Microtubule stabilizing effect of notch activation in primary cortical neurons.
Neuroscience
; 154(3): 946-52, 2008 Jun 26.
Article
en En
| MEDLINE
| ID: mdl-18495362
ABSTRACT
The appropriate level of microtubule stability is fundamental in neurons to assure correct polarity, migration, vesicles transport and to prevent axonal degeneration. In the present study, we have identified Notch pathway as an endogenous microtubule stabilizer. Stimulation of Notch receptors by exposure of mouse cortical neurons to the Notch ligand Jagged1 resulted in increased microtubule stability, as measured by using antibodies against post-translationally modified alpha tubulin, and changes in axonal morphology and branching, with varicosity loss, thicker neurites and enlarged growth cones. Similar effects were found after exposure of the cells to different doses of Taxol. However, contrary to Taxol, Jagged1 induced downregulation of the microtubule severing protein Spastin. We suggest that a fine-tuned manipulation of Notch signaling may represent a novel approach to modulate neuronal cytoskeleton plasticity.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Corteza Cerebral
/
Receptores Notch
/
Microtúbulos
/
Neuronas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Neuroscience
Año:
2008
Tipo del documento:
Article
País de afiliación:
Italia