Effects of humic acid on nutrient removal efficiency of aquatic duckweed (Lemna minor) and both growth performance, and hemato-biochemical parameters of Nile tilapia (Oreochromis niloticus) cultured in water recirculating system.

This study was carried out to evaluate the effects of humic acid (HA) on the nutrient removal efficiencies of aquatic duckweed plant (Lemna minor) from a water recirculating system used to culture Nile tilapia (Oreochromis niloticus) fish for 30 days. The HA was added to water at three concentrations of 0 (Control), 1.5, and 3 mg/L in triplicate. Water quality parameters, growth performance, and some hemato-biochemical parameters of the fish in variable HA concentrations were compared. The total ammonia nitrogen (TAN) and total phosphorous (TP) removal efficiency of L. minor increased with increasing the HA concentration from 0 mg/L to 3 mg/L (p < 0.05). The concentration of nitrate (NO3-) in the HA-3 mg/L was higher than that in the other groups on days 20 and 30 of the fish cultivation period (p < 0.05). The growth performance of fish improved in the HA-3 mg/L compared to the other groups. The addition of different concentrations of HA to water had no adverse effect on the hematological properties of the Nile tilapia. The plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) levels in the HA-0 mg/L and HA-1.5 mg/L groups were higher than in the HA-3 mg/L (p < 0.05). No significant differences in the plasma glucose and cholesterol levels were observed between the HA-groups (p > 0.05), while the triglyceride level increased in the HA-3 mg/L compared to the control (p < 0.05). These results indicated that adding HA to water could be an effective method to enhance the bioremediation performance of the aquatic duckweed plants as biofilter and thus improve water quality, subsequently, fish growth performance in RASs.

The current study applied aquatic duckweed plant (Lemna minor) as a new biofilter in a water recirculating system used to culture Nile tilapia (Oreochromis niloticus) fish. The effects of three concentrations of humic acid (HA) as water additive on the nutrient removal efficiency of L. minor from water were investigated. HA improved bioremediation performance of the aquatic duckweed plant.

Systematic Review of Studies Examining Transtibial Prosthetic Socket Pressures with Changes in Device Alignment.

Suitable lower-limb prosthetic sockets must provide an adequate distribution of the pressures created from standing and ambulation. A systematic search for articles reporting socket pressure changes in response to device alignment perturbation was carried out, identifying 11 studies. These were then evaluated using the American Academy of Orthotists and Prosthetists guidelines for a state-of-the-science review. Each study used a design where participants acted as their own controls. Results were available for 52 individuals and five forms of alignment perturbation. Four studies were rated as having moderate internal and external validity, the remainder were considered to have low validity. Significant limitations in study design, reporting quality and in representation of results and the suitability of calculations of statistical significance were evident across articles. Despite the high inhomogeneity of study designs, moderate evidence supports repeatable changes in pressure distribution for specific induced changes in component alignment. However, there also appears to be a significant individual component to alignment responses. Future studies should aim to include greater detail in the presentation of results to better support later meta-analyses.

Mechanical testing of transtibial prosthetic sockets: A discussion paper from the American Orthotic and Prosthetic Association Socket Guidance Workgroup.

BACKGROUND: The advent of novel manufacturing technologies, materials, and socket design concepts could introduce risks to prosthetic limb users, as the existing knowledge base for safe fabrication may not apply. Moreover, although structural test standards exist for mass-produced prosthetic components, they are not applicable to prosthetic sockets. METHODS: The "AOPA Socket Guidance Workgroup" was formed in 2020 to provide the prosthetic community with evidence-based clinical best practices and methods in the field of prosthetic socket structural analysis. This multidisciplinary expert workgroup undertook a critical analysis of the knowledge gaps regarding the requirements for mechanical testing of lower limb prosthetic sockets. RESULTS: The Workgroup identified knowledge gaps in 4 domains. Domain 1 describes the shape and composition of a mock residual limb, required to support and generate in vivo representative loading within the socket. Domain 2 concerns prosthetic socket coordinate systems and alignment. Domain 3 regards the components and requirements of test specimens. Finally, Domain 4 considers test conditions, loading parameters, and acceptance criteria. CONCLUSIONS: This paper describes these knowledge gaps in detail and recommends potential solution approaches based on literature review, group consensus around existing knowledge, or the formation of new study groups to fill each knowledge gap. Our intent is for the recommendations arising from this paper to support the community (e.g., researchers in the clinic, academia, industry, and funders) in addressing these knowledge gaps.

Integrated multitrophic aquaculture (IMTA) as an environmentally friendly system for sustainable aquaculture: functionality, species, and application of biofloc technology (BFT).

Aquaculture is one of the fastest-growing industries in the world, and its prominent role has been proven in supplying food for the growing world population. The expected growth of aquaculture requires the development of responsible and sustainable approaches, technologies, culture systems, and practices. The integrated multitrophic aquaculture (IMTA) system has been developed over the past decades. This system is based on the use of all food levels for simultaneous production of some aquaculturally species in a way that contributes to environmental sustainability (biocontrol), economic stability (product diversity and risk reduction), and social acceptance (better management operations). In IMTA, selecting suitable culture species and considering their appropriate population size is absolutely necessary to achieve an optimal biological and chemical process, improving the ecosystem health and sustainability of the industry. Biofloc technology (BFT) is closely related to the IMTA system, where the IMTA potential can be used to control suspended solids in aquaculture systems with limited water exchange. This study reviews the significance of IMTA systems, potential target species for cultivation, the relationship between BFT and IMTA, total suspended solids control, the economics of IMTA farming, and the recent findings in these fields.

Low-temperature argon plasma jet with cascading electrode technique for biological applications.

In this study, the design, performance, and characteristics of a low-temperature argon plasma jet with cascading electrode technique (APJCE) are presented. APJCE is designed based on a tip-ring structure with a cascading ring. The effect of plasma jet driven by repetitive high-voltage microsecond pulses in APJCE structure was measured qualitatively in local surface temperature detection system. Then, by applying the generated plasma jet to biological surface and measuring and characterizing the electrical parameters, we obtained a plasma jet, which is electrically and thermally in the cold plasma regime. Simulation of the electric field distribution in the nozzle also yielded similar results to the experimental results. Finally, by cascading electrodes, we guided the plasma column to the nozzle output so that the plasma temperature within four centimeters of the nozzle output is 37 °C. The resulting plasma jets were studied by atomic emission spectroscopy and the intensity of the spectral lines of the atmospheric argon plasma jet spectra was obtained as a final experimental result at the output.

Analysis of lower limb prosthetic socket interface based on stress and motion measurements.

The study was designed to establish a biomechanical assessment platform for the lower limb residuum/socket interface as a function of duration and speed of movement. The approach exploits an interface sensor which measures multi-directional stresses at the interface. The corresponding interface coupling motion was assessed using a 3D motion capture system. A longitudinal study, involving a trans-femoral amputee, was conducted with nine repeated level walking sessions over a 12-month period. The effect of walking speed on interface biomechanics was also assessed. Interface peak pressures and shear stresses in the range of 55-59 kPa and 12-19 kPa were measured, respectively, over all sessions in the 12 months study period at the posterior-proximal location of the residuum. The peak pressure and longitudinal shear values were found to fluctuate approximately 11% and 40% as against its maximum value, respectively, over 12 months. In addition, up to 12° of angular coupling and up to 28 mm of pistoning were recorded over a gait cycle, which was found to change by 29% and 45% respectively over the study period. The variation in walking speed, by altering self-selected cadence, resulted in changes of pressure and shear stresses at mid-stance of the gait cycle. In particular, as compared with self-selected cadence, for fast speed, peak pressure and peak longitudinal shear stress decreased by 5% and 33%, respectively. For slow speed, peak pressure and peak longitudinal shear stress increased by 7% and 17%, respectively. The corresponding angular and pistoning revealed a variation of up to 29% and 45%, respectively. This biomechanical assessment approach shows promise in the quantitative assessment of interface kinematics and kinetics for lower limb prosthetics, the usage of which could assist the clinical assessment of prosthetic socket fit.

Can microprocessor knees reduce the disparity in trips and falls risks between above and below knee prosthesis users?

While all lower limb prosthesis walkers have a high risk of tripping and/or falling, above knee prosthesis users are reported to fall more frequently. Recognising this, engineers designed microprocessor knees (MPK) to help mitigate these risks, but to what extent these devices reduce this disparity between above and below knee users is unclear. A service review was carried out in a prosthetic limb centre regarding the frequency of trips and falls in the previous four weeks. Data from unilateral, community ambulators were extracted. Ordered logistic regressions were applied to investigate whether MPKs mitigated the increased risk of trips and falls for prosthetic knee users, compared to below knee prosthesis users. Socio-demographics (sex, age), prosthesis (prosthesis type, years of use), health (comorbidities, vision, contralateral limb status, medication), and physical function (use of additional walking aids, activity level) were included as covariates. Of the 315 participants in the analysis, 57.5% reported tripping and 20.3% reported falling. Non-microprocessor prosthetic knee (non-MPK) users were shown to trip significantly more than below knee prosthesis users (OR = 1.96, 95% CI = 1.17-3.28). Other covariates showing a significant association included contralateral limb injuries (OR = 1.91, 95% CI = 1.15-3.18) and using an additional walking aid (OR = 1.99, 95% CI = 1.13-3.50). Non-MPK users were also shown to fall significantly more than below knee prosthesis users (OR = 3.34, 95% CI = 1.73-6.45), with no other covariates showing a significant association. MPK users did not show an increased frequency of trips (OR = 0.74, 95% CI = 0.33-1.64) or falls (OR = 0.34, 95% CI = 0.18-2.62), compared to below knee prosthesis users. Of those who tripped at least once in the previous four weeks, those using a non-MPK (OR = 2.73, 95% CI = 1.30-5.74) presented an increased frequency of falling. These findings provide evidence to suggest that the use of MPKs reduces the difference in falls risk between above knee and below knee prosthesis users, providing justification for their provision.

A Prosthetic Shank With Adaptable Torsion Stiffness and Foot Alignment.

Torsion adapters in lower limb prostheses aim to increase comfort, mobility and health of users by allowing rotation in the transversal plane. A preliminary study with two transtibial amputees indicated correlations between torsional stiffness and foot alignment to increase comfort and stability of the user depending on the gait situation and velocity. This paper presents the design and proof-of-concept of an active, bio-inspired prosthetic shank adapter and a novel approach to create a user-specific human-machine interaction through adapting the device's properties. To provide adequate support, load data and subjective feedback of subjects are recorded and analyzed regarding defined gait situations. The results are merged to an user individual preference-setting matrix to select optimal parameters for each gait situation and velocity. A control strategy is implemented to render the specified desired torsional stiffness and transversal foot alignment values to achieve situation-dependent adaptation based on the input of designed gait detection algorithms. The proposed parallel elastic drive train mimics the functions of bones and muscles in the human shank. It is designed to provide the desired physical human-machine interaction properties along with optimized actuator energy consumption. Following test bench verification, trials with five participants with lower limb amputation at different levels are performed for basic validation. The results suggest improved movement support in turning maneuvers. Subjective user feedback confirmed a noticeable reduction of load at the stump and improved ease of turning.

The influence of a microprocessor-controlled hydraulic ankle on the kinetic symmetry of trans-tibial amputees during ramp walking: A case series.

