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1.
Brain ; 132(Pt 11): 3060-71, 2009 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-19498089

RESUMEN

Magnetoencephalography (MEG) is considered a useful tool for planning electrode placement for chronic intracranial subdural electrocorticography (ECoG) in candidates for epilepsy surgery or even as a substitute for ECoG. MEG recordings are usually interictal and therefore, at best, reflect the interictal ECoG. To estimate the clinical value of MEG, it is important to know how well interictal MEG reflects interictal activity in the ECoG. From 1998 to 2008, 38 candidates for ECoG underwent a 151-channel MEG recording and 3D magnetic resonance imaging as a part of their presurgical evaluation. Interictal MEG spikes were identified, clustered, averaged and modelled using the multiple signal classification algorithm and co-registered to magnetic resonance imaging. ECoG was continuously recorded with electrode grids and strips for approximately 1 week. In a representative sample of awake interictal ECoG, interictal spikes were identified and averaged. The different spikes were characterized and quantified using a combined amplitude and synchronous surface-area measure. The ECoG spikes were ranked according to this measure and plotted on the magnetic resonance imaging surface rendering. Interictal spikes in MEG and ECoG were allocated to a predefined anatomical brain region and an association analysis was performed. All interictal MEG spikes were associated with an interictal ECoG spike. Overall, 56% of all interictal ECoG spikes had an interictal MEG counterpart. The association between the two was >or=90% in the interhemispheric and frontal orbital region, approximately 75% in the superior frontal, central and lateral temporal regions, but only approximately 25% in the mesial temporal region. MEG is a reliable indicator of the presence of interictal ECoG spikes and can be used to plan intracranial electrode placements. However, a substantial number of interictal ECoG spikes are not detected by MEG, and therefore MEG cannot be considered a substitute for ECoG.


Asunto(s)
Mapeo Encefálico/métodos , Electroencefalografía/métodos , Epilepsia/fisiopatología , Epilepsia/cirugía , Magnetoencefalografía/métodos , Adolescente , Adulto , Encéfalo/anatomía & histología , Encéfalo/fisiología , Encéfalo/fisiopatología , Mapeo Encefálico/instrumentación , Niño , Preescolar , Electrodos Implantados , Epilepsia/patología , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven
2.
Acta Neurol Scand ; 119(3): 199-206, 2009 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-18684215

RESUMEN

OBJECTIVE: In the Netherlands, presurgical screening for temporal lobe epilepsy (TLE) includes the intracarotid amobarbital procedure (IAP), consisting of two consecutive injections of amobarbital, ipsilateral and contralateral to the epileptic focus. We studied whether a bilateral IAP has added value to a unilateral, ipsilateral IAP. METHODS: This population-based study included 183 consecutive patients referred for screening for TLE surgery who underwent bilateral IAP. Using multivariable modeling, we assessed the added value of bilateral IAP on the decision for surgery, resection size, amygdalohippocampectomy, post-operative seizure freedom, memory performance, and IQ change. RESULTS: Given the results from the unilateral IAP, the bilateral IAP had added prognostic value for postoperative change in verbal memory (P < 0.01) and verbal IQ (P < 0.01), especially if patients had a left-sided focus. In contrast, information provided by the contralateral IAP was not associated with decision-making or surgical strategy. CONCLUSIONS: A bilateral IAP has added value in predicting post-operative verbal memory and IQ. A bilateral IAP is currently not used to guide surgical strategy, but may be used for this purpose when verbal capacity is of particular concern in patients with a left-sided focus. In other cases, IAP is best performed unilaterally.


Asunto(s)
Amnesia/prevención & control , Amobarbital , Dominancia Cerebral , Epilepsia del Lóbulo Temporal/cirugía , Complicaciones Posoperatorias/prevención & control , Adolescente , Amobarbital/administración & dosificación , Afasia/prevención & control , Arteria Carótida Interna , Niño , Preescolar , Femenino , Humanos , Inyecciones Intraarteriales , Pruebas de Inteligencia , Pruebas del Lenguaje , Masculino , Países Bajos , Pruebas Neuropsicológicas , Procedimientos Neuroquirúrgicos , Cuidados Preoperatorios , Pronóstico , Estudios Retrospectivos , Procedimientos Innecesarios
3.
Seizure ; 16(5): 445-53, 2007 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-17412615

RESUMEN

INTRODUCTION: Epilepsy associated with tuberous sclerosis complex (TSC) is drug resistant in more than half of the patients. Epilepsy surgery may be an alternative treatment option, if the epileptogenic tuber can be identified reliably and if seizure reduction is not at the expense of cognitive or other functions. We report the pre-surgical identification of the epileptogenic tuber and post-surgical outcome of patients with TSC in The Netherlands. METHODS: Twenty-five patients underwent the pre-surgical evaluation of the Dutch Comprehensive Epilepsy Surgery Programme, including a detailed seizure history, interictal and ictal video EEG registrations, 3D FLAIR MRI scans and neuropsychological testing. Suitability of the candidates was decided in consensus. Seizure outcome, scored with the Engel classification, and cognition were reassessed at fixed post-surgery intervals. RESULTS: Epilepsy surgery was performed in six patients. At follow-up, four patients had Engel classification 1, two had classification 4. Improved development and behaviour was perceived by the parents of two patients. Epilepsy surgery was not performed in 19 patients because seizures were not captured, ictal onset zones could not be localised or were multiple, interictal EEG, video EEG and MEG results were not concordant, or seizure burden had diminished during decision making. A higher cognition index was found in the surgical patients compared to the non-surgical candidates. CONCLUSIONS: Epilepsy surgery can be performed safely and successfully in patients in whom semiology, interictal EEG, ictal EEG, MEG and the location of tubers are concordant. In other cases the risk of surgery should be weighed against the chance of seizure relief and in case of children subsequent impact on neurodevelopment.


Asunto(s)
Epilepsia/complicaciones , Epilepsia/cirugía , Procedimientos Neuroquirúrgicos/métodos , Resultado del Tratamiento , Esclerosis Tuberosa/complicaciones , Adolescente , Adulto , Niño , Preescolar , Electroencefalografía/métodos , Femenino , Estudios de Seguimiento , Humanos , Imagen por Resonancia Magnética , Magnetoencefalografía , Masculino , Países Bajos/epidemiología , Pruebas Neuropsicológicas , Estudios Retrospectivos
4.
Ned Tijdschr Geneeskd ; 150(43): 2378-85, 2006 Oct 28.
Artículo en Holandés | MEDLINE | ID: mdl-17100130

RESUMEN

OBJECTIVE: Description of initial experiences with subdural electrode grids in patients with refractory focal epilepsy as additional diagnostic tool for epilepsy surgery. Using these electrodes, the attacks were recorded during a number of days and the cerebral cortex was electrically stimulated in order to map the functional areas. DESIGN: Retrospective. METHOD: Data were collected from patients in whom subdural electrode grids had been placed between 1 September 1999 and 31 August 2004. All patients underwent a neurological examination and a neuropsychological test before the implantation. At the follow-up examination, the results with regard to function and the frequency of attacks were noted, as well as the complications. RESULTS: Electrodes were placed in 22 patients: 9 women and 13 men with an average age of 27 years (range: 5-42). The implantation lasted for an average of 7 days (range: 3-10). In 4 patients, increased seizures during implantation required intravenous anticonvulsant treatment. Severe but transitory complications were seen in 4 patients (meningitis, subdural haematoma and ischaemia). 19 patients underwent a therapeutic resection. A postoperative decline in language skills was noted in 1 patient, while another 2 scored poorer in verbal tests. A permanent decline in sensorimotor function was seen in 1 patient, but this had been foreseen. Of the 16 operated patients with a duration of follow-up of at least 1 year, so were (practically) free of attacks, and another 3 patients had significantly fewer attacks. CONCLUSION: Registration with intracranial electrodes makes it possible to treat epileptic patients surgically by excision of brain tissue near critical areas. Such intensive monitoring is, however, not without risk and this must be weighed against the potential benefits.


Asunto(s)
Encéfalo/fisiopatología , Electrodos Implantados , Electroencefalografía/métodos , Epilepsia/cirugía , Adolescente , Adulto , Encéfalo/cirugía , Niño , Preescolar , Electrodos Implantados/efectos adversos , Femenino , Humanos , Masculino , Estudios Retrospectivos , Resultado del Tratamiento
5.
Arch Dis Child Fetal Neonatal Ed ; 90(3): F245-51, 2005 May.
Artículo en Inglés | MEDLINE | ID: mdl-15846017

RESUMEN

OBJECTIVE: To assess the time course of recovery of severely abnormal initial amplitude integrated electroencephalographic (aEEG) patterns (flat trace (FT), continuous low voltage (CLV), or burst suppression (BS)) in full term asphyxiated neonates, in relation to other neurophysiological and neuroimaging findings and neurodevelopmental outcome. METHODS: A total of 190 aEEGs of full term infants were reviewed. The neonates were admitted within 6 hours of birth to the neonatal intensive care unit because of perinatal asphyxia, and aEEG recording was started immediately. In all, 160 infants were included; 65 of these had an initial FT or CLV pattern and 25 an initial BS pattern. Neurodevelopmental outcome was assessed using a full neurological examination and the Griffiths' mental developmental scale. RESULTS: In the FT/CLV group, the background pattern recovered to continuous normal voltage within 24 hours in six of the 65 infants (9%). All six infants survived the neonatal period; one had a severe disability, and five were normal at follow up. In the BS group, the background pattern improved to normal voltage in 12 of the 25 infants (48%) within 24 hours. Of these infants, one died, five survived with moderate to severe disability, two with mild disability, and four were normal. The patients who did not recover within 24 hours either died in the neonatal period or survived with a severe disability. CONCLUSION: In this study there was a small group of infants who presented with a severely abnormal aEEG background pattern within six hours of birth, but who achieved recovery to a continuous normal background pattern within the first 24 hours. Sixty one percent of these infants survived without, or with a mild, disability.


Asunto(s)
Asfixia Neonatal/fisiopatología , Electroencefalografía , Asfixia Neonatal/complicaciones , Parálisis Cerebral/etiología , Discapacidades del Desarrollo/etiología , Evaluación de la Discapacidad , Métodos Epidemiológicos , Humanos , Recién Nacido , Cuidado Intensivo Neonatal/métodos , Pronóstico , Procesamiento de Señales Asistido por Computador
6.
Diabetes Care ; 16(9): 1249-53, 1993 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-8404428

RESUMEN

OBJECTIVE: We investigated whether the corrected QT interval correlated with two other tests for diagnosing autonomic dysfunction in 60 type I diabetic patients with proven peripheral neuropathy. The mean age +/- SD was 48.3 +/- 11.2 yr, the mean duration of diabetes was 24.9 +/- 11.4 yr, and the mean HbA1 was 9.3 +/- 2.4%. RESEARCH DESIGN AND METHODS: All patients underwent three autonomic function tests: 1) the standard five cardiovascular Ewing tests, each scored 0 (normal), 0.5 (borderline), or 1.0 (abnormal). We used the sum of the abnormal findings for the analysis, the cardiovascular autonomic score; 2) measurement of the corrected QT interval taken from a routine electrocardiogram recording; and 3) static and dynamic pupillometry: measurement of dark adapted pupil diameter as percentage of total iris diameter and of pupil constriction latency using an infrared light reflex technique. RESULTS: No significant correlation was found between age, duration of diabetes, or HbA1 and any of the autonomic function tests, except for one between age and cardiovascular autonomic score (r = 0.3202, P = 0.0126). Corrected QT interval did not correlate with cardiovascular autonomic score, pupil diameter, or constriction latency. A significant inverse correlation was found between cardiovascular autonomic score and pupil diameter (r = -0.4861, P < 0.001) and constriction latency (r = 0.3783, P < 0.001). Pupil diameter and constriction latency correlated well (r = -0.4276, P < 0.001). CONCLUSIONS: The corrected QT interval did not correlate with cardiovascular autonomic tests nor pupillometry results. The corrected QT interval therefore should not be used for the diagnosis of the severity of diabetic autonomic neuropathy.


Asunto(s)
Diabetes Mellitus Tipo 1/fisiopatología , Neuropatías Diabéticas/fisiopatología , Electrocardiografía , Sistema Nervioso Autónomo/fisiopatología , Femenino , Hemoglobina Glucada/análisis , Frecuencia Cardíaca , Humanos , Masculino , Persona de Mediana Edad , Reflejo Pupilar , Análisis de Regresión , Maniobra de Valsalva
7.
Stroke ; 32(3): 728-34, 2001 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-11239194

RESUMEN

BACKGROUND AND PURPOSE: We sought to investigate whether in patients with a symptomatic internal carotid artery (ICA) occlusion, endarterectomy of a severe stenosis of the contralateral carotid artery can establish long-term cerebral hemodynamic improvement. METHODS: Nineteen patients were studied on average 1 month before and 6 months after contralateral carotid endarterectomy (CEA). Volume flow in the main extracranial and intracranial arteries was measured with MR angiography. Collateral flow via the circle of Willis and the ophthalmic arteries was studied with MR angiography and transcranial Doppler sonography, respectively. Cerebral metabolism and CO(2) vasoreactivity were investigated with MR spectroscopy and transcranial Doppler sonography, respectively. Twelve nonoperated patients with a symptomatic ICA occlusion and contralateral ICA stenosis, who were matched for age and sex, served as control patients. RESULTS: In patients who underwent surgery, flow in the operated ICA increased significantly (P:<0.05) and flow in the basilar artery decreased significantly (P:<0.01) after CEA. On the occlusion side, mean flow in the middle cerebral artery increased significantly from 71 to 85 mL/min (P:<0.05) after CEA. The prevalence of collateral flow via the anterior communicating artery to the occlusion side increased significantly (47% before and 84% after CEA; P:<0.05), while the prevalence of reversed ophthalmic artery flow on the operation side decreased significantly (42% before and 5% after CEA; P:<0.05). In the hemisphere on the side of the ICA occlusion, lactate was no longer detected after CEA in 80% of operated patients, whereas it was no longer detected over time in 14% of nonoperated patients (P:<0.05). CO(2) reactivity increased significantly in operated patients in both hemispheres (P:<0.01). CONCLUSIONS: Contralateral CEA in patients with a symptomatic ICA occlusion induces cerebral hemodynamic improvement not only on the side of surgery but also on the side of the ICA occlusion.


Asunto(s)
Ácido Aspártico/análogos & derivados , Estenosis Carotídea/cirugía , Circulación Cerebrovascular , Endarterectomía Carotidea , Hemodinámica , Ácido Aspártico/metabolismo , Arteria Basilar/diagnóstico por imagen , Arteria Basilar/patología , Velocidad del Flujo Sanguíneo , Encéfalo/irrigación sanguínea , Encéfalo/metabolismo , Dióxido de Carbono/metabolismo , Arteria Carótida Interna/diagnóstico por imagen , Arteria Carótida Interna/patología , Arteria Carótida Interna/cirugía , Estenosis Carotídea/diagnóstico , Estenosis Carotídea/patología , Arterias Cerebrales/diagnóstico por imagen , Colina/metabolismo , Circulación Colateral , Femenino , Humanos , Ácido Láctico/metabolismo , Angiografía por Resonancia Magnética , Masculino , Persona de Mediana Edad , Arteria Oftálmica/diagnóstico por imagen , Resultado del Tratamiento , Ultrasonografía Doppler Transcraneal
8.
Arch Neurol ; 46(4): 445-7, 1989 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-2495786

RESUMEN

There is a tendency to call benign focal epilepsy of childhood with centrotemporal origin rolandic epilepsy for the sake of brevity. Martinus Rulandus, a 16th-century medical author, deserves this homage far more than Rolando. Attention is drawn to this 1597 description of the disease, which has become the subject of many publications since 1952. Many characteristics of the disease were already noticed about four centuries ago.


Asunto(s)
Epilepsias Parciales/historia , Neurología/historia , Niño , Alemania , Historia del Siglo XVI , Historia del Siglo XX , Humanos , Italia , Literatura Moderna
9.
Neurology ; 55(12): 1806-12, 2000 Dec 26.
Artículo en Inglés | MEDLINE | ID: mdl-11134377

RESUMEN

OBJECTIVE: To identify hemodynamic factors that predict recurrence of ipsilateral cerebral ischemic events in patients with symptomatic carotid artery occlusion (CAO). PATIENTS AND METHODS: The authors studied 117 consecutive patients with CAO and corresponding recent (

Asunto(s)
Arteriopatías Oclusivas/fisiopatología , Enfermedades de las Arterias Carótidas/fisiopatología , Hemodinámica/fisiología , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Pronóstico , Recurrencia , Factores de Riesgo
10.
Invest Radiol ; 36(4): 225-33, 2001 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-11283420

RESUMEN

RATIONALE AND OBJECTIVES: To compare the visibility and localization of extratemporal cortical lesions in extratemporal epilepsy by using curved reconstruction (CR) and three-dimensional surface rendering (3D SR) of 3D-acquired MR images and to study the degree of confidence with which localizations are made, particularly at the gyral level. METHODS: Twenty patients with extratemporal epilepsy, based on seizure symptomatology and/or scalp electroencephalographic registrations, with an extratemporal structural lesion on conventional MR imaging, were selected for this study by a neuroradiologist with extensive experience in the assessment of epilepsy patients. Transverse T2 spin-echo, coronal fluid-attenuated inversion recovery, and transverse 3D-acquired/two-dimensionally reconstructed T1 MR images were used for the selection. A second neuroradiologist (observer 1) and a radiology resident (observer 2) assessed CR and 3D SR in random order. Both observers were masked to all patient data. The subjective visibility of lesions and gyral location were scored. The interobserver agreements for lesion visibility and localization and for degree of confidence were compared for CR and 3D SR. RESULTS: For both observers, the lesion was visible in 55% of 3D SRs and 95% of CRs. The proportion with "very clearly visible" lesions on 3D SR was 19% (4/20) according to observer 1 and 30% (6/20) according to observer 2. For CR, this proportion was substantially higher: 55% for both observers. This difference was significant for observer 1 but not for observer 2. The interobserver agreement was high for both methods. Agreement on gyral localization was 28% for CR and 40% for 3D SR. The percentage of similar confidence scores for the same gyral localization and for gyral localization with a maximum difference of one gyrus between the observers did not differ significantly for CR or 3D SR. The observers were more often confident in agreed cases in CR and moderately confident in 3D SR. CONCLUSIONS: These results suggest that CRs of the brain surface are superior to 3D SR for the visualization of extratemporal cortical lesions in patients with drug-resistant extratemporal epilepsy. If lesions are seen, no significant difference was found between the two techniques for localization; however, the degree of confidence appears higher for CR at the gyral level.


Asunto(s)
Corteza Cerebral/patología , Epilepsia/patología , Procesamiento de Imagen Asistido por Computador , Imagen por Resonancia Magnética , Adolescente , Adulto , Niño , Preescolar , Femenino , Humanos , Imagenología Tridimensional , Masculino , Persona de Mediana Edad , Variaciones Dependientes del Observador
11.
Invest Radiol ; 34(1): 38-45, 1999 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-9888052

RESUMEN

RATIONALE AND OBJECTIVES: To evaluate the diagnostic relevance of ipsilateral atrophy of the collateral white matter in the parahippocampal gyrus (ACWMp) and temporal lobe gray/white matter demarcation loss (GWDL) on magnetic resonance imaging in patients with histologically confirmed hippocampal sclerosis. In the second part of this investigation, histologic specimens were analyzed to find an explanation for GWDL. METHODS: Retrospective visual assessment of hippocampal signal intensity and size and of ACWMp and GWDL was performed using 4- to 5-mm coronal T2-weighted spin-echo magnetic resonance images of 80 patients with histologically proven hippocampal sclerosis and of 30 age-matched controls without epilepsy. Frequency of occurrence and likelihood ratios of ACWMp and GWDL were calculated and their contribution to the diagnosis of hippocampal sclerosis was assessed, particularly in patients with no or restricted hippocampal abnormalities (either high signal or smaller size) on magnetic resonance imaging. The second part of the study involved the morphologic histologic assessment of neocortical temporal lobe specimens of all patients. Myelin density was evaluated in specimens of a subgroup of six patients with hippocampal sclerosis and GWDL on MRI and six patients with hippocampal sclerosis without GWDL. RESULTS: ACWMp was found in 68% and GWDL in 65% of patients with hippocampal sclerosis on magnetic resonance imaging. Both features had an infinite positive likelihood ratio. Sixty-two patients (77.5%) had concomitant hippocampal signal increase and smaller size. Eighteen patients (22.5%) had no or restricted hippocampal abnormalities on magnetic resonance imaging. When using ACWMp and GWDL as additional diagnostic parameters, 13 of these 18 patients were more unambiguously diagnosed as having hippocampal sclerosis. No significant morphologic differences were found between GWDL-positive and GWDL-negative specimens. A significantly lower average myelin stain was found in the white matter of the GWDL-positive group compared to the GWDL-negative group. CONCLUSIONS: ACWMp and GWDL can improve the visual diagnosis of hippocampal sclerosis, particularly in patients with no or restricted hippocampal abnormalities. These results suggest that loss of myelin may be the underlying cause of GWDL in association with hippocampal sclerosis.


Asunto(s)
Hipocampo/patología , Imagen por Resonancia Magnética , Lóbulo Temporal/patología , Adolescente , Adulto , Atrofia/diagnóstico , Atrofia/patología , Distribución de Chi-Cuadrado , Niño , Epilepsia del Lóbulo Temporal/diagnóstico , Epilepsia del Lóbulo Temporal/patología , Femenino , Humanos , Funciones de Verosimilitud , Imagen por Resonancia Magnética/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Esclerosis/diagnóstico , Esclerosis/patología , Sensibilidad y Especificidad , Estadísticas no Paramétricas
12.
Invest Radiol ; 32(5): 268-76, 1997 May.
Artículo en Inglés | MEDLINE | ID: mdl-9140746

RESUMEN

RATIONALE AND OBJECTIVES: Definition of optimal magnetic resonance (MR) scanning plane and conventional MR sequence for the detection of mesial temporal sclerosis (MTS). METHODS: Coronal and axial T2-weighted images and axial T2-weighted images parallel to the long axis of the hippocampus (APLAH) and coronal inversion recovery (IR) images were obtained in patients with medically intractable temporal lobe epilepsy in their phase 1 preoperative evaluation. Thirty-three consecutive MR scans were reviewed by a panel of three radiologists. Twenty-three patients had MR abnormalities consistent with MTS, and ten scans were normal. To assess the best single scanning technique, another group of three radiologists, who were masked to all patient data, individually assessed the different planes and sequences of the 33 studies presented separately in a random fashion. For each plane and sequence, the likelihood (L) ratio for the correct diagnosis was determined separately. RESULTS: For all planes considered separately, a likelihood ratio of 4.4 was optimal for the coronal T2-weighted images. The likelihood ratio of APLAH T2 was 2.2; of axial T2, 3.9; of coronal IR, indefinite because of 100% specificity. CONCLUSIONS: For the assessment of MTS, coronal T2-weighted images were considered the best single scanning technique.


Asunto(s)
Epilepsia del Lóbulo Temporal/patología , Imagen por Resonancia Magnética , Lóbulo Temporal/patología , Adolescente , Adulto , Epilepsia del Lóbulo Temporal/diagnóstico , Humanos , Funciones de Verosimilitud , Persona de Mediana Edad , Variaciones Dependientes del Observador , Esclerosis
13.
Brain Res ; 837(1-2): 55-66, 1999 Aug 07.
Artículo en Inglés | MEDLINE | ID: mdl-10433988

RESUMEN

To investigate epilepsy-associated changes in the presynaptic terminal, we isolated and characterized synaptosomes from biopsies resected during surgical treatment of drug-resistant temporal lobe epilepsy (TLE) patients. Our main findings are: (1) The yield of synaptosomal protein from biopsies of epilepsy patients was about 25% of that from rat brain. Synaptosomal preparations were essentially free of glial contaminations. (2) Synaptosomes from TLE patients and naive rat brain, quickly responded to K(+)-depolarization with a 70% increase in intrasynaptosomal Ca(2+) ([Ca(2+)](i)), and a 40% increase in B-50/GAP-43 phosphorylation. (3) Neocortical and hippocampal synaptosomes from TLE patients contained 20-50% of the glutamate and gamma-aminobutyric acid (GABA) contents of rat cortical synaptosomes. (4) Although the absolute amount of glutamate and GABA released under basal conditions from neocortical synaptosomes of TLE patients was lower than from rat synaptosomes, basal release expressed as percentage of total content was higher (16.4% and 17.3%, respectively) than in rat (11.5% and 9. 9%, respectively). (5) Depolarization-induced glutamate and GABA release from neocortical synaptosomes from TLE patients was smaller than from rat synaptosomes (3.9% and 13.0% vs. 21.9% and 25.0%, respectively). (6) Analysis of breakdown of glial fibrillary acid protein (GFAP) indicates that resection time (anoxic period during the operation) is a critical parameter for the quality of the synaptosomes. We conclude that highly pure and viable synaptosomes can be isolated even from highly sclerotic human epileptic tissue. Our data show that in studies on human synaptosomes it is of critical importance to distinguish methodological (i.e., resection time) from pathology-related abnormalities.


Asunto(s)
Epilepsia del Lóbulo Temporal/fisiopatología , Hipocampo/fisiopatología , Neocórtex/fisiopatología , Sinaptosomas/fisiología , Adulto , Animales , Neoplasias Encefálicas/patología , Neoplasias Encefálicas/fisiopatología , Neoplasias Encefálicas/cirugía , Calcio/metabolismo , Epilepsia del Lóbulo Temporal/patología , Epilepsia del Lóbulo Temporal/cirugía , Femenino , Proteína GAP-43/metabolismo , Proteína Ácida Fibrilar de la Glía/metabolismo , Ácido Glutámico/metabolismo , Hipocampo/patología , Hipocampo/cirugía , Humanos , Masculino , Potenciales de la Membrana/fisiología , Neocórtex/patología , Neocórtex/cirugía , Proteínas del Tejido Nervioso/metabolismo , Fosforilación , Potasio/farmacología , Ratas , Sinaptosomas/patología , Ácido gamma-Aminobutírico/metabolismo
14.
Clin Neurophysiol ; 110(2): 286-94, 1999 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-10210618

RESUMEN

OBJECTIVE: The purpose of the study was to make an objective and quantitative analysis of the EEG changes caused by carotid artery clamping during carotid endarterectomy (CEA) monitoring. METHODS: Factor analysis was used to study the intraoperative spectral EEG changes in 94 patients during clamping of the carotid artery. In addition, the relation between the extracted factors and the changes in blood pressure and blood flow velocity in the middle cerebral artery during clamping was studied. RESULTS: Two factors were extracted with factor analysis. The first factor represented a change in power in the alpha and beta frequency ranges in combination with a less pronounced opposite change in power in the delta frequency range. The second factor represented a change in power restricted to the delta and theta frequencies. With the first factor, two types of spectral EEG changes could be distinguished: changes indicative of cerebral ischemia (decrease in fast activity and increase in slow activity) and the opposite changes suggesting cerebral activation (arousal). With the two factors combined, the changes indicative of minor ischemia (decrease in fast activity only) could also be distinguished. CONCLUSION: Further study is required to test whether patients showing the EEG changes indicative of activation or minor ischemia actually require shunting.


Asunto(s)
Estenosis Carotídea/fisiopatología , Electroencefalografía , Endarterectomía Carotidea , Anciano , Análisis de Varianza , Mapeo Encefálico , Estenosis Carotídea/cirugía , Circulación Cerebrovascular/fisiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Monitoreo Fisiológico
15.
Clin Neurophysiol ; 111(8): 1505-13, 2000 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10904233

RESUMEN

OBJECTIVES: Besides preventing stroke, carotid endarterectomy (CEA) is reported to improve cerebral circulation and brain function. We tested whether this improvement is reflected by changes in the qEEG. METHODS: qEEG changes in 166 patients with a >70% stenosis of the internal carotid artery (ICA) were assessed after subtraction of the preoperative and postoperative spectra (eyes closed condition) before and 3 months after CEA. The mean frequency of the alpha band (MFA), the peak frequency of the alpha band (F alpha), and bands with limits relative to each patient's F alpha were studied in relation to neurological symptoms, patency or occlusion of the contralateral ICA, shunt requirement, and side of surgery. RESULTS: MFA and F alpha significantly increased over both hemispheres. After alignment on F alpha, a decrease of spectral band power was seen below F alpha, and a band power increase above F alpha. The group of patients with a contralateral ICA occlusion showed significantly more improvement than the group without. The group of patients with neurological deficits showed a tendency for improvement. No differences were found concerning shunt requirement or the side of surgery. CONCLUSIONS: After CEA the alpha rhythm frequency increases. In general, patients with a contralateral ICA occlusion improve more than other patients, in agreement with data from the literature on cerebral circulation and brain function.


Asunto(s)
Ritmo alfa , Isquemia Encefálica/fisiopatología , Encéfalo/fisiopatología , Endarterectomía Carotidea , Anciano , Anciano de 80 o más Años , Endarterectomía Carotidea/efectos adversos , Femenino , Humanos , Masculino , Persona de Mediana Edad
16.
Clin Neurophysiol ; 113(7): 1025-9, 2002 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-12088695

RESUMEN

OBJECTIVES: This study was performed to assess the extent of functional involvement of the affected hemisphere in Sturge Weber syndrome in comparison with the uninvolved hemisphere. To this end beta activity in the electroencephalogram (EEG) was measured, both before and after administration of diazepam intravenously (i.v.). METHODS: In 9 patients asymmetry in beta band activity was studied before and after diazepam administration. Several beta bands and asymmetry parameters were calculated. beta band asymmetries were compared with structural abnormalities (magnetic resonance imaging, MRI). RESULTS: Total beta activity was reduced in the involved hemisphere in all patients after diazepam administration. In 3 patients functional abnormalities were found in brain regions that were structurally intact. CONCLUSIONS: Decreased diazepam-enhanced beta activity in the EEG is a sensitive criterion of functional abnormality. In patients with subtle structural abnormalities diazepam-enhanced EEG may have added value in diagnosing functional involvement and in monitoring disease progression in patients.


Asunto(s)
Anticonvulsivantes , Ritmo beta/efectos de los fármacos , Diazepam , Imagen por Resonancia Magnética , Síndrome de Sturge-Weber/diagnóstico , Adolescente , Anticonvulsivantes/administración & dosificación , Encéfalo/patología , Niño , Preescolar , Diazepam/administración & dosificación , Femenino , Lateralidad Funcional/fisiología , Humanos , Lactante , Inyecciones Intravenosas , Masculino , Síndrome de Sturge-Weber/patología
17.
AJNR Am J Neuroradiol ; 15(8): 1547-55, 1994 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-7985576

RESUMEN

PURPOSE: To determine the frequency of appearance of various MR signs in mesial temporal sclerosis, to determine the optimal scanning planes for their visualization, and to propose a histologic explanation for the diminished demarcation between gray and white matter in the temporal lobe, a frequent MR finding in patients with mesial temporal sclerosis. METHODS: MR scans of 14 surgically treated patients with epilepsy and histologically proven mesial temporal sclerosis were assessed for the presence of six features: feature 1, high signal intensity in the hippocampus; 2, reduced hippocampal size; 3, ipsilateral atrophy of the hippocampal collateral white matter; 4, enlarged temporal horn; 5, reduced gray-white matter demarcation in the temporal lobe; and 6, decreased temporal lobe size. RESULTS: Feature 1 was present in 14 patients and was best appreciated on the T2-weighted images in planes parallel to the long axes of the hippocampi. Feature 2, present in 12 patients, and feature 6, present in 9 patients, were optimally seen in the coronal planes and on the inversion-recovery sequences in particular. Feature 3, present in 12 patients, was optimally seen on the coronal T2-weighted images. Feature 4, seen in 11 patients, was equally well seen in all planes (transverse, coronal, and parallel to the long axes of the hippocampi). Feature 5, seen in 10 patients, was best appreciated on the T2-weighted images in the planes of the long axes of the hippocampi. Histologic investigation of the temporal lobe white matter in the 10 patients with feature 5 demonstrated on the MR scan showed abnormalities in 7 cases. Oligodendroglia cell clusters were found in 6, with concomitant corpora amylacea in 1 case and perivascular macrophages with pigment a sole finding in another case. CONCLUSION: Of the six features found in cases of mesial temporal sclerosis on MR, increased hippocampal signal intensity is the most consistent. A decreased gray-white matter demarcation in the temporal lobe parenchyma is also a frequent feature of this disease. A combination of multiple scanning planes results in an optimal demonstration of lesions.


Asunto(s)
Epilepsia del Lóbulo Temporal/patología , Imagen por Resonancia Magnética , Lóbulo Temporal/patología , Adolescente , Adulto , Amiloide , Atrofia , Femenino , Gliosis/patología , Hipocampo/patología , Humanos , Hipertrofia , Aumento de la Imagen/métodos , Macrófagos/patología , Imagen por Resonancia Magnética/métodos , Masculino , Oligodendroglía/patología , Estudios Retrospectivos , Esclerosis , Lóbulo Temporal/irrigación sanguínea
18.
AJNR Am J Neuroradiol ; 17(10): 1815-9, 1996.
Artículo en Inglés | MEDLINE | ID: mdl-8933863

RESUMEN

A three-dimensional fast spin-echo MR technique is proposed for locating contact points on implanted intracerebral multicontact electrode bundles, Coronal or sagittal reformatting shows the entire trajectory of the electrode bundles. The contract points are clearly visible owing to the absence of coating material associated with a slightly larger susceptibility artifact. Potentially, this technique may preclude postimplantation thin-section CT, with its associated high radiation dose.


Asunto(s)
Encéfalo/patología , Electrodos Implantados , Epilepsia/patología , Imagen por Resonancia Magnética/métodos , Acero Inoxidable , Artefactos , Epilepsia/cirugía , Humanos , Procesamiento de Imagen Asistido por Computador , Fantasmas de Imagen , Técnicas Estereotáxicas
19.
J Neurol Sci ; 110(1-2): 99-106, 1992 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-1506876

RESUMEN

In the experimental rat model of diabetes a slowing of nerve conduction velocity and a resistance to ischemic conduction failure have been found as an indication of polyneuropathy. The same electrophysiological abnormalities have been demonstrated in a model in which healthy rats are kept under hypoxic conditions (10% O2) for a 10-week period. Two factors are held responsible for the development of diabetic polyneuropathy: metabolic deterioration and hypoxia. However, until now the relative roles of metabolic deterioration and hypoxia in the development of polyneuropathy have not been settled. To test both explanations further with more sophisticated electrophysiological techniques, the H-reflex (motor and sensory NVC) and the stimulated SF-EMG (measures terminal nerve branch and neuromuscular transmission) were measured in 3 groups of 10 rats, a healthy control group, a diabetic group, and a hypoxic group, every 5 weeks, for 6 months. In the control rats an age-related increase in motor and sensory conduction velocity was found, whereas in the diabetic rats as well as in the hypoxic rats a marked decrease in sensory and a slight decrease in motor nerve conduction velocity was observed. The jitter measured in the stimulated SF-EMG was significantly increased in both the diabetic and the hypoxic group. The results of the present study support the possible role of hypoxia, in addition to metabolic factors, in the development of experimental diabetic neuropathy.


Asunto(s)
Diabetes Mellitus Experimental/fisiopatología , Neuropatías Diabéticas/fisiopatología , Ganglios Espinales/fisiopatología , Isquemia/fisiopatología , Neuronas Motoras/fisiología , Enfermedades del Sistema Nervioso/fisiopatología , Conducción Nerviosa , Nervio Ciático/fisiopatología , Vías Aferentes/fisiología , Vías Aferentes/fisiopatología , Animales , Glucemia/metabolismo , Electromiografía , Ganglios Espinales/fisiología , Hemoglobinas/análisis , Hipoxia/fisiopatología , Masculino , Ratas , Ratas Endogámicas , Valores de Referencia , Nervio Ciático/fisiología
20.
Neurosurgery ; 26(1): 93-101, 1990 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-2294484

RESUMEN

For intracranial recording of partial seizures considered to originate from one of the temporal or frontal lobes, the team in the Utrecht Academic Hospital has used subdural, multicontact, flexible electrodes since 1972. These are introduced through bilateral, frontocentral trephine holes and are manipulated under fluoroscopy to cover most of the cerebral convexity. It became evident that in many patients, additional placements to record from intracerebral structures were indispensable. Therefore, using the same trephine holes, an additional 2 to 4 depth electrodes were stereotactically implanted in the mesial temporal and/or frontal structures, as appropriate. An extensive intra- and extracerebral spatial representation of the epileptogenic zone was thus obtained. We report here the methods for manufacturing and applying these electrodes and our clinical experience with 28 patients. The results obtained so far stress the value of combining subdural and depth electroencephalographic monitoring in the presurgical selection of patients suffering from medically refractory complex partial seizures. By miniaturizing the electrodes, extensive areas of the brain can be investigated without craniotomy or multiple burr holes.


Asunto(s)
Electrodos , Electroencefalografía/métodos , Epilepsia/diagnóstico , Adulto , Encéfalo/fisiopatología , Epilepsia/fisiopatología , Femenino , Humanos , Masculino , Cuidados Preoperatorios , Espacio Subdural
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