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Effect of phenylmethylsulfonyl fluoride, a protease inhibitor, on enamel surface remineralization

Peres, Paulo Edelvar Corrêa; Fu, Jean; Zero, Domenick T; Cury, Jaime Aparecido.
Braz. j. oral sci ; 22: e230883, Jan.-Dec. 2023. ilus
Artículo en Inglés | LILACS, BBO - odontología (Brasil) | ID: biblio-1399769
Phenylmethylsulfonyl fluoride (PMSF) is a protease inhibitor widely used in research, but fluoride is released during its action and this knowledge has been neglected in dental research.

Aim:

to evaluate if fluoride released by salivary protease action on PMSF affects enamel remineralization and fluoride uptake.

Methods:

Groups of 10 enamel slabs, with caries-like lesions and known surface hardness (SH), were subjected to one of the following treatment groups Stimulated human saliva (SHS), negative control; SHS containing 1.0 µg F/mL (NaF), positive control; and SHS containing 10, 50 or 100 µM PMSF. The slabs were subjected to a pH-cycling regimen consisting of 22 h/day in each treatment solution and 2 h/day in a demineralizing solution. After 12 days, SH was again measured to calculate the percentage of surface hardness recovery (%SHR), followed by enamel fluoride uptake determination. The time-related fluoride release from 100.0 µM PMSF by SHS action was also determined. Data were analyzed by ANOVA followed by Newman-Keuls test.

Results:

The release of fluoride from PMSF by SHS was rapid, reaching a maximum value after 10 min. Fluoride released from PMSF was more effective in enhancing %SHR and increasing fluoride uptake in enamel compared with SHS alone (p < 0.05); furthermore, it was equivalent to the positive control (p > 0.05).

Conclusion:

In conclusion, fluoride released by saliva from PMSF is available to react with enamel and needs to be taken into account in research using this protease inhibitor
Biblioteca responsable: BR218.1