Stable closed-loop fiber-optic delay of arbitrary radio-frequency waveforms.

Thermal drifts in long fiber-optic delay lines are compensated based on chromatic dispersion. An arbitrary input radio-frequency (RF) waveform and a control RF sine wave modulate two different tunable laser sources and are coupled into the fiber delay line. The RF phase of the control tone at the output of the delay line is monitored and used to adjust the wavelengths of both sources, so that the effects of thermal drifts and dispersion cancel out. The input and control waveforms are separated in the optical domain, and no restrictions are imposed on their RF spectra. A figure of merit is proposed, in terms of the fiber delay, range of temperature changes that may be compensated for, and residual delay variations. An upper bound on performance is established in terms of the specifications of the tunable lasers. The principle is used in the stable distribution of sine waves and of broadband linear frequency-modulated (LFM) waveforms, which are commonly employed in radar systems. Lastly, the method is incorporated in stable interrogation of a localized hot-spot within a high-resolution, distributed Brillouin fiber sensing setup. The results demonstrate the applicability of the proposed protocol in the processing of arbitrary waveforms, as part of larger, more complex systems.

Large one-time photo-induced tuning of directional couplers in chalcogenide-on-silicon platform.

The stable one-time tuning of silicon-photonic directional couplers, over a broad range of coupling ratios, is achieved through the selective photo-removal of an upper cladding layer of chalcogenide glass. Analysis shows that the coupling coefficient per unit length between two parallel fully-etched silicon waveguides may be changed by 45%. The power coupling ratio of a 50 µm-long directional coupler between two such waveguides may be tuned arbitrarily between 0 and 1, with weak residual wavelength dependence. Smaller modifications in the coupling coefficient per unit length are obtained between two partially-etched ridge waveguides, on the order of 10%. The proposed procedure is demonstrated in the post-fabrication tuning of transmission notches of a race-track resonator, from over-coupling through critical coupling to weak coupling. The extinction ratio of specific resonances is varied between 4 and 40 dB. The coupling ratio of a tuned device remains stable following three months of storage.

Large photo-induced index variations in chalcogenide-on-silicon waveguides.

The postfabrication modification of the group delay in silicon-photonic waveguides is proposed, simulated and demonstrated experimentally. Group delay variations of 2% are achieved through photo-induced changes to an upper cladding layer of photosensitive As10Se90 chalcogenide glass. The illumination of the cladding layer by intense green light for a few seconds leads to mass transfer and removal of material, away from irradiated regions. The phenomenon is employed in the localized removal of the cladding layer from above the core region of a silicon-on-insulator waveguide, thereby modifying its phase and group delays. Using the proposed method, the free spectral range of a chalcogenide-on-silicon Mach-Zehnder interferometer was modified by 1%. The technique is applicable to the postfabrication adjustment of the frequency response of silicon-photonic filters, comprised of several cascaded elements.

Localized and stationary dynamic gratings via stimulated Brillouin scattering with phase modulated pumps.

A novel technique for the localization of stimulated Brillouin scattering (SBS) interaction is proposed, analyzed and demonstrated experimentally. The method relies on the phase modulation of two counter-propagating optical waves by a common pseudo-random bit sequence (PRBS), these waves being spectrally detuned by the Brillouin frequency shift. The PRBS symbol duration is much shorter than the acoustic lifetime. The interference between the two modulated waves gives rise to an acoustic grating that is confined to narrow correlation peaks, as short as 1.7 cm. The separation between neighboring peaks, which is governed by the PRBS length, can be made arbitrarily long. The method is demonstrated in the generation and applications of dynamic gratings in polarization maintaining (PM) fibers. Localized and stationary acoustic gratings are induced by two phase modulated pumps that are polarized along one principal axis of the PM fiber, and interrogated by a third, readout wave which is polarized along the orthogonal axis. Using the proposed technique, we demonstrate the variable delay of 1 ns-long readout pulses by as much as 770 ns. Noise due to reflections from residual off-peak gratings and its implications on the potential variable delay of optical communication data are discussed. The method is equally applicable to the modulation of pump and probe waves in SBS over standard fibers.

Tunable and reconfigurable multi-tap microwave photonic filter based on dynamic Brillouin gratings in fibers.

We propose and experimentally demonstrate new architectures to realize multi-tap microwave photonic filters, based on the generation of a single or multiple dynamic Brillouin gratings in polarization maintaining fibers. The spectral range and selectivity of the proposed periodic filters is extensively tunable, simply by reconfiguring the positions and the number of dynamic gratings along the fiber respectively. In this paper, we present a complete analysis of three different configurations comprising a microwave photonic filter implementation: a simple notch-type Mach-Zehnder approach with a single movable dynamic grating, a multi-tap performance based on multiple dynamic gratings and finally a stationary grating configuration based on the phase modulation of two counter-propagating optical waves by a common pseudo-random bit sequence (PRBS).

A unique ATP-dependent DNA topoisomerase from trypanosomatids.

Crithidia fasciculata DNA topoisomerase (22) has been purified to near homogeneity from trypanosomatid cell extracts. The purified enzyme catalyzes the reversible interconversion of monomeric duplex DNA circles and catenanes in an ATP dependent reaction. Reversible catenane formation is affected by the ionic strength and is dependent upon the action of a crithidial DNA binding protein, which could be substituted for the polyamine spermidine. Covalently sealed DNA circles are specifically used as substrates for decatenation. Nicking, but not relaxation per se, inhibits network decatenation and has little or no effect on catenane formation. The catalytic properties of this enzyme and its potential role in the prereplication release and post replication reattachment of kDNA minicircles are discussed.

Reversible decatenation of kinetoplast DNA by a DNA topoisomerase from trypanosomatids.

DNA topoisomerase activity detected in cell extracts of the trypanosomatid Crithidia fasciculata interlocks kinetoplast DNA duplex minicircles into huge catenane forms resembling the natural kinetoplast DNA networks found in trypanosomes. Catenation of duplex DNA circles is reversible and equilibrium is affected by ionic strength, and by spermidine. The reaction requires magnesium, is ATP dependent and is inhibited by high concentrations of novobiocin. Extensive homology between duplex DNA rings was not required for catenane formation since DNA circles with unrelated sequences could be interlocked into mixed network forms. Covalently sealed catenaned DNA circles are specifically used as substrates for decatenation. No such preference for covalently sealed duplex DNA rings was observed for catenate formation. Its catalytic properties and DNA substrate preference, suggest a potential role for this eukaryotic topoisomerase activity in the replication of kinetoplast DNA.

Kinetoplast DNA minicircles of trypanosomatids encode for a protein product.

The major constituent of the trypanosomal kinetoplast DNA network are several thousand duplex DNA minicircles whose biological function is still unknown. The coding capacity and expression of these DNA minicircles, was studied in the trypanosomatid Crithidia fasciculata. Kinetoplast DNA minicircle fragments inserted into bacterial plasmid vectors were expressed in the bacterial cell. Sera elicited in rabbits, by immunization with the translational products of kinetoplast DNA minicircles in E. coli, reacted specifically with Crithidia fasciculata cellular antigens. It is inferred that kinetoplast DNA minicircles contain long open reading frames of nucleotides which are expressed in the trypanosomatid cell.