Convergent Synthesis of the Nonreducing Hexasaccharide Fragment and the Dodecasaccharide Scaffold of Marine Lipopolysaccharide Axinelloside A.

A convergent synthesis of the dodecasaccharide scaffold of axinelloside A was achieved through Au(I)-catalyzed [6+6] glycosylation. The initially devised [3+1+2] assembly of the nonreducing hexasaccharide fragment was low-yielding, whereas a convergent [3+3] glycosylation under Au(I) catalysis was proven feasible, allowing for a semi-gram scale preparation of the wanted hexasaccharide. The requisite 1,2-cis glycosidic bonds were forged in a highly stereoselective fashion by virtue of remote acetyl group participation, and judicious manipulation of protecting groups. The synthetic dodecasaccharide has been properly protected for the downstream elaboration toward its natural form.

Synthesis of the Reducing-end Hexasaccharide Fragment of Marine Lipopolysaccharide Axinelloside A.

Chemical synthesis of an orthogonally protected hexasaccharide relevant to the reducing-end half of axinelloside A, a highly sulfated marine lipopolysaccharide, is disclosed. The synthesis features preparation of the scyllo-inositol unit via a Ferrier-type-II rearrangement, construction of the 1,2-cis-glycosidic bonds via remote participation, and concise [2+2+2] assembly via Au(I)-catalyzed glycosylation.

Immunomodulatory effect of Myrtenol on benzo (a) pyrene-induced lung cancer in Swiss albino mice via modulation of tumor markers, cytokines and inhibition of PCNA expression.

Lung cancer is one of the most common cancers in men. Although many diagnostic and treatment regimens have been followed in the treatment for lung cancer, increasing mortality rate due to lung cancer is depressing and hence requires alternative plant based therapeutics with with less side-effects. Myrtenol exhibits anti-inflammatory and antioxidant properties. Hence we intended to study the effect of Myrtenol on B(a)P-induced lung cancer. Our study showed that B(a)P lowered hematological count, decreased phagocyte and avidity indices, nitroblue tetrazolium (NBT) reduction, levels of immunoglubulins, antioxidant levels, whereas Myrtenol treatment restored them back to normal levels. On the other hand, xenobiotic and liver dysfunction marker enzymes and pro-inflammatory cytokines were elevated on B(a)P exposure, which retuned back to normal by Myrtenol. This study thus describes the immunomodulatory and antioxidant effects of Myrtenol on B[a]P-induced immune destruction.

Medial Protrusio Technique Versus Structural Autologous Bone-Grafting Technique in Total Hip Arthroplasty for Crowe Type II to III Hip Dysplasia.

BACKGROUND: It is unclear whether acetabular reconstruction techniques have any impact on clinical outcomes. This study aimed to determine (1) whether acetabular reconstruction techniques influenced the position of the acetabular cup and (2) whether clinical outcomes based on the acetabular reconstruction techniques differ in patients undergoing total hip arthroplasty (THA) with Crowe II to III developmental dysplasia of the hip. METHODS: This was a retrospective analysis of prospectively collected data from 69 patients (74 hips) who were treated with cementless THA using medial protrusio technique (MPT) or structural autologous bone-grafting technique (SABT). There were 39 patients (41 hips) included in the MPT group and 30 patients (33 hips) in the SABT group. Clinical and radiographic outcomes were evaluated. RESULTS: All patients were followed up for at least 3 years. There were similar results between the 2 groups in terms of blood loss, Harris hip score, leg length discrepancy, cup inclination, cup anteversion, and proportion of cup coverage (P > .05). The operative time was significantly longer in the SABT group compared with the MPT group (P < .001). The postoperative vertical center of rotation was significantly higher in the MPT group compared with the SABT group (P = .001), and postoperative horizontal center of rotation was significantly shallower in the SABT group compared with the MPT group (P < .001). CONCLUSION: The MPT and SABT provide similar clinical and radiographic outcomes in the management of Crowe II to III developmental dysplasia of the hip by cementless THA. However, the MPT has the advantage of a shorter operative time, whereas the SABT is more conducive to placing the acetabular cup in an anatomic position. LEVEL OF EVIDENCE: Level III, Therapeutic, Case-Control Study.

[Circular RNA-Encoded Proteins in Gastrointestinal Cancer:A Review].

Circular RNAs(CircRNAs)are a class of non-coding RNAs with a covalently closed-loop structure,high stability,and tissue specificity,with the production mechanisms different from linear RNAs.Recent studies have discovered that some CircRNAs can encode proteins via cap-independent translation mechanisms such as internal ribosome entry site,N6-methyladenosine,and rolling loop translation.The encoded proteins regulate homologous linear proteins or downstream signaling pathways via protein bait or other mechanisms,thereby exerting biological functions.Studies have shown that CircRNAs play a role in various diseases,especially in tumor progression,proliferation,invasion,and metastasis and immune regulation.Therefore,by elucidating the expression and roles of proteins encoded by CircRNAs in tumorigenesis and development,this paper is expected to provide new tumor markers and potential targets for tumor diagnosis and treatment.

Photon blockade in the Jaynes-Cummings model with two-photon dissipation.

We propose a scheme to generate a single-photon source based on photon blockade in the Jaynes-Cummings (J-C) model with a two-photon dissipation (TPD) process. We present the optimal conditions for conventional/unconventional photon blockade via the wave function method with an effective Hamiltonian involving TPD. The results show that the second-order correlation function for the J-C model with TPD is considerably less than that of the J-C model with single-photon dissipation. Additionally, the average photon number can reach 0.5 in the large atomic detuning regime. This feature makes the J-C model with TPD a high-quality single photon source.

Risk factors associated with the prevalence of neck and shoulder pain among high school students: a cross-sectional survey in China.

BACKGROUND: After the COVID-19 outbreak, many Chinese high school students have increased their dependence on electronic devices for studying and life, which may affect the incidence of neck and shoulder pain (NSP) in Chinese adolescents. METHODS: To evaluate the prevalence of NSP in high school students and its associated risk factors during COVID-19, a survey was conducted among 5,046 high school students in Shanghai, Qinghai, Henan and Macao during the second semester and summer vacation of the 2019-2020 academic year. The questionnaire included questions regarding demographic characteristics, the prevalence of NSP and lifestyle factors such as sedentary behavior, poor posture and electronic device usage. Univariable and multivariable logistic regression was used to analyze the possible influencing factors for neck and shoulder pain. RESULTS: A total of 4793 valid questionnaires (95.0%) were collected. The results indicated that the prevalence of NSP was 23.7% among high school students. Binary logistic regression analysis revealed that female gender (P < 0.05, OR = 1.82), grade (P < 0.05, range OR 1.40-1.51) and subject selection (P < 0.05, range OR 0.49-0.68) were risk factors for NSP in high school students. Sedentary behavior (P < 0.05, range OR 1.74-2.36), poor posture (P < 0.05, range OR 1.19-2.56), backpack weight (P < 0.05, range OR 1.17-1.88), exercise style and frequency (P < 0.05, range OR 1.18-1.31; P < 0.05, range OR 0.76-0.79, respectively), and the time spent using electronic devices (P < 0.05, range OR 1.23-1.38)had a significant correlation with NSP in high school students. CONCLUSIONS: NSP is currently very common among high school students during the outbreak of COVID-19. Sedentary behavior, poor posture and other factors have a great impact on the occurrence of NSP in high school students. Education regarding healthy lifestyle choices should be advocated for to decrease NSP among high school students, such as more physical activity, changing poor postures and reducing the amount of time spent using electronic devices.

HDAC3 Contributes to Ischemic Stroke by Regulating Interferon Pathway.

BACKGROUND: The inflammation and immune response contribute to ischemic stroke pathology. Damaged brain cells release inflammatory substances to activate the immune system in the acute phase of stroke, including altering the interferon signaling pathway. However, the involvement of histone deacetylation in stroke remains unclear. METHODS: To investigate whether histone deacetylation modulation could regulate the interferon signaling pathway and mediate the pathogenic changes after stroke, the middle cerebral artery occlusion (MCAO) mouse model was treated with histone deacetylase 3 (HDAC3) inhibitor and RGFP966. Additionally, a series of approaches, including middle cerebral artery occlusion (MCAO), real-time polymerase chain reaction (PCR), western blot, 2,3,5-triphenyltetrazolium chloride (TTC) staining, behavioral experiments, and confocal imaging were utilized. RESULTS: It is observed that RGFP966 pretreatment could lead to better outcomes in the MCAO mouse model, including the decrease of infarction volumes, the amelioration of post-stroke anxiety-like behavior, and the relief of inflammatory responses. Furthermore, we found that RGFP966 could counteract the hyperactivation of the interferon signaling pathway and the excessive expression of Z-DNA Binding Protein 1 (ZBP1) in microglia. CONCLUSIONS: We demonstrated a novel mechanism that HDAC3 inhibition could ameliorate the pathological injury after ischemic stroke by downregulating the ZBP1/phosphorylated Interferon Regulatory Factor 3 (p-IRF3) pathway. Thus, these data provide a new promising target for therapies for ischemic stroke.

Impact of salinity on anaerobic ceramic membrane bioreactor for textile wastewater treatment: Process performance, membrane fouling and machine learning models.

Anaerobic membrane bioreactor (AnMBR) shows great potential for textile wastewater treatment, but high salinity in the influent may undermine its performance. This study evaluated the impact of salinity on the treatment performance of an upflow anaerobic sludge blanket (UASB) configured AnMBR using a flat sheet ceramic membrane. The salinity was stepwise increased (0, 5, 10 and 20 g/L) in four phases of the AnMBR operation. Results indicated that increased salinity jeopardized the COD removal efficiency of AnMBR from 92% to 73%, but had a marginal effect on dye removal efficacy (90-96%). Low salinity (5 g/L) boosted the biogas production whilst high salinity (>10 g/L) had a negative impact. Additionally, the increase of salinity resulted in the soluble microbial production (SMP) concentration soar and membrane fouling rate increase, peaking at a salinity of 10 g/L (Phase III) and recovering back to a lower level at a salinity of 20 g/L (Phase IV). This indicated a transition occurrence at a salinity of 10 g/L (Phase III). The microbial diversity analyses further suggested a transition from salinity-sensitive microbes (Aminiphilus, Caldatribacterium, Mesotoga, Methanobrevibacter, Methanobacterium, Methanosaeta) to salinity-tolerant microbes (Longilinea, Ignavibacterium, Rhodovarius, Bosea and Flexilinea). This transition can be associated with the increase SMP concentration and more severe membrane fouling in Phase III, which were mitigated after a new equilibrium was reached when the microbial consortium acclimatized to the high salinity. Finally, a machine learning model of the Adaboost algorithm was established to predict COD removal under different salinities. Importantly, this study revealed that AnMBR process performance and membrane operation can be maintained for high salinity textile wastewater treatment with a halophilic microbial community growth under high-salinity selection pressure.

High-resolution distributed polarization crosstalk measurement for polarization maintaining fiber with considerable dispersion.

We present a high-resolution polarization crosstalk measurement method for polarization maintaining fiber (PMF) with considerable dispersion. The birefringence dispersion of the PMF severely degrades the spatial resolution of the distributed polarization crosstalk measurement. Conventional dispersion compensation methods are effective for modest birefringence dispersion coefficients (for instance, of 0.0014 ps/nm/km). We present an iterative matched filter (IMF) method to cope with the case of considerable birefringence dispersion. We measured the distributed polarization crosstalk of a PMF coil with a birefringence dispersion coefficient of 0.235 ps/nm/km. By applying the IMF method, we obtained a spatial resolution of 0.09 m at any position of the PMF (a maximum of 12.36 m without dispersion compensation).

Eph/ephrin family anchored on exosome facilitate communications between cells.

Interactions of Ephrins and Eph receptors at cell membranes play crucial role in boundary formation and axon guidance. Extracellular vesicles (EVs), such as exosomes, are formed by cells communicating with each other in paracrine or endocrine manner. Until now, it is thought that direct cell-cell contact is necessary for ephrin and Eph receptor signal transduction. In this review, we discuss recent data that indicate the existence of a novel Eph-ephrin family anchored exosome signaling pathway in long-range intercellular communication and provide evidence that this type of signaling elicits cellular responses in cancer cells, independent of juxtacrine interactions. We emphasize that exosome-anchored Eph/ephrin involves a variety of biological processes and transduction signals, which may serve as a potential diagnostic biomarker.

Symmetry evaluation for an interferometric fiber optic gyro coil utilizing a bidirectional distributed polarization measurement system.

We propose a dual-channel measurement system for evaluating the optical path symmetry of an interferometric fiber optic gyro (IFOG) coil. Utilizing a bidirectional distributed polarization measurement system, the forward and backward transmission performances of an IFOG coil are characterized simultaneously by just a one-time measurement. The simple but practical configuration is composed of a bidirectional Mach-Zehnder interferometer and multichannel transmission devices connected to the IFOG coil under test. The static and dynamic temperature results of the IFOG coil reveal that its polarization-related symmetric properties can be effectively obtained with high accuracy. The optical path symmetry investigation is highly beneficial in monitoring and improving the winding technology of an IFOG coil and reducing the nonreciprocal effect of an IFOG.

Suppression of interference noise caused by Fresnel reflection in all-fiber white-light interferometer.

We propose methods to suppress the interference noise (IN) of an all-fiber white-light interferometer (WLI), which is caused by the residual Fresnel reflective beam. The methods are proposed to ensure a wide dynamic range and enhance the accuracy of measurement. IN can cause misjudgment of the realistic optical characteristic parameters, such as the fault diagnosis and polarization coupling of the optical devices under test. In addition, IN can reduce the dynamic range of the WLI. The optical path mismatch (OPM) method and the intensity suppression (IS) method by changing the positions and intensity of the IN are presented. The two suppression methods can successfully restrain IN caused by the Fresnel reflection beams. The experimental results show that IN is successfully suppressed by the OPM method or restrained by IS method in interferograms, and the dynamic range can achieve 85 dB without IN.

Range extension of the optical delay line in white light interferometry.

We propose an effective and promising method to extend the continuous range of the optical delay line in white light interferometry. The method adopts the combination of switchable fixed delays and a continuous-scanning stage laid in one arm of the interferometer. Moreover, a fiber ring constructed by a fiber coupler is employed in the other arm to achieve self-calibration and improve the delay precision, voiding the use of a specialized measurement instrument. The range-extension method maintains the original performances of the stage just through the process of orderly copying and jointing. The setup with 16 parallel fixed delay paths is experimentally demonstrated. The insertion loss of the optical delay line will not increase as the range extension and keeps an average of 1.45 dB with a fluctuation of ±0.15 dB(3σ) over a delay range of 37.2 m without a blind zone. In addition, the delay precision as a function of the port number of the optical switch does not deteriorate and remains at a level of 10.21 µm with a standard deviation of 0.08 µm. The loss and delay precision features enable the proposed structure to be extended without limitation, which is particularly significant for practical applications.

Measurement error analysis for polarization extinction ratio of multifunctional integrated optic chips.

Measurement error for the polarization extinction ratio (PER) of a multifunctional integrated optic chip (MFIOC) utilizing white light interferometry was analyzed. Three influence factors derived from the all-fiber device (or optical circuit) under test were demonstrated to be the main error sources, including: 1) the axis-alignment angle (AA) of the connection point between the extended polarization-maintaining fiber (PMF) and the chip PMF pigtail; 2) the oriented angle (OA) of the linear polarizer; and 3) the birefringence dispersion of PMF and the MFIOC chip. Theoretical calculations and experimental results indicated that by controlling the AA range within 0°±5°, the OA range within 45°±2° and combining with dispersion compensation process, the maximal PER measurement error can be limited to under 1.4 dB, with the 3σ uncertainty of 0.3 dB. The variations of birefringence dispersion effect versus PMF length were also discussed to further confirm the validity of dispersion compensation. A MFIOC with the PER of ∼50 dB was experimentally tested, and the total measurement error was calculated to be ∼0.7 dB, which proved the effectiveness of the proposed error reduction methods. We believe that these methods are able to facilitate high-accuracy PER measurement.

Experimental proposal for performing nonlocal measurement of a product observable.

Due to the restrictions on quantum measurement imposed by relativistic causality, it is not easy to perform a nonlocal measurement on spacelike separated systems instantaneously. Here we design an experiment in an optical system to perform the nonlocal measurement of a product observable on a two-qubit system, where a maximally-entangled two-qubit state, acting as an ancillary meter, and three identical Kerr media are used to couple the system under consideration and the target meter. It is shown that the nonlocal measurement of the product observable, σzA⊗σzB, which has two degenerate eigenvalues, could be implemented in a deterministic way without violating relativistic causality.

Tracing the trajectory of photons through Fourier spectrum.

By slightly vibrating the mirrors in an interferometer at different frequencies, the photons' trajectory information is stored in the light beam. To read out this information, we record the centroid location of the intensity distribution of output beam and Fourier analyze its time evolution. It is shown that every vibrating mirror contributes a peak in the Fourier spectrum. In other words, we can reveal the trajectory of the photons by figuring out the vibrating mirrors which ever interact with the light beam based on the Fourier spectrum. This techniques is not limited by the vibration amplitude of the mirrors.

Research on Gas Multi-indicator Warning Method of Coal Mine Working Face Based on MOA-Transformer.

The gas emission from the coal mine working face is influenced by multiple factors, resulting in the real-time value, fluctuation, and trend changes of gas concentration being relatively independent and interrelated. This paper establishes a gas multi-indicator warning method that can comprehensively warn the status of real-time value, fluctuation, and trend changes of gas concentration from the working face. This paper proposes six basic indicators and includes two main research contents: intelligent threshold partition and gas multi-indicator warning. First, this paper proposes an intelligent threshold partition algorithm based on GF-KMeans (genetic fixed-centered K-means), which combines a genetic algorithm (GA) and an FC-KMeans (fixed-centered K-means) algorithm to dynamically partition the threshold range corresponding to the gas warning level. The GA solves the local optimal problem in the traditional K-Means algorithm, enhancing its stability and predictability. The FC-KMeans algorithm achieves a more precise control in the initial clustering center selection. Second, this paper researches a gas multi-indicator warning method based on a multihead optimal attention (MOA)-Transformer. By using the multihead optimization attention mechanism to represent classification features and utilizing Transformer's encoder structure to classify gas warning. The experimental result shows that the accuracy of the MOA-Transformer method is 86.17%, which is 3.45% higher than that of the Transformer method. The precision of the MOA-Transformer method is 88.78%, which is 3.75% higher than that of the Transformer method. The recall of the MOA-Transformer method is 85.23%, which is 4.70% higher than that of the Transformer method. The macro-F1 of the MOA-Transformer method is 86.96%, which is 4.39% higher than that of the Transformer method. The results fully demonstrate the superiority of the MOA-Transformer method in gas warning tasks.

Intelligent fault detection strategy for knowledge entities in fault semantic networks of distribution network based on siamese networks.

The advent of smart grid technologies has brought about a paradigm shift in the management and operation of distribution networks, allowing for intricate system information to be encapsulated within semantic network models. These models, while robust, are not immune to faults within their knowledge entities, which can arise from a myriad of issues, potentially leading to verification failures and operational disruptions. Addressing this critical vulnerability, our research delves into the development of a novel fault detection methodology specifically tailored for the knowledge entity variables of semantic networks in distribution networks. In our approach, we first construct a state space equation that models the behavior of knowledge entity variables in the presence of faults. This foundational framework enables us to apply an unknown input observer strategy to effectively detect anomalies within the system. To bolster the fault identification process, we introduce the innovative use of a siamese network, a neural network architecture which is proficient in differentiating between similar datasets. Through simulation scenarios, we demonstrate the efficacy of our proposed fault detection method.

Morphology regulation of conductive metal-organic frameworks in situ grown on graphene oxide for high-performance supercapacitors.

In this work, nickel-catecholate (Ni-CAT) nanorods were in situ compounded on graphene oxide (GO) to form a composite Ni-CAT@GO (NCG) with a special "blanket-shape" structure, which was used as an electrode material for supercapacitors. The morphology of Ni-CATs in situ grown on GO was modulated by introducing various contents of GO. With increasing GO, the length of nanorods of Ni-CATs is obviously shortened, and the charge transfer resistance of NCG is significantly reduced as the GO content is relatively low while it increases with further addition of GO, because excessive GO in NCG results in smaller crystal sizes accompanied by smaller stacking pores. Both the over-long Ni-CAT nanorods and the smaller stacking pores can restrict the accessible surface areas for the electrolyte. Optimal nanorod sizes are crucial to achieve good electrochemical performance for electrode materials. Galvanostatic charge-discharge analysis of NCG electrodes shows that their capacity initially increases and then decreases with the addition of more and more GO, and Ni-CAT@GO-0.5 (NCG0.5) with minimal charge transfer resistance exhibits the best electrochemical performance. The results demonstrate that the NCG0.5 electrode with optimal morphology possesses an excellent capacitance of 563.8 F g-1 at 0.5 A g-1 and a good rate performance of 61.9% at 10 A g-1, indicating that Ni-CAT@GO is a new type of promising electrode material for supercapacitors based on conductive metal-organic frameworks.