Regulation of the pollen-specific actin-depolymerizing factor LlADF1.
Plant Cell
; 14(11): 2915-27, 2002 Nov.
Article
en En
| MEDLINE
| ID: mdl-12417710
ABSTRACT
Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the lily pollen-specific actin-depolymerizing factor (ADF) LlADF1. Its actin binding and depolymerizing activity is pH sensitive, inhibited by certain phosphoinositides, but not controlled by phosphorylation. Compared with its F-actin binding properties, its low activity in depolymerization assays has been used to explain why pollen ADF decorates F-actin in pollen grains. This low activity is incompatible with a role in increasing actin dynamics necessary to promote pollen tube growth. We have identified a plant homolog of actin-interacting protein, AIP1, which enhances the depolymerization of F-actin in the presence of LlADF1 by approximately 60%. Both pollen ADF and pollen AIP1 bind F-actin in pollen grains but are mainly cytoplasmic in pollen tubes. Our results suggest that together these proteins remodel actin filaments as pollen grains enter and exit dormancy.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polen
/
Actinas
/
Magnoliopsida
/
Proteínas de Microfilamentos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Plant Cell
Asunto de la revista:
BOTANICA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Reino Unido