RESUMEN
A rare transthoracic echocardiographic image of left sinus of Valsalva aneurysm complicated by thrombus formation.
Asunto(s)
Aneurisma de la Aorta , Ecocardiografía , Seno Aórtico , Trombosis , Humanos , Seno Aórtico/diagnóstico por imagen , Trombosis/diagnóstico por imagen , Trombosis/complicaciones , Aneurisma de la Aorta/diagnóstico por imagen , Aneurisma de la Aorta/complicaciones , Ecocardiografía/métodos , Masculino , Diagnóstico DiferencialRESUMEN
A 35-year-old woman was initially misdiagnosed with a muscular ventricular septal defect but was later correctly diagnosed with a double-chambered left ventricle following evaluation by echocardiography and cardiac computed tomography.
Asunto(s)
Errores Diagnósticos , Ecocardiografía , Defectos del Tabique Interventricular , Ventrículos Cardíacos , Humanos , Femenino , Defectos del Tabique Interventricular/diagnóstico por imagen , Defectos del Tabique Interventricular/diagnóstico , Adulto , Ventrículos Cardíacos/diagnóstico por imagen , Ventrículos Cardíacos/anomalías , Ecocardiografía/métodos , Diagnóstico Diferencial , Tomografía Computarizada por Rayos X/métodosRESUMEN
Based on the One Strain-Many Compounds (OSMAC) strategy, the secondary metabolites of Phomopsis lithocarpus FS508 were investigated. As a result, a new secondary metabolite, 4-methoxy-3-[4-(acetyloxy)-3-methyl-2-butenyl]benzoic acid (1) as well as eleven known compounds were isolated from the fermentation product of the strain FS508. Their structures were determined by NMR, IR, UV, and MS spectroscopic data analyses. All the isolated compounds were evaluated for cytotoxic and anti-inflammatory activities. Among them, compounds 3 and 9 displayed potent cytotoxicity against HepG-2 cell line, and compounds 2, 3 and 12 showed significant anti-inflammatory activities.
Asunto(s)
Antineoplásicos , Ascomicetos , Phomopsis , Ascomicetos/química , Línea Celular Tumoral , Antineoplásicos/química , Antiinflamatorios/farmacología , Estructura MolecularRESUMEN
Four new phomalones A-D (1-4), together with five known analogues (5-9) were isolated from the deep-sea-derived fungus Trichobotrys effuse FS522. Their structures of the new compounds established by analysis of their NMR and HR-ESI-MS spectroscopic data, and the absolute configurations of 2 was determined by electronic circular dichroism (ECD) calculations. compounds 4, 6 and 8 substantially inhibited the production of nitric oxide (NO) with IC50 values of 4.64, 13.90, and 34.07â µM.
Asunto(s)
Ascomicetos , Antiinflamatorios/farmacología , Espectroscopía de Resonancia Magnética/métodos , Estructura Molecular , Piranos/química , Piranos/farmacología , Compuestos Heterocíclicos con 3 Anillos/química , Compuestos Heterocíclicos con 3 Anillos/farmacologíaRESUMEN
The development of drug-resistance in the opportunistic pathogen Escherichia coli has become a global public health concern. Due to the share of similar flora between pets and their owners, the detection of pet-origin antibiotic-resistant E. coli is necessary. This study aimed to detect the prevalence of feline-origin ESBL E. coli in China and to explore the resistance elimination effect of garlic oil to cefquinome on ESBL E. coli. Cat fecal samples were collected from animal hospitals. The E. coli isolates were separated and purified by indicator media and polymerase chain reaction (PCR). ESBL genes were detected by PCR and Sanger sequencing. The MICs were determined. The synergistic effect of garlic oil and cefquinome against ESBL E. coli was investigated by checkerboard assays, time-kill and growth curves, drug-resistance curves, PI and NPN staining, and a scanning electronic microscope. A total of 80 E. coli strains were isolated from 101 fecal samples. The rate of ESBL E. coli was 52.5% (42/80). The prevailing ESBL genotypes in China were CTX-M-1, CTX-M-14, and TEM-116. In ESBL E. coli, garlic oil increased the susceptibility to cefquinome with FICIs from 0.2 to 0.7 and enhanced the killing effect of cefquinome with membrane destruction. Resistance to cefquinome decreased with treatment of garlic oil after 15 generations. Our study indicates that ESBL E. coli has been detected in cats kept as pets. The sensitivity of ESBL E. coli to cefquinome was enhanced by garlic oil, indicating that garlic oil may be a potential antibiotic enhancer.
Asunto(s)
Infecciones por Escherichia coli , Escherichia coli , Gatos , Animales , Infecciones por Escherichia coli/tratamiento farmacológico , Infecciones por Escherichia coli/veterinaria , Infecciones por Escherichia coli/epidemiología , Farmacorresistencia Bacteriana/genética , Antibacterianos/farmacología , beta-Lactamasas/genéticaRESUMEN
Six new α-pyrone meroterpenoid chevalones H-M (1-6), together with six known compounds (7-12), were isolated from the gorgonian coral-derived fungus Aspergillus hiratsukae SCSIO 7S2001 collected from Mischief Reef in the South China Sea. Their structures, including absolute configurations, were elucidated on the basis of spectroscopic analysis and X-ray diffraction data. Compounds 1-5 and 7 showed different degrees of antibacterial activity with MIC values of 6.25-100 µg/mL. Compound 8 exhibited potent cytotoxicity against SF-268, MCF-7, and A549 cell lines with IC50 values of 12.75, 9.29, and 20.11 µM, respectively.
Asunto(s)
Antozoos , Antibacterianos/farmacología , Antineoplásicos/farmacología , Aspergillus , Pironas/farmacología , Animales , Antibacterianos/química , Antineoplásicos/química , Organismos Acuáticos , Línea Celular Tumoral , China , Humanos , Concentración 50 Inhibidora , Pruebas de Sensibilidad Microbiana , Pironas/químicaRESUMEN
Benzophenones are polyketides with diverse biological activities. Novel cytotoxic benzophenones cytosporaphenones A-C and cytorhizins A-D, which contain a new skeleton, were previously extracted from endophytic fungus Cytospora rhizophorae A761. However, the mechanism for the biosynthesis of these compounds remains unknown. Cytosporaphenone A was assumed to be the precursor for the biosynthesis of cytorhizins A-D. In this study, we sequenced the genome of C. rhizophorae A761 and characterized a benzoate 4-monooxygenase cytochrome P450(BAM). CRISPR/Cas9-mediated gene knockout and overexpression studies in C. rhizophorae confirmed the vital function of BAM in the biosynthesis of cytosporaphenones and cytorhizins. Overexpression of BAM also enhanced the yield of cytosporaphenone A by 1.868 folds. The in vitro function and enzymatic properties of BAM were also described. This study demonstrates the important role of BAM for the biosynthesis of cytosporaphenone A and cytorhizins and is also the first to provide approaches for the CRISPR-Cas9-mediated gene deletion and gene overexpression studies in C. rhizophoarae, thus laying a foundation for the elucidation of the biosynthetic mechanism of cytorhizins and the discovery of new benzophenones mediated by BAM.Key points⢠The novel bam gene encoding BAM protein in C. rhizophorae was firstly deleted using CRIPSR/Cas9 system.⢠The in vitro oxidation function of novel BAM protein and enzymatic properties was characterized.⢠The over expression of bam gene enhanced the yield of cytosporaphone A in C. rhizophorae significantly.
Asunto(s)
Ascomicetos , Policétidos , Ascomicetos/genética , Benzofenonas , Sistema Enzimático del Citocromo P-450/genéticaRESUMEN
To enlarge the chemical diversity of Eurotium sp. SCSIO F452, a talented marine-derived fungus, we further investigated its chemical constituents from a large-scale fermentation with modified culture. Four pairs of new salicylaldehyde derivative enantiomers, euroticins F-I (1-4), as well as a known one eurotirumin (5) were isolated and characterized. Compound 1 features an unprecedented constructed 6/6/6/5 tetracyclic structures, while 2 and 3 represent two new types of 6/6/5 scaffolds. Their structures were established by comprehensive spectroscopic analyses, X-ray diffraction, 13C NMR, and electronic circular dichroism calculations. Selected compounds showed significant inhibitory activity against α-glucosidase and moderate cytotoxic activities against SF-268, MCF-7, HepG2, and A549 cell lines.
Asunto(s)
Aldehídos/farmacología , Antineoplásicos/farmacología , Antioxidantes/farmacología , Eurotium , Aldehídos/química , Animales , Antineoplásicos/química , Antioxidantes/química , Organismos Acuáticos , Línea Celular Tumoral/efectos de los fármacos , Humanos , Estructura Molecular , EstereoisomerismoRESUMEN
Four novel benzophenone derivatives, cytosporins A-D (1-4), hemiterpene-conjugated phenolics with an unprecedented benzo[b][1,5]dioxocane skeleton, were isolated from Cytospora rhizophorae A761. The structures of the new compounds were fully characterized on the basis of extensive spectroscopic analysis. The deduced structure represents the first example of natural meroterpenoids which bear a benzo[b][1,5]dioxocane framework embodying hemiterpene and benzophenone moieties. Moreover, compounds 1-4 were evaluated for in vitro antimicrobial activity.
Asunto(s)
Antibacterianos/química , Antibacterianos/farmacología , Ascomicetos/química , Benzofenonas/química , Benzofenonas/farmacología , Ciclosporinas/química , Ciclosporinas/farmacología , Antibacterianos/metabolismo , Ascomicetos/metabolismo , Benzofenonas/metabolismo , Cristalografía por Rayos X , Ciclosporinas/metabolismo , Escherichia coli/efectos de los fármacos , Infecciones por Escherichia coli/tratamiento farmacológico , Hemiterpenos/química , Hemiterpenos/metabolismo , Hemiterpenos/farmacología , Humanos , Modelos Moleculares , Fenoles/química , Fenoles/metabolismo , Fenoles/farmacología , Infecciones Estafilocócicas/tratamiento farmacológico , Staphylococcus aureus/efectos de los fármacosRESUMEN
A fistula between the pulmonary artery (PA) and the left atrium (LA) is a rare congenital heart disease that usually presents with cyanosis, clubbing, and dyspnea, as well as the signs and symptoms of a right-to-left shunt. Herein, we report a 16-year-old girl with a fistula between the right PA and the LA. This type of fistula could lead to systemic desaturation. This patient also had an atrial septal defect of the secundum type and has been followed up without treatment. The clinical manifestations and treatment of fistulas located between the PA and LA are also reviewed in this report.