Epitopes targeted by bullous pemphigoid T lymphocytes and autoantibodies map to the same sites on the bullous pemphigoid 180 ectodomain.
J Invest Dermatol
; 115(6): 955-61, 2000 Dec.
Article
en En
| MEDLINE
| ID: mdl-11121125
ABSTRACT
Bullous pemphigoid is a blistering skin disease characterized by autoantibodies directed against the NC16A domain of bullous pemphigoid 180 (collagen XVII), a transmembrane protein of epidermal basal cells. Passive transfer studies in mice have shown that antibodies that bind to this immunodominant region of bullous pemphigoid 180 are capable of inducing a skin disease that closely mimics bullous pemphigoid, supporting the hypothesis that epitopes within NC16A are involved in the pathogenesis of bullous pemphigoid. In this study, we examined the autoimmune T cell response in bullous pemphigoid patients. T cells from eight of 12 bullous pemphigoid patients, all of whom had circulating anti-bullous pemphigoid 180 autoantibodies, showed a specific proliferative response to recombinant forms of NC16A. T cell lines and clones developed from four of these patients recognize the same NC16A peptides as those targeted by autoantibodies from the corresponding individuals. These NC16A-responding T lymphocytes express alpha/beta T cell receptors and CD4 memory T cell surface markers and exhibited a Th1/Th2 mixed cytokine profile that may support the production of antibodies. This new information will aid in defining the key steps involved in the development of the autoimmune response in bullous pemphigoid.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Penfigoide Ampolloso
/
Proteínas del Citoesqueleto
/
Colágenos no Fibrilares
/
Proteínas del Tejido Nervioso
Límite:
Humans
Idioma:
En
Revista:
J Invest Dermatol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos