[Treatment of mouse liver metastasis by intraportal injection of Adv-p53].
Zhonghua Zhong Liu Za Zhi
; 29(11): 818-21, 2007 Nov.
Article
en Zh
| MEDLINE
| ID: mdl-18396637
ABSTRACT
OBJECTIVE:
To investigate the anti-tumor effect of intraportal administration of Adv-p53 in the treatment of the liver metastasis in mice.METHODS:
2 x 10(5) of MCA-205 cells were injected into the mouse portal vein to establish a murine liver metastasis model. The spleen was transpositioned subcutaneously to enable the administration of Adv-p53 continually into the portal system. Different doses of Adv-p53 were injected intraportally, while HBSS and Adv-CMV were injected intraportaly in the control group. Tumors in the liver were examined on day 21 after Adv-p53 administration.RESULTS:
The liver weight in the Adv-p53 treated mice on day 0 group (1.20 +/- 0.34 g) was significantly less than that in the Adv-CMV group (2.59 +/- 0.48 g, P < 0.05). The number of metastatic nodules in the Adv-p53 treated mice on day 0 group (9.0 +/- 9.9) was significantly less than that in the Adv-CMV group (57.1 +/- 11.3, P < 0.05), indicating that intraportal administration of Adv-p53 inhibited the formation of liver metastasis. This anti-tumor effect was in a dose-dependent manner. After the liver metastasis was formed, Adv-p53 was administered intraportally. The liver weight in the Adv-p53 treated mice on day 5 group (1.22 +/- 0.09 g) was significantly less than that in the Adv-CMV group (3.98 +/- 1.01 g , P < 0.05). The number of metastatic nodules in the Adv-p53 treaed mice on day 5 group (5.5 +/- 3.5) was significantly less than that in the Adv-CMV group (113.2 +/- 5.8, P < 0.05). Repeatedly intraportal administration of Adv-p53 could enhance this anti-tumor effect.CONCLUSION:
Local administration of Adv-p53 into the portal system would be a useful strategy for the liver metastasis treatment.
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Banco de datos:
MEDLINE
Asunto principal:
Terapia Genética
/
Adenoviridae
/
Proteína p53 Supresora de Tumor
/
Fibrosarcoma
/
Neoplasias Hepáticas Experimentales
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Zhong Liu Za Zhi
Año:
2007
Tipo del documento:
Article
País de afiliación:
China