The HLDA8 blind panel: findings and conclusions.
J Immunol Methods
; 305(1): 75-83, 2005 Oct 20.
Article
en En
| MEDLINE
| ID: mdl-16126219
ABSTRACT
There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity.
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Banco de datos:
MEDLINE
Asunto principal:
Antígenos CD
/
Anticuerpos
/
Especificidad de Anticuerpos
/
Antígenos de Superficie
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
2005
Tipo del documento:
Article
País de afiliación:
Australia