Screening the whole genome of a pathogen in vivo for individual protective antigens.
Vaccine
; 23(23): 3016-25, 2005 Apr 27.
Article
en En
| MEDLINE
| ID: mdl-15811648
ABSTRACT
We report the results of a general protocol that was used to screen the whole genome of Chlamydophila abortus, type strain B577 (formerly Chlamydia psittaci strain B577), in a mouse pneumonia model. Genetic immunization was used to functionally test the genes of C. abortus as vaccines in a mouse challenge system. Nine gene fragments were isolated that conferred protection, with five protecting as effectively as the live-vaccine positive control. Bioinformatics approaches were unable to reconstruct isolation of these antigens. These results suggest that pathogen genomes can be functionally screened for vaccine candidate antigens in a mouse model to reveal new classes of vaccine candidate antigens that may have therapeutic efficacy across host species, disease manifestations, and delivery platforms.
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Banco de datos:
MEDLINE
Asunto principal:
Vacunas Bacterianas
/
Genoma Bacteriano
/
Vacunas de ADN
/
Chlamydophila
/
Antígenos Bacterianos
Tipo de estudio:
Diagnostic_studies
/
Guideline
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
Vaccine
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos