Comparative proteome analysis of breast cancer and adjacent normal breast tissues in human.
Genomics Proteomics Bioinformatics
; 4(3): 165-72, 2006 Aug.
Article
en En
| MEDLINE
| ID: mdl-17127214
ABSTRACT
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS), incorporated with online database searching, were performed to investigate differential proteins of breast cancer and adjacent normal breast tissues. Considering that serum albumin is abundantly presented in normal control samples, 15 differential spots detected in 11 out of 12 (91.7%) breast cancer samples were identified by online SIENA-2DPAGE database searching and MALDI-TOF/TOF-MS analysis. The results indicate that pathological changes of breast cancer are concerned with augmentation of substance metabolism, promotion of proteolytic activity, decline of activity of some inhibitors of enzymes, and so on. Some important proteins involved in the pathological process of breast cancer with changed expression may be useful biomarkers, such as alpha-1-antitrypsin, EF-1-beta, cathepsin D, TCTP, SMT3A, RPS12, and PSMA1, among which SMT3A, RPS12, and PSMA1 were first reported for breast cancer in this study.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Mama
/
Neoplasias de la Mama
/
Regulación Neoplásica de la Expresión Génica
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Proteómica
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Genomics Proteomics Bioinformatics
Asunto de la revista:
BIOQUIMICA
/
GENETICA
/
INFORMATICA MEDICA
Año:
2006
Tipo del documento:
Article
País de afiliación:
China