INTRODUCTION: Asymmetrical limb loading is believed to cause health problems for lower limb amputees and is exacerbated when walking on slopes. Hydraulically damped ankle-feet improve ground compliance on slopes compared to conventional prosthetic feet. Microprocessor-controlled hydraulic ankle-feet provide further adaptation by dynamically adjusting viscoelastic damping properties. METHOD: Using a case series design, gait analysis was performed with four trans-tibial amputees. There were two walking conditions (ramp ascent and descent) and two prosthetic foot conditions (microprocessor-control on and off - MPF-on and MPF-off). Total support moment integral ( M I sup ) and degree-of-asymmetry were compared across foot conditions. RESULTS: During ramp descent, the transition of prosthetic ankle moment from dorsiflexion to plantarflexion occurred earlier in stance phase with MPF-on, slowing the angular velocity of the shank. During ramp ascent, the MPF-on dorsiflexion/plantarflexion moment transition occurred later, meaning less resistance to shank rotation in early stance and increasing walking speed by up to 6%. For both slope conditions, sound limb M I sup was universally decreased with MPF-on (4-13% descent, 3-11% ascent). DISCUSSION: Microprocessor-control of hydraulic ankle-feet reduced the total loading of the sound limb joints, for both walking conditions, for all participants. This may have beneficial consequences for long-term joint health and walking efficiency.

Microprocessor knees with 'standing support' and articulating, hydraulic ankles improve balance control and inter-limb loading during quiet standing.

INTRODUCTION: Trans-femoral amputees are at risk of musculoskeletal problems that are in part caused by loading asymmetry during activities, such as prolonged standing, particularly on uneven or sloped ground. METHODS: Four prosthetic conditions were tested; microprocessor knee 'standing support' mode activated (ON) and deactivated (OFF), combined with a rigidly attached foot (RA) and with an articulating, hydraulic ankle-foot (HA). Five trans-femoral amputees and five able-bodied controls were measured using a motion capture system and a force plate while standing, facing down a 5° slope. Ground reaction force distributions and centre-of-pressure root-mean-square (COP RMS) were calculated as outcome measures. RESULTS: Compensatory kinematic adjustments were observed for RA conditions but not for HA conditions. HA-OFF reduced ground reaction force degree-of-asymmetry for all five amputees, compared to RA-OFF. RA-ON reduced ground reaction force degree-of-asymmetry for four amputees, compared to RA-OFF. In terms of balance, the HA conditions reduced the mean inter-limb COP RMS by 24-25% compared to equivalent RA conditions, while ON conditions reduced it by 9-11%, compared to equivalent OFF conditions. CONCLUSIONS: It is important to consider both prosthetic knee and ankle technologies when prescribing devices to trans-femoral amputees. The combination of hydraulic ankle and knee standing support technologies produced outcomes closest to normal biomechanics.

Rethinking the foam cosmesis for people with lower limb absence.

BACKGROUND AND AIM: A recent survey of people with lower limb absence revealed that patients' satisfaction with their foam cosmesis is lower than desired. The aim of this project was to improve the lifelike appearance, functionality and durability of the cosmesis through a user-centred design methodology. TECHNIQUE: Concept development and prototyping led to a new cosmesis design which features a cut-out located at the knee, inserted with an artificial patella made of a more rigid foam. It also features a full-length zip which provides easy access for maintenance. The new cosmesis was then mechanically tested for over 1 million cycles and clinically tested by six transfemoral prosthesis users over 18 patient months. DISCUSSION: The new design is significantly more durable than the current standard model and has an enhanced lifelike appearance. It has potential to improve users' body image and reduce costs for healthcare providers. Clinical relevance This study contributes to practice by offering a new cosmesis design with enhanced appearance and durability, with the potential to improve patients' body image and reduce costs associated with cosmesis fitting and maintenance.

Performance of Optimized Prosthetic Ankle Designs That Are Based on a Hydraulic Variable Displacement Actuator (VDA).

Current energy storage and return prosthetic feet only marginally reduce the cost of amputee locomotion compared with basic solid ankle cushioned heel feet, possibly due to their lack of push-off at the end of stance. To the best of our knowledge, a prosthetic ankle that utilizes a hydraulic variable displacement actuator (VDA) to improve push-off performance has not previously been proposed. Therefore, here we report a design optimization and simulation feasibility study for a VDA-based prosthetic ankle. The proposed device stores the eccentric ankle work done from heel strike to maximum dorsiflexion in a hydraulic accumulator and then returns the stored energy to power push-off. Optimization was used to establish the best spring characteristic and gear ratio between ankle and VDA. The corresponding simulations show that, in level walking, normal push-off is achieved and, per gait cycle, the energy stored in the accumulator increases by 22% of the requirements for normal push-off. Although the results are promising, there are many unanswered questions and, for this approach to be a success, a new miniature, low-losses, and lightweight VDA would be required that is half the size of the smallest commercially available device.

Development of a residuum/socket interface simulator for lower limb prosthetics.

Mechanical coupling at the interface between lower limb residua and prosthetic sockets plays an important role in assessing socket fitting and tissue health. However, most research lab-based lower limb prosthetic simulators to-date have implemented a rigid socket coupling. This study describes the fabrication and implementation of a lower limb residuum/socket interface simulator, designed to reproduce the forces and moments present during the key loading phases of amputee walking. An artificial residuum made with model bones encased in silicone was used, mimicking the compliant mechanical loading of a real residuum/socket interface. A 6-degree-of-freedom load cell measured the overall kinetics, having previously been incorporated into an amputee's prosthesis to collect reference data. The developed simulator was compared to a setup where a rigid pylon replaced the artificial residuum. A maximum uniaxial load of 850 N was applied, comparable to the peak vertical ground reaction force component during amputee walking. Load cell outputs from both pylon and residuum setups were compared. During weight acceptance, when including the artificial residuum, compression decreased by 10%, while during push off, sagittal bending and anterior-posterior shear showed a 25% increase and 34% decrease, respectively. Such notable difference by including a compliant residuum further highlighted the need for such an interface simulator. Subsequently, the simulator was adjusted to produce key load cell outputs briefly aligning with those from amputee walking. Force sensing resistors were deployed at load bearing anatomic locations on the residuum/socket interface to measure pressures and were compared to those cited in the literature for similar locations. The development of such a novel simulator provides an objective adjunct, using commonly available mechanical test machines. It could potentially be used to provide further insight into socket design, fit and the complex load transfer mechanics at the residuum/socket interface, as well as to evaluate the structural performance of prostheses.

A combined kinematic and kinetic analysis at the residuum/socket interface of a knee-disarticulation amputee.

The bespoke interface between a lower limb residuum and a prosthetic socket is critical for an amputee's comfort and overall rehabilitation outcomes. Analysis of interface kinematics and kinetics is important to gain full understanding of the interface biomechanics, which could aid clinical socket fit, rehabilitation and amputee care. This pilot study aims to investigate the dynamic correlation between kinematic movement and kinetic stresses at the interface during walking tests on different terrains. One male, knee disarticulation amputee participated in the study. He was asked to walk on both a level surface and a 5° ramped surface. The movement between the residuum and the socket was evaluated by the angular and axial couplings, based on the outputs from a 3D motion capture system. The corresponding kinetic stresses at anterior-proximal (AP), posterior-proximal (PP) and anterior-distal (AD) locations of the residuum were measured, using individual stress sensors. Approximately 8° of angular coupling and up to 32 mm of axial coupling were measured when walking on different terrains. The direction of the angular coupling shows strong correlation with the pressure difference between the PP and AP sensors. Higher pressure was obtained at the PP location than the AP location during stance phase, associated with the direction of the angular coupling. A strong correlation between axial coupling length, L, and longitudinal shear was also evident at the PP and AD locations i.e. the shortening of L corresponds to the increase of shear in the proximal direction. Although different terrains did not affect these correlations in principle, interface kinematic and kinetic values suggested that gait changes can induce modifications to the interface biomechanics. It is envisaged that the reported techniques could be potentially used to provide combined kinematics and kinetics for the understanding of biomechanics at the residuum/socket interface, which may play an important role in the clinical assessment of prosthetic component settings, including socket fit quality.

