Food web structure of fish communities of Doce River, 5 years after the Fundão dam failure.

The rupture of the Fundão dam is considered the largest mining failure in history, which had a particularly detrimental impact on fish populations, as the mud from the ore tailings significantly altered the water quality and habitat of Doce River basin. This study aimed to assess the trophic structure of fish communities in areas impacted and not impacted by the dam rupture in the Doce River basin. To evaluate the food web structure, community-wide trophic niche, and trophic positions of fish, stable isotopes of carbon (δ13C) and nitrogen (δ15N) were utilized across ten sites (seven impacted and three control). In general, fish appeared to assimilate resources such as invertebrates, algae, and periphyton, although the importance of each resource varied among sites. The site closest to the dam rupture exhibited a more simplified trophic structure compared to the control sites and those nearer the river mouth. In this site, most fish species occupied a similar trophic position. Trophic niches also exhibited the greatest dissimilarity between the site closest to the dam failure and those farther away from it, with an expansion of trophic niche breadth observed with an increase in the distance from the dam rupture. Our study provided valuable insights into the trophic structure of fish communities within the Doce River basin, shedding light on the trophic ecology of the 59 fish species investigated. We also emphasize the importance of our study for future assessments of ore tailings dam failure disasters and evaluating the effectiveness of mitigation measures for Doce River basin recovery.

Nitrogen pollution promotes changes in the niche space of fish communities.

Historically, anthropogenic fixed nitrogen has been purposely increased to benefit food production and global development. One consequence of this increase has been to raise concentrations of nitrogen in aquatic ecosystems. To evaluate whether nitrogen pollution promotes changes in the estimates of niche space of fish communities, we examined 16 sites along a Brazilian river basin highly impacted by anthropogenic activities, especially discharge of domestic and industrial sewage from a region with more than 5 million inhabitants. We analysed the carbon (δ13C) and nitrogen (δ15N) isotope ratios of fish species and both autochthonous (periphyton) and allochthonous (course and fine particulate organic matter) basal food resources. To estimate the magnitude of nitrogen pollution, we measured the nitrate and ammonium concentrations at each site. Sampling was conducted in the dry and wet seasons to evaluate the influence of seasonality. Nitrogen pollution generally increased estimates of niche space, and seasonality influenced only the niche estimates of fish communities from polluted sites. In addition, isotopic analyses of nitrogen polluted sites yielded unrealistic estimates of trophic positioning (detritivores at the top of the food web). We conclude that changes in niche space estimates reflect both alterations in baseline isotopic values and differential trophic behaviour among fishes. Our study suggests that under conditions of high pollution, other factors appear to influence isotopic estimates of niche, such as isotopically distinct sources that have not been sampled, and/or differences in δ15N turnover rates between fish tissue and basal resources, creating isotopic baselines that are challenging to interpret.

Ecosystem variables importance in the presence and abundance of a globally invasive fish.

Changes in physical habitat that are associated with anthropogenic disturbances facilitate the establishment and expansion of non-native species in receiving environments. Here, we evaluated the relative importance of ecosystem variables for the presence and abundance of the invasive fish Poecilia reticulata in Brazil. We collected fish species and assessed environmental variables through an established physical habitat protocol in 220 stream sites located in southeastern and midwestern Brazil. A total of 14,816 P. reticulata individuals were collected in 43 stream sites, and 258 variables that describe the physical characteristics of streams were assessed, including measures of channel morphology, substrate size and type, habitat complexity and cover, riparian vegetation cover and structure, and human influence. Dimensionality reduction methods were employed to limit redundancy, resulting in a smaller set of the most relevant environmental variables. Subsequently, we used random forest models to assess the relative importance of these variables in determining the presence and abundance of P. reticulata. The presence of this invasive fish was primarily explained by human disturbance variables related to urbanization (total impact, pavement, artificial structure areal cover, riparian canopy cover, electrical conductivity, mean thalweg depth, and sand), whereas channel morphology (mean bank full height) and fish cover variables (natural fish cover, and aquatic macrophyte areal cover) were important predictors of its abundance. Identifying which ecosystem variables are favorable to the establishment of non-native species is an important step in preventing future biological invasions, as well as managing those that already occur.

Trophic diversity and carbon sources supporting fish communities along a pollution gradient in a tropical river.

Anthropogenic activities can have a great influence on water quality and in the availability of habitat and food resources, which can promote changes in the trophic diversity and carbon sources sustaining aquatic communities. The objective of this study was to evaluate if the trophic diversity and the main carbon sources sustaining fish communities change along a pollution gradient. The study was carried out at eight sites distributed along the Rio das Velhas, a Brazilian river highly impacted by anthropogenic activities, in which the discharge of domestic and industrial sewage from the Metropolitan Region of Belo Horizonte (MRBH) presents a major source of pollution. Using carbon (δ13C) and nitrogen (δ15N) isotope ratios, we identified the major carbon sources/food sources of common fish species and calculated six metrics of trophic diversity. Autochthonous primary producers (algae, periphyton, and macrophytes) were the main carbon sources for all trophic guilds at all sites, but notably, sewage-derived organic matter was an additional significant carbon source to the fish community in the most polluted testing site. Here, the community was composed mainly by detritivorous and omnivorous fishes and exhibited greater ranges of carbon and nitrogen isotopic values, large total areas, high trophic diversity, small trophic redundancy, and less even distribution of trophic niches than the less polluted sites. We conclude that the trophic guilds, trophic diversity metrics, and carbon sources sustaining fish communities in the Rio das Velhas are highly influenced by the presence of pollution. Besides favoring omnivorous and detritivorous fishes, the input of sewage also provided an important food source to sustain the fish community from sites close to the MRBH.

Trophic responses to aquatic pollution of native and exotic livebearer fishes.

The objective of this study was to evaluate if aquatic pollution promote diet shifts in two livebearer fishes (Poeciliidae): an exotic species, the guppy (Poecilia reticulata), and a native livebearer (Phalloceros uai). The study was carried out in a Brazilian basin highly impacted by anthropogenic activities, especially discharge of domestic and industrial sewage from a region with more than five million human inhabitants. To evaluate the trophic ecology of both native and exotic species it was analysed carbon (δ13C) and nitrogen (δ15N) stable isotopes of fish tissue, food resources and, sewage. Moreover, stable isotopes analyses were coupled with gut contents of the two species to provide additional information about fish diet. Exotic guppy abundance was high in the most polluted site, where P. reticulata assimilated carbon directly from sewage. The native species was absent in the most polluted site, but presented wider niches than the exotic species in almost all other sites. Gut content analyses indicated high consumption of aquatic insects by both species. However, while the native species consumed a diverse suite of insect taxa, the exotic species consumed mainly Chironomidae larvae. We conclude that aquatic pollution promotes diet shifts in both native and exotic species, with both species changing their trophic niches in a similar way according to the level of degradation of the environment. The ability to directly assimilate sewage, together with its capacity to survive in environments with poor water quality and its reproductive strategy, may favour the establishment of exotic guppies in strongly polluted sites.