; P < .05). Biliary atresia was the leading LT indication (n = 141 (55.1%)), followed by metabolic disease (n = 36 (14.1%)), which was associated with the best recipient survival (88.5% at 5 years). The transplant era and graft-to-recipient body weight ratio (GRWR) also significantly predicted overall survival. Survival rates at 5 years were worst in 2000-2005 (54.5%) and best for GRWRs of 0.8%-4% (80.4%). The development of pediatric LT in western China began slowly, but the quantity and quality of pediatric LT has progressed in recent years. This procedure is now a promising and reliable treatment for children with end-stage liver disease in western China.


Asunto(s)
Trasplante de Hígado/estadística & datos numéricos , Adolescente , Niño , Preescolar , China , Femenino , Supervivencia de Injerto , Humanos , Lactante , Estimación de Kaplan-Meier , Trasplante de Hígado/mortalidad , Masculino , Estudios Retrospectivos
10.
Surg Endosc ; 34(7): 3223-3231, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-32347390

RESUMEN

BACKGROUND: The main treatment of choledochal cysts is the complete resection of the cyst with Roux-en-Y hepaticojejunostomy, which includes open procedures, laparoscopic procedures, and robot-assisted procedures using a da Vinci surgical system. The aim of this current study was to investigate the safety and effectiveness of these three different surgical methods in pediatric choledochal cyst excisions. METHODS: Between January 2015 and December 2018, patients with choledochal cysts treated with open procedures, laparoscopic procedures, or robot-assisted procedures were retrospectively analyzed. The data collected included demographic information of all patients, type and size of cyst, operative details, and postoperative outcomes. RESULTS: A total of 371 episodes of patients were enrolled which consist of the open procedures group (n = 226), laparoscopic procedures group (n = 104), and robot-assisted procedures group (n = 41). The operation time was significantly longer in the laparoscopic procedures group (212.79 ± 34.94) than open procedures group (115.88 ± 13.50) and robot-assisted procedures group (180.61 ± 14.07) (p < 0.001). The volume of intraoperative bleeding were higher in the open procedures group (40.12 ± 55.51) than in the laparoscopic procedures group (21.73 ± 11.44) and robot-assisted procedures group (21.34 ± 9.42), while there was no significant difference between the latter groups. The time to taking water, time to starting liquid diet, and the average length of postoperative hospital stay were similar between the laparoscopic and robot-assisted procedures group, which are shorter than the open procedures group with significant differences. There was no signifcant difference in complications among the three groups. CONCLUSION: Choledochal cyst excision with robotic-assisted procedures had identical surgical effects as open procedures and had lower technical requirements. But it had higher medical cost and better cosmetic effects. Open procedures had largely positive surgical outcomes with fewest complications but poor cosmetic effects. Laparoscopic procedures were the most technique-demanding approaches with positive cosmetic and economic effect. The incidence of complications of laparoscopic procedures decreased with the learning curve.


Asunto(s)
Anastomosis en-Y de Roux/métodos , Quiste del Colédoco/cirugía , Yeyunostomía/métodos , Laparoscopía/métodos , Procedimientos Quirúrgicos Robotizados/métodos , Niño , Preescolar , Conducto Colédoco/cirugía , Femenino , Humanos , Lactante , Yeyuno/cirugía , Curva de Aprendizaje , Tiempo de Internación/estadística & datos numéricos , Hígado/cirugía , Masculino , Tempo Operativo , Estudios Retrospectivos , Resultado del Tratamiento
12.
Eur J Pediatr ; 178(10): 1537-1544, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31446463

RESUMEN

We performed a prospective study to explore a diagnosis and treatment protocol of transient intussusception in children (TIC). Totally, 143 children with intussusception who met the inclusion criteria were firstly divided into intussusception involving only the small bowel and intussusception involving the colon group. And in each group, they were further divided into short-segment (≤ 3.0 cm) and long-segment (> 3.0 cm) groups according to the length of intussusception. After a period of conservative treatment, the incidence of TIC, the incidence of surgery, and recurrence were collected and analyzed. Finally, we found that the incidence of TIC in the short-segment group of small bowel intussusception (96.29%) was significantly higher than that in other groups (P ≤ 0.001). Besides, the incidence of surgery and recurrence in this group was relatively low too. Therefore, we summarized the inclusion criteria and treatments to the short-segment group of small bowel intussusception as the suggested protocol to TIC.Conclusion: For cases of small bowel intussusception with no identified pathologic lead point, a short duration of symptoms, a length of ≤ 3.0 cm, a relatively abundant vascular flow signal, and a stable general condition, the spontaneous reduction could be expected and a period of conservative treatment with careful monitoring is recommended. What is Known: • The phenomenon of spontaneous reduction in intussusception (transient intussusception) among pediatric patients has been widely reported. • To distinguish the transient intussusception from the other types is important for the transient ones only need conservative treatment rather than enema reduction or surgery. What is New: • This is the first prospective study to explore a diagnosis and treatment protocol of transient intussusception in children. • Short-segment small bowel intussusceptions have a higher rate (96.29%) to get spontaneous reduction than the other types of intussusception.


Asunto(s)
Enfermedades del Colon/terapia , Tratamiento Conservador/métodos , Intestino Delgado , Intususcepción/terapia , Preescolar , Enema/efectos adversos , Femenino , Humanos , Lactante , Intususcepción/diagnóstico por imagen , Masculino , Estudios Prospectivos , Remisión Espontánea , Ultrasonografía
13.
Nanotechnology ; 29(22): 225703, 2018 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-29451131

RESUMEN

Radiation effects on semiconductor nanowires (NWs) have attracted the attention of the research community due to their potential applications in space and atomic fields. The effective implementation of NW devices in a radiation environment is a matter of concern. Here, the photoluminescence (PL) and time-resolved PL (TRPL) measurements were performed on both GaAs and InP NWs at room temperature before and after 1 MeV H+ irradiation with fluences ranging from 1 × 1011 to 5 × 1013 p cm-2. It is found that the degradation of lifetime is size-dependent, and typically the minority carrier lifetime damage coefficient is closely correlated with the material and NW diameter. Compared to GaAs and InP bulk material counterparts, the lifetime damage coefficient of NWs decreases by a factor of about one order of magnitude. After irradiation, GaAs NWs with a smaller diameter show a much lower lifetime damage coefficient while InP NWs show an increase in carrier radiative lifetime. The increased size-dependent radiation hardness is mainly attributed to the defect sink effect and/or the improvement of a room temperature dynamic annealing mechanism of the NWs. The InP NWs also showed higher radiation tolerance than GaAs NWs.

14.
BMC Pediatr ; 18(1): 320, 2018 10 09.
Artículo en Inglés | MEDLINE | ID: mdl-30301467

RESUMEN

BACKGROUND: Colonic stenosis is a rare cause of pediatric intestinal obstruction. The root cause underlying colonic stenosis is unclear and there is no fixed operation. CASE PRESENTATION: We reported on a male infant with progressive colonic stenosis caused by antibiotic-related colitis. The infant was admitted to our hospital with pneumonia but developed progressive abdominal distension and diarrhea following antibiotic treatment with meropenem. Initial testing of stool culture showed a Clostridium difficile infection. Additional testing with barium enema imaging showed stenosis at the junction of the sigmoid and descending colon at first and another stenosis occurred at the right half of the transverse colon 3 weeks later. Staged surgical treatment was performed with primary resections of the two parts suffering stenosis, ileostomy, and secondary intestinal anastomosis. A pathological exam then confirmed the diagnosis of colonic stenosis and the patient had an uneventful recovery and has been recovering well as evidenced by the 1-year follow-up. CONCLUSIONS: Based on a review of the literature and our case report, we found that progressive colonic stenosis caused by colitis due to antibiotic-related Clostridium difficile infection is rare in infants. Infants with colitis and repeated abdominal distention, vomiting, and constipation should be treated with the utmost caution and screened. Despite this, clinical manifestations depended on the severity of the stenosis. Barium enema, colonoscopy, laprascopy or laparotomy and colonic biopsy are helpful for diagnosis and differential diagnosis. While both one-stage and multiple-stage operations are feasible, a staged operation should be used for multiple colonic stenoses.


Asunto(s)
Antibacterianos/efectos adversos , Infecciones por Clostridium/tratamiento farmacológico , Enfermedades del Colon/etiología , Enfermedades del Colon/patología , Obstrucción Intestinal/etiología , Meropenem/efectos adversos , Antibacterianos/uso terapéutico , Infecciones por Clostridium/complicaciones , Enfermedades del Colon/diagnóstico por imagen , Enfermedades del Colon/cirugía , Constricción Patológica/diagnóstico por imagen , Constricción Patológica/cirugía , Humanos , Recién Nacido , Obstrucción Intestinal/diagnóstico por imagen , Obstrucción Intestinal/cirugía , Masculino , Meropenem/uso terapéutico , Radiografía
15.
Genet Mol Biol ; 37(3): 540-8, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25249777

RESUMEN

To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, HB = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient of gene differentiation (Gst = 0.2742) and Bayesian analysis (θ B = 0.1963). The Mantel test revealed no significant correlation between genetic and geographic distances among populations (r = 0.1176, p = 0.3686). UPGMA showed that the five cultivated populations were separated into three clusters, which was in good accordance with the results provided by the Bayesian software STRUCTURE (K = 3). A short domestication history and no artificial selection may be an effective way of maintaining and conserving the gene pools of wild R. tanguticum.

16.
Int J Endocrinol ; 2024: 7330133, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39345904

RESUMEN

Objective: The metabolic effects of metabolic syndrome (MetS) on musculoskeletal metabolism are controversial. This study explored the effect of MetS on bone mineral density (BMD) and muscle quality index (MQI). Methods: Data from the NHANES database from 2011 to 2014 were extracted, and nonpregnant participants aged 45-59 years were included. The included data were first weighted by complex sampling, and then, the effect of MetS on BMD and MQI was analyzed using multifactorial linear regression. We then performed a stratified analysis by gender and BMI classification. Moreover, a mediation analysis of MetS on BMD was conducted, with MQI as a mediating variable. A propensity score matching analysis method with a complex sampling design was additionally performed to verify the stability of the results. Results: A total of 1943 participants were eventually included. After adjusting for covariates, the results of linear regression show that MetS is associated with elevated pelvic BMD (beta = 0.03; 95% CI = 0.01, 0.06; P=0.02) and reduced MQI, especially arm MQI (beta = -1.02; 95% CI = -1.27, -0.77; P < 0.0001). MetS is more associated with BMD in women, MQI in normal or heavyweight, and BMD in lightweight, according to stratified analysis. MQI explains the indirect effect of MetS on BMD (beta = 0.007; 95% CI = 0.003, 0.010). Conclusion: This study provides evidence that MetS elevates BMD and reduces MQI, and further, that there is a mediating effect of MQI on elevated BMD.

17.
J Vis Exp ; (209)2024 Jul 26.
Artículo en Inglés | MEDLINE | ID: mdl-39141564

RESUMEN

The study of oocyte development holds significant implications in developmental biology. The zebrafish (Danio rerio) has been extensively used as a model organism to investigate early developmental processes from oocyte to embryo. In zebrafish, oocytes are surrounded by a single layer of somatic granulosa cells. However, separating granulosa cells from oocytes poses a challenge, as achieving pure oocytes is crucial for precise analysis. Although various methods have been proposed to isolate zebrafish oocytes at different developmental stages, current techniques fall short in removing granulosa cells completely, limiting the accuracy of genome analysis focused solely on oocytes. In this study, we successfully developed a rapid and efficient process for isolating pure stage I oocytes in zebrafish while eliminating granulosa cell contamination. This technique facilitates biochemical and molecular analysis, particularly in exploring epigenetic and genome structure aspects specific to oocytes. Notably, the method is user-friendly, minimizes oocyte damage, and provides a practical solution for subsequent research and analysis.