Characterisation of dynamic couplings at lower limb residuum/socket interface using 3D motion capture.

Design and fitting of artificial limbs to lower limb amputees are largely based on the subjective judgement of the prosthetist. Understanding the science of three-dimensional (3D) dynamic coupling at the residuum/socket interface could potentially aid the design and fitting of the socket. A new method has been developed to characterise the 3D dynamic coupling at the residuum/socket interface using 3D motion capture based on a single case study of a trans-femoral amputee. The new model incorporated a Virtual Residuum Segment (VRS) and a Socket Segment (SS) which combined to form the residuum/socket interface. Angular and axial couplings between the two segments were subsequently determined. Results indicated a non-rigid angular coupling in excess of 10° in the quasi-sagittal plane and an axial coupling of between 21 and 35 mm. The corresponding angular couplings of less than 4° and 2° were estimated in the quasi-coronal and quasi-transverse plane, respectively. We propose that the combined experimental and analytical approach adopted in this case study could aid the iterative socket fitting process and could potentially lead to a new socket design.

Biochemical responses of juvenile European sturgeon, (Huso huso) to a sub-lethal level of copper and cadmium in freshwater and brackish water environments.

In Caspian Sea basin, sturgeons spend the larval and juvenile stages in freshwaters of rivers and then, they migrate to brackish waters of the sea where they grow and mature. With regard to the elevation of the metal concentrations in coastal waters and sediments of the Caspian Sea and its adjacent rivers, it is likely that juvenile sturgeon are exposed to sub-lethal levels of metals during seawater entry process. We compared the biochemical responses of juvenile European sturgeon, (Beluga, Huso huso) exposed to a sub-lethal level of copper (Cu, 20 µg/L) and cadmium (Cd, 300 µg/L) in freshwater (FW, 0 ppt) and brackish water (BW, 11 ppt) for seven days. The results showed that the levels of plasma glucose increased significantly in BW and in all metal exposed groups. Also, plasma cortisol concentrations showed significant increases when juveniles were exposed to BW, Cu(FW/BW) and Cd(BW). The activity of liver superoxide dismutase (SOD) decreased significantly in BW compared with FW. Moreover, Cu and Cd exposure enhanced the activity of SOD in BW, while SOD did not show any changes in FW. The levels of tissue and plasma proteins as well as plasma triiodothyronine (T3), thyroxine (T4) and liver Catalase (CAT) activity remained constant when animals were exposed to Cu/Cd in both FW and BW environments. Our data indicate that exposure of juvenile beluga to BW stimulated the general biochemical responses of stress such as cortisol and glucose, while sub-lethal exposure to Cu and Cd caused oxidative stress in BW environment but not in FW.

Effects of mass and momentum of inertia alternation on individual muscle forces during swing phase of transtibial amputee gait.

A computer simulation was carried out to investigate the forces of lower extremity muscles in the swing phase of a transtibial amputee gait. With each muscle as an ideal force generator, the lower extremity was simulated as a two-degrees of freedom linkage with the hip and knee as its joints. Kinematic data of hip and knee joints were recorded by a motion analysis system. Through a static optimization approach, the forces exerted by muscles were determined so that recorded hip and knee joint angles were produced. Simulation results showed that when the mass of prosthetic foot is increased, muscle forces increase, too. This result is in accord with experimental and theoretical studies that reported an increase in leg mass lead to higher electromyography activity of muscles, and energetic of walking. However, since prosthetic foot moment of inertia is smaller than that of thigh and prosthetic shank, its alternation does not have noticeable effect on muscle forces.