Physical and transcript map of the hereditary prostate cancer region at xq27.
Genomics
; 79(1): 41-50, 2002 Jan.
Article
en En
| MEDLINE
| ID: mdl-11827456
ABSTRACT
We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25-q27) through a genome-wide linkage study. Here we report the construction of an approximately 9-Mb sequence-ready bacterial clone contig map of Xq26.3-q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at approximately 85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3-q27.3.
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Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
/
Cromosoma X
/
Mapeo Cromosómico
Tipo de estudio:
Etiology_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos