RESUMEN
BACKGROUND: Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy. METHODS: Natural Pru p 7 (nPru p 7) was purified from peach crude extract using a monoclonal antibody affinity column. Complementary DNA for Pru p 7 was cloned and expressed in Escherichia coli and Pichia pastoris. Serum immunoglobulin (Ig) E in peach-allergic patients was examined by enzyme-linked immunosorbent assay (ELISA) using nPru p 7 and rPru p 7 (E. coli product: erPru p 7 and P. pastoris product: prPru p 7). RESULTS: Peach-allergic patients (n=27) were diagnosed and categorized into oral reaction (n=10) or systemic reaction (n=17). The nPru p 7 positivity based on serum IgE levels was 52% in the systemic-reaction group and 0% in the oral-reaction group (P<0.05). In the systemic-reaction group, there was no significant difference in reactivity between nPru p 7 and prPru p 7, but the reactivity of erPru p 7 was significantly lower than those of nPru p 7 and prPru p 7 (P<0.05). CONCLUSIONS: We found that prPru p 7 exhibited reactivity in ELISA comparable to that of nPru p 7 for the diagnosis of peach allergy with systemic reaction.
Asunto(s)
Antígenos de Plantas/inmunología , Hipersensibilidad a los Alimentos/diagnóstico , Inmunoglobulina E/sangre , Prunus persica/efectos adversos , Adolescente , Adulto , Antígenos de Plantas/administración & dosificación , Antígenos de Plantas/efectos adversos , Proteínas Portadoras/administración & dosificación , Proteínas Portadoras/efectos adversos , Proteínas Portadoras/inmunología , Niño , Ensayo de Inmunoadsorción Enzimática , Femenino , Hipersensibilidad a los Alimentos/sangre , Hipersensibilidad a los Alimentos/inmunología , Giberelinas/administración & dosificación , Giberelinas/efectos adversos , Giberelinas/inmunología , Humanos , Masculino , Persona de Mediana Edad , Proteínas Recombinantes/administración & dosificación , Proteínas Recombinantes/efectos adversos , Proteínas Recombinantes/inmunología , Adulto JovenRESUMEN
Here we report a rare case of neutrophilic dermatoses related to a granulocyte colony-stimulating factor (G-CSF)-producing solid pseudopapillary tumour (SPT). The patient was a 39-year-old woman presenting with scattered pustules and crusts of the palms, heels and thighs and plaques of the bilateral lower legs. The skin biopsy revealed dense neutrophil infiltration in the epidermis to the dermis. A pancreatic head tumour was detected using computed tomography. A pathological examination of the resected specimen suggested an SPT. As the skin eruption promptly disappeared after SPT resection, we hypothesized that SPT secretes growth factors including epidermal growth factor (EGF) and G-CSF. The SPT cells stained positive for both EGF and G-CSF tumour cells. The serum levels of interleukin (IL)-6 and IL-10 and tumour necrosis factor-α were within normal limits before and after the SPT resection. In contrast, the serum IL-8, EGF and G-CSF levels decreased after the SPT resection. This is a rare case of neutrophilic dermatoses related to a G-CSF-producing SPT. The present case suggests that physicians should be aware that a G-CSF-producing tumour is a differential diagnosis to consider in patients with unusual aseptic pustulosis.
Asunto(s)
Carcinoma Papilar/complicaciones , Factor Estimulante de Colonias de Granulocitos/biosíntesis , Neoplasias Pancreáticas/complicaciones , Enfermedades de la Piel/etiología , Adulto , Femenino , Humanos , PiernaRESUMEN
Fexofenadine, a histamine H1-receptor antagonist, is approved for the treatment of pruritus associated with atopic dermatitis. The effects of fexofenadine on scratching behaviour, and plasma levels of histamine and eotaxin were assessed in a new model of atopic dermatitis. Mice fed a diet low in Mg2+ and Zn2+ (special diet S) were compared with mice on a normal diet (N) or diet S plus fexofenadine HCl for weeks 0-10 (S + F(0-10)), 0-5 (S + F(0-5)) or 6 - 10 (S + F(6-10)) (seven mice per group). Compared with group N, group S mice showed significantly greater scratching frequency, and plasma histamine and eotaxin concentrations; these three variables were significantly lower in group S + F(0-10) than in group S. Scratching frequency increased when fexofenadine was discontinued. Fexofenadine significantly reduced mast cell and eosinophil numbers. Histamine may be important in the pathological changes seen in this model of atopic dermatitis, suggesting that it might aid future development of antihistamines for the treatment of atopic dermatitis.
Asunto(s)
Dermatitis Atópica/tratamiento farmacológico , Prurito/tratamiento farmacológico , Terfenadina/análogos & derivados , Animales , Recuento de Células , Quimiocina CCL11 , Quimiocinas CC/sangre , Dermatitis Atópica/sangre , Dermatitis Atópica/patología , Dieta , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Eosinófilos/efectos de los fármacos , Eosinófilos/patología , Histamina/sangre , Magnesio/administración & dosificación , Mastocitos/efectos de los fármacos , Mastocitos/patología , Ratones , Prurito/sangre , Prurito/patología , Terfenadina/farmacología , Zinc/administración & dosificaciónAsunto(s)
Anafilaxia/inducido químicamente , Proteínas Portadoras/efectos adversos , Hipersensibilidad a los Alimentos/etiología , Helianthus/inmunología , Semillas/inmunología , Anafilaxia/diagnóstico , Anafilaxia/etiología , Proteínas Portadoras/análisis , Femenino , Humanos , Inmunoglobulina E/sangre , Extractos Vegetales/análisis , Proteínas de Plantas/análisis , Adulto JovenRESUMEN
We aimed to develop an animal model for atopic dermatitis. HR-1 hairless mice fed a diet with reduced magnesium and zinc levels were compared with mice fed a standard diet. Skin dryness and wrinkle-like changes, scratching behaviour, decreased skin water content, increased transepidermal water loss and raised blood immunoglobulin E levels were seen in the group receiving the reduced magnesium and zinc diet compared with control mice. There were no significant differences in body weight or the weight of the major organs between the two groups. Haematological examination in both groups was normal apart from increased immunoglobulin E levels in mice fed a reduced magnesium and zinc diet. These mice may be useful models of atopic dermatitis; preparation of the animals is not particularly time consuming, the reproducibility is 100%, and atopic dermatitis symptoms occur even in a specific pathogen-free environment.
Asunto(s)
Dermatitis Atópica/patología , Dieta , Modelos Animales de Enfermedad , Magnesio/metabolismo , Zinc/metabolismo , Alimentación Animal , Animales , Peso Corporal , Dermatitis Atópica/etiología , Inmunoglobulina E/sangre , Masculino , Ratones , Tamaño de los Órganos , Piel/patología , Factores de TiempoAsunto(s)
Antiinfecciosos/efectos adversos , Dermatitis Alérgica por Contacto/etiología , Dermatitis Profesional/etiología , Piridinas/efectos adversos , Sulfonas/efectos adversos , Adulto , Anciano , Antiinfecciosos/análisis , Dermatitis Alérgica por Contacto/diagnóstico , Dermatitis Profesional/diagnóstico , Cromatografía de Gases y Espectrometría de Masas , Humanos , Diseño Interior y Mobiliario , Masculino , Piridinas/análisis , Sulfonas/análisisRESUMEN
BACKGROUND: Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy. METHODS: Natural Pru p 7 (nPru p 7) was purified from peach crude extract using a monoclonal antibody affinity column. Complementary DNA for Pru p 7 was cloned and expressed in Escherichia coli and Pichia pastoris. Serum immunoglobulin (Ig) E in peach-allergic patients was examined by enzyme-linked immunosorbent assay (ELISA) using nPru p 7 and rPru p 7 (E. coli product: erPru p 7 and P. pastoris product: prPru p 7). RESULTS: Peach-allergic patients (n = 27) were diagnosed and categorized into oral reaction (n=10) or systemic reaction (n = 17). The nPru p 7 positivity based on serum IgE levels was 52% in the systemic-reaction group and 0% in the oral-reaction group (P<0.05). In the systemic-reaction group, there was no significant difference in reactivity between nPru p 7 and prPru p 7, but the reactivity of erPru p 7 was significantly lower than those of nPru p 7 and prPru p 7 (P < 0.05). CONCLUSIONS: We found that prPru p 7 exhibited reactivity in ELISA comparable to that of nPru p 7 for the diagnosis of peach allergy with systemic reaction
No disponible