[Sensitivity and specificity of the VIKIA Rota-Adeno immuno-chromatographic test (bioMérieux) and the ELISA IDEIA Rotavirus kit (Dako) compared to genotyping]. / Sensibilité et spécificité du test immuno-chromatographique VIKIA Rota-Adéno (bioMérieux) et du kit Elisa IDEIA Rotavirus (Dako) par rapport aux techniques de génotypage.
Pathol Biol (Paris)
; 57(1): 86-9, 2009 Feb.
Article
en Fr
| MEDLINE
| ID: mdl-18838230
ABSTRACT
The performances of two diagnostic tests for rotavirus infection in stool samples were evaluated during a prospective study in children of less than 36 months in child-care centers of Lyon from November 2004 to May 2005. The VIKIA Rota-Adeno immuno-chromatographic test (bioMérieux) and the ELISA IDEIA Rotavirus kit (Dako) were compared with a referral method, the genotyping. Fifty-seven stool samples were collected and analyzed by RT-PCR. The virus genome was detected in 29 samples. The most frequent genotypic combinations were G9P[8] with a prevalence of 75.9%. Sensitivity and specificity of the VIKIA Rota-Adeno test and the ELISA IDEIA Rotavirus kit were strictly comparable and very good 96.6% (83.0; 99.9) and 96.4% (81.6; 99.9), respectively. The immuno-chromatographic technique were in concordance with the ELISA tests in 93.6% of cases. Thus, the VIKIA Rota-Adeno test is a good alternative for the occasional analysis of stool samples in ambulatory practice.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Infecciones por Rotavirus
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Ensayo de Inmunoadsorción Enzimática
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Inmunoensayo
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Cromatografía de Afinidad
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Infecciones por Adenoviridae
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Gastroenteritis
Tipo de estudio:
Clinical_trials
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Diagnostic_studies
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Observational_studies
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Risk_factors_studies
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Screening_studies
Límite:
Child, preschool
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Female
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Humans
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Infant
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Male
País/Región como asunto:
Europa
Idioma:
Fr
Revista:
Pathol Biol (Paris)
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia