Patch-clamp experiments with porins extracted from a marine bacterium (Photobacterium profundum strain SS9) and reconstituted in liposomes.
Cell Biochem Biophys
; 37(3): 157-67, 2003.
Article
en En
| MEDLINE
| ID: mdl-12625625
ABSTRACT
The reconstitution of bacterial porins in liposome bilayers for patch-clamp recording is well established. However, the solutions used in the dehydration, rehydration, and osmotic swelling of the liposomes have been developed for porins from enteric bacteria. Porins from marine bacteria normally function in contact with seawater whose ionic composition and osmotic pressure would appear to be incompatible with the established methods. Here, we show that, contrary to expectation, an established reconstitution and patch-clamp method works well with porins, mainly OmpH and OmpL, extracted from the deep-sea marine bacterium Photobacterium profundum strain SS9 and that seawater can be introduced at a supplementary stage.
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Banco de datos:
MEDLINE
Asunto principal:
Photobacterium
/
Proteínas Bacterianas
/
Porinas
/
Técnicas de Placa-Clamp
/
Proteínas de Escherichia coli
/
Liposomas
Idioma:
En
Revista:
Cell Biochem Biophys
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Reino Unido