Few-Body Perspective of a Quantum Anomaly in Two-Dimensional Fermi Gases.

A quantum anomaly manifests itself in the deviation of the breathing mode frequency from the scale invariant value of 2ω in two-dimensional harmonically trapped Fermi gases, where ω is the trapping frequency. Its recent experimental observation with cold atoms reveals an unexpected role played by the effective range of interactions, which requires a quantitative theoretical understanding. Here we provide accurate, benchmark results on a quantum anomaly from a few-body perspective. We consider the breathing mode of a few trapped interacting fermions in two dimensions up to six particles and present the mode frequency as a function of scattering length for a wide range of effective range. We show that the maximum quantum anomaly gradually reduces as the effective range increases while the maximum position shifts towards the weak-coupling limit. We extrapolate our few-body results to the many-body limit and find a good agreement with the experimental measurements. Our results may also be directly applicable to a few-fermion system prepared in microtraps and optical tweezers.

Cluster Formation in Two-Component Fermi Gases.

Two-component fermions are known to behave like a gas of molecules in the limit of Bose-Einstein condensation of diatomic pairs tightly bound with zero-range interactions. We discover that the formation of cluster states occurs when the effective range of two-body interaction exceeds roughly 0.46 times the scattering length, regardless of the details of the short-range interaction. Using an explicitly correlated Gaussian basis set expansion approach, we calculate the binding energy of cluster states in trapped few-body systems and show the difference of structural properties between cluster states and gaslike states. We identify the condition for cluster formation and discuss the potential observation of cluster states in experiments.

[Aspirin inhibits cell stemness of esophageal cancer by downregulation of chemokine CCL2].

Objective: To investigate the effect of tumor-associated macrophages on the stemness of esophageal cancer cells and the potential mechanism of antiproliferative effects of aspirin (ASA). Methods: The effects of aspirin on the stemness characteristics of KYSE-450 cells and KYSE-450 cells co-cultured with M2 macrophages (KYSE-450+ M2) were performed using spheroid formation assay. After treatment with aspirin, the expression of different chemokines, the core pluripotency gene Nanog and the stem cell marker CD90 in different cell groups were determined by real-time quantitative PCR, flow cytometry and Western blot. Results: The number of spheres formed in the ASA and KYSE-450+ M2 cell groups were 7.00±1.23 and 34.33±2.33, respectively, showing statistically significant difference compared with that of control group (14.50±2.33, all P<0.05). The number of spheres in KYSE-450+ M2+ ASA cell group were 20.67±2.33, which was significantly lower than that of KYSE-450+ M2 group (P<0.05). The expression levels of Nanog gene in control and ASA groups were 1.00 and 0.50±0.10, respectively, and the difference was statistically significant (P<0.05). Moreover, the expression of Nanog gene in cells of KYSE-450+ M2 group and M2+ KYSE-450+ ASA group was 1.74±0.13 and 1.43±0.05, showing statistically significant difference (P<0.05). When chemokine CCL2 was knocked down, the levels of Nanog gene in M2+ shCCL2-KYSE450+ ASA group and M2+ shCCL2-KYSE450 group were decreased to 1.22±0.11 and 1.17±0.08, respectively, and there was no statistically significant difference between them (P=0.69). Flow cytometry analyses showed that the expression levels of CD90 in control and ASA cells were (2.93±0.52)% and (1.30±0.17)%, respectively, and the difference was statistically significant (P<0.05). Moreover, the expression levels of CD90 in M2+ shCCL2-KYSE450 cells and M2+ shCCL2-KYSE450+ ASA cells were (4.07±0.12)% and (4.73±0.38)%, respectively, showing no statistically significant difference (P=0.17). Conclusions: Tumor-associated macrophages enhances the stemness of esophageal cancer cells, whereas aspirin attenuates the stemness by suppressing the expression of CCL2. Aspirin plays an anti-tumor effect in esophageal cancer cells.

Multiple variants in 5q31.1 are associated with systemic lupus erythematosus susceptibility and subphenotypes in the Han Chinese population.

BACKGROUND: A previous study provided evidence for a genetic association between PPP2CA on 5q31.1 and systemic lupus erythematosus (SLE) across multi-ancestral cohorts, but failed to find significant evidence for an association in the Han Chinese population. OBJECTIVES: To explore the association between this locus and SLE using data from our previously published genome-wide association study (GWAS). METHODS: Single-nucleotide polymorphisms (SNPs) rs7726414 and rs244689 (near TCF7 and PPP2CA in 5q31.1) were selected as candidate independent associations from a large-scale study in a Han Chinese population consisting of 1047 cases and 1205 controls. Subsequently, 3509 cases and 8246 controls were genotyped in two further replication studies. We then investigated the SNPs' associations with SLE subphenotypes and gene expression in peripheral blood mononuclear cells. RESULTS: Highly significant associations with SLE in the Han Chinese population were detected for SNPs rs7726414 and rs244689 by combining the genotype data from our previous GWAS and two independent replication cohorts. Further conditional analyses indicated that these two SNPs contribute to disease susceptibility independently. A significant association with SLE, age at diagnosis < 20 years, was found for rs7726414 (P = 0·001). The expression levels of TCF7 and PPP2CA messenger RNA in patients with SLE were significantly decreased compared with those in healthy controls. CONCLUSIONS: This study found evidence for multiple associations with SLE in 5q31.1 at genome-wide levels of significance for the first time in a Han Chinese population, in a combined genotype dataset. These findings suggest that variants in the 5q31.1 locus not only provide novel insights into the genetic architecture of SLE, but also contribute to the complex subphenotypes of SLE.

Single cell transcriptome profiling revealed differences in gene expression during oocyte maturation in Haimen white goats.

Juvenile in vitro embryo transfer is an important animal reproductive technology that can shorten the generation interval of livestock, explore the reproductive potential of dams with excellent genetic traits, accelerate genetic progress and production efficiency of the herd, and provide a wealth of genetic resources for livestock breeding. However, oocytes from kids do not develop as well as those from female goats during in vitro maturation. To identify differences during different stages of oocyte maturation, we used single cell transcriptome sequencing to compare gene expression in mature oocytes from kids and female goats. We identified 1086 differentially expressed genes in mature oocytes from kids and female goats. Of these, we observed upregulated expression in 355 genes and downregulated expression in 435 genes. The differentially expressed genes were involved in a total of 245 different pathways; of which 30 were significant (P ≤ 0.05). We used real-time quantitative polymerase chain reaction to screen and verify the expression of five genes specifically involved in oocyte maturation (MOS, RPS6KA1, CPEB1, ANAPC13, and CDK1). Further study of these genes will be of great importance for improving the reproductive performance of Haimen white goats.

Genetic diversity and taxonomic status of Gymnocypris chilianensis based on the mitochondrial DNA cytochrome b gene.

In order to study the genetic diversity and taxonomic status of Gymnocypris chilianensis on a molecular level, the mitochondrial DNA cytochrome b gene was sequenced for 74 individuals of G. chilianensis from two locations (Heihe River and Shule River) and 42 individuals of its affinis species Gymnocypris przewalskii. Analyses of genetic diversity and sequence differences were conducted for these samples, combined with the analysis of 30 homologous sequences of another affinis species Gymnocypris eckloni, which were downloaded from GenBank. The results showed that both the haplotype diversity (h = 0.9820) and nucleotide diversity (π= 0.0039) of the Shule River G. chilianensis were lower than the other populations, thus, the Shule River G. chilianensis should be prioritized for protection because of its lower genetic diversity level. The results of sequence analysis showed that the genetic distance between the Heihe River G. chilianensis population and the Shule River G. chilianensis population was 0.0064, and the genetic distance between these two populations and the G. przewalskii population was 0.0838 and 0.0810, respectively. The genetic distance between the two G. chilianensis populations and the G. eckloni population was 0.0805 and 0.0778, respectively. Analysis of sequence differences indicates that G. chilianensis is sufficiently diverged from G. przewalskii and G. eckloni to the extent that it has reached species level, thus, G. chilianensis can be considered an independent species of Gymnocypris.

HTRA3 is reduced in ovarian cancers regardless of stage.

Ovarian cancer is the leading cause of death in gynaecological cancers. The high temperature requirement factor A3 (HtrA3) is involved in the pathogenesis of ovarian cancer. In this study we investigated whetherHtrA3 protein levels were altered in subtypes of ovarian cancer and whether HtrA3 down-regulation was associated with peritoneal metastasis. Ovarian cancer tissues from 89 patients were analyzed by immunohistochemistry. The levels of HtrA3 protein were lower in all subtypes of ovarian cancer and the lowest levels of HtrA3 were in epithelial ovarian cancer. The down-regulation of HtrA3 levels was not correlated with peritoneal metastasis of epithelial ovarian cancer.

Extratemporal abnormalities of brain parenchyma in young adults with temporal lobe epilepsy: a diffusion tensor imaging study.

AIM: To examine extratemporal abnormalities of the cerebral parenchyma in young adult temporal lobe epilepsy (TLE) patients using diffusion tensor imaging (DTI). MATERIALS AND METHODS: The study comprised 20 adults with unilateral TLE and 20 controls. The fractional anisotropy (FA), apparent diffusion coefficient (ADC), parallel eigenvalue (λ∥), and perpendicular eigenvalue (λ⊥) were calculated in the regions of interest (ROIs) using a 3 T MRI system. ROIs included the anterior/posterior limb of the internal capsule (AIC/PIC), external capsule (EC), head of caudate nucleus (HCN), lenticular nucleus (LN), thalamus (TL), and genu/body/splenium of the corpus callosum (GCC/BCC/SCC). RESULTS: Compared to controls, TLE patients showed lower FA in all ROIs; higher ADC in bilateral ECs, HCNs, TLs, and BCC; lower λ∥ in the ipsilateral LN and bilateral AICs, TL, and GCC; and higher λ⊥ in all ROIs except the bilateral PICs. In TLE patients, the ipsilateral TL had decreased FA compared with the contralateral TL. Pearson correlation analysis revealed a negative correlation between the ADC of the GCC and the age at onset of epilepsy; the λ∥ of the ipsilateral PIC and age at onset of epilepsy; the λ⊥ of the contralateral AIC and duration of epilepsy, respectively; and a positive correlation between the ADC of the GCC and the duration of epilepsy and the λ⊥ of the GCC and the duration of epilepsy, respectively. CONCLUSION: The study revealed bilateral extratemporal abnormalities in young adult TLE patients compared with controls. In addition, TLE patients with younger age at onset or longer duration of epilepsy may have more serious extratemporal changes.

Gene-gene interactions in IL23/Th17 pathway contribute to psoriasis susceptibility in Chinese Han population.

BACKGROUND: Psoriasis is a common chronic inflammatory skin disease. IL23/Th17 is a newly confirmed pathway in psoriasis. OBJECTIVE: To investigate the gene-gene interactions in IL23/Th17 pathway underlying psoriasis. METHODS: A total of 299 single-nucleotide polymorphisms from 11 genes in IL23/Th17 pathway were genotyped on 1139 patients with psoriasis and 1694 controls. Multifactor dimensionality reduction and logistic regression algorithms were applied to explore the gene-gene interactions. RESULTS: We found that there were a three-way interaction among IL21, CCR4 and TNF(χ(2) = 5.02(1), P = 0.025) and three pair-wise gene-gene interactions between IL12RB1 and CCR4(χ(2) = 11.66(4), P = 0.0201), IL22 and CCR4 (χ(2) = 11.97(4), P = 0.0176), IL12RB1 and IL6 (χ(2) = 7.31(1), P = 0.0069) in psoriasis. CONCLUSIONS: Our results might be helpful for explaining the missing heritability of the psoriasis due to epistasis and provide a deep insight into the important role of the IL23/Th17 pathway in the pathogenesis of psoriasis.

Different cellular p16(INK4a) localisation may signal different survival outcomes in head and neck cancer.

BACKGROUND: Recently, the management of head and neck squamous cell carcinoma (HNSCC) has focused considerable attention on biomarkers, which may influence outcomes. Tests for human papilloma infection, including direct assessment of the virus as well as an associated tumour suppressor gene p16, are considered reproducible. Tumours from familial melanoma syndromes have suggested that nuclear localisation of p16 might have a further role in risk stratification. We hypothesised p16 staining that considered nuclear localisation might be informative for predicting outcomes in a broader set of HNSCC tumours not limited to the oropharynx, human papilloma virus (HPV) status or by smoking status. METHODS: Patients treated for HNSCC from 2002 to 2006 at UNC (University of North Carolina at Chapel Hill) hospitals that had banked tissue available were eligible for this study. Tissue microarrays (TMA) were generated in triplicate. Immunohistochemical (IHC) staining for p16 was performed and scored separately for nuclear and cytoplasmic staining. Human papilloma virus staining was also carried out using monoclonal antibody E6H4. p16 expression, HPV status and other clinical features were correlated with progression-free (PFS) and overall survival (OS). RESULTS: A total of 135 patients had sufficient sample for this analysis. Median age at diagnosis was 57 years (range 20-82), with 68.9% males, 8.9% never smokers and 32.6% never drinkers. Three-year OS rate and PFS rate was 63.0% and 54.1%, respectively. Based on the p16 staining score, patients were divided into three groups: high nuclear, high cytoplasmic staining group (HN), low nuclear, low cytoplasmic staining group (LS) and high cytoplasmic, low nuclear staining group (HC). The HN and the LS groups had significantly better OS than the HC group with hazard ratios of 0.10 and 0.37, respectively, after controlling for other factors, including HPV status. These two groups also had significantly better PFS than the HC staining group. This finding was consistent for sites outside the oropharynx and did not require adjustment for smoking status. CONCLUSION: Different p16 protein localisation suggested different survival outcomes in a manner that does not require limiting the biomarker to the oropharynx and does not require assessment of smoking status.

A single-nucleotide polymorphism rs4639966 in 11q23.3 is associated with clinical features of systemic lupus erythematosus in the Chinese population.

In our previous genome-wide association study (GWAS), we identified an association signal of the single-nucleotide polymorphism (SNP) rs4639966 (p = 1.25 × 10(-16), odds ratio [OR] = 1.29) within 11q23.3. The aim of this study was to investigate its relationship with disease subphenotypes, including renal nephritis, photosensitivity, antinuclear antibody (ANA), age at diagnosis, malar rash, discoid rash, immunological disorder, oral ulcer, hematological disorder, neurological disorder, serositis, arthritis and vasculitis. In this study, we used 4199 cases and 8255 controls from our previous GWAS to explore the association between 11q23.3 with subphenotypes of systemic lupus erythematosus (SLE). Data were analyzed with PLINK 1.07 software. Significant associations were found for the SNP rs4639966 of 11q23.3 with SLE of age at diagnosis <20 years (OR = 1.18, p = 0.0049), malar rash (OR = 1.13, p = 0.01) and vasculitis (OR = 1.17, p = 0.02). The study suggested that 11q23.3 might not only play important roles in the development of SLE, but also contribute to the complex phenotypes of SLE.

Heritability of metabolic syndrome traits among healthy younger adults: a population based study in China.

OBJECTIVE To estimate heritability of metabolic syndrome traits among healthy younger adults in a human population in China, and examine potential sex differences in heritability and parental effect on metabolic syndrome traits. METHODS Using offspring-parent regression, we estimated heritability (h(2)) of metabolic syndrome traits based on 452 child-parent triads identified from a population based random survey on metabolic syndrome among people over 15 years of age in Guangzhou, China. RESULTS Body mass index (BMI), cholesterol, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG) and waist circumference (waist-C) were more heritable (h(2), 0.42-0.545), whereas systolic blood pressure (SBP), diastolic blood pressure (DBP), and triglycerides (TG) were less heritable (h(2), 0.14-0.28). Sons had pronounced increases in heritability for all traits over daughters, particularly for cholesterol (0.653 vs 0.356), FPG (0.602 vs 0.313), LDL-C (0.521 vs 0.329), and TG (0.395 vs 0.187). Offspring-mother seemed to have a higher heritability in every trait except FPG (0.67 vs 0.794) than offspring-father, most notably for DBP (0.308 vs 0.122), SBP (0.288 vs 0.146), TG (0.387 vs 0.239) and waist-C (0.581 vs 0.354). CONCLUSION We estimated the heritability of metabolic syndrome traits in a human population based on a unique population based offspring-parent sample from China, and found important evidence that the maternal and paternal effects on these traits are different and the sex difference in heritability is pronounced.

Clinical application of flash spiral mode of high-pitch dual source CT in carotid, cardiac and cerebral vessels combined one-stop angiography.

OBJECTIVE: This study aims to evaluate the clinical application value of flash spiral mode of high-pitch dual source CT in carotid, cardiac and cerebral vessels combined one-stop imaging. PATIENTS AND METHODS: A total of 100 consecutive patients were given carotid, cardiac and cerebral vessels combined one-stop imaging at flash spiral mode of high-pitch dual source CT. 27 patients received DSA examination of carotid and cerebral vessels, and 38 patients received digital subtraction angiography (DSA) examination of the coronary artery at the same time. Carotid, cardiac and cerebral vessels combined one-stop imaging was compared with "golden standard", DSA image. RESULTS: The overall satisfaction rate of coronary arteries, extracranial segment of the carotid artery (CA-E), intracranial segment of the carotid artery (CA-I), and cerebral vessels (anterior, middle, and posterior cerebral artery) were 93%, 99%, 95% and 97% respectively, and the positive rate of hemadostenosis was consistent with DSA. The kappa value indicating consistency of cerebral, carotid and coronary artery vessels was 0.78382, 0.80654, 0.82398, respectively. CONCLUSIONS: The method of carotid, cardiac and cerebral vessels combined one-stop imaging by flash spiral mode of high-pitch dual source CT can provide high image quality. It comprehensively evaluates stenosis of carotid, cardiac and cerebral vessels, which is of great importance for early intervention in harmful events of the cardia and cerebrovascular disorder.

TNIP1, SLC15A4, ETS1, RasGRP3 and IKZF1 are associated with clinical features of systemic lupus erythematosus in a Chinese Han population.

Systemic lupus erythematosus (SLE) is an autoimmune disease with heterogeneous clinical manifestations influenced by genetic and environmental factors. Five novel susceptibility genes (TNIP1, SLC15A4, ETS1, RasGRP3 and IKZF1) for SLE have been identified in a recent genome-wide association study of a Chinese Han population. This study investigated their relationships with disease subphenotypes, including renal nephritis, photosensitivity, antinuclear antibody (ANA), age at diagnosis, malar rash, discoid rash, immunological disorder, oral ulcer, hematological disorder, neurological disorder, serositis, arthritis and vasculitis. Significant associations were found for the single nucleotide polymorphism rs10036748 of TNIP1 with photosensitivity (odds ratio (OR) = 0.87, p = 0.01) and vasculitis (OR = 1.18, p = 0.04); rs10847697 of SLC15A4 with discoid rash (OR = 1.18, p = 0.02); rs6590330 of ETS1 with SLE of age at diagnosis <20 years (OR = 1.24, p = 8.91 x 10(-5)); rs13385731 of RasGRP3 with malar rash (OR = 1.20, p = 0.01), discoid rash (OR = 0.78, p = 0.02) and ANA (OR = 0.72, p = 0.004); rs4917014 of IKZF1 with renal nephritis (OR = 1.13, p = 0.02) and malar rash (OR = 0.83, p = 0.00038), respectively. The study suggested that these susceptibility genes might not only play important roles in the development of SLE, but also contribute to the complex phenotypes of SLE.

Spatiotemporal evolution of Poiseuille-Rayleigh-Bénard flows in binary fluids with Soret effect under initial pulselike disturbances.

The spatiotemporal evolution of Poiseuille-Rayleigh-Bénard flows in binary fluids with Soret effect is investigated by carrying out fully nonlinear two-dimensional numerical simulations initiated by a pulselike disturbance. The traveling wave packets for positive as well as negative separation factors psi are obtained numerically for ethanol-water-like mixtures (Prandtl number Pr=10 , Lewis number Le=0.01) and selected combinations of Rayleigh and Reynolds numbers at psi=0.01, 0.1 and psi=-0.1. The characteristics of the wave fronts and the transitions observed between absolute and convective instabilities when changing the parameters are compared with the results previously obtained by linear spatiotemporal stability analysis. The simulations are in very good agreement with the stability results, which confirms the validity of both approaches. Finally, in order to characterize the possible interaction between the two wave packets of the so-called downstream and upstream modes for psi<0, the spatiotemporal stability analysis is used to detect a boundary curve in the (Re, Ra) parameter region beyond which the two wave packets will never completely separate. Numerical simulations illustrate the different evolutions of the wave packets on both sides of this boundary.

Autoinhibition of IL-15 expression in KC cells is ERK1/2 and PI3K dependent.

Interleukin (IL)-15 is a proinflammatory cytokine and plays a key role in many diseases, including psoriasis. Although its signal transduction pathways in keratinocytes (KC) have been partially elucidated, the effects of IL-15 on expression of IL-15, IL-6 and TNF-alpha in KC are unknown. We have investigated the effects of IL-15 on the expression of the three genes in primary culture of KC by the real-time PCR, Western blot and ELISA. We observed that exogenous IL-15 suppressed the endogenous expression of IL-15, decreased the expression of IL-6 at mRNA and protein levels in KC. The inhibition was blocked by anti-IL-15 monoclonal antibody and by inactive IL-15, I50D mutant IL-15. In contrast, IL-15 increased TNF-alpha transcription in these cells. Mechanistic studies demonstrated that the auto-regulation of IL-15 expression was dependent on activity of ERK1/2 and PI3K. Our studies suggest that there is an auto-inhibitory mechanism controlling cellular IL-15 levels.

Linear temporal and spatiotemporal stability analysis of two-layer falling films with density stratification.

A detailed temporal and spatiotemporal stability analysis of two-layer falling films with density and viscosity stratification is performed by using the Chebyshev collocation method to solve the full system of linear stability equations. From the neutral curves Re(k) for the surface mode and the interface mode of instability, obtained for different density ratios gamma of the upper layer to the lower layer, it is found that smaller density ratios make the surface mode and the short-wave interface mode much more stable, and can even make the short-wave interfacial instability disappear. Moreover, through the study of the local growth rates of the spatiotemporal instability as a function of the ray velocity V , it is found that for not too small incline angles like theta=0.2, the two-layer flow is always convectively unstable, and there is a transition between long- and short-wave instabilities which is determined by the Briggs-Bers collision criterion. Due to the existence of the absolute Rayleigh-Taylor instability for gamma>0 and theta=0, a transition from convective to absolute instability can be detected at small incline angles, and the corresponding boundary curves are plotted for different Reynolds numbers, viscosity ratios, and incline angles. It is found that there exists a limit Reynolds number above which the two-layer film flow can only be convectively unstable for a fixed small incline angle. The spatial amplification properties of the convective waves are finally presented for both surface and interface modes.

Expression of three proteins in endometrioid adenocarcinoma and their significance in clinical nursing, diagnosis and treatment.

OBJECTIVE: This study aimed to investigate the role of NEDD9 (neural precursor cell expressed developmentally down-regulated 9), BCAR1/P130CAS (BCAR1/P130 Crk-associated substrate) and paxillin in predicting the prognosis of endometrioid adenocarcinoma (EA), so as to guild the nursing of EA. PATIENTS AND METHODS: A total of 65 patients who visited Affiliated Hongqi Hospital of Mudanjiang Medical University between June 2015 and June 2017 were enrolled. They underwent gynecological surgery and had their EA confirmed by pathology, and they were assigned to the EA group. All EA tissues were sampled and archived in paraffin blocks. In addition, 40 specimens of atypical endometrial hyperplasia (EAH) (the EAH group) and 40 specimens of normal proliferative endometria with benign uterine fibroids (the EN group) were selected as controls. The protein levels of NEDD9, BCAR1/P130CAS, and paxillin in each group were then detected by immunohistochemical staining. RESULTS: The expression of the three proteins in the EA group and EAH group was significantly higher than that in the EN group, and their expression was significantly correlated with the clinical stage, histological grade and lymph node metastasis of EA. In addition, the expression of NEDD9, BCAR1/P130CAS, and paxillin in the EA group was positively correlated with each other. CONCLUSIONS: BCAR1/P130CAS and paxillin interact with NEDD9 to participate in the growth and migration of EA cells. Therefore, their proteins can be used as biomarkers for the diagnosis, treatment, and prognosis of EA.