Asunto(s)
Células de la Granulosa , Oocitos , Pez Cebra , Animales , Oocitos/citología , Femenino , Células de la Granulosa/citología
18.
Arthritis Res Ther ; 26(1): 5, 2024 01 02.
Artículo en Inglés | MEDLINE | ID: mdl-38167504

RESUMEN

BACKGROUND: There is mounting proof that rheumatoid arthritis (RA) and cognitive decline are related. These studies, however, have not all been uniform, and others have not discovered such a correlation. It is essential to investigate the link between RA and cognitive decline. METHOD: We conducted a Mendelian randomization analysis utilizing three different publicly accessible RA GWAS summary datasets and a variety of meticulously verified instrumental variables. We mostly used inverse variance weighting (IVW), as well as MR-Egger, weighted median, MR-PRESSO, and several sensitivity analyses, to figure out the link between RA and cognitive impairment (CI). RESULTS: Our MR study identified the causality between RA and declining cognitive performance (ß = - 0.010, 95% CI of - 0.017 to - 0.003, P = 4.33E-03) and cognitive function (ß = - 0.029, 95% CI of - 0.053 to - 0.005, P = 1.93E-02). The consistent direction of the connection is revealed by sensitivity analysis utilizing the weighted median and the MR-Egger method. Furthermore, we reproduced our findings across two additional RA datasets and found identical outcomes, strengthening the validity of our findings. CONCLUSION: This study offers proof of causality between RA and an increased risk of CI. Our findings highlight the importance of examining RA patients for cognitive ability, which may open up fresh ideas for the prevention of CI.


Asunto(s)
Artritis Reumatoide , Disfunción Cognitiva , Humanos , Análisis de la Aleatorización Mendeliana , Disfunción Cognitiva/genética , Cognición , Artritis Reumatoide/genética , Estudio de Asociación del Genoma Completo
19.
Materials (Basel) ; 17(18)2024 Sep 10.
Artículo en Inglés | MEDLINE | ID: mdl-39336192

RESUMEN

During the hot dip aluminum plating process, components such as sinking rollers, pulling rollers, and guide plates will come into long-term contact with high-temperature liquid aluminum and be corroded by the aluminum liquid, greatly reducing their service life. Therefore, the development of a material with excellent corrosion resistance to molten aluminum is used to prepare parts for the dipping and plating equipment and protect the equipment from erosion, which can effectively improve the production efficiency of the factory and strengthen the quality of aluminum-plated materials, which is of great significance for the growth of corporate profits. With AlFeNiCoCr as the binder phase and ZrB2 as the hard phase, ZrB2-based ceramic composites were prepared by spark plasma sintering (SPS). SEM, EDS and XRD were used to characterize the microstructure and properties of the sintered, corroded, and abraded material samples. The density, fracture toughness, corrosion rate and wear amount of the composite material were measured. The results show that ZrB2-AlFeNiCoCr ceramics have compact structure and excellent mechanical properties, and the density, hardness and fracture toughness of ZrB2-AlFeNiCoCr increase with the increase in sintering temperature. However, when the composite material is at 1600 °C, the relative density of the sintering at 1600 °C decreases due to the overflow of the bonding phase. Therefore, when the sintering temperature is 1500 °C, the high entropy alloy has the best performance. The average corrosion rate of ZrB2-1500 at 700 °C liquid aluminum is 1.225 × 10-3 mm/h, and the wear amount in the friction and wear test is 0.104 mm3.

20.
J Ethnopharmacol ; 325: 117641, 2024 May 10.
Artículo en Inglés | MEDLINE | ID: mdl-38151179

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Abelmoschus manihot (L.) Medik. Seeds (AMS, སོ་མ་ར་ཛ།), a Tibetan classical herbal in China, are rich in flavonoids and phenolic glycosides compounds, such as quercetin and its derivatives. Moreover, it has been found to possess anti-rheumatoid arthritis (RA) effects. Nonetheless, its anti-RA mechanism is yet unknown. AIM OF THE STUDY: This research aimed to examine the active ingredients of AMS as well as potential pharmacological mechanisms in AMS on RA. MATERIALS AND METHODS: The ultra-performance liquid chromatography-electrospray ionization-tandem multistage mass spectrometry (UPLC-ESI-IT-MSn) technique was used to determine the primary chemical components of AMS that were responsible for the therapeutic effects on RA. In addition, 36 male Wistar rats weighing between 200 and 220 g were classified at random into six groups [normal control group, collagen-induced arthritis (CIA) group, methotrexate group (positive control, 1.05 mg/kg), AMS group (157.5 mg/kg, 315 mg/kg, 630 mg/kg)]. CIA rats were given AMS extract by intragastric administration for 28 days, and their ankles were photographed to observe the degree of swelling. Further, the arthritis score, paws swelling, and body weight changes of CIA rats were determined to observe whether AMS has any effect on RA, and synovial and cartilage tissue injuries were identified by histopathology. Besides, the levels of IL-10, TNF-α, IL-1ß, INF-γ, etc. in serum were estimated by ELISA. Western blot experiments were implemented to identify the expression levels of protein involved in the JAK2/STAT3 signaling pathway in the CIA rats' synovial tissues. Moreover, the mechanisms and targets of active ingredient therapy of AMS for RA were predicted using network pharmacology and then verified using molecular docking. RESULT: In the present study, 12 compounds were detected by UPLC-ESI-IT-MSn, such as quercetin and its derivative which could be potential active ingredients that contribute to the anti-RA properties of AMS. Our in vivo studies on CIA rats revealed that an AMS-H dose of 630 mg/kg significantly improved joint damage while decreasing the arthritic index and paw swelling. Furthermore, AMS inhibited the INF-γ, IL-6, IL-17, IL-1ß, and TNF-α, levels while upregulating the expression of anti-inflammatory cytokines IL-10 and IL-4 in serum. Besides, AMS inhibited the protein Bcl-2/Bax, STAT3, and JAK2 levels, and promoted the expression of Caspase3, SOCS1, and SOCS3 in the JAK2/STAT3 pathway. Additionally, the JAK/STAT signaling pathway was found to perform a remarkable function in the AMS therapy of RA as evidenced by enrichment in GO terms and KEGG pathways. Meanwhile, data from molecular docking experiments indicated that the core targets of PIK3CA, JAK2, and SRC bound stably to the active ingredients of mimuone, 4'-methoxy-bavachromanol, and quercetin. CONCLUSION: According to these findings, the AMS could improve joint inflammation in CIA rats, and its underlying mechanism could be linked to the regulation of the JAK2/STAT3 pathway. Therefore, AMS might become a promising agent for alleviating inflammation in RA patients.


Asunto(s)
Abelmoschus , Artritis Experimental , Artritis Reumatoide , Humanos , Ratas , Masculino , Animales , Interleucina-10/metabolismo , Ratas Wistar , Factor de Necrosis Tumoral alfa/metabolismo , Simulación del Acoplamiento Molecular , Quercetina/farmacología , Artritis Reumatoide/tratamiento farmacológico , Transducción de Señal , Inflamación/tratamiento farmacológico , Artritis Experimental/patología , Semillas/metabolismo , Janus Quinasa 2/metabolismo , Factor de Transcripción STAT3/metabolismo
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