RESUMEN
In this study, a novel polyurethane membrane, modified by superfine silk-fibroin powder, was prepared for small-diameter vascular grafting. Scanning electron microscopy, transmission electron microscopy, and histological examination were applied to evaluate histocompatibility of this polyurethane membrane. The polyurethane membrane was compared with polytetrafluoroethylene material. A pseudomembrane and gap formed between polytetrafluoroethylene and the surrounding tissues, and no cells infiltrated or grew into the polytetrafluoroethylene material. On the contrary, superfine silk-fibroin powder/polyurethane blend membrane merged tightly with the surrounding tissues without gaps, and cells infiltrated and grew into the material. Moreover, the negative effects of superfine silk-fibroin powder/polyurethane blend membrane on cells were less than those of its polytetrafluoroethylene counterpart. Our findings indicated that the superfine silk-fibroin powder/polyurethane blend membrane has better histocompatibility than polytetrafluoroethylene membrane. It is concluded that the superfine silk-fibroin powder/polyurethane blend membrane is a promising biomaterial for small-diameter prosthesis.
Asunto(s)
Materiales Biocompatibles , Fibroínas/química , Implantes Experimentales , Poliuretanos/química , Animales , Membranas Artificiales , Ratones , Músculo Esquelético/cirugía , Polvos , Distribución Aleatoria , Injerto VascularRESUMEN
To find out which biomaterial had the best biocompatibility, we compared the acute phase reaction of common biomaterials preparing for vascular grafts with the material of polyurethane modified by silk fibroin (SF-PU(1:1)). After transplanted the materials of dacron, polyterafluoroethylene (e-PTFE), polyurethane (PU), SF-PU(1:1) in rat muscle for one week, we studied the influence of different biomaterials on the histocompatibility by using rat acute toxicity test, test of local reaction in muscle, tissue section staining, WBC and PLT count. As a result, dacron had the worst histocompatibility. The other biomaterials had slight local inflammatory reaction. The WBC and PLT was nearly the same with the blank except dacron. e-PTFE, pure PU and SF-PU(1:1) had the better histocompatibility than traditional dacron. Especially SF-PU(1:1) had the best histocompatibility. Because of the better physical properties and histocompatibility of SF-PU( 1:1), the prospect of preparing small-diameter vascular grafts with SF-PU was cheerful.
Asunto(s)
Implantación de Prótesis Vascular/instrumentación , Prótesis Vascular , Fibroínas/química , Ensayo de Materiales/métodos , Poliuretanos/química , Reacción de Fase Aguda , Animales , Materiales Biocompatibles , Femenino , Implantes Experimentales , Sustancias Macromoleculares/química , Masculino , Músculo Esquelético/fisiología , Diseño de Prótesis , Distribución Aleatoria , Ratas , Ratas Sprague-DawleyAsunto(s)
Anticoagulantes/química , Portadores de Fármacos , Fibroínas/química , Heparina/química , Poliuretanos/química , Anticoagulantes/farmacología , Coagulación Sanguínea/efectos de los fármacos , Química Farmacéutica , Preparaciones de Acción Retardada , Composición de Medicamentos , Heparina/farmacología , Humanos , Cinética , Membranas Artificiales , Tiempo de Tromboplastina Parcial , Polvos , Tiempo de Protrombina , Solubilidad , Tecnología Farmacéutica/métodos , Tiempo de TrombinaRESUMEN
ibility than polytetrafluoroethylene mem-brane. It is concluded that the superfine silk-fibroin powder/polyurethane blend membrane is a prom-ising biomaterial for small-diameter prosthesis.