RESUMEN
Taxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little is known about the precise distribution of taxoids and the transcriptional regulation of taxoid biosynthesis across Taxus stems. Here, we used MALDI-IMS analysis to visualize the taxoid distribution across Taxus mairei stems and single-cell RNA sequencing to generate expression profiles. A single-cell T. mairei stem atlas was created, providing a spatial distribution pattern of Taxus stem cells. Cells were reordered using a main developmental pseudotime trajectory which provided temporal distribution patterns in Taxus stem cells. Most known taxol biosynthesis-related genes were primarily expressed in epidermal, endodermal, and xylem parenchyma cells, which caused an uneven taxoid distribution across T. mairei stems. We developed a single-cell strategy to screen novel transcription factors (TFs) involved in taxol biosynthesis regulation. Several TF genes, such as endodermal cell-specific MYB47 and xylem parenchyma cell-specific NAC2 and bHLH68, were implicated as potential regulators of taxol biosynthesis. Furthermore, an ATP-binding cassette family transporter gene, ABCG2, was proposed as a potential taxoid transporter candidate. In summary, we generated a single-cell Taxus stem metabolic atlas and identified molecular mechanisms underpinning the cell-specific transcriptional regulation of the taxol biosynthesis pathway.
Asunto(s)
Taxoides , Taxus , Taxoides/metabolismo , Transcriptoma , Taxus/genética , Taxus/metabolismo , Paclitaxel , Espectrometría de MasasRESUMEN
Uterine corpus endometrial carcinoma (UCEC) is the most common cancer of the female reproductive tract. The overall survival of advanced and recurrent UCEC patients is still unfavourable nowadays. It is urgent to find a predictive biomarker and block tumorgenesis at an early stage. Plant homeodomain finger protein 6 (PHF6) is a key player in epigenetic regulation, and its alterations lead to various diseases, including tumours. Here, we found that PHF6 expression was upregulated in UCEC tissues compared with normal tissues. The UCEC patients with high PHF6 expression had poor survival than UCEC patients with low PHF6 expression. PHF6 mutation occurred in 12% of UCEC patients, and PHF6 mutation predicted favourable clinical outcome in UCEC patients. Depletion of PHF6 effectively inhibited HEC-1-A and KLE cell proliferation in vitro and decreased HEC-1-A cell growth in vivo. Furthermore, high PHF6 level indicated a subtype of UCECs characterized by low immune infiltration, such as CD3+ T-cell infiltration. While knockdown of PHF6 in endometrial carcinoma cells increased T-cell migration by promoting IL32 production and secretion. Taken together, our findings suggested that PHF6 might play an oncogenic role in UCEC patients. Thus, PHF6 could be a potential biomarker in predicting the prognosis of UCEC patients. Depletion of PHF6 may be a novel therapeutic strategy for UCEC patients.
Asunto(s)
Carcinoma Endometrioide , Neoplasias Endometriales , Femenino , Humanos , Epigénesis Genética , Linfocitos T/metabolismo , Recurrencia Local de Neoplasia/genética , Neoplasias Endometriales/patología , Útero/metabolismo , Carcinoma Endometrioide/genética , Proteínas Represoras/genéticaRESUMEN
Ferroptosis is a newly discovered form of programmed cell death, which has unique biological effects on metabolism and redox biology. In this study, the prognostic value of ferroptosis-related genes was investigated in lower-grade gliomas (LGG). We downloaded the ferroptosis-related genes from the FerrDb dataset. Univariate Cox and LASSO regression analyses were applied to identify genes correlated with overall survival (OS). Subsequently, 12 ferroptosis-related genes were screened to establish the prognostic signature using stepwise multivariate Cox regression. According to the median value of risk scores, patients were divided into low- and high-risk subgroups. The Kaplan-Meier curves showed the high-risk group had a lower OS. The predictive power of the risk model was validated using the CGGA. Functional analysis revealed that the terms associated with plasma membrane receptor complex, immune response and glutamate metabolic process were primarily related to the risk model. Moreover, we established a nomogram that had a strong forecasting ability for the 1-, 3- and 5-year OS. In addition, we compared the risk scores between different clinical features. We also detected infiltration of macrophages and monocytes in different subgroups. Overall, our study identified the prognostic signature of 12 ferroptosis-related genes, which has the potential to predict the prognosis of LGG.
Asunto(s)
Biomarcadores de Tumor , Neoplasias Encefálicas/genética , Ferroptosis/genética , Perfilación de la Expresión Génica , Regulación Neoplásica de la Expresión Génica , Glioma/genética , Transcriptoma , Algoritmos , Neoplasias Encefálicas/mortalidad , Neoplasias Encefálicas/patología , Biología Computacional/métodos , Bases de Datos de Ácidos Nucleicos , Glioma/mortalidad , Glioma/patología , Humanos , Nomogramas , Curva ROC , Reproducibilidad de los ResultadosRESUMEN
Taxus stem barks can be used for extraction of paclitaxel. However, the composition of taxoids across the whole stem and the stem tissue-specificity of paclitaxel biosynthesis-related enzymes remain largely unknown. We used cultivated Taxus media trees for analyses of the chemical composition and protein of major stem tissues by an integrated metabolomic and proteomic approach, and the role of TmMYB3 in paclitaxel biosynthesis was investigated. The metabolomic landscape analysis showed differences in stem tissue-specific accumulation of metabolites. Phytochemical analysis revealed that there is high accumulation of paclitaxel in the phloem. Ten key enzymes involved in paclitaxel biosynthesis were identified, most of which are predominantly produced in the phloem. The full-length sequence of TmMYB3 and partial promoter sequences of five paclitaxel biosynthesis-related genes were isolated. Several MYB recognition elements were found in the promoters of TBT, DBTNBT and TS. Further in vitro and in vivo investigations indicated that TmMYB3 is involved in paclitaxel biosynthesis by activating the expression of TBT and TS. Differences in the taxoid composition of different stem tissues suggest that the whole stem of T. media has potential for biotechnological applications. Phloem-specific TmMYB3 plays a role in the transcriptional regulation of paclitaxel biosynthesis, and may explain the phloem-specific accumulation of paclitaxel.
Asunto(s)
Paclitaxel/biosíntesis , Floema/metabolismo , Proteínas de Plantas/metabolismo , Tallos de la Planta/metabolismo , Taxus/metabolismo , Factores de Transcripción/metabolismo , Regulación de la Expresión Génica de las Plantas/genética , Redes y Vías Metabólicas , Metabolómica , Proteínas de Plantas/fisiología , Regiones Promotoras Genéticas , Proteómica , Factores de Transcripción/fisiologíaRESUMEN
BACKGROUND: Taxol is an efficient anticancer drug accumulated in Taxus species. Pseudotaxus chienii is an important member of Taxaceae, however, the level of six taxoids in P. chienii is largely unknown. RESULTS: High accumulation of 10-DAB, taxol, and 7-E-PTX suggested that P. chienii is a good taxol-yielding species for large-scale cultivation. By the omics approaches, a total of 3,387 metabolites and 61,146 unigenes were detected and annotated. Compared with a representative Taxus tree (Taxus yunnanensis), most of the differentially accumulated metabolites and differential expressed genes were assigned into 10 primary and secondary metabolism pathways. Comparative analyses revealed the variations in the precursors and intermediate products of taxol biosynthesis between P. chienii and T. yunnanensis. Taxusin-like metabolites highly accumulated in P. chienii, suggesting a wider value of P. chienii in pharmaceutical industry. CONCLUSIONS: In our study, the occurrence of taxoids in P. chienii was determined. The differential expression of key genes involved in the taxol biosynthesis pathway is the major cause of the differential accumulation of taxoids. Moreover, identification of a number of differentially expressed transcription factors provided more candidate regulators of taxol biosynthesis. Our study may help to reveal the differences between Pseudotaxus and Taxus trees, and promote resource utilization of the endangered and rarely studied P. chienii.
Asunto(s)
Vías Biosintéticas , Metaboloma , Metabolómica , Paclitaxel/biosíntesis , Plantas Medicinales/metabolismo , Especificidad de la Especie , Taxaceae/metabolismo , Especies en Peligro de Extinción , Variación GenéticaRESUMEN
KEY MESSAGE: We employed both metabolomic and transcriptomic approaches to explore the accumulation patterns of physalins, flavonoids and chlorogenic acid in Physalis angulata and revealed the genes associated with the biosynthesis of bioactive compounds under methyl-jasmonate (MeJA) treatment. Physalis angulata L. is an annual Solanaceae plant with a number of medicinally active compounds. Despite the potential pharmacological benefits of P. angulata, the scarce genomic information regarding this plant has limited the studies on the mechanisms of bioactive compound biosynthesis. To facilitate the basic understanding of the main chemical constituent biosynthesis pathways, we performed both metabolomic and transcriptomic approaches to reveal the genes associated with the biosynthesis of bioactive compounds under methyl-jasmonate (MeJA) treatment. Untargeted metabolome analysis showed that most physalins, flavonoids and chlorogenic acid were significantly upregulated. Targeted HPLC-MS/MS analysis confirmed variations in the contents of two important representative steroid derivatives (physalins B and G), total flavonoids, neochlorogenic acid, and chlorogenic acid between MeJA-treated plants and controls. Transcript levels of a few steroid biosynthesis-, flavonoid biosynthesis-, and chlorogenic acid biosynthesis-related genes were upregulated, providing a potential explanation for MeJA-induced active ingredient synthesis in P. angulata. Systematic correlation analysis identified a number of novel candidate genes associated with bioactive compound biosynthesis. These results may help to elucidate the regulatory mechanism underlying MeJA-induced active compound accumulation and provide several valuable candidate genes for further functional study.
Asunto(s)
Acetatos/farmacología , Ciclopentanos/farmacología , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Oxilipinas/farmacología , Physalis/efectos de los fármacos , Physalis/metabolismo , Proteínas de Plantas/metabolismo , Flavonoides/biosíntesis , Flavonoides/química , Metaboloma , Estructura Molecular , Reguladores del Crecimiento de las Plantas/farmacología , Proteínas de Plantas/genética , Raíces de Plantas/genética , Raíces de Plantas/metabolismo , ARN de Planta/genética , TranscriptomaRESUMEN
BACKGROUND: Taxol is an efficient anticancer drug; however, the accumulation of taxoids can vary hugely among Taxus species. The mechanism underlying differential accumulation of taxoids is largely unknown. Thus, comparative analysis of the transcriptomes in three Taxus species, including T. media, T. mairei and T. cuspidata, was performed. RESULTS: KEGG enrichment analysis revealed that the diterpenoid biosynthesis and cytochrome P450 pathways were significantly enriched in different comparisons. Differential expressions of these taxol biosynthesis related genes might be a potential explanation for the interspecific differential accumulation of taxol and its derivatives. Besides, the sequences of several MEP pathway-associated genes, such as DXS, DXR, MCT, CMK, MDS, HDS, HDR, IPPI, and GGPPS, were re-assembled based on independent transcriptomes from the three Taxus species. Phylogenetic analysis of these MEP pathway-associated enzymes also showed a high sequence similarity between T. media and T. cuspidata. Moreover, 48 JA-related transcription factor (TF) genes, including 10 MYBs, 5 ERFs, 4 RAPs, 3 VTCs, and 26 other TFs, were analyzed. Differential expression of these JA-related TF genes suggested distinct responses to exogenous JA applications in the three Taxus species. CONCLUSIONS: Our results provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species. The data give us an opportunity to reveal the mechanism underlying the variations in the taxoid contents and to select the highest-yielding Taxus species.
Asunto(s)
Perfilación de la Expresión Génica/métodos , Paclitaxel/biosíntesis , Taxus/genética , Taxus/metabolismo , Transcriptoma/genética , Taxoides/metabolismoRESUMEN
BACKGROUND: Trees of the genus Taxus are highly valuable medicinal plants with multiple pharmacological effects on various cancer treatments. Paclitaxel from Taxus trees is an efficient and widely used anticancer drug, however, the accumulation of taxoids and other active ingredients can vary greatly among Taxus species. In our study, the metabolomes of three Taxus species have been investigated. RESULTS: A total of 2246 metabolites assigned to various primary and secondary metabolic pathways were identified using an untargeted approach. Analysis of differentially accumulated metabolites identified 358 T. media-, 220 T. cuspidata-, and 169 T. mairei-specific accumulated metabolites, respectively. By searching the metabolite pool, 7 MEP pathway precursors, 11 intermediates, side chain products and derivatives of paclitaxel, and paclitaxel itself were detected. Most precursors, initiated intermediates were highly accumulated in T. mairei, and most intermediate products approaching the end point of taxol biosynthesis pathway were primarily accumulated in T. cuspidata and T. media. Our data suggested that there were higher-efficiency pathways to paclitaxel in T. cuspidata and T. media compared with in T. mairei. As an important class of active ingredients in Taxus trees, a majority of flavonoids were predominantly accumulated in T. mairei rather than T. media and T. cuspidata. The variations in several selected taxoids and flavonoids were confirmed using a targeted approach. CONCLUSIONS: Systematic correlativity analysis identifies a number of metabolites associated with paclitaxel biosynthesis, suggesting a potential negative correlation between flavonoid metabolism and taxoid accumulation. Investigation of the variations in taxoids and other active ingredients will provide us with a deeper understanding of the interspecific differential accumulation of taxoids and an opportunity to accelerate the highest-yielding species breeding and resource utilization.
Asunto(s)
Flavonoides/metabolismo , Metaboloma , Taxoides/metabolismo , Taxus/metabolismo , Redes y Vías Metabólicas , Metabolómica , Especificidad de la EspecieRESUMEN
BACKGROUND: Plants of the genus Taxus have attracted much attention owing to the natural product taxol, a successful anti-cancer drug. T. fuana and T. yunnanensis are two endangered Taxus species mainly distributed in the Himalayas. In our study, an untargeted metabolomics approach integrated with a targeted UPLC-MS/MS method was applied to examine the metabolic variations between these two Taxus species growing in different environments. RESULTS: The level of taxol in T. yunnanensis is much higher than that in T. fuana, indicating a higher economic value of T. yunnanensis for taxol production. A series of specific metabolites, including precursors, intermediates, competitors of taxol, were identified. All the identified intermediates are predominantly accumulated in T. yunnanensis than T. fuana, giving a reasonable explanation for the higher accumulation of taxol in T. yunnanensis. Taxusin and its analogues are highly accumulated in T. fuana, which may consume limited intermediates and block the metabolic flow towards taxol. The contents of total flavonoids and a majority of tested individual flavonoids are significantly accumulated in T. fuana than T. yunnanensis, indicating a stronger environmental adaptiveness of T. fuana. CONCLUSIONS: Systemic metabolic profiling may provide valuable information for the comprehensive industrial utilization of the germplasm resources of these two endangered Taxus species growing in different environments.
Asunto(s)
Metabolómica/métodos , Taxus/metabolismo , Especies en Peligro de Extinción , Flavonoides/metabolismo , Paclitaxel/metabolismo , Metabolismo Secundario , Taxoides/análisis , Taxoides/metabolismo , TibetRESUMEN
PURPOSE: Gliomas are destructive malignancies affecting mainly the central nervous system. Gliomas constitute around 50% of all the central nervous system tumors. The purpose of this study was to examine the anticancer activity of cycloartenol against the glioma U87 cells and to investigate the underlying mechanisms. METHODS: MTT and colony formation assay were used to determine the proliferation rate. Acridine orange/ethidium bromide (AO/EB) and annexin V/propidium iodide (PI) were used to determine apoptosis and cell cycle analysis was carried out by western blotting. Cell migration was checked by cell migration assay and immunoblotting was used for checking protein expressions. RESULTS: The results revealed that cycloartenol inhibited the proliferation and the colony formation potential of the glioma U87 cells in a concentration-dependent manner. The antiproliferative effects were found to be due to induction of Sub-G1 cell cycle arrest and triggering of apoptosis. These effects were found to be dose-dependent. Cycloartenol also caused significant alteration in the expression of Bax and Bcl-2. Furthermore, cycloartenol inhibited the migration of glioma cells and suppressed the phosphorylation of the p38 MAP kinase. CONCLUSION: These findings indicate that cycloartenol may prove beneficial in the treatment of glioma and warrants further investigation.
Asunto(s)
Apoptosis/efectos de los fármacos , Puntos de Control del Ciclo Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Glioma/patología , Fitosteroles/farmacología , Triterpenos/farmacología , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo , Glioma/tratamiento farmacológico , Glioma/metabolismo , Humanos , Fosforilación , Células Tumorales CultivadasRESUMEN
Hormesis, a biphasic dose-response phenomenon, which is characterized by stimulation of an end point at a low-dose and inhibition at a high-dose. In the present study we used human lungs fibroblast (HELF) cells as a test model to evaluate the role of oxidative stress (OS) in hormetic effects of non coplanar PCB 101. Results from 3-(4,5-dime-thylthiazol-2-yl)-2,5-diphenyltetrazo-lium bromide (MTT) assay indicated that PCB101 at lower concentrations (10(-5) to 10(-1) µg mL(-1) ) stimulated HELF cell proliferation and inhibited at high concentrations (1, 5, 10, and 20 µg mL(-1) ) in a dose- and time-dependent manner. Reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) (except 48 h) showed a significant increase at higher concentrations of PCB 101 than those at the lower concentrations with the passage of time. Antioxidant enzymes such as glutathione peroxidase (GSH-Px) exhibited decreasing trends in dose and time dependent manner. Lipid peroxidation assay resulted in a significant increase (P < 0.05) of MDA level in PCB 101-treated HELF cells compared with controls, suggesting that OS plays a key role in PCB 101-induced toxicity. Comet assay indicated a significant increase in genotoxicity at higher concentrations of PCB 101 exposure compared to lower concentrations. Overall, we found that HELF cell proliferation was higher at low ROS level and vice versa, which revealed activation of cell signaling-mediated hormetic mechanisms. The results suggested that PCB 101 has hormetic effects to HELF cells and these were associated with oxidative stress.
Asunto(s)
Estrés Oxidativo/efectos de los fármacos , Bifenilos Policlorados/toxicidad , Línea Celular , Proliferación Celular/efectos de los fármacos , Ensayo Cometa , Daño del ADN/efectos de los fármacos , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Fibroblastos/metabolismo , Glutatión Peroxidasa/metabolismo , Humanos , Peroxidación de Lípido/efectos de los fármacos , Pulmón/citología , Pulmón/metabolismo , Malondialdehído/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/metabolismoRESUMEN
Glioblastoma (GBM), the deadliest central nervous system cancer, presents a poor prognosis and scant therapeutic options. Our research spotlights OH2, an oncolytic viral therapy derived from herpes simplex virus 2 (HSV-2), which demonstrates substantial antitumor activity and favorable tolerance in GBM. The extraordinary efficacy of OH2 emanates from its unique mechanisms: it selectively targets tumor cells replication, powerfully induces cytotoxic DNA damage stress, and kindles anti-tumor immune responses. Through single-cell RNA sequencing analysis, we discovered that OH2 not only curtails the proliferation of cancer cells and tumor-associated macrophages (TAM)-M2 but also bolsters the infiltration of macrophages, CD4+ and CD8+ T cells. Further investigation into molecular characteristics affecting OH2 sensitivity revealed potential influencers such as TTN, HMCN2 or IRS4 mutations, CDKN2A/B deletion and IDO1 amplification. This study marks the first demonstration of an HSV-2 derived OV's effectiveness against GBM. Significantly, these discoveries have driven the initiation of a phase I/II clinical trial (ClinicalTrials.gov: NCT05235074). This trial is designed to explore the potential of OH2 as a therapeutic option for patients with recurrent central nervous system tumors following surgical intervention.
Asunto(s)
Neoplasias Encefálicas , Glioblastoma , Viroterapia Oncolítica , Virus Oncolíticos , Humanos , Virus Oncolíticos/genética , Glioblastoma/genética , Glioblastoma/terapia , Linfocitos T CD8-positivos , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/terapiaRESUMEN
Livestock wastewater has high estrogen activity because animal excreta contain estrogen. In the past, when biological technologies were applied to treat livestock wastewater, the removal efficiency of estrogen pollutants was always ignored. Therefore, the efficiency of estrogen removal by anaerobic/aerobic (A/O) treatment and by up flow anaerobic sludge blanket and step-fed sequencing batch reactor (UASB-SFSBR) treatment was investigated in the present study. The results showed that the A/O treatment had no significant estrogenic removal ability, whereas the removal rates of estrogen after UASB-SFSBR treatment reached approximately 78 %, as measured by liquid chromatography and tandem mass spectrometry. The estrogen concentration decreased from 31.5 ng/L to an undetectable level according to the yeast estrogen screen analysis. We found differences between the estrogen removal rates measured by the chemical assay and those measured using the bioassay. More attention must be paid to the removal of estrogen pollutants in livestock wastewater to reduce the environmental risk.
Asunto(s)
Estrógenos/análisis , Aguas del Alcantarillado/química , Porcinos , Eliminación de Residuos Líquidos/métodos , Aguas Residuales/química , Contaminantes Químicos del Agua/análisis , Animales , Reactores Biológicos , Cromatografía Liquida , Estrógenos/metabolismo , Ganado , Espectrometría de Masas , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/metabolismoRESUMEN
Taxus leaves provide the raw industrial materials for taxol, a natural antineoplastic drug widely used in the treatment of various cancers. However, the precise distribution, biosynthesis, and transcriptional regulation of taxoids and other active components in Taxus leaves remain unknown. Matrix-assisted laser desorption/ionization-mass spectrometry imaging analysis was used to visualize various secondary metabolites in leaf sections of Taxus mairei, confirming the tissue-specific accumulation of different active metabolites. Single-cell sequencing was used to produce expression profiles of 8846 cells, with a median of 2352 genes per cell. Based on a series of cluster-specific markers, cells were grouped into 15 clusters, suggesting a high degree of cell heterogeneity in T. mairei leaves. Our data were used to create the first Taxus leaf metabolic single-cell atlas and to reveal spatial and temporal expression patterns of several secondary metabolic pathways. According to the cell-type annotation, most taxol biosynthesis genes are expressed mainly in leaf mesophyll cells; phenolic acid and flavonoid biosynthesis genes are highly expressed in leaf epidermal cells (including the stomatal complex and guard cells); and terpenoid and steroid biosynthesis genes are expressed specifically in leaf mesophyll cells. A number of novel and cell-specific transcription factors involved in secondary metabolite biosynthesis were identified, including MYB17, WRKY12, WRKY31, ERF13, GT_2, and bHLH46. Our research establishes the transcriptional landscape of major cell types in T. mairei leaves at a single-cell resolution and provides valuable resources for studying the basic principles of cell-type-specific regulation of secondary metabolism.
Asunto(s)
Taxus , Taxus/genética , Taxus/química , Taxus/metabolismo , Paclitaxel/metabolismo , Taxoides/metabolismo , Espectrometría de Masas , Hojas de la Planta/genética , Hojas de la Planta/metabolismoRESUMEN
Because of the report on the abnormal local fertility rate at Taizhou area, which is a famous e-waste disassembly center in China, the hormone-like effects in the surface sediment from the local river was investigated. Compared to the control site DG, significant estrogenic effects (p < 0.01) were observed at e-waste recycling sites ranging from 6.01 to 29.31 nmol/kg dw E2 equivalents by water extraction while ranging from 20.47 to 135.02 nmol/kg dw by organic extraction. When coincubated with E2, the water and the organic extractions displayed significant (p < 0.01) synergistic and anti-estrogenic effects respectively.
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Residuos Electrónicos , Disruptores Endocrinos/análisis , Sedimentos Geológicos/química , Contaminantes Químicos del Agua/análisis , China , Disruptores Endocrinos/toxicidad , Monitoreo del Ambiente , Estrógenos/análisis , Bifenilos Policlorados/análisis , Bifenilos Policlorados/toxicidad , Hidrocarburos Policíclicos Aromáticos/análisis , Hidrocarburos Policíclicos Aromáticos/toxicidad , Administración de Residuos , Contaminantes Químicos del Agua/toxicidad , Contaminación Química del Agua/estadística & datos numéricosRESUMEN
The estrogen pollution in the Tiaoxi River, which is the main source river for Taihu Lake, was investigated by chemical and bioassay analysis. Most estrogens species, except estrone, were not detected by the chemical analysis using liquid chromatography coupled with tandem mass spectrometry. The concentration of estrone in the samples ranged from ND (below the detection limit) to 17.25 ng/L. The estrogen activity in most water samples was also determined by the yeast estrogen screen. The 17ß-estradiol equivalent in the intake of Taihu Lake was 17.60 ng/L and was present in all water samples. This study demonstrates that combining chemical and bioassay analysis is an effective way to detect environmental contamination by estrogen species. Furthermore, the results indicate that the risk of estrogen contamination in the Tiaoxi River should not be ignored.
Asunto(s)
Monitoreo del Ambiente/métodos , Estrógenos/análisis , Ríos/química , Contaminantes Químicos del Agua/análisis , Bioensayo , Cromatografía Liquida , Espectrometría de Masas en Tándem , Contaminación Química del Agua/estadística & datos numéricosRESUMEN
The authros developed a new approach to preparing the Au@SiO2 core-shell nanostructure. The morphology and stability of the composite were characterized by the UV-Vis absorption spectroscopy and transmission electron microscopy (TEM) methods. The stable SERS spectra were obtained from the PMBA-functionalized Au@SiO2 composite. In addition, the authros succeeded in adjusting the thickness of SiO2 layer by controlling the precursor consumption. The stability of Au@SiO2 in basic solution was studied and the results showed that the SiO2 shell was facile to be etched. The present work may provide a reference and gist for research on the preparation, storage and application of Au@SiO2.
RESUMEN
E-waste generation has become a serious environmental challenge worldwide. Taizhou of Zhejiang Province, situated on the southeast coastline of China, has been one of the major e-waste dismantling areas in China for the last 40 years. In this review, we focused on the polychlorinated biphenyl (PCB) trends in environmental compartments, burden and impact to humans, food safety, and health risk assessment from Taizhou, China. The review suggested that PCBs showed dynamic trends in air, soil, water, biodiversity, and sediments. Soils and fish samples indicated higher levels of PCBs than sediments, air, water, and food items. PCB levels decreased in soils with the passage of time. Agriculture soils near the e-waste recycling sites showed more levels of total PCBs than industrial soils and urban soils. Dioxin-like PCB levels were higher in humans near Taizhou, suggesting that e-waste pollution could influence humans. Compared with large-scale plants, simple household workshops contributed more pollution of PCBs to the environment. Pollution index, hazard quotient, and daily intake were higher for PCBs, suggesting Taizhou should be given priority to manage the e-waste pollution. The elevated body burden may have health implications for the next generation. The areas with stricter control measures, strengthened laws and regulations, and more environmentally friendly techniques indicated reduced levels of PCBs. For environment protection and health safety, proper e-waste dismantling techniques, environmentally sound management, awareness, and regular monitoring are very important.
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Residuos Electrónicos , Bifenilos Policlorados , Animales , China , Residuos Electrónicos/análisis , Monitoreo del Ambiente , Inocuidad de los Alimentos , Humanos , Bifenilos Policlorados/análisis , Reciclaje , SueloRESUMEN
Taxus trees are major natural sources for the extraction of taxol, an anti-cancer agent used worldwide. Taxus media is a dioecious woody tree with high taxol yield. However, the sexually dimorphic accumulation of taxoids in T. media is largely unknown. Our study revealed high accumulation of taxoids in female T. media trees using a UPLC-MS/MS method. Thereafter, many differential metabolites and genes between female and male T. media trees were identified using metabolomic and transcriptomic analyses, respectively. Most of the taxol-related genes were predominantly expressed in female trees. A female-specific R2R3-MYB transcription factor gene, TmMYB39, was identified. Furthermore, bimolecular fluorescence complementation and yeast two-hybrid assays suggested the potential interaction between TmMYB39 and TmbHLH13. Several taxol biosynthesis-related promoter sequences were isolated and used for the screening of MYB recognition elements. The electrophoretic mobility shift assay indicated that TmMYB39 could bind to the promoters of the GGPPS, T10OH, T13OH, and TBT genes. Interaction between TmMYB39 and TmbHLH13 transactivated the expression of the GGPPS and T10OH genes. TmMYB39 might function in the transcriptional regulation of taxol biosynthesis through an MYB-bHLH module. Our results give a potential explanation for the sexually dimorphic biosynthesis of taxol in T. media.
RESUMEN
Polychlorinated biphenyls (PCBs) are persistent organic pollutants that degrade slowly in the environment. Humic acid (HA), the main component of soil organic matter, or more specifically, the quinone moieties in HA, is generally regarded as an "electron shuttle" between pollutants and microorganisms, which could promote microbial remediation of contamination. In this study, we examined the dechlorination of PCB153 by adding HA and anthraquinone-2,6-disulfonate (AQDS, a model compound of quinones) to systems containing PCB dechlorinators, analyzed the composition and functional gene network of the microbial community by metagenomics, and explored the role of HA by modifying or substituting carbon sources or electron donors. However, this study found that HA accelerated microbial dechlorination of PCBS, while AQDS did not. Moreover, HA without quinone activity still promoted dechlorination, but not without carbon source or electron donor. Metagenomic analysis showed that HA did not promote the growth of PCB dechlorinator (Dehalococcoides), but the transmembrane electron carriers in the HA group were higher than those in the AQDS group and the control group, so HA may have promoted the electron transport process. This study is helpful for microbial remediation of PCB contamination, and provides new insights into the role that HA plays in the biogeochemical cycle.