Bio-based biodegradable film to replace the standard polyethylene cover for silage conservation.

The research was aimed at studying whether the polyethylene (PE) film currently used to cover maize silage could be replaced with bio-based biodegradable films, and at determining the effects on the fermentative and microbiological quality of the resulting silages in laboratory silo conditions. Biodegradable plastic film made in 2 different formulations, MB1 and MB2, was compared with a conventional 120-µm-thick PE film. A whole maize crop was chopped; ensiled in MB1, MB2, and PE plastic bags, 12.5kg of fresh weight per bag; and opened after 170d of conservation. At silo opening, the microbial and fermentative quality of the silage was analyzed in the uppermost layer (0 to 50mm from the surface) and in the whole mass of the silo. All the silages were well fermented with little differences in fermentative quality between the treatments, although differences in the mold count and aerobic stability were observed in trial 1 for the MB1 silage. These results have shown the possibility of successfully developing a biodegradable cover for silage for up to 6mo after ensiling. The MB2 film allowed a good silage quality to be obtained even in the uppermost part of the silage close to the plastic film up to 170d of conservation, with similar results to those obtained with the PE film. The promising results of this experiment indicate that the development of new degradable materials to cover silage till 6mo after ensiling could be possible.

Effect of applying inoculants with heterolactic or homolactic and heterolactic bacteria on the fermentation and quality of corn silage.

This study examined the effect of applying different bacterial inoculants on the fermentation and quality of corn silage. Corn plants were harvested at 35% DM, chopped, and ensiled in 20-L mini silos after application of (1) deionized water (CON) or inoculants containing (2) 1 × 10(5) cfu/g of Pediococcus pentosaceus 12455 and Propionibacteria freudenreichii (B2); (3) 4 × 10(5) cfu/g of Lactobacillus buchneri 40788 (BUC); or (4) 1 × 10(5) cfu/g of Pediococcus pentosaceus 12455 and 4 × 10(5) cfu/g of L. buchneri 40788 (B500). Four replicates of each treatment were weighed into polyethylene bags within 20-L mini silos. Silos were stored for 575 d at ambient temperature (25°C) in a covered barn. After silos were opened, aerobic stability, chemical composition, and yeast and mold counts were determined. The DNA in treated and untreated silages was extracted using lysozyme/sodium dodecyl sulfate lysis and phenol/chloroform and used as a template for a conventional PCR with primers designed on the 16S rRNA gene to detect the presence of L. buchneri in all silage samples. Acetic acid concentration was greater in B2 silages versus others (6.46 vs. 4.23% DM). Silages treated with BUC and B500 had lower pH and propionic acid concentration and greater lactic acid concentration than others. The B500 silage had the greatest lactic:acetic acid ratio (1.54 vs. 0.41), and only treatment with BUC reduced DM losses (5.0 vs. 14.3%). Yeast and mold counts were less than the threshold (10(5)) typically associated with silage spoilage and did not differ among treatments. Consequently, all silages were very stable (>250 h). Aerobic stability was not improved by any inoculant but was lower in B500 silages versus others (276 vs. 386 h). The conventional PCR confirmed the presence of similar populations of L. buchneri in all silages. This may have contributed to the prolonged aerobic stability of all silages.

[Medical and biological safety assessment of genetically modified maize strain MIR604].

The results of toxicologo-hygienic examinations, which were conducted within the framework of integrated medical and biological assessment of genetically modified rootworm Diabrotica spp.-protected maize event MIR604, are presented. Analysis of morphological, hematological, biochemical parameters and system (sensitive) biomarkers has not confirmed any toxic effect of maize event MIR604.

[Medical and biological safety assessment of genetically modified maize event MIR604].

There are presented the results of genotoxicologic, immunologic and allergologic examinations which were conducted within the framework of integrated medical and biological assessment of genetically modified rootworm Diabrotica spp.-protected maize event MIR604. Analysis of damages of DNA and structural chromosome aberrations, assessment of the allergenic potential and immunoreactive properties has not confirmed any genotoxic, allergenic and immunotoxic effect of maize event MIR604.

Relationship between structural and biochemical characteristics and texture of corn grains.

The texture of corn grains is a fundamental characteristic for the industry as well as for grain producers and processors. To further understand the mechanisms involved in grain hardness, contrasting corn cultivars for grain hardness and protein quality were evaluated. The cultivars were Cateto L237/67 (hard endosperm and low protein value), QPM BR 451 (semi-hard endosperm and high protein value); Bolivia-2 (floury endosperm and low protein value), and Opaque-2 (floury endosperm and high protein value). Evaluations were carried out at 10, 20, 30, 40, 50, and 60 days after pollination in developing corn grains and in the mature grain. In developing grains, evaluation consisted in the determination of the area, percentage of starch granules, distribution of starch granules, and protein bodies in the endosperm. In mature corn grains, the parameters evaluated were: density, percentage of total proteins, levels of lysine and tryptophan, and banding pattern of zeins. The results indicate that the higher physical resistance of corn grains from the cultivars analyzed is influenced by the high percentage of total proteins, high synthesis of 27-kDa zeins, presence of protein bodies, and perfect organization of starch granules in the endosperm, independent of their sizes.

Determination of fumonisins B1 and B2 in corn by liquid chromatography/mass spectrometry with immunoaffinity column cleanup: single-laboratory method validation.

A single-laboratory method validation was conducted to establish the effectiveness of an immunoaffinity column cleanup procedure followed by liquid chromatography/mass spectrometry (LCIMS) for the determination of fumonisins B1 and B2 (FBI + FB2) in corn. The test portion is extracted with acetonitrile-methanol-water (25 + 25 + 50). The extract is filtered, diluted with phosphate-buffered saline solution, and applied to an immunoaffinity column. FB1 + FB2 are removed with methanol and directly determined by reversed-phase LC with MS detection using selected-ion monitoring of 2 characteristic ions in each case. Test portions of blank corn samples were spiked with a mixture of FB1 + FB2 to give total levels of 200 and 500 ng/g, respectively. Recoveries of both FB1 and FB2 from spiked samples averaged 90.4-101%. Based on results for spiked raw corn (triplicates at 2 levels), the relative standard deviation for repeatability ranged from 2.8 to 7.1%. The accuracy of the method was demonstrated by analysis of Food Analysis Performance Assessment Scheme (FAPAS) test material. The method was also applied to a small survey of processed corn products such as corn chips, cornflakes, and popcorn.

[The effect of consuming quality protein maize or conventional maize on the growth and morbidity of malnourished Nicaraguan children 1 to 5 years of age]. / Efectos de la ingesta de maíz de alta calidad de proteína (QPM) versus maíz convencional en el crecimiento y la morbilidad de niños nicaragüenses desnutridos de 1 a 5 años de edad.

Quality protein maize (QPM), with twice the amount of tryptophan and lysine than conventional maize, has improved the nutritional status of severely malnourished children. This double-blind clinical study evaluated the impact of QPM on the growth and morbidity of mild and moderately malnourished children. In a Nicaraguan day care center, 48 children 1 to 5 years old who were malnourished (> 2 indicators with < -1 Z for weight-age, height-age or weight-height) were identified and randomly assigned to consume for 5 days/week for 3.5 months a snack prepared with QPM or conventional maize. QPM positively influenced children's growth: weight (0.80 vs. 0.19 kg gained from baseline to endline between the QPM and conventional maize groups, respectively), height (2.02 vs. 1.23 cm in QPM vs. conventional) and Z score for weight-age (0.17 vs. -0.26 Z in QPM vs. conventional) and height-age (0.06 vs. -0.23 Z in QPM vs. conventional). When other factors that could affect growth with respect to weight, height, weight-age Z score and height-age Z score were controlled for, the intervention group (QPM > conventional) was a statistically important factor (P < 0.01). The QPM snack, however, had no effect on the incidence of diarrheal episodes or respiratory infections. In conclusion, QPM improves the nutritional status of pre-school children who are mild or moderately malnourished but has no effect on the incidence of diarrheal episodes or respiratory infections.

Effects of ionizing radiation on sensorial, chemical, and microbiological quality of frozen corn and peas.

The effects of irradiation (0, 1.8, and 4.5 kGy) on the quality of frozen corn and peas were investigated during a 12month period of postirradiation storage at -18 degrees C. Irradiation of frozen corn and peas caused a reduction in ascorbic acid content of both vegetables and a loss of texture in peas but had no significant effects on instrumental color parameters (L*, a*, and b*), carotenoid and chlorophyll content, or antioxidant capacity of corn and peas. Irradiation reduced microbial loads of frozen peas and increased display life at 23 degrees C of thawed peas by preserving the green color, apparently because of slower increases in the population of acid-producing microorganisms in the irradiated samples. Overall, irradiation significantly reduced the microbial load and increased the display life of peas and had minimal detrimental effects on the quality of frozen corn and peas.

Preexercise meal composition alters plasma large neutral amino acid responses during exercise and recovery.

This study was designed to determine the effect of ingesting three preexercise meals on energy metabolism during exercise and recovery and to relate metabolic perturbations to subjective and objective measurements associated with central fatigue. Twelve subjects consumed isoenergetic meals consisting of oat, wheat, or corn cereals 90 min before cycling. A fasting trial served as the control. Blood samples and cognitive function, perceived hunger, and sleepiness measurements were obtained before and after feeding and during recovery when self-selected food intake was also measured. After meal ingestion, plasma insulin was lower for oat than for wheat or corn whereas the ratio of tryptophan to large neutral amino acids (LNAAs) for corn was less than for all others. During exercise, the tryptophan-LNAA ratio increased from preexercise values for the fasting and wheat trials, but exercise performance was unaffected. During recovery, tryptophan:LNAA increased from postexercise values in fasting trials. Also, hunger and fatigue ratings were greater in fasted subjects, but self-selected food intake measured at the end of the recovery period was not different among groups. We conclude that preexercise meal consumption affected tryptophan:LNAA before, during, and after exercise, but these changes were not sufficient to alter physical and cognitive performance.

The influence of different cereal grains on iron absorption from infant cereal foods.

Iron absorption from various cereal grains was evaluated in the present study to identify possible preferences for the preparation of infant weaning foods. In six separate studies, four radioiron absorption tests were performed in each of 57 volunteer subjects by using a sequential double-isotopic method. Serum ferritin concentration was used to adjust for the effect of differences in the iron status of subjects participating in separate studies. Identical commercial processing and test meal composition were used to evaluate iron absorption from 50 g cooked cereal prepared from rice, wheat, maize, oats, millet, and sweet or bitter quinoa. In an initial evaluation of cereals fortified with 2.5 mg Fe as FeSO4, geometric mean absorption values were uniformly < 1% for all cereals and were not significantly different. In subsequent studies, percentage iron absorption was enhanced by either eliminating the fortifying iron or adding 50 mg ascorbic acid to the test meal. The effect was similar for most of the cereals tested with a composite mean increase in absorption of 37% when fortifying iron was removed and 270% when ascorbic acid was added. There was a strong inverse correlation between iron absorption and the phytate content of different cereals. Except for a modestly lower absorption of iron from quinoa and a remarkably higher absorption from one lot of maize, we conclude that the type of cereal grain has little influence on iron bioavailability of infant cereals. On the other hand, modification in the milling and processing methods for cereal grains that reduce their content of phytic acid is likely to improve iron availability significantly.

Nutrition disorders and immunologic parameters: study of the thymus in growing rats.

OBJECTIVES: We studied the effect of a low-quality dietary protein on cellular proliferation and maturation in the thymus of growing rats over time. METHODS: After weaning Wistar rats were fed a diet containing 6.5 g/100 g of corn flour for 6, 10, 18, and 45 d (M groups). For comparison, other rats were fed a diet containing 6.5 g/100 g of casein (Cas groups), and well-nourished age-matched control rats were fed a commercial laboratory diet (C groups). Food intake, body weight, thymus weight, total number of thymocytes, and the percentages of CD43(+) and Thy1(+) thymocyte phenotypic antigen determinants were measured. RESULTS: M versus Cas and C groups showed significant differences (P < 0.01) in body and thymus weights after 6 d of feeding, and the total number of thymocytes and the percentages of CD43(+) and Thy1(+) were significantly lower after 10 d of feeding. The results indicated that consuming a cereal diet for short or long periods causes thymus atrophy in growing rats, with significant reductions in the total number of T-cells concomitant with increases in the number of immature thymocytes. CONCLUSIONS: The data showed that, in addition to low-protein concentration, low-quality dietary protein is a limiting factor in certain steps of cellular intrathymic pathways, probably related to the requirement of specific amino acids for optimal immune response.

Changes in availability and nutritive value of different corn residue parts as affected by early and late grazing seasons.

Two early- (EG) and two late-season (LG) corn-residue grazing trials were conducted either from October to December or from December to March (1987 and 1988) using both nonirrigated and irrigated fields. Initial availability, disappearance, and nutritive value of different corn residue components were estimated. Stocking rates were 1.97, 2.20, 1.36, and 2.58 animals/ha for the two EG and LG nonirrigated fields (NIF), respectively, and 3.96 animals/ha for EG irrigated fields (IF). Samples were collected three times throughout the grazing period. In one of the LG trials, samples were collected 30 d before the grazing season began for measurement of weathering damage. Composite samples were analyzed for CP, ADF, ADIN, and in vitro DM (IVDMD) and OM (IVOMD) disappearance. Ruminal escape protein (EP) content was assessed using a Dacron bag technique. Weathering reduced (P less than .02) the amount of leaf blade available by 42% during a period of 30 d before the LG season in Trial 2. Grain, husks, and leaf blades disappeared to the greatest degree (P less than .05) during either grazing season. More than 90% of the grain disappeared during the first 30 d, regardless of grazing season. Overall IVDMD for NIF grain, husks, leaf blades, stems, and cobs across the EG and LG trials were 98.6, 68.4, 42.0, 41.0, and 48.1%, respectively. Corn residue fractions from IF had a lower (P less than .05) IVDMD than did NIF fractions. Grain from NIF was 20% higher (DM basis) in EP than grain from IF (Trial 2). Grain left in the field was the most important source of CP and EP for calves grazing corn residues. Husks and leaf blades were selected by calves; husks were the roughage fraction with the highest nutritive value.

Effects of particle size and pelleting on growth performance, nutrient digestibility, and stomach morphology in finishing pigs.

The effects of particle size and pelleting on growth performance, carcass characteristics, nutrient digestibility, and stomach morphology were determined using 160 finishing pigs. The pigs were fed a corn-soybean meal-based diet with the corn milled to particle sizes of 1,000, 800, 600, or 400 microns. The diets were fed in meal or pellet form. Pelleting the diets resulted in 5% greater ADG (P < .01) and 7% greater grain/feed (P < .001). Also, pelleting increased digestibilities of DM, N, and GE by 5 to 8% (P < .001). Reducing particle size increased electrical energy required for milling and decreased milling production rates, especially as particle size was decreased from 600 to 400 microns. Reducing particle size of the corn from 1,000 to 400 microns increased gain/feed by 8% (linear effect, P < .001) and digestibility of GE by 7% (quadratic effect, P < .03). Improved nutrient digestibility and lower ADFI resulted in 26% less daily excretion of DM and 27% less daily excretion of N in the feces as particle size was reduced from 1,000 to 400 microns (linear effects, P < .001). Stomach lesions and keratinization increased with reduced particle size (P < .003) and keratinization increased with pelleting (P < .02), although they were unrelated to growth performance (i.e., gain/feed actually improved as lesion scores increased). Considering milling energy, growth performance, stomach morphology, nutrient digestibility, and nutrient excretion, a particle size of 600 microns, or slightly less, is an acceptable compromise for corn in both meal and pelleted diets for finishing pigs.

Determining the net energy value of wet and dry corn gluten feed in beef growing and finishing diets.

One growing and two finishing trials evaluated the feeding value of corn gluten feed for cattle. In the growth trial, calves were fed (DM basis) one of two control diets (44% dry-rolled corn [DRC], 50% alfalfa hay, 5% molasses, 1% supplement or 33% DRC, 33% alfalfa hay, 33% corn silage, 1% supplement), diets containing 49 or 65% wet corn gluten feed (WCGF) with alfalfa hay and supplement, or a diet containing 61% WCGF with cornstalks and supplement. Calves fed diets containing 49 or 65% WCGF with alfalfa hay gained faster (P < .10) and more efficiently (P < .10) than calves fed the two control diets. In finishing trial 1, yearling steers were fed 92.5% concentrate diets containing (DM basis) 79% DRC, a combination of DRC and 35 or 70% WCGF, a combination of DRC and 70% dry corn gluten feed (DCGF) with or without added water, or a combination of high-moisture corn (HMC) and 70% WCGF. Steers fed 35 or 70% WCGF in combination with either DRC or HMC had gains and efficiencies similar (P > .10) to those of steers fed DRC. Inclusion of water in the 70% DCGF diet reduced DMI (P < .10) and daily gain (P < .10) but did not affect (P > .10) feed efficiency. In Trial 2, WCGF replaced either 20, 40, 60, 80, or 100% of the DRC and molasses (DM basis). Feed efficiency was not different (P > .10) among diets.(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of protein concentration and protein source on performance of newly arrived feedlot steers.

In Trial 1, 240 crossbred steers (initial BW 243 +/- 8 kg) were used in a 2 x 4 factorial experiment to determine the effects of receiving diet CP concentration (12, 14, 16, or 18%) and source (spray-dried blood meal [SDBM] vs soybean meal [SBM]) on steer performance. There were linear (P < .01) increases in ADG and feed efficiency during wk 1. For the entire trial, there was an increase (P < .01) in feed efficiency with increasing CP concentration and diets containing SDBM compared with those containing SBM. In Trial 2, 240 crossbred steers (initial BW 246 +/- 14 kg) were used in a completely randomized design experiment to determine the effects of receiving diet CP concentration (11, 14, 17, 20, 23, or 26%) on steer performance. Average daily gain and feed efficiency increased (P < .01) with increasing CP concentration during wk 1. There were quadratic (P < .01) responses to CP concentration for final weight, ADG, and feed efficiency. In Trial 3, 216 steers (initial BW 238 +/- 1 kg) were used to determine the effects of receiving diet protein sources on steer performance. The control diet used SBM as the supplemental CP source and was formulated to contain 12.5% CP. The other five protein sources were corn gluten meal (CGM), ring-dried blood meal (RDBM), SDBM, fish meal (FM), and SBM. For these five CP sources, diets were formulated to contain 23% CP during wk 1, 17% CP during wk 2, and 12.5% CP during wk 3 and wk 4. During wk 1, control steers had the lowest ADG (P < .07) and feed efficiency (P < .01) whereas steers fed RDBM and SDBM had the highest (P < .01) feed efficiency. Increased CP concentrations are needed early in the receiving period, when DMI is low.

Dust suppression in swine feed using soybean oil.

Dust generation from swine feed (corn-soybean meal diet) treated with soybean oil was investigated using a cement mixer in a plywood box. Airborne total dust concentrations in the box were measured gravimetrically using a vacuum pump and filters while 12-kg feed samples were constantly mixed. The treatment factors were soybean oil concentration (0, 1, and 3%), corn bulk density (normal, 730 kg/m3 and low, 600 kg/m3), time of oil addition (before vs after grinding the corn), and storage time (0, 7, and 14 d). The feed was a mixture of soybean meal, base mix, and adjusted amounts of ground corn and soybean oil. Adding soybean oil after grinding at 1 and 3% levels to feed made with normal-bulk density corn suppressed total dust generation (3.39 and .99 mg/m3, respectively) (P < .001) compared with the no oil treatment (29.1 mg/m3). The 3% soybean oil treatment suppressed dust generation (.99 mg/m3) more than the 1% soybean oil treatment (3.39 mg/m3) (P < .001). Adding soybean oil after grinding the corn suppressed dust generation more than adding the oil to the corn before grinding for every oil level (P < .001). More dust was generated by feed made from low-bulk density corn than by feed made with normal-bulk density corn at every oil level (P < .001). There was no evidence of any storage time or treatment x storage time interactions.

Performance and carcass characteristics of weaned lambs either grazed on orchardgrass, ryegrass, or alfalfa or fed all-concentrate diets in drylot.

Four-paddock rotational grazing of orchardgrass, ryegrass, or alfalfa was compared to an all-concentrate diet fed in drylot using 202 weaned lambs. Comparisons of animal performance ended in each of the 3 yr (1983, 1984, 1985) for all animals when drylot lambs reached a subjective body condition score of 12 (range 1 to 15) and estimated fat depth over the rib was 5.5 to 7.0 mm. A total of 84 representative lambs were slaughtered over the 3 yr for carcass evaluation. Mature put-and-take ewes were used to maintain forages in the vegetative stage. Average daily gain and total gain of lambs were in the order of drylot > alfalfa > grasses; performance of lambs grazing alfalfa approached performance of those fed the drylot diet. Better performance (P < .01) of lambs grazing alfalfa than of those grazing grasses is atributed to more CP (P < .01) and less NDF, ADF, and hemicellulose (P < .01) in alfalfa. Lambs grazed on grasses had smaller carcasses with less muscle, fat, and bone (P < .01) than either of the other two treatments. Although carcasses of lambs grazed on alfalfa were lighter, they had the same muscle mass as concentrate-fed lambs, indicated by leg conformation, longissimus muscle area, and by physically separated absolute muscle weight. However, these carcasses contained less fat (P < .01) and had more desirable yield grades (P < .01) than the carcasses of concentrate-fed lambs.

Wet corn distillers byproducts compared with dried corn distillers grains with solubles as a source of protein and energy for ruminants.

Five trials investigated the feeding value of wet and dried corn distillers byproducts as a source of protein and energy for growing and finishing cattle and investigated the effect of heat damage on the feeding value of dried distillers byproducts. In a calf growth trial, no differences in rate of gain or protein efficiency were observed among calves fed wet distillers byproducts (wet distillers grains + thin stillage; WDB) or one of three composites of dried distillers grain + solubles (DDGS) having a low, medium, or high concentration of ADIN. A finishing trial compared the energy value of dry-rolled corn (DRC) with WDB or the three DDGS composites, fed at 40% of the diet DM replacing DRC. Cattle consuming WDB or DDGS gained faster (P < .05) and more efficiently (P < .05) than cattle fed DRC. Although gains were similar, cattle fed WDB consumed less feed (P < .10) and were more efficient (P < .10) than cattle fed DDGS. Level of ADIN in DDGS did not affect efficiency of gain (P > .10). In a lamb finishing trial, the addition of 5 or 10% ethanol did not affect (P > .10) daily gain, DMI, or feed efficiency. In two metabolism trials with steers, grain byproducts (wet distillers grains, dry distillers grains plus solubles, wet corn gluten feed, dry corn gluten feed, hominy feed) and DRC had similar effects on ruminal pH and total VFA. Feeding thin stillage or condensed solubles reduced (P < .10) ruminal pH and tended to reduce acetate:propionate. Wet corn distillers byproducts, fed at 40% of the diet DM, contain more NEg than did DRC and drying WDB reduces its NEg content. Acid detergent insoluble N is a poor indicator of protein and energy value in distillers grains.