Detection of domoic acid in rat serum and brain by direct competitive enzyme-linked immunosorbent assay (cELISA).
Anal Bioanal Chem
; 383(5): 783-6, 2005 Nov.
Article
en En
| MEDLINE
| ID: mdl-16158294
ABSTRACT
In 1987 a large-scale incident of human poisoning in Canada was traced to commercial mussels contaminated with domoic acid (DOM). Since then, routine screening of shellfish domoic acid content has been carried out using a variety of assays, with liquid chromatography using ultraviolet absorbance detection (LC-UV) or mass spectrometric detection (LC-MS) being the currently accepted standard methodologies. Recently, a highly specific competitive enzyme-linked immunosorbent assay (cELISA) has been developed for the detection and analysis of DOM in commercial shellfish, but its accuracy relative to LC methods has not been independently verified in mammalian tissues. In this study we demonstrate that measurement of rat serum DOM concentration by cELISA gives a good correlation (r2 = 0.993) across a broad range of concentrations when compared to LC-MS analysis, with only a small (15%) overestimation of sample DOM content. In addition, we have developed an extraction method for analysis of DOM in rat brain by cELISA which yields complete recovery across a range of sample dilutions.
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Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Encéfalo
/
Ensayo de Inmunoadsorción Enzimática
/
Cromatografía Liquida
/
Ácido Kaínico
/
Microquímica
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2005
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda