Insulin secretion from beta-cells is affected by deletion of nicotinamide nucleotide transhydrogenase.
Methods Enzymol
; 457: 451-80, 2009.
Article
en En
| MEDLINE
| ID: mdl-19426883
ABSTRACT
Nicotinamide nucleotide transhydrogenase (NNT) is an inner mitochondrial membrane transmembrane protein involved in regenerating NADPH, coupled with proton translocation across the inner membrane. We have shown that a defect in Nnt function in the mouse, and specifically within the beta-cell, leads to a reduction in insulin secretion. This chapter describes methods for examining Nnt function in the mouse. This includes generating in vivo models with point mutations and expression of Nnt by transgenesis, and making in vitro models, by silencing of gene expression. In addition, techniques are described to measure insulin secretion, calcium and hydrogen peroxide concentrations, membrane potential, and NNT activity. These approaches and techniques can also be applied to other genes of interest.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Insulina
/
Mitocondrias
/
NADP Transhidrogenasas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Methods Enzymol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Reino Unido