PEG-inhibitor conjugates: application of diphenyl alpha-aminoalkylphosphonate to removal of chymotrypsin.
Biosci Biotechnol Biochem
; 67(10): 2273-6, 2003 Oct.
Article
en En
| MEDLINE
| ID: mdl-14586120
ABSTRACT
Diphenyl 1-amino-2-phenylethylphosphonate was introduced to poly(ethylene glycol)s (PEGs) with average molecular masses of 300, 400, and 600 to prepare water-insoluble PEG-inhibitor conjugates. Interestingly, only the conjugate from PEG with an average molecular weight of 600 formed a precipitate with chymotrypsin but not with trypsin. The results demonstrated that the PEG-inhibitor conjugate is useful for separation of chymotrypsin.
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Banco de datos:
MEDLINE
Asunto principal:
Polietilenglicoles
/
Inhibidores de Proteasas
/
Quimotripsina
/
Organofosfonatos
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón