Simultaneous enantioselective determination of omeprazole, rabeprazole, lansoprazole, and pantoprazole enantiomers in human plasma by chiral liquid chromatography-tandem mass spectrometry.

Proton pump inhibitors, including omeprazole, rabeprazole, lansoprazole, and pantoprazole, achieved simultaneous enantioselective determination in the human plasma by chiral liquid chromatography-tandem mass spectrometry. The four corresponding stable isotope-labeled proton pump inhibitors were adopted as the internal standards. Each enantiomer and the internal standards were extracted with acetonitrile containing 0.1% ammonia, then separated with a Chiralpak IC column (5 µm, 4.6 mm × 150 mm) within 10 min. The mobile phase was composed of acetonitrile-ammonium acetate (10 mM) containing 0.2% acetic acid (50:50, v/v). To quantify all enantiomers, an API 4000 tandem mass spectrometer was used, and multiple reaction monitoring transitions were performed on m/z 360.1→242.1, 384.1→200.1, 370.1→252.1, and 346.1→198.1, respectively. No significant matrix effect was observed for all analytes. The calibration curve for all enantiomers were linear from 1.25 to 2500 ng/mL. The precisions for intra- and inter-run were < 14.2%, and the accuracy fell in the interval of -5.3 to 8.1%. Stability of samples was confirmed under the storage and processing conditions. The developed method was also suitable for separation and determination of ilaprazole enantiomers. The validated method combining the equilibrium dialysis method was applied to the protein binding ratio studies of four pairs proton pump inhibitor enantiomers in human plasma.

Stereoselective pharmacokinetics of (R)-(+)- and (S)-(-)-rabeprazole in human using chiral LC-MS/MS after administration of rabeprazole sodium enteric-coated tablet.

Rabeprazole is an effective proton pump inhibitor to treat acid-related diseases. To achieve the simultaneous determination of rabeprazole enantiomers in human plasma, a chiral LC-MS/MS method was developed and validated. Acetonitrile including 0.1% ammonium were used as protein precipitating agent. Analytes were separated within 8 minutes on a Chiralpak IC column (4.6 mm × 150 mm, 5 µm). The mobile phase was 10 mM ammonium acetate including 0.2% acetic acid-acetonitrile (35:65, v/v). An API 4000 mass spectrometer was used as detector for the analysis, and the multiple reactions monitoring transitions of m/z 360.1 â†’ 242.2 and 346.1 â†’ 198.1 were opted for quantifying rabeprazole enantiomers and internal standard. Matrix effects were not apparent for each enantiomer and internal standard (esomeprazole), the calibration curves were linear over the concentration of 0.500 to 400 ng·mL-1 , the intra-run precisions were below 5.4%, the inter-run precisions were below 9.9%, and the accuracy was between -9.2% and 9.3%. There was no chiral inversion observed during sample storage, preparation procedure, and analysis, demonstrating that analytes were stable in this study. This method was applied to the stereoselective pharmacokinetic study of (R)-(+)- and (S)-(-)-rabeprazole after oral administration of 10-mg rabeprazole sodium enteric-coated tablet in healthy Chinese subjects.

Acculturation and Post-Migration Stress in Middle-Aged Chinese Immigrant Women in Philadelphia: Variation between the Fujianese and the non-Fujianese women.

The experience of acculturation in Chinese immigrant women from the rural coastal province of Fujian has not been well studied despite of their growing numbers in American cities. This exploratory study is an attempt to examine the experience of acculturation and post-migration stress in Fujianese immigrant women as compared to those from other parts of China. The study is based on a convenience sample 240 Fujianese and 162 non-Fujianese Chinese immigrant women living in Philadelphia.Results from bivariate analyses showed that the variation in demographic characteristics between Fujianese and non-Fujianese women was marginal; that all Chinese women in this study reported experiencing a unidimensional process of acculturation and a domain-generic model of acculturation; and that the Fujianese women showed a higher level of post-migration stress than the non-Fujianese women. In multiple regression controlling for demographic characteristics and including all the women in our sample, more acculturated women reported a higher level of post-migration stress. However, separate multiple regression analyses for Fujianese and non-Fujianese women revealed a different pattern of post-migration stress models. The findings suggest the importance of further research to understand acculturation and post-migration stress among Fujianese immigrant women.

Cultural orientation in Southeast Asian American young adults.

The study assessed cultural orientation in a group of 188 Southeast Asian American American-born and early-arriving (by age 5) young adults using the General Ethnicity Questionnaire--Southeast Asian and American versions. The sample included 98 Vietnamese, 45 Cambodian, and 45 Hmong college students in California. As hypothesized and in support of the bidimensional model, overall orientation to ethnic and American cultures were independent of each other, and predicted, respectively, by ethnic and American cultural domains. The three ethnic groups did not vary on level of cultural orientation, but their endorsement of ethnic and American cultures differed across domains. Specifically, they enjoyed American mass media and cultural activities more than ethnic media and activities, and were more fluent in English than their ethnic language. However, they expressed greater ethnic pride and a stronger overall Southeast Asian orientation than American pride and orientation.

The longitudinal effect of intergenerational gap in acculturation on conflict and mental health in Southeast Asian American adolescents.

This longitudinal study examined the intergenerational gap in acculturation, subsequent conflict, and their mental health consequences in Southeast Asian American adolescents. It was hypothesized that perceived intergenerational discrepancy in acculturation during early adolescence would predict intergenerational conflict in late adolescence, which, in turn, would increase depressive symptomatology in late adolescence. Using data from the Children of Immigrants Longitudinal Study (A. Portes & R. G. Rumbaut, 2001; R. G. Rumbaut, 1994), 490 Southeast Asian American adolescents in 8th and 9th grades completed surveys and again 3 years later. The results supported the hypothesis and showed that intergenerational/intercultural conflict fully mediated the longitudinal effect of perceived intergenerational discrepancy in acculturation on depressive symptomatology. Recommendations for community-based interventions for both parents and youth are offered.

Predictors of depressive symptoms in Chinese American college students: parent and peer attachment, college challenges and sense of coherence.

Based on Antonovsky's salutogenic model, the authors hypothesized that sense of coherence would mediate the effects of parent and peer attachment and college challenges on depressive symptoms as well as moderate the relationship between college challenges and depressive symptoms in Chinese Americans. To test our hypotheses, 353 Chinese American college students completed paper-pencil measures. Supporting our hypotheses, sense of coherence fully mediated the effects of parent and peer attachment on depressive symptom level and served as a partial mediator and moderator of the effect of college challenges on depressive symptoms. Implications of the study findings for promoting the mental health of Chinese American students are discussed.

Analysis of clinical effect of blood purification on acute benzene-based thinner poisoning / 中华劳动卫生职业病杂志

Objective: To investigate the clinical significance of blood purification on changes in serum toxicant concentration and prognosis of acute benzene-based thinner poisoning. Methods: A total of 44 patients with acute benzene-based thinner poisoning admitted to the emergency department of Characteristic Medical Center of Armed Police from August 2013 to August 2020 were collected and divided into a blood purification group (24 cases) and a conventional treatment group (20 cases) , the general data, toxicant concentrations and prognosis of the two groups of patients were analyzed, and logistic regression analysis was performed on the influencing factors of the prognosis to explore the clinical effect of blood purification. Results: The concentration of poisons in the blood purification group at 24 hours after treatment was significantly lower than that in the conventional treatment group (t=6.76, P<0.001) , and the reduction in the concentration of poisons was significantly higher than that in the conventional treatment group (t=3.33, P=0.002) . The overall improvement rate in the blood purification group was 91.7% (22/24) , which was higher than that in the conventional treatment group (60.0%, 12/20) . Logisitic regression analysis showed that blood purification treatment method was the main factor affecting the prognosis of patients (OR=7.605×10(-5), 95%CI: 6.604×10(-8)-0.087, P=0.008) , and the toxic dose was a synergistic effect on the prognosis of patients factor (OR=1.038, 95%CI: 1.008-1.068, P=0.011) . Conclusion: Early blood purification treatment in patients with acute benzene-based thinner poisoning can rapidly reduce blood toxin concentration, avoid disease progression, and ultimately improve patient prognosis.

Inventory of college challenges for ethnic minority students: psychometric properties of a new instrument in Chinese Americans.

The Inventory of College Challenges for Ethnic Minority Students (ICCEMS) is a newly developed instrument that assesses challenges faced by ethnic minority college students across a range of cultural, academic, social, and practical domains. The present study tested the ICCEMS among Chinese American students in an attempt to identify its factor structure and assess its psychometric properties. A total of 13 factor domains emerged. The Cronbach's alpha and 1-month test-retest reliability of the subscales and the overall scale supported their reliability. Both criterion and construct validities were also demonstrated. Chinese American college students faced the greatest challenges in terms of unclear career direction and academic demands.

The effect of cross-cultural living on personality: assimilation and accommodation among Taiwanese young adults in the United States.

Cross-cultural living represents a transition that entails significant psychological disequilibration, to which individuals may respond with assimilation (retention of existing schemas) or accommodation (creation of new schemas; J. Block, 1982). Using the 3 California Psychological Inventory vector scales, the author assessed the personality of 97 Taiwanese young adults studying in the United States prearrival and at 1 and 2 years postarrival. The study found support for both assimilation (stability) and accommodation (change) in personality secondary to cross-cultural